LOCUS       sequence-267196-        8809 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  constitutive expression of IRE1a from A. thaliana (AT2G17520; 
            upregulates UPR signaling).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8809)
  AUTHORS   Alcantara A, Seitner D, Navarrete F, Djamei A
  TITLE     A high-throughput screening method to identify proteins involved in 
            unfolded protein response of the endoplasmic reticulum in plants.
  JOURNAL   Plant Methods. 2020 Jan 21;16:4. doi: 10.1186/s13007-020-0552-3. 
            eCollection 2020.
  PUBMED    31988651
REFERENCE   2  (bases 1 to 8809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2020-01-21"; volume: "16:4. doi"; pages: " 
            10.1186/s13007-020-0552-3. eCollection 2020"
COMMENT     Sequence Label: p35S::IRE1a
FEATURES             Location/Qualifiers
     source          1..8809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    315..337
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     promoter        930..1275
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1218..1238
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    1303..1358
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     promoter        5550..5729
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             5771..6322
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     misc_feature    6609..6633
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(6724..7312)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6804..6823)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7483..8271)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(8562..8809,1..188)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 cctttcagat caacagcttg caaacacccc tcgctccggc aagtagttac agcaagtagt
       61 atgttcaatt agcttttcaa ttatgaatat atatatcaat tattggtcgc ccttggcttg
      121 tggacaatgc gctacgcgca ccggctccgc ccgtggacaa ccgcaagcgg ttgcccaccg
      181 tcgagcgcct ttgcccacaa cccggcggcc ggccgcaaca gatcgtttta taaatttttt
      241 tttttgaaaa agaaaaagcc cgaaaggcgg caacctctcg ggcttctgga tttccgatcc
      301 ccggaattag atcttggcag gatatattgt ggtgtaacgt tctcgaacta ggctcggacg
      361 aagtaagcgg ccgcaacctt aattaaacac ggcgcgccaa cagcgatcgc accaggtacc
      421 acctccgatc cattcggtcc ccagattagc cttttcaatt tcagaaagaa tgctaaccca
      481 cagatggtta gagaggctta cgcagcacgt ctcatcaaga cgatctaccc gagcaataat
      541 ctccaggaaa tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact
      601 aactgcatca agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta
      661 tggacgattc aaggcttgct tcacaaacca aggcaagtaa tagagattgg agtctctaaa
      721 aaggtagttc ccactgaatc aaaggccatg gagtcaaaga ttcaaataga ggacctaaca
      781 gaactcgccg taaagactgg cgaacagttc atacagagtc tcttacgact caatgacaag
      841 aagaaaatct tcgtcaacat ggtggagcac gacacacttg tctactccaa aaatatcaaa
      901 gatacagtct cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga
      961 aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag
     1021 gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc
     1081 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
     1141 gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg
     1201 gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt
     1261 catttggaga gaacacgggg gacgagctaa catattttta caacaattac caacaacaac
     1321 aaacaacaaa caacattaca attactattt acaattacag gctccatgcc gccgagatgt
     1381 cctttcctcc gccatctttt cttcttacta ctattactct caccatggat catgtctcct
     1441 tgcggcggcg ctgccgacga tgtgacgtat ccgatcgttc catctagtcc cggccgcaga
     1501 tcaattttgc agattcgtcg gtgagtttct tccatcctct gcatgtttaa aaatacttag
     1561 ttagtatttg attctggtga cactgtttta ggtatcatta gcattgtaaa cgtgatggac
     1621 tagtgattag gttttggtgg tggcaagaat tggtttgatt acattttgat agatgttttt
     1681 gattcttctc tatgtggtta ctggttgtga tgttatctgt cttctactca gtgaaccacc
     1741 aacagagcca aatacgaaac ttgttgttga tcgtgatggg aaggtgtttt taaagcagca
     1801 accgaaagaa acaccatact ggtccttttc aacaggatca ccaatgcact cgttatatca
     1861 ggctcctgct aataacaaca cagaaaatgc tactgagata actcgtcctc acataattgt
     1921 tgaatatctg aacaactcaa aggcggctac tactgtcgat ggctatcata attgggtatg
     1981 aagcatctct ctctttactt cattattact ggactttgtt tatcttaact ttgtttcggc
     2041 acatattcac tgcagacagt tcaggagttt tttaggcaga agccacttgt aacagatgat
     2101 ggtgtgactc ttggatcaga aacaactagt gcctatttgg ttgatggtag atcagggagg
     2161 ctgattcatg tttacaaatc aactggagat actaagatta ctaatgctct cgtgaagccc
     2221 gctagcactg aagattttgt gaatgaacct cttcttatca ggcgaacaga ttccaaactt
     2281 gagcatttta gtaaaaccac tgggaagctt gtctggaatt tgacggtttc tcacttcaga
     2341 gctgctttac tatgtgatcc tgttttcaat tctggctatg acctcggtcc taaacttcaa
     2401 actggaattt atatgccatt gttatgtgga tcacaaattg acgttcgtgg cccagaaatt
     2461 gttatcaggg tgctccatga tcagccaatg aatgtgaaaa tgctaccctc cccttcccta
     2521 aatcacttcg agagcgaaaa cagcatcatg ccttttggaa aagccagaga gtcaagaaag
     2581 ttacaagagc aacataagca gaaatataca tatttgtttg gacaatggtc accagtaaag
     2641 ttattggccc ctttagtgct tctaggtgtt gttgtctctg ttttcattaa aaagttttcg
     2701 tcgaggggca gtgatgttag tttgaaagct ggaccttcca aaaagaagaa gaaccgtaaa
     2761 tccgcgaagg atacaaatag gcagtcagtt cctcgtggcc aggatcagtt cgagctcatt
     2821 gaaggaggtc aaatgttact aggcttcaat aactttcaaa gtggtgcaac tgatgggaga
     2881 aagatcggta agttgtttct gtcaagtaaa gaaattgcaa agggaagtaa cggaactgtg
     2941 gtgtttgagg gtatttacga aggccggcct gtagctgtaa aacgccttgt tcgatctcat
     3001 catgaggttg ctttcaaaga gattcaaaac ctcattgcat cagaccaaca cacaaacatc
     3061 attcggtggt atggtgtgga gtatgatcaa gattttgtat acctttctct agagcgttgc
     3121 acctgcagtt tagatgatct aatcaaatcc tatttagagt tttctatgac aaaagtattg
     3181 gaaaacaacg attctactga aggagtggct gcttataaga tccaacttga ttctttggag
     3241 ggagttatca aaggaaacaa cttttggaaa gtggggggtc acccgtcacc tctcatgctt
     3301 aaactaatga ggttaatttg gacttcgtac ttttttatat atgtcataat attctttgat
     3361 taagtgatgt tctatttgaa catcaaaggt agctatggtt ctgataagtc taggctatgt
     3421 gatacaataa acagtagaat tagttcaaac cttgtttact gatttactgt ttaaacttat
     3481 atttttcaga ttgtttgtat tgtgatgcag ggatatagtt tgtggaatcg tccacttaca
     3541 cgaattggga atagttcatc gggacttgaa gccacaaaat gtgttaatta gtaaagatat
     3601 gactttgagt gcaaaactgt ccgatatggg cattagcaag cgtatgtcca gagatatgtc
     3661 atcattgggt caccttgcca caggtaagtt gttttttatt atcttttttt atgcccaaaa
     3721 tattggattt gtaacacttt caaaaatagg actgattgat ctcgcaaaac aaaactaaca
     3781 ctagagaaaa actacataat gtcataagtt ggtcaataga atgcatgtta gtgtctgaac
     3841 gcaatgtatg atcgtttgtt gaatatgtgt tatgtttatg aaatgagttt caacccacat
     3901 ttttaaagta tctgctttaa aaactacagg ttctggcagt tcgggctggc aagcaccaga
     3961 gcagcttcta caaggtcgtc aaactcgtgc agtcgatatg tttagtttag gttgtgtcat
     4021 cttttacact ataaccggtt gtaagcatcc ctttggtgat gatcttgaac gagatgtcaa
     4081 tattgtgaag aacaaagtcg acctatttct agtagaacac gttccagaag cttcagacct
     4141 catatcccgt ttgttaaacc cagaccctga tttacggtaa tgttactttc ctctctgatg
     4201 attaaaatcc actcgaaaaa gcctaatctc ttatacactg tttgctcttt gcagtcctag
     4261 cgcaactgaa gtgctactcc atcctatgtt ttggaactca gagatgagac tgtctttcct
     4321 tcgtgatgct agtgaccgag ttgagcttga aaatagagaa gctgattctg aaatcttgaa
     4381 agcaatggag agtacagctc cagtggctat aggagggaaa tgggatgaaa agctagaacc
     4441 tgttttcatc acaaacatcg ggcgctacag gcgttacaaa tacgatagca tccgtgactt
     4501 gttacgagtc atcaggaaca aactgaatca tcatcgagaa cttcctccgg aaattcaggt
     4561 tcatcttata attgaatccc tcaaacaccg ttacattaca ctcacgggct taaactgatt
     4621 attttgaaac ttttgatgtc ttaggaactt gtcggaacag ttccagaagg attcgacgag
     4681 tactttgctg tacggtttcc gaaattgttg attgaagtct atagagtcat ctctctgcat
     4741 tgtagggaag aagaagtatt cagaaagtac ttcaaatgcg acatcatcta atcaggtgga
     4801 tcctagataa cctttacctt cattccctgt aaattggtac cctgcatctc gtctctgtta
     4861 tgcttaagaa gttcaatgtt tcgtttcatg taaaactttg gtggtttgtg ttttggggcc
     4921 ttgtataatc cctgatgaat aagtgttcta ctatgtttcc gttcctgtta tctctttctt
     4981 tctaatgaca agtcgaactt cttctttatc atcgcttcgt ttttattatc tgtgcttctt
     5041 ttgtttaata cgcctgcaaa gtgactcgac tctgtttagt gcagttctgc gaaacttgta
     5101 aatagtccaa ttgttggcct ctagtaatag atgtagcgaa agtgttgagc tgttgggttc
     5161 taaggatggc ttgaacatgt taatctttta ggttctgagt atgatgaaca ttcgttgttg
     5221 ctaagaaatg cctgtaatgt cccacaaatg tagaaaatgg ttcgtacctt tgtccaagca
     5281 ttgatatgtc tgatgagagg aaactgcaag atactgagct tggtttaacg aaggagaggc
     5341 agtttcttcc ttccaaagca tttcatttga caatgccttg atcatcttaa gtagagtttc
     5401 tgttgtggaa agtttgaaac tttgaagaaa cgactctcaa gtaaattgat gatcacaagt
     5461 gaaagtgtat gttactagag aattaaggga gtcacgttat gacccccgcc gatgacgcgg
     5521 gacaagccgt tttacgtttg gaactgacag aaccgcaacg ttgaaggagc cactgagccg
     5581 cgggtttctg gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa
     5641 aagtcgccta aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg
     5701 ttccataaat tcccctcggt atccaattag agtctcatat tcactctcaa ctcgatcgag
     5761 gggatctacc atgagcccag aacgacgccc ggccgacatc cgccgtgcca ccgaggcgga
     5821 catgccggcg gtctgcacca tcgtcaacca ctacatcgag acaagcacgg tcaacttccg
     5881 taccgagccg caggaaccgc aggagtggac ggacgacctc gtccgtctgc gggagcgcta
     5941 tccctggctc gtcgccgagg tggacggcga ggtcgccggc atcgcctacg cgggtccctg
     6001 gaaggcacgc aacgcctacg actggacggc cgagtccacc gtgtacgtct ccccccgcca
     6061 tcagcggacg ggactgggct ccacgctcta cacccacctg ctgaagtccc tggaggcaca
     6121 gggcttcaag agcgtggtcg ctgtcatcgg gctgcccaac gacccgagcg tgcgcatgca
     6181 cgaggcgctc ggatatgccc cccgcggcat gctgcgggcg gccggcttca agcacgggaa
     6241 ctggcatgac gtgggtttct ggcagctgga cttcagcctg ccggtgccgc cccgtccggt
     6301 cctgcccgtc accgaaatct gatgacccct agagtcaagc agatcgttca aacatttggc
     6361 aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc
     6421 tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat
     6481 gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
     6541 agcgcgcaaa ctaggataaa gtattctaga ccaagcatgc acagctggcg ttctcagcca
     6601 gatccccttg acaggatata ttggcgggta aactaagtcg ctgtatgtgt ttgtttgaga
     6661 tctcatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     6721 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     6781 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     6841 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     6901 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     6961 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7021 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     7081 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     7141 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     7201 taccttcgga agaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     7261 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7321 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     7381 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     7441 taaatcaatc taaagtatat atgtgtaaca ttggtctagt gattatttgc cgactacctt
     7501 ggtgatctcg cctttcacgt agtgaacaaa ttcttccaac tgatctgcgc gcgaggccaa
     7561 gcgatcttct tgtccaagat aagcctgcct agcttcaagt atgacgggct gatactgggc
     7621 cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt tgccggttac
     7681 tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc cagcccagtc
     7741 gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat cctgttcagg
     7801 aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat gttctcttgc
     7861 ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag
     7921 aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat aacgccacgg
     7981 aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct cgctctctcc
     8041 aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag
     8101 ccttacggtc accgtaacca gcaaatcaat atcactgtgt ggcttcaggc cgccatccac
     8161 tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct cgatgacgcc
     8221 aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctca taacacccct
     8281 tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg
     8341 tgcaatgtaa catcagagat tttgagacac aacgtggctt tgttgaataa atcgaacttt
     8401 tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa
     8461 gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc
     8521 cctcactttc tggctggatg atggggcgat tcaggcgatc cccatccaac agcccgccgt
     8581 cgagcgggct tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc
     8641 gctaatgccc cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca
     8701 cgtgaaatcg ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata
     8761 aggtcacgtg aaatcgctaa tcaaaaaggc acgtgagaac gctaatagc
//
