LOCUS       sequence-267197-        5890 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  constitutive expression of HY5 from A. thaliana (AT5G11260; 
            downregulates UPR signaling).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5890)
  AUTHORS   Alcantara A, Seitner D, Navarrete F, Djamei A
  TITLE     A high-throughput screening method to identify proteins involved in 
            unfolded protein response of the endoplasmic reticulum in plants.
  JOURNAL   Plant Methods. 2020 Jan 21;16:4. doi: 10.1186/s13007-020-0552-3. 
            eCollection 2020.
  PUBMED    31988651
REFERENCE   2  (bases 1 to 5890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2020-01-21"; volume: "16:4. doi"; pages: " 
            10.1186/s13007-020-0552-3. eCollection 2020"
COMMENT     Sequence Label: p35S::HY5
FEATURES             Location/Qualifiers
     source          1..5890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    296..318
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     promoter        911..1256
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1199..1219
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    1284..1339
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     promoter        2612..2791
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2833..3384
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     misc_feature    3671..3695
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(3786..4374)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3866..3885)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4545..5333)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(5624..5890,1..169)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 gcaaacaccc ctcgctccgg caagtagtta cagcaagtag tatgttcaat tagcttttca
       61 attatgaata tatatatcaa ttattggtcg cccttggctt gtggacaatg cgctacgcgc
      121 accggctccg cccgtggaca accgcaagcg gttgcccacc gtcgagcgcc tttgcccaca
      181 acccggcggc cggccgcaac agatcgtttt ataaattttt ttttttgaaa aagaaaaagc
      241 ccgaaaggcg gcaacctctc gggcttctgg atttccgatc cccggaatta gatcttggca
      301 ggatatattg tggtgtaacg ttctcgaact aggctcggac gaagtaagcg gccgcaacct
      361 taattaaaca cggcgcgcca acagcgatcg caccaggtac cacctccgat ccattcggtc
      421 cccagattag ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt
      481 acgcagcacg tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc
      541 ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag
      601 agaaagatat atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc
      661 ttcacaaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat
      721 caaaggccat ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg
      781 gcgaacagtt catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca
      841 tggtggagca cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc
      901 aaagggcaat tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt
      961 gcccagctat ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat
     1021 gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca
     1081 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt
     1141 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact
     1201 atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg
     1261 ggacgagcta acatattttt acaacaatta ccaacaacaa caaacaacaa acaacattac
     1321 aattactatt tacaattaca ggctccatgc aggaacaagc gactagctct ttagctgcaa
     1381 gctctttacc atcaagcagc gagaggtcat caagctctgc tccacatttg gagatcaaag
     1441 aaggaattga aagcgatgag gagatacggc gagtgccgga gtttggagga gaagctgtcg
     1501 gaaaagaaac ttccggtaga gaatctggat cggcgaccgg tcaggagcgg acacaggcga
     1561 ctgtcggaga aagtcaaagg aagcgaggga ggacaccggc ggagaaagag aacaagcggc
     1621 tgaagaggtt gttgaggaac agagtttcag ctcagcaagc aagagagagg aaaaaggctt
     1681 acttgagcga gttggaaaac agagtgaaag acttggagaa caaaaactct gaacttgaag
     1741 agcgactctc tactcttcag aacgagaacc agatgcttag acatattctg aagaacacaa
     1801 caggaaacaa gagaggaggt ggtggtggtt ctaatgctga tgcaagcctt tgatcaggtg
     1861 gatcctagat aacctttacc ttcattccct gtaaattggt accctgcatc tcgtctctgt
     1921 tatgcttaag aagttcaatg tttcgtttca tgtaaaactt tggtggtttg tgttttgggg
     1981 ccttgtataa tccctgatga ataagtgttc tactatgttt ccgttcctgt tatctctttc
     2041 tttctaatga caagtcgaac ttcttcttta tcatcgcttc gtttttatta tctgtgcttc
     2101 ttttgtttaa tacgcctgca aagtgactcg actctgttta gtgcagttct gcgaaacttg
     2161 taaatagtcc aattgttggc ctctagtaat agatgtagcg aaagtgttga gctgttgggt
     2221 tctaaggatg gcttgaacat gttaatcttt taggttctga gtatgatgaa cattcgttgt
     2281 tgctaagaaa tgcctgtaat gtcccacaaa tgtagaaaat ggttcgtacc tttgtccaag
     2341 cattgatatg tctgatgaga ggaaactgca agatactgag cttggtttaa cgaaggagag
     2401 gcagtttctt ccttccaaag catttcattt gacaatgcct tgatcatctt aagtagagtt
     2461 tctgttgtgg aaagtttgaa actttgaaga aacgactctc aagtaaattg atgatcacaa
     2521 gtgaaagtgt atgttactag agaattaagg gagtcacgtt atgacccccg ccgatgacgc
     2581 gggacaagcc gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactgagc
     2641 cgcgggtttc tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc
     2701 aaaagtcgcc taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga
     2761 cgttccataa attcccctcg gtatccaatt agagtctcat attcactctc aactcgatcg
     2821 aggggatcta ccatgagccc agaacgacgc ccggccgaca tccgccgtgc caccgaggcg
     2881 gacatgccgg cggtctgcac catcgtcaac cactacatcg agacaagcac ggtcaacttc
     2941 cgtaccgagc cgcaggaacc gcaggagtgg acggacgacc tcgtccgtct gcgggagcgc
     3001 tatccctggc tcgtcgccga ggtggacggc gaggtcgccg gcatcgccta cgcgggtccc
     3061 tggaaggcac gcaacgccta cgactggacg gccgagtcca ccgtgtacgt ctccccccgc
     3121 catcagcgga cgggactggg ctccacgctc tacacccacc tgctgaagtc cctggaggca
     3181 cagggcttca agagcgtggt cgctgtcatc gggctgccca acgacccgag cgtgcgcatg
     3241 cacgaggcgc tcggatatgc cccccgcggc atgctgcggg cggccggctt caagcacggg
     3301 aactggcatg acgtgggttt ctggcagctg gacttcagcc tgccggtgcc gccccgtccg
     3361 gtcctgcccg tcaccgaaat ctgatgaccc ctagagtcaa gcagatcgtt caaacatttg
     3421 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     3481 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     3541 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     3601 atagcgcgca aactaggata aagtattcta gaccaagcat gcacagctgg cgttctcagc
     3661 cagatcccct tgacaggata tattggcggg taaactaagt cgctgtatgt gtttgtttga
     3721 gatctcatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     3781 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     3841 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     3901 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3961 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     4021 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4081 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     4141 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     4201 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     4261 gttaccttcg gaagaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     4321 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     4381 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     4441 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     4501 tttaaatcaa tctaaagtat atatgtgtaa cattggtcta gtgattattt gccgactacc
     4561 ttggtgatct cgcctttcac gtagtgaaca aattcttcca actgatctgc gcgcgaggcc
     4621 aagcgatctt cttgtccaag ataagcctgc ctagcttcaa gtatgacggg ctgatactgg
     4681 gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt
     4741 actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag
     4801 tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca
     4861 ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt
     4921 gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca
     4981 agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac
     5041 ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct
     5101 ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca
     5161 agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc
     5221 actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg
     5281 ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttccct cataacaccc
     5341 cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct
     5401 tgtgcaatgt aacatcagag attttgagac acaacgtggc tttgttgaat aaatcgaact
     5461 tttgctgagt tgaaggatca gatcacgcat cttcccgaca acgcagaccg ttccgtggca
     5521 aagcaaaagt tcaaaatcac caactggtcc acctacaaca aagctctcat caaccgtggc
     5581 tccctcactt tctggctgga tgatggggcg attcaggcga tccccatcca acagcccgcc
     5641 gtcgagcggg cttttttatc cccggaagcc tgtggataga gggtagttat ccacgtgaaa
     5701 ccgctaatgc cccgcaaagc cttgattcac ggggctttcc ggcccgctcc aaaaactatc
     5761 cacgtgaaat cgctaatcag ggtacgtgaa atcgctaatc ggagtacgtg aaatcgctaa
     5821 taaggtcacg tgaaatcgct aatcaaaaag gcacgtgaga acgctaatag ccctttcaga
     5881 tcaacagctt
//
