LOCUS       sequence-267201-       10158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of BRSK2 (human) tagged with N-terminal 
            Flag for generating protein-protein networks using fusion tag 
            affinity-based proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10158)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHAGE-CMV-Flag-BRSK2_IDG-K
FEATURES             Location/Qualifiers
     source          1..10158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2227..2530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2531..2734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2685..2705
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2731..2755
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2826..2835
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2835..2858
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    2868..2892
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4927..4951)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          5025..5254
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5237..5256
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(5327..5901,5902..5904,5905..5913)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5494..5511)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5721..5740
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5933..6532
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5933..5952)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6429..6449
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6547..7135
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6600..6620)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7018..7029)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7210..7443
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7562..7580)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7648..7752
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7753..7821,7822..8613)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7971..7990)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8784..9372
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9273..9292
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9526..9543
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9784..9805
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9820..9825,9826..9843,9844..9850)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9858..9874
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9863..9885
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9882..9898
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9882..9898
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9948..9967
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
     2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2701 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     2761 gagacgccat ccacgctgtt ttgacctcca tagaagacac cggcggccat accggtcagc
     2821 tcgaggccac catggactac aaggacgacg acgacaaggg cggatcaaca agtttgtaca
     2881 aaaaagcaga agggccgtca aggcccacca tgacatcgac ggggaaggac ggcggcgcgc
     2941 agcacgcgca gtatgttggg ccctaccggc tggagaagac gctgggcaag gggcagacag
     3001 gtctggtgaa gctgggggtt cactgcgtca cctgccagaa ggtggccatc aagatcgtca
     3061 accgtgagaa gctcagcgag tcggtgctga tgaaggtgga gcgggagatc gcgatcctga
     3121 agctcattga gcacccccac gtcctaaagc tgcacgacgt ttatgaaaac aaaaaatatt
     3181 tgtacctggt gctagaacac gtgtcaggtg gtgagctctt cgactacctg gtgaagaagg
     3241 ggaggctgac gcctaaggag gctcggaagt tcttccggca gatcatctct gcgctggact
     3301 tctgccacag ccactccata tgccacaggg atctgaaacc tgaaaacctc ctgctggacg
     3361 agaagaacaa catccgcatc gcagactttg gcatggcgtc cctgcaggtt ggcgacagcc
     3421 tgttggagac cagctgtggg tccccccact acgcctgccc cgaggtgatc cggggggaga
     3481 agtatgacgg ccggaaggcg gacgtgtgga gctgcggcgt catcctgttc gccttgctgg
     3541 tgggggctct gcccttcgac gatgacaact tgcgacagct gctggagaag gtgaagcggg
     3601 gcgtgttcca catgccgcac tttatcccgc ccgactgcca gagtctgcta cggggcatga
     3661 tcgaggtgga cgccgcacgc cgcctcacgc tagagcacat tcagaaacac atatggtata
     3721 tagggggcaa gaatgagccc gaaccagagc agcccattcc tcgcaaggtg cagatccgct
     3781 cgctgcccag cctggaggac atcgaccccg acgtgctgga cagcatgcac tcactgggct
     3841 gcttccgaga ccgcaacaag ctgctgcagg acctgctgtc cgaggaggag aaccaggaga
     3901 agatgattta cttcctcctc ctggaccgga aagaaaggta cccgagccag gaggatgagg
     3961 acctgccccc ccggaacgag atagaccctc cccggaagcg tgtggactcc ccgatgctga
     4021 accggcacgg caagcggcgg ccagaacgca aatccatgga ggtgctcagc gtgacggacg
     4081 gcggctcccc ggtgcctgcg cggcgggcca ttgagatggc ccagcacggc cagaggtctc
     4141 ggtccatcag cggtgcctcc tcaggccttt ccaccagccc actcagcagc ccccgggtga
     4201 cccctcaccc ctcaccaagg ggcagtcccc tccccacccc caaggggaca cctgtccaca
     4261 cgccaaagga gagcccggct ggcacgccca accccacgcc cccgtccagc cccagcgtcg
     4321 gaggggtgcc ctggagggcg cggctcaact ccatcaagaa cagctttctg ggctcacccc
     4381 gcttccaccg ccggaaactg caagttccga cgccggagga gatgtccaac ctgacaccag
     4441 agtcgtcccc agagctggcg aagaagtcct ggtttgggaa cttcatcagc ctggagaagg
     4501 aggagcagat cttcgtggtc atcaaagaca aacctctgag ctccatcaag gctgacatcg
     4561 tgcacgcctt cctgtcgatt cccagtctca gccacagcgt catctcccaa acgagcttcc
     4621 gggccgagta caaggccacg ggggggccag ccgtgttcca gaagccggtc aagttccagg
     4681 ttgatatcac ctacacggag ggtggggagg cgcagaagga gaacggcatc tactccgtca
     4741 ccttcaccct gctctcaggc cccagccgtc gcttcaagag ggtggtggag accatccagg
     4801 cccagctgct gagcacacac gacccgcctg cggcccagca cttgtcagac accactaact
     4861 gtatggaaat gatgacgggg cggctttcca aatgtggaat tatcccgaaa agttcaggcc
     4921 tcatgggccc agctttcttg tacaaagtgg ttgattaaga tccactagta acggccgcca
     4981 gtgtgctgga attaattcgc tgtctgcgag ggccagctgt tggggtgagt actccctctc
     5041 aaaagcgggc atgacttctg cgctaagatt gtcagtttcc aaaaacgagg aggatttgat
     5101 attcacctgg cccgcggtga tgcctttgag ggtggccgcg tccatctggt cagaaaagac
     5161 aatctttttg ttgtcaagct tgaggtgtgg caggcttgag atctggccat acacttgagt
     5221 gacaatgaca tccactttgc ctttctctcc acaggtgtcc actcccaggt ccaactgcag
     5281 gtcgagcatg catctagggc ggccaattcc gcccctctcc cccccccccc tctccctccc
     5341 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     5401 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     5461 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     5521 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     5581 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     5641 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     5701 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     5761 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     5821 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     5881 tttgaaaaac acgatgataa gcttgccaca acccacaagg agacgacctt ccatgaccga
     5941 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct
     6001 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat
     6061 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     6121 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     6181 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     6241 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     6301 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     6361 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     6421 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     6481 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gatatcgata
     6541 gatcctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     6601 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     6661 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     6721 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     6781 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     6841 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     6901 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     6961 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     7021 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     7081 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag
     7141 atcctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     7201 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     7261 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7321 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7381 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7441 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     7501 cagagagtga gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag
     7561 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     7621 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     7681 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     7741 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     7801 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     7861 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     7921 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     7981 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     8041 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     8101 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     8161 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     8221 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     8281 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     8341 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     8401 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     8461 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     8521 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     8581 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     8641 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     8701 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     8761 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     8821 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     8881 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     8941 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     9001 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     9061 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     9121 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     9181 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     9241 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     9301 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     9361 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     9421 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     9481 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     9541 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     9601 taatgcagca agctcatggc tgactaattt ttttttattt atgcagaggc cgaggccgcc
     9661 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     9721 aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     9781 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     9841 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat
     9901 gattacgaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     9961 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
    10021 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
    10081 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
    10141 tactacaaac ttagtagt
//
