LOCUS       sequence-267204-       10437 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of TLK2 (human) tagged with N-terminal 
            Flag for generating protein-protein networks using fusion tag 
            affinity-based proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10437)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHAGE-CMV-Flag-TLK2_IDG-K
FEATURES             Location/Qualifiers
     source          1..10437
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2227..2530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2531..2734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2685..2705
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2731..2755
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2826..2835
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2835..2858
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    2868..2892
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(3470..3481)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(5206..5230)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          5304..5533
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5516..5535
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    5606..6179
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5773..5790)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6000..6019
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6212..6811
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6212..6231)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6708..6728
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6826..7414
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6879..6899)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7297..7308)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7489..7722
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7841..7859)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7927..8031
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8032..8100,8101..8892)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8250..8269)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9063..9651
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9552..9571
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9805..9822
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10063..10084
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10099..10104,10105..10122,10123..10129)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10137..10153
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10142..10164
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10161..10177
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10161..10177
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10227..10246
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
     2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2701 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     2761 gagacgccat ccacgctgtt ttgacctcca tagaagacac cggcggccat accggtcagc
     2821 tcgaggccac catggactac aaggacgacg acgacaaggg cggatcaaca agtttgtaca
     2881 aaaaagttgg catggaagaa ttgcatagcc tggacccacg acggcaggaa ttattggagg
     2941 ccaggtttac tggagtaggt gttagtaagg gaccacttaa tagtgagtct tccaaccaga
     3001 gcttgtgcag cgtcggatcc ttgagtgata aagaagtaga gactcccgag aaaaagcaga
     3061 atgaccagcg aaatcggaaa agaaaagctg aaccatatga aactagccaa gggaaaggca
     3121 ctcctagggg acataaaatt agtgattact ttgagtttgc tgggggaagc gcgccaggaa
     3181 ccagccctgg cagaagtgtt ccaccagttg cacgatcctc accgcaacat tccttatcca
     3241 atcccttacc gcgacgagta gaacagcccc tctatggttt agatggcagt gctgcaaagg
     3301 aggcaacgga ggagcagtct gctctgccaa ccctcatgtc agtgatgcta gcaaaacctc
     3361 ggcttgacac agagcagctg gcgcaaaggg gagctggcct ctgcttcact tttgtttcag
     3421 ctcagcaaaa cagtccctca tctacgggat ctggcaacac agagcattcc tgcagctccc
     3481 aaaaacagat ctccatccag cacagacaga cccagtccga cctcacaata gaaaaaatat
     3541 ctgcactaga aaacagtaag aattctgact tagagaagaa ggagggaaga atagatgatt
     3601 tattaagagc caactgtgat ttgagacggc agattgatga acagcaaaag atgctagaga
     3661 aatacaagga acgattaaat agatgtgtga caatgagcaa gaaactcctt atagaaaagt
     3721 caaaacaaga gaagatggcg tgtagagata agagcatgca agaccgcttg agactgggcc
     3781 actttactac tgtccgacac ggagcctcat ttactgaaca gtggacagat ggttatgctt
     3841 ttcagaatct tatcaagcaa caggaaagga taaattcaca gagggaagag atagaaagac
     3901 aacggaaaat gttagcaaag cggaaacctc ctgccatggg tcaggcccct cctgcaacca
     3961 atgagcagaa acagcggaaa agcaagacca atggagctga aaatgaaacg ccctcttctg
     4021 ggaatacaga gctaaaggat acagccccag ccttaggagc ccacagttta cttaggttaa
     4081 cgttagcaga ataccatgaa caagaagaaa tcttcaaact cagattaggt catcttaaaa
     4141 aggaggaagc agagatccag gcagagctgg agagactaga aagggttaga aatctacata
     4201 tcagggaact aaaaaggata cataatgaag ataattcaca atttaaagat catccaacgc
     4261 taaatgacag atatttgttg ttacatcttt tgggtagagg aggtttcagt gaagtttaca
     4321 aggcatttga tctaacagag caaagatacg tagctgtgaa aattcaccag ttaaataaaa
     4381 actggagaga tgagaaaaag gagaattacc acaagcatgc atgtagggaa taccggattc
     4441 ataaagagct ggatcatccc agaatagtta agctgtatga ttacttttca ctggatactg
     4501 actcgttttg tacagtatta gaatactgtg agggaaatga tctggacttc tacctgaaac
     4561 agcacaaatt aatgtcggag aaagaggccc ggtccattat catgcagatt gtgaatgctt
     4621 taaagtactt aaatgaaata aaacctccca tcatacacta tgacctcaaa ccaggtaata
     4681 ttcttttagt aaatggtaca gcgtgtggag agataaaaat tacagatttt ggtctttcga
     4741 agatcatgga tgatgatagc tacaattcag tggatggcat ggagctaaca tcacaaggtg
     4801 ctggtactta ttggtattta ccaccagagt gttttgtggt tgggaaagaa ccaccaaaga
     4861 tctcaaataa agttgatgtg tggtcggtgg gtgtgatctt ctatcagtgt ctttatggaa
     4921 ggaagccttt tggccataac cagtctcagc aagacatcct acaagagaat acgattctta
     4981 aagctactga agtgcagttc ccgccaaagc cagtagtaac acctgaagca aaggcgttta
     5041 ttcgacgatg cttggcctac cgaaaggagg accgcattga tgtccagcag ctggcctgtg
     5101 atccctactt gttgcctcac atccgaaagt cagtctctac aagtagccct gctggagctg
     5161 ctattgcatc aacctctggg gcgtccaata acagttcttc taattgccca actttcttgt
     5221 acaaagtggt tgattaagat ccactagtaa cggccgccag tgtgctggaa ttaattcgct
     5281 gtctgcgagg gccagctgtt ggggtgagta ctccctctca aaagcgggca tgacttctgc
     5341 gctaagattg tcagtttcca aaaacgagga ggatttgata ttcacctggc ccgcggtgat
     5401 gcctttgagg gtggccgcgt ccatctggtc agaaaagaca atctttttgt tgtcaagctt
     5461 gaggtgtggc aggcttgaga tctggccata cacttgagtg acaatgacat ccactttgcc
     5521 tttctctcca caggtgtcca ctcccaggtc caactgcagg tcgagcatgc atctagggcg
     5581 gccaattccg cccctctccc ccccccccct ctccnnnccc cccccctaac gttactggcc
     5641 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     5701 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     5761 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     5821 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     5881 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     5941 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     6001 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
     6061 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
     6121 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataag
     6181 cttgccacaa cccacaagga gacgaccttc catgaccgag tacaagccca cggtgcgcct
     6241 cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta
     6301 ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca
     6361 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     6421 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     6481 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     6541 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     6601 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     6661 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     6721 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     6781 ctggtgcatg acccgcaagc ccggtgcctg atatcgatag atcctaatca acctctggat
     6841 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     6901 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     6961 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     7021 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     7081 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     7141 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     7201 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     7261 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     7321 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     7381 acgagtcgga tctccctttg ggccgcctcc ccgcctgaga tcctttaaga ccaatgactt
     7441 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     7501 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     7561 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7621 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7681 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     7741 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggcccgggt
     7801 taattaagga aagggctaga tcattcttga agacgaaagg gcctcgtgat acgcctattt
     7861 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     7921 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     7981 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     8041 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     8101 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     8161 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     8221 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     8281 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     8341 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     8401 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     8461 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     8521 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     8581 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     8641 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     8701 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     8761 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     8821 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     8881 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     8941 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     9001 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     9061 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     9121 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     9181 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     9241 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     9301 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     9361 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     9421 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     9481 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     9541 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     9601 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     9661 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     9721 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     9781 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     9841 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa gctcatggct
     9901 gactaatttt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     9961 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc cgtggcacga
    10021 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
    10081 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
    10141 gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt tcacaaataa
    10201 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
    10261 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
    10321 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
    10381 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagt
//
