LOCUS       sequence-267208-       12591 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of CDK12 (human) tagged with N-terminal 
            Flag for generating protein-protein networks using fusion tag 
            affinity-based proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12591)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 12591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHAGE-CMV-Flag-CDK12_IDG-K
FEATURES             Location/Qualifiers
     source          1..12591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2227..2530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2531..2734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2685..2705
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2731..2755
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2826..2835
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2835..2858
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    2868..2892
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7360..7384)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          7458..7687
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     7670..7689
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(7760..8334,8335..8337,8338..8346)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7927..7944)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8154..8173
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8366..8965
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8366..8385)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8862..8882
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8980..9568
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9033..9053)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9451..9462)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9643..9876
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9995..10013)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10081..10185
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10186..10254,10255..11046)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10404..10423)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11217..11805
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11706..11725
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11959..11976
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12217..12238
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12253..12258,12259..12276,12277..12283)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12291..12307
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12296..12318
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12315..12331
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12315..12331
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12381..12400
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
     2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2701 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     2761 gagacgccat ccacgctgtt ttgacctcca tagaagacac cggcggccat accggtcagc
     2821 tcgaggccac catggactac aaggacgacg acgacaaggg cggatcaaca agtttgtaca
     2881 aaaaagcagg cttagccacc atgcccaatt cagagagaca tgggggcaag aaggacggga
     2941 gtggaggagc ttctggaact ttgcagccgt catcgggagg cggcagctct aacagcagag
     3001 agcgtcaccg cttggtatcg aagcacaagc ggcataagtc caaacactcc aaagacatgg
     3061 ggttggtgac ccccgaagca gcatccctgg gcacagttat caaacctttg gtggagtatg
     3121 atgatatcag ctctgattcc gacaccttct ccgatgacat ggccttcaaa ctagaccgaa
     3181 gggagaacga cgaacgtcgt ggatcagatc ggagcgaccg cctgcacaaa catcgtcacc
     3241 accagcacag gcgttcccgg gacttactaa aagctaaaca gaccgaaaaa gaaaaaagcc
     3301 aagaagtctc cagcaagtcg ggatcgatga aggaccggat atcgggaagt tcaaagcgtt
     3361 cgaatgagga gactgatgac tatgggaagg cgcaggtagc caaaagcagc agcaaggaat
     3421 ccaggtcatc caagctccac aaggagaaga ccaggaaaga acgggagctg aagtctgggc
     3481 acaaagaccg gagtaaaagt catcgaaaaa gggaaacacc caaaagttac aaaacagtgg
     3541 acagcccaaa acggagatcc aggagccccc acaggaagtg gtctgacagc tccaaacaag
     3601 atgatagccc ctcgggagct tcttatggcc aagattatga ccttagtccc tcacgatctc
     3661 atacctcgag caattatgac tcctacaaga aaagtcctgg aagtacctcg agaaggcagt
     3721 cggtcagtcc cccttacaag gagccttcgg cctaccagtc cagcacccgg tcaccgagcc
     3781 cctacagtag gcgacagaga tctgtcagtc cctatagcag gagacggtcg tccagctacg
     3841 aaagaagtgg ctcttacagc gggcgatcac ccagtcccta tggtcgaagg cggtccagca
     3901 gccctttcct gagcaagcgg tctctgagtc ggagtccact ccccagtagg aaatccatga
     3961 agtccagaag tagaagtcct gcatattcaa gacattcatc ttctcatagt aaaaagaaga
     4021 gatccagttc acgcagtcgt cattccagta tctcacctgt caggcttcca cttaattcca
     4081 gtctgggagc tgaactcagt aggaaaaaga aggaaagagc agctgctgct gctgcagcaa
     4141 agatggatgg aaaggagtcc aagggttcac ctgtattttt gcctagaaaa gagaacagtt
     4201 cagtagaggc taaggattca ggtttggagt ctaaaaagtt acccagaagt gtaaaattgg
     4261 aaaaatctgc cccagatact gaactggtga atgtaacaca tctaaacaca gaggtaaaaa
     4321 attcttcaga tacagggaaa gtaaagttgg atgagaactc cgagaagcat cttgttaaag
     4381 atttgaaagc acagggaaca agagactcta aacccatagc actgaaagag gagattgtta
     4441 ctccaaagga gacagaaaca tcagaaaagg agacccctcc acctcttccc acaattgctt
     4501 ctcccccacc ccctctacca actactaccc ctccacctca gacaccccct ttgccacctt
     4561 tgcctccaat accagctctt ccacagcaac cacctctgcc tccttctcag ccagcattta
     4621 gtcaggttcc tgcttccagt acttcaactt tgcccccttc tactcactca aagacatctg
     4681 ctgtgtcctc tcaggcaaat tctcagcccc ctgtacaggt ttctgtgaag actcaagtat
     4741 ctgtaacagc tgctattcca cacctgaaaa cttcaacgtt gcctcctttg cccctcccac
     4801 ccttattacc tggagatgat gacatggata gtccaaaaga aactcttcct tcaaaacctg
     4861 tgaagaaaga gaaggaacag aggacacgtc acttactcac agaccttcct ctccctccag
     4921 agctccctgg tggagatctg tctcccccag actctccaga accaaaggca atcacaccac
     4981 ctcagcaacc atataaaaag agaccaaaaa tttgttgtcc tcgttatgga gaaagaagac
     5041 aaacagaaag cgactggggg aaacgctgtg tggacaagtt tgacattatt gggattattg
     5101 gagaaggaac ctatggccaa gtatataaag ccaaggacaa agacacagga gaactagtgg
     5161 ctctgaagaa ggtgagacta gacaatgaga aagagggctt cccaatcaca gccattcgtg
     5221 aaatcaaaat ccttcgtcag ttaatccacc gaagtgttgt taacatgaag gaaattgtca
     5281 cagataaaca agatgcactg gatttcaaga aggacaaagg tgccttttac cttgtatttg
     5341 agtatatgga ccatgactta atgggactgc tagaatctgg tttggtgcac ttttctgagg
     5401 accatatcaa gtcgttcatg aaacagctaa tggaaggatt ggaatactgt cacaaaaaga
     5461 atttcctgca tcgggatatt aagtgttcta acattttgct gaataacagt gggcaaatca
     5521 aactagcaga ttttggactt gctcggctct ataactctga agagagtcgc ccttacacaa
     5581 acaaagtcat tactttgtgg taccgacctc cagaactact gctaggagag gaacgttaca
     5641 caccagccat agatgtttgg agctgtggat gtattcttgg ggaactattc acaaagaagc
     5701 ctatttttca agccaatctg gaactggctc agctagaact gatcagccga ctttgtggta
     5761 gcccttgtcc agctgtgtgg cctgatgtta tcaaactgcc ctacttcaac accatgaaac
     5821 cgaagaagca atatcgaagg cgtctacgag aagaattctc tttcattcct tctgcagcac
     5881 ttgatttatt ggaccacatg ctgacactag atcctagtaa gcggtgcaca gctgaacaga
     5941 ccctacagag cgacttcctt aaagatgtcg aactcagcaa aatggctcct ccagacctcc
     6001 cccactggca ggattgccat gagttgtgga gtaagaaacg gcgacgtcag cgacaaagtg
     6061 gtgttgtagt cgaagagcca cctccatcca aaacttctcg aaaagaaact acctcaggga
     6121 caagtactga gcctgtgaag aacagcagcc cagcaccacc tcagcctgct cctggcaagg
     6181 tggagtctgg ggctggggat gcaataggcc ttgctgacat cacacaacag ctgaatcaaa
     6241 gtgaattggc agtgttatta aacctgctgc agagccaaac cgacctgagc atccctcaaa
     6301 tggcacagct gcttaacatc cactccaacc cagagatgca gcagcagctg gaagccctga
     6361 accaatccat cagtgccctg acggaagcta cttcccagca gcaggactca gagaccatgg
     6421 ccccagagga gtctttgaag gaagcaccct ctgccccagt gatcctgcct tcagcagaac
     6481 agatgaccct tgaagcttca agcacaccag ctgacatgca gaatatattg gcagttctct
     6541 tgagtcagct gatgaaaacc caagagccag caggcagtct ggaggaaaac aacagtgaca
     6601 agaacagtgg gccacagggg ccccgaagaa ctcccacaat gccacaggag gaggcagcag
     6661 agaagaggcc ccctgagccc cccggacctc caccgccgcc acctccaccc cctctggttg
     6721 aaggcgatct ttccagcgcc ccccaggagt tgaacccagc cgtgacagcc gccttgctgc
     6781 aacttttatc ccagcctgaa gcagagcctc ctggccacct gccacatgag caccaggcct
     6841 tgagaccaat ggagtactcc acccgacccc gtccaaacag gacttatgga aacactgatg
     6901 ggcctgaaac agggttcagt gccattgaca ctgatgaacg aaactctggt ccagccttga
     6961 cagaatcctt ggtccagacc ctggtgaaga acaggacctt ctcaggctct ctgagccacc
     7021 ttggggagtc cagcagttac cagggcacag ggtcagtgca gtttccaggg gaccaggacc
     7081 tccgttttgc cagggtcccc ttagcgttac acccggtggt cgggcaacca ttcctgaagg
     7141 ctgagggaag cagcaattct gtggtacatg cagagaccaa attgcaaaac tatggggagc
     7201 tggggccagg aaccactggg gccagcagct caggagcagg ccttcactgg gggggcccaa
     7261 ctcagtcttc tgcttatgga aaactctatc gggggcctac aagagtccca ccaagagggg
     7321 gaagagggag aggagttcct tactcaggct cgagcggcaa cccagctttc ttgtacaaag
     7381 tggttgatta agatccacta gtaacggccg ccagtgtgct ggaattaatt cgctgtctgc
     7441 gagggccagc tgttggggtg agtactccct ctcaaaagcg ggcatgactt ctgcgctaag
     7501 attgtcagtt tccaaaaacg aggaggattt gatattcacc tggcccgcgg tgatgccttt
     7561 gagggtggcc gcgtccatct ggtcagaaaa gacaatcttt ttgttgtcaa gcttgaggtg
     7621 tggcaggctt gagatctggc catacacttg agtgacaatg acatccactt tgcctttctc
     7681 tccacaggtg tccactccca ggtccaactg caggtcgagc atgcatctag ggcggccaat
     7741 tccgcccctc tccccccccc ccctctccct cccccccccc taacgttact ggccgaagcc
     7801 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     7861 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     7921 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     7981 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     8041 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     8101 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     8161 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     8221 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     8281 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagcttgcc
     8341 acaacccaca aggagacgac cttccatgac cgagtacaag cccacggtgc gcctcgccac
     8401 ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     8461 cacgcgccac accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     8521 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     8581 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     8641 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     8701 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     8761 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     8821 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     8881 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     8941 catgacccgc aagcccggtg cctgatatcg atagatccta atcaacctct ggattacaaa
     9001 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     9061 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     9121 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     9181 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     9241 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     9301 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     9361 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     9421 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     9481 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     9541 cggatctccc tttgggccgc ctccccgcct gagatccttt aagaccaatg acttacaagg
     9601 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     9661 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     9721 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     9781 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     9841 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     9901 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggccc gggttaatta
     9961 aggaaagggc tagatcattc ttgaagacga aagggcctcg tgatacgcct atttttatag
    10021 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
    10081 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
    10141 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
    10201 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
    10261 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
    10321 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
    10381 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
    10441 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
    10501 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
    10561 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
    10621 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
    10681 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
    10741 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
    10801 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
    10861 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
    10921 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
    10981 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
    11041 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
    11101 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
    11161 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
    11221 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
    11281 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
    11341 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
    11401 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
    11461 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
    11521 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
    11581 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
    11641 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
    11701 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
    11761 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
    11821 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
    11881 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
    11941 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
    12001 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gcaagctcat ggctgactaa
    12061 ttttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
    12121 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt
    12181 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
    12241 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
    12301 ataacaattt cacacaggaa acagctatga catgattacg aatttcacaa ataaagcatt
    12361 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    12421 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
    12481 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
    12541 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag t
//
