LOCUS       sequence-267220-       11242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Booster-PKA for the generation of mice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11242)
  AUTHORS   Watabe T, Terai K, Sumiyama K, Matsuda M
  TITLE     Booster, a Red-Shifted Genetically Encoded Forster Resonance Energy 
            Transfer (FRET) Biosensor Compatible with Cyan Fluorescent 
            Protein/Yellow Fluorescent Protein-Based FRET Biosensors and Blue 
            Light-Responsive Optogenetic Tools.
  JOURNAL   ACS Sens. 2020 Feb 26. doi: 10.1021/acssensors.9b01941.
  PUBMED    32101394
REFERENCE   2  (bases 1 to 11242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Sens. 
            2020 Feb 26. doi: 10.1021/acssensors.9b01941."
COMMENT     Sequence Label: pT2ADW-lox-mCherryNLS-pA-lox-4493NES
FEATURES             Location/Qualifiers
     source          1..11242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        20..124
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(125..193,194..985)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(343..362)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1156..1744
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1645..1664
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1898..1915
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2032..2053
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2068..2073,2074..2091,2092..2098)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2106..2122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2111..2133
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2130..2146
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2130..2146
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2165..2185
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2167..2185
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(2242..2253,2254..2441)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     enhancer        3819..4198
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4200..4477
                     /label=chicken beta-actin promoter
     intron          4479..5496
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5420..5442
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     5504..5523
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(5553..5586)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5595..6299
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELY"
     primer_bind     complement(5740..5758)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6008..6027
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             6306..6326
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6339..6358)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6404..6459
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6458..6477)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    complement(6812..6845)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      6857..6866
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6863..7558
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     CDS             7994..8341
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             8090..8437
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             8186..8533
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             8282..8629
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             8378..8725
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             8732..9385
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQFKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             9431..9457
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     misc_feature    9481..10069
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9534..9554)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9952..9963)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    10134..10268
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10158..10177)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10212..10231
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(10390..10552,10553..10564)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(10594..10613)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10595..10613)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10623..10640)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10623..10639)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10632..10654)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10781..11236
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10868..10887)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11078..11099
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
       61 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      121 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      181 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      241 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
      301 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
      361 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
      421 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      481 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
      541 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
      601 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
      661 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
      721 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
      781 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
      841 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
      901 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
      961 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     1021 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1081 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1141 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1201 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1261 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1321 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1381 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1441 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1501 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1561 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1621 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1681 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1741 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     1801 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     1861 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     1921 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     1981 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     2041 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     2101 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     2161 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctcggatctg ctgggcttgc
     2221 tgaaggtagg gggtcaagaa ccagaggtgt aaagtacttg agtaatttta cttgattact
     2281 gtacttaagt attatttttg gggattttta ctttacttga gtacaattaa aaatcaatac
     2341 ttttactttt acttaattac atttttttag aaaaaaaagt actttttact ccttacaatt
     2401 ttatttacag tcaaaaagta cttatttttt ggagatcact tgggccctct agatgcatgc
     2461 cggctggccc aggccatcgg cattccggag cccagggtcc agatttggtt tcagaatgag
     2521 aggtcacgcc agctgaggca gcaccggcgg gaatctcggc cctggcccgg gagacgcggc
     2581 ccgccagaag gccggcgaaa gcggaccgcc gtcaccggat cccagaccgc cctgctcctc
     2641 cgagcctttg agaaggatcg ctttccaggc atcgccgccc gggaggagct ggccagagag
     2701 acgggcctcc cggagtccag gattcagatc tggtttcaga atcgaagggc caggcacccg
     2761 ggacagggtg gcagggcgcc cgcgcaggca ggcggcctgt gcagcgcggc ccccggcggg
     2821 ggtcaccctg ctccctcgtg ggtcgccttc gcccacaccg gcgcgtgggg aacggggctt
     2881 cccgcacccc acgtgccctg cgcgcctggg gctctcccac agggggcttt cgtgagccag
     2941 gcagcgaggg ccgcccccgc gctgcagccc agccaggccg cgccggcaga gggggtctcc
     3001 caacctgccc cggcgcgcgg ggatttcgcc tacgccgccc cggctcctcc ggacggggcg
     3061 ctctcccacc ctcaggctcc tcgctggcct ccgcacccgg gcaaaagccg ggaggaccgg
     3121 gacccgcagc gcgacggcct gccgggcccc tgcgcggtgg cacagcctgg gcccgctcaa
     3181 gcggggccgc agggccaagg ggtgcttgcg ccacccacgt cccaggggag tccgtggtgg
     3241 ggctggggcc ggggtcccca ggtcgccggg gcggcgtggg aaccccaagc cggggcagct
     3301 ccacctcccc agcccgcgcc cccggacgcc tccgcctccg cgcggcaggg gcagatgcaa
     3361 ggcatcccgg cgccctccca ggcgctccag gagccggcgc cctggtctgc actcccctgc
     3421 ggcctgctgc tggatgagct cctggcgagc ccggagtttc tgcagcaggc gcaacctctc
     3481 ctagaaacgg aggccccggg ggagctggag gcctcggaag aggccgcctc gctggaagca
     3541 cccctcagcg aggaagaata ccgggctctg ctggaggagc tttaggacgc ggggttggga
     3601 cggggtcggg tggttcgggg cagggcggtg gcctctcttt cgcggggaac acctggctgg
     3661 ctacggaggg gcgtgtctcc gccccgcccc ctccaccggg ctgaccggcc tgggattcct
     3721 gccttctagg tctaggcccg gtgagagact ccacaccgcg gagaactgcc attctttcct
     3781 gggcatcccg gggatcccag agccggccca gcatgctcga cattgattat tgactagtta
     3841 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     3901 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     3961 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     4021 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     4081 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     4141 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     4201 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat
     4261 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggg
     4321 gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc
     4381 ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg
     4441 gcggcggccc tataaaaagc gaagcgcgcg gcgggcgggg agtcgctgcg acgctgcctt
     4501 cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
     4561 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
     4621 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag
     4681 ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
     4741 gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt
     4801 tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg
     4861 gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg
     4921 ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag
     4981 cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg
     5041 ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag
     5101 ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc
     5161 cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa
     5221 atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg
     5281 aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc
     5341 cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg
     5401 gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct
     5461 gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
     5521 gtgctgtctc atcattttgg caaagaatta ccataacttc gtatagcata cattatacga
     5581 agttattgaa gcttatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
     5641 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     5701 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     5761 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
     5821 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
     5881 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
     5941 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     6001 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
     6061 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
     6121 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     6181 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
     6241 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtact
     6301 ctagacctaa gaagaagcgt aaggtgtaag tcgacggatc tttttccctc tgccaaaaat
     6361 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt
     6421 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
     6481 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgccata
     6541 tgctggctgc catgaacaaa ggtggctata aagaggtcat cagtatatga aacagccccc
     6601 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt ttttttatat
     6661 tttgtttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc
     6721 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatgaag
     6781 atccctcgac ctgcagccca agcttgctag cataacttcg tatagcatac attatacgaa
     6841 gttattggaa ttcgccgcca ccatggtgag cgagctgatt aaggagaaca tgcacatgaa
     6901 gctgtacatg gagggcaccg tgaacaacca ccacttcaag tgcacatccg agggcgaagg
     6961 caagccctac gagggcaccc agaccatgag aatcaaggcg gtcgagggcg gccctctccc
     7021 cttcgccttc gacatcctgg ctaccagctt catgtacggc agcaaaacct tcatcaacca
     7081 cacccagggc atccccgact tctttaagca gtccttcccc gagggcttca catgggagag
     7141 agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccagga
     7201 cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcccatcca acggccctgt
     7261 gatgcagaag aaaacactcg gctgggaggc ctccaccgag accctgtacc ccgctgacgg
     7321 cggcctggaa ggcagagccg acatggccct gaagctcgtg ggcgggggcc acctgatctg
     7381 caacttgaag accacataca gatccaagaa acccgctaag aacctcaaga tgcccggcgt
     7441 ctactatgtg gacagaagac tggaaagaat caaggaggcc gacaaagaga cctacgtcga
     7501 gcagcacgag gtggctgtgg ccagatactg cgacctccct agcaaactgg ggcacagact
     7561 cgagaagttt tctcaagaac agatcggcga aaacattgtg tgcagggtca tttgtaccac
     7621 gggtcaaatt cccatccgag atttgtcagc tgatatttca caagtgctta aggaaaaacg
     7681 atccataaag aaagtttgga catttggtag aaacccagcc tgtgactatc atttaggaaa
     7741 catttcaaga ctgtcaaata agcatttcca aatactacta ggagaagacg gtaacctttt
     7801 attgaatgac atttccacta atgggacctg gttaaatggg caaaaagtcg agaagaacag
     7861 caatcagtta ctgtcccaag gtgatgaaat aaccgttggt gtaggcgtgg aatcagatat
     7921 tttatctctg gtcattttca taaacgacaa atttaagcag tgcctggagc agaacaaagt
     7981 tgatcgcggt accagtgctg gtggtagtgc tggtggtagt gctggtggta gtgctggtgg
     8041 tagtgctggt ggttccggca gtgctggtgg tagtgctggt ggtagtacca gtgctggtgg
     8101 tagtgctggt ggtagtgctg gtggtagtgc tggtggtagt gctggtggtt ccggcagtgc
     8161 tggtggtagt gctggtggta gtaccagtgc tggtggtagt gctggtggta gtgctggtgg
     8221 tagtgctggt ggtagtgctg gtggttccgg cagtgctggt ggtagtgctg gtggtagtac
     8281 cagtgctggt ggtagtgctg gtggtagtgc tggtggtagt gctggtggta gtgctggtgg
     8341 ttccggcagt gctggtggta gtgctggtgg tagtaccagt gctggtggta gtgctggtgg
     8401 tagtgctggt ggtagtgctg gtggtagtgc tggtggttcc ggcagtgctg gtggtagtgc
     8461 tggtggtagt accagtgctg gtggtagtgc tggtggtagt gctggtggta gtgctggtgg
     8521 tagtgctggt ggttccggca gtgctggtgg tagtgctggt ggtagtacca gtgctggtgg
     8581 tagtgctggt ggtagtgctg gtggtagtgc tggtggtagt gctggtggtt ccggcagtgc
     8641 tggtggtagt gctggtggta gtaccagtgc tggtggtagt gctggtggta gtgctggtgg
     8701 tagtgctggt ggtagtgctg gtggttccgg aatggtgagt gtgattaaac cagagatgaa
     8761 gatgaggtac tacatggacg gctccgtcaa tgggcatgag ttcacaattg aaggtgaagg
     8821 cacaggcaga ccttacgagg gacatcaaga gatgacacta cgcgtcacaa tggccgaggg
     8881 cgggccaatg cctttcgcgt ttgacttagt gtcacacgtg ttctgttacg gccacagagt
     8941 atttactaaa tatccagaag agataccaga ctatttcaaa caagcatttc ctgaaggcct
     9001 gtcatgggaa aggtcgttgg agttcgaaga tggtgggtcc gcttcagtca gtgcgcatat
     9061 aagccttaga ggaaacacct tctaccacaa atccaaattt actggggtta actttcctgc
     9121 cgatggtcct atcatgcaaa accaaagtgt tgattgggag ccatcaaccg agaaaattac
     9181 tgccagcgac ggagttctga agggtgatgt tacgatgtac ctaaaacttg aaggaggcgg
     9241 caatcacaaa tgccaattca agactactta caaggcggca aaagagattc ttgaaatgcc
     9301 aggagaccat tacatcggcc atcgcctcgt caggaaaacc gaaggcaaca ttactgagca
     9361 ggtagaagat gcagtagctc attccgcggc cgcattgagg cgcgcgacgc tggttgactc
     9421 tagactgcag ctgcctcctc tggaacgcct gactctggat taagtcgacg gatcctcgat
     9481 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     9541 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     9601 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     9661 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     9721 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     9781 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     9841 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     9901 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     9961 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
    10021 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tctcgacaaa
    10081 ggatccaatg gaggccgcca ccgcggtgga gctcgaatta attcatcgat gatgatccag
    10141 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
    10201 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
    10261 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
    10321 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctag
    10381 atcagatctt tcttgctttt acttttactt ccttaatact caagtacaat tttaatggag
    10441 tactttttta cttttactca agtaagattc tagccagata cttttacttt taattgagta
    10501 aaattttccc taagtacttg tactttcact tgagtaaaat ttttgagtac tttttacacc
    10561 tctgtcaaga accatatgcc ggtacccaat tcgccctata gtgagtcgta ttacgcgcgc
    10621 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
    10681 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
    10741 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
    10801 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
    10861 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
    10921 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
    10981 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
    11041 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
    11101 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
    11161 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
    11221 ttaacgctta caatttaggt gg
//
