LOCUS       sequence-267242-        6211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  10xUAS expression vector with a vermilion marker and a phiC31 attB 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Kondo S, Takahashi T, Yamagata N, Imanishi Y, Katow H, Hiramatsu S, 
            Lynn K, Abe A, Kumaraswamy A, Tanimoto H
  TITLE     Neurochemical Organization of the Drosophila Brain Visualized by 
            Endogenously Tagged Neurotransmitter Receptors.
  JOURNAL   Cell Rep. 2020 Jan 7;30(1):284-297.e5. doi: 
            10.1016/j.celrep.2019.12.018.
  PUBMED    31914394
REFERENCE   2  (bases 1 to 6211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.12"; journalName: "Cell Rep"; date: 
            "2020-01-7- 7"; volume: "30"; issue: "1"; pages: "284-297.e5"
COMMENT     Sequence Label: pBFv-UAS3
FEATURES             Location/Qualifiers
     source          1..6211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(179..296,373..466,558..1164,1219..1352,
                     1413..1573,1647..1672))
                     /codon_start=1
                     /product="tryptophan oxygenase"
                     /label=vermilion
                     /note="selectable marker, required to synthesize the brown 
                     eye pigment in Drosophila"
                     /translation="MSCPYAGNGNDHDDSAVPLTTEVGKIYGEYLMLDKLLDAQCMLSE
                     EDKRPVHDEHLFIITHQAYELWFKQIIFEFDSIRDMLDAEVIDETKTLEIVKRLNRVVL
                     ILKLLVDQVPILETMTPLDFMDFRKYLAPASGFQSLQFRLIENKLGVLTEQRVRYNQKY
                     SDVFSDEEARNSIRNSEKDPSLLELVQRWLERTPGLEESGFNFWAKFQESVDRFLEAQV
                     QSAMEEPVEKAKNYRLMDIEKRREVYRSIFDPAVHDALVRRGDRRFSHRALQGAIMITF
                     YRDEPRFSQPHQLLTLLMDIDSLITKWRYNHVIMVQRMIGSQQLGTGGSSGYQYLRSTL
                     SDRYKVFLDLFNLSTFLIPREAIPPLDETIRKKLINKSV"
     protein_bind    1944..2038
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    2070..2164
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        2193..2431
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     2378..2399
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     primer_bind     complement(2484..2500)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2484..2500)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3081..3101)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3081..3099)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3120..3136)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3120..3136)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3133..3155)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3144..3160
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3168..3174,3175..3192,3193..3198))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3213..3234
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3351..3368)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3522..4110)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3602..3621)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4281..5072,5073..5141))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4904..4923
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5142..5246)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5272..5727)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5409..5430)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5621..5640
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5854..5876
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5868..5885
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5869..5885
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5895..5914
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5895..5913
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6121..6190
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 aagcttggat ttattttgtt atgttatatg tattatatgt cagacataaa gaaaaggaac
       61 acatcaaatg tgataacaaa gactaaacaa gtaattttat tacaccaaaa cgacaaaaca
      121 gtaggcagaa caaacaacgc atagccaaac attgacgaat tggataccct gccgattgtc
      181 agacactttt gttgatcagt ttcttgcgaa tggtctcgtc cagcggtgga atcgcctcgc
      241 ggggaatcag aaaagtggac agattgaaca gatccagaaa caccttgtac cgatcactga
      301 aaccaaaaaa aaacaaaggg agaacagttt gagttcattg atccccgata taatcacatc
      361 tgcgatgatc acctgagagt ggagcgcaga tattgatatc cagacgagcc accagtgccc
      421 aactgttggg atccaatcat gcgttgcacc atgatcacgt gattgtctgc ggcgggaata
      481 gaaagtattt ggttaggaaa accagtctta aacataagat atatttataa aagagtatca
      541 aagaatgcaa tacttacatc tccacttggt tattaacgag tcgatgtcca tgagcagggt
      601 gagcaactgg tgtggttggc tgaacctggg ttcatcccta tagaaggtga tcatgatggc
      661 tccctgaagg gcacgatggc taaaccggcg atccccacga cgcaccagtg catcgtgcac
      721 tgccggatca aagatggagc gatacacctc gcgtcgcttc tcaatgtcca tgaggcggta
      781 gtttttcgcc ttctccacgg gctcctccat ggcgctctgt acctgcgcct ccaggaatcg
      841 atcgacgctc tcctgaaact tggcccagaa gttgaagcca ctctcctcca gtccgggcgt
      901 cctctccagc catcgctgca ctagctccag tagcgaggga tctttctccg agttgcgaat
      961 cgagttccgc gcctcctcgt cgctaaagac atccgagtac ttctggttgt atctcacccg
     1021 ctgctctgtc agaactccca gcttgttctc gatcaaacgg aactgcagcg actgaaaacc
     1081 agatgcgggt gccaggtact tgcggaagtc catgaagtct agcggggtca tggtctccag
     1141 aatgggcact tggtccacca ggagctgtac aaaggaagtt ataaacggat tttggtaaga
     1201 gattcagaaa gcactcactt ttagaatcag aaccactcgg ttcagtcgct tgacaatctc
     1261 cagcgtcttg gtttcatcga tgacctctgc atccaacatg tctcgtatgg agtcgaactc
     1321 aaagatgatc tgcttgaacc aaagctcgta ggctgtggcg aaggtactta aatgccattg
     1381 agtgttgtca tcaaagttgt aaacctactc accctggtgc gtgatgatga acagatgctc
     1441 atcgtgcacg ggtcgcttgt cctcctcgga cagcatacac tgggcatcca gcagtttgtc
     1501 cagcatcaga tactctccat agattttgcc cacttccgtg gttaatggca ccgccgaatc
     1561 atcgtgatcg tttctgtatg ggtttgaatt gaatcgcaga actgaagatc gattggcatt
     1621 cctggacagc acgtgctggt gctcacccgt ttcctgcata gggacagctc atggtgcaca
     1681 gctcagatca gatcgtgact cctcgagcgg cggatgctgg cgaactgatc tccgccagcg
     1741 gaccggagat gagaccccag cgaaccgata acagagcgag agagctccag ttccgactga
     1801 ttgcacagtc ggtgatctgg gcgatgggca ctgccagata ggctgggaat tatcaatcac
     1861 ttgaggtgaa agtgcggcgc acacaaatcc aagcttgata tcgaattccc tgcagggcca
     1921 accaacaacc ttaagctgca ggtcggagta ctgtcctccg agcggagtac tgtcctccga
     1981 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
     2041 gagactctag cgagcacgcg tctgcaggtc ggagtactgt cctccgagcg gagtactgtc
     2101 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
     2161 cgagcggaga ctctagcgag ccatatggag cgccggagta taaatagagg cgcttcgtct
     2221 acggagcgac aattcaattc aaacaagcaa agtgaacacg tcgctaagcg aaagctaagc
     2281 aaataaacaa gcgcagctga acaagctaaa caatctgcag taaagtgcaa gttaaagtga
     2341 atcaattaaa agtaaccagc aaccaagtaa atcaactgca actactgaaa tctgccaaga
     2401 agtaattatt gaatacaaga agagaactct gaatagggaa ttggggcgcg ccgctagcag
     2461 atctgaattc ctgcagcccg ggggatccac tagttctaga gcggccgcgc gtcacgccac
     2521 ttcaacgctc gatgggagcg tcattggtgg gcggggtaac cgtcgaaatc agtgtttacg
     2581 cttccaatcg caacaaaaaa ttcactgcaa cactgaaaag catacgaaaa cgatgaagat
     2641 tgtacgagaa accataaagt attttatcca caaagacacg tatagcagaa aagccaagtt
     2701 aactcggcga taagttgtgt acacaagaat aaaatcggcc agattcagtg ttgtcagaaa
     2761 taagaaaacc ccactatgtt tttctttgcc ttttctttct cccagcgatc attcatttcg
     2821 tggtgaaaga acggggtcat tgcacggagt ttcgactgcg ggaaagcaga gctgccgttc
     2881 acttcgtcta taattagcgc tttctatttt ccccgattcg ggccgctgct gcgcttttcc
     2941 gcctgctgtt tgtggcaagt gtagcagcag gctgtgcacg cagtgtgtag ggataacagg
     3001 gtaatgacgt cgcatgcact tggctttcca ccgttggtat cgattctctg ggacgatgag
     3061 tcgagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg
     3121 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     3181 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     3241 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     3301 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     3361 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     3421 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     3481 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     3541 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     3601 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     3661 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     3721 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3781 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3841 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3901 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3961 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     4021 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     4081 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     4141 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     4201 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     4261 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     4321 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     4381 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     4441 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     4501 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     4561 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     4621 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     4681 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     4741 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     4801 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     4861 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     4921 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     4981 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     5041 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     5101 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     5161 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     5221 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
     5281 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     5341 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     5401 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     5461 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     5521 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     5581 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     5641 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     5701 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg
     5761 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     5821 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     5881 ccagtgagcg cgcgtaatac gactcactat agggcgaatt gggtacctcg acatgcccgc
     5941 cgtgaccgtc gagaacccgc tgacgctgcc ccgcgtatcc gcacccgccg acgccgtcgc
     6001 acgtcccgtg ctcaccgtga ccaccgcgcc cagcggtttc gagggcgagg gcttcccggt
     6061 gcgccgcgcg ttcgccggga tcaactaccg ccacctcgac ccgttcatca tgatggacca
     6121 gatgggtgag gtggagtacg cgcccgggga gcccaagggc acgccctggc acccgcaccg
     6181 cggcttcgag accgtgacct acatcgtcga c
//
