LOCUS       sequence-26726-e         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral expression of humanized ChR2 with H134R mutation 
            fused to EYFP driven by EF1a promoter for optogenetic activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Zhang F, Wang LP, Brauner M, Liewald JF, Kay K, Watzke N, Wood PG, 
            Bamberg E, Nagel G, Gottschalk A, Deisseroth K
  TITLE     Multimodal fast optical interrogation of neural circuitry.
  JOURNAL   Nature. 2007 Apr 5. 446(7136):633-9.
  PUBMED    17410168
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Apr 5. 446(7136):633-9."
COMMENT     Sequence Label: pLenti-EF1a-hChR2(H134R)-EYFP-WPRE
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cctngctcac catggtggcg gccgctggca cggctccggc ctcggcttcg tcttcgacga
       61 gagtctcgac ctcgatctcc gttccgccga tgttcagttt ggtggttttg cggatatctc
      121 cgtgaatcaa tatgtgctcg tggatcagga cgcgcaggta gtgtcccaac aacccccaac
      181 aatttttact catcagatca ataatcgtgt gacctacggt ggagccatag acgctcagga
      241 cgccaaaacc ttcgggcccc aaaatgaaga gaattgggaa cataccccag ctcacgaaaa
      301 acagccatgc catgccggtc acgacctggc ggcaccgacc ctttggcaca gtatgataac
      361 cctcgatata tgctttggcg gcgtgaaaaa atgtgttcgc gccatagcac aatccaagac
      421 aaaagaagat gactttaaca tagccggttg ccatggcgct ggtagccccc cacacgatag
      481 tcccgatgtc tgagacaagg agtcccatgg ttctcctgct gtagtcgttg ctcaggccgg
      541 tgaggttgct caggcggata aggatgacag gacaagtgag cagccactct gcatagcgca
      601 gccactgcac ccggtgtcct gtggcaaggt agagcataga gggattctta aactcaaaaa
      661 agaactcgag aatcacctta accatttcaa tggcgcacac atagatctcc tcccagccgc
      721 atgtagattt ccaggtttgg taggcataga acatcagcag caa
//
