LOCUS       sequence-267266-       10043 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid contains basal promoter driving firefly 
            Luciferase 2 and Renilla Luciferase driven by TK promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10043)
  AUTHORS   Sissaoui S, Yu J, Yan A, Li R, Zhu LJ, Kucukural A, Yukselen O, 
            Lawson ND
  TITLE     Genomic Characterization of Endothelial Enhancers Reveals a 
            Multifunctional Role for NR2F2 in Regulation of Arteriovenous Gene 
            Expression.
  JOURNAL   Circ Res. 2020 Feb 17. doi: 10.1161/CIRCRESAHA.119.316075.
  PUBMED    32065070
REFERENCE   2  (bases 1 to 10043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Circ Res. 
            2020 Feb 17. doi: 10.1161/CIRCRESAHA.119.316075."
COMMENT     Sequence Label: pLenti-basP:fLuc-TK:rLuc
FEATURES             Location/Qualifiers
     source          1..10043
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    47..67
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        69..87
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    122..146
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        153..184
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      211..220
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             217..1869
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     protein_bind    complement(1883..1907)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        1908..2659
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     intron          2729..2861
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     2844..2863
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        2906..2924
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2934..3869
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     protein_bind    complement(3917..3937)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    3952..4540
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4005..4025)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4423..4434)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4627..4860
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4932..5053
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4969..4988)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5023..5042
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5072..5207
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(5240..5258)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5268..5285)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5268..5284)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5277..5299)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5429..5884
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5516..5535)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5726..5747
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5913..6017
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6018..6086,6087..6878)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6236..6255)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7049..7637
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7538..7557
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7791..7808
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7925..7946
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7961..7966,7967..7984,7985..7991)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7999..8015
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8004..8026
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8023..8039
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8023..8039
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8058..8078
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8060..8078
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             8334..8514
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8558..8683
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9176..9409
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9594..9638
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9905..10022
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctagcccggg ctcgagcagc tgaagcttgc atgcctgcag ggctatcaac tttgtataga
       61 aaagttggta atacgactca ctatagggcg aattgggtac ctcgagttaa cggatccccg
      121 ggcaagtttg tacaaaaaag caggctagac actagagggt atataatgga agctcgactt
      181 ccagcttggc aatccggtac tgttggtaaa gccaccatgg aagatgccaa aaacattaag
      241 aagggcccag cgccattcta cccactcgaa gacgggaccg ccggcgagca gctgcacaaa
      301 gccatgaagc gctacgccct ggtgcccggc accatcgcct ttaccgacgc acatatcgag
      361 gtggacatta cctacgccga gtacttcgag atgagcgttc ggctggcaga agctatgaag
      421 cgctatgggc tgaatacaaa ccatcggatc gtggtgtgca gcgagaatag cttgcagttc
      481 ttcatgcccg tgttgggtgc cctgttcatc ggtgtggctg tggccccagc taacgacatc
      541 tacaacgagc gcgagctgct gaacagcatg ggcatcagcc agcccaccgt cgtattcgtg
      601 agcaagaaag ggctgcaaaa gatcctcaac gtgcaaaaga agctaccgat catacaaaag
      661 atcatcatca tggatagcaa gaccgactac cagggcttcc aaagcatgta caccttcgtg
      721 acttcccatt tgccacccgg cttcaacgag tacgacttcg tgcccgagag cttcgaccgg
      781 gacaaaacca tcgccctgat catgaacagt agtggcagta ccggattgcc caagggcgta
      841 gccctaccgc accgcaccgc ttgtgtccga ttcagtcatg cccgcgaccc catcttcggc
      901 aaccagatca tccccgacac cgctatcctc agcgtggtgc catttcacca cggcttcggc
      961 atgttcacca cgctgggcta cttgatctgc ggctttcggg tcgtgctcat gtaccgcttc
     1021 gaggaggagc tattcttgcg cagcttgcaa gactataaga ttcaatctgc cctgctggtg
     1081 cccacactat ttagcttctt cgctaagagc actctcatcg acaagtacga cctaagcaac
     1141 ttgcacgaga tcgccagcgg cggggcgccg ctcagcaagg aggtaggtga ggccgtggcc
     1201 aaacgcttcc acctaccagg catccgccag ggctacggcc tgacagaaac aaccagcgcc
     1261 attctgatca cccccgaagg ggacgacaag cctggcgcag taggcaaggt ggtgcccttc
     1321 ttcgaggcta aggtggtgga cttggacacc ggtaagacac tgggtgtgaa ccagcgcggc
     1381 gagctgtgcg tccgtggccc catgatcatg agcggctacg ttaacaaccc cgaggctaca
     1441 aacgctctca tcgacaagga cggctggctg cacagcggcg acatcgccta ctgggacgag
     1501 gacgagcact tcttcatcgt ggaccggctg aagagcctga tcaaatacaa gggctaccag
     1561 gtagccccag ccgaactgga gagcatcctg ctgcaacacc ccaacatctt cgacgccggg
     1621 gtcgccggcc tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa
     1681 cacggtaaaa ccatgaccga gaaggagatc gtggactatg tggccagcca ggttacaacc
     1741 gccaagaagc tgcgcggtgg tgttgtgttc gtggacgagg tgcctaaagg actgaccggc
     1801 aagttggacg cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc
     1861 gccgtgtaat aattctagag tcacccagct ttcttgtaca aagtggaaat gagtcttcgg
     1921 acctcgcggg ggccgcttaa gcggtggtta gggtttgtct gacgcggggg gagggggaag
     1981 gaacgaaaca ctctcattcg gaggcggctc ggggtttggt cttggtggcc acgggcacgc
     2041 agaagagcgc cgcgatcctc ttaagcaccc ccccgccctc cgtggaggcg ggggtttggt
     2101 cggcgggtgg taactggcgg gccgctgact cgggcgggtc gcgcgcccca gagtgtgacc
     2161 ttttcggtct gctcgcagac ccccgggcgg cgccgccgcg gcggcgacgg gctcgctggg
     2221 tcctaggctc catggggacc gtatacgtgg acaggctctg gagcatccgc acgactgcgg
     2281 tgatattacc ggagaccttc tgcgggacga gccgggtcac gcggctgacg cggagcgtcc
     2341 gttgggcgac aaacaccagg acggggcaca ggtacactat cttgtcaccc ggaggcgcga
     2401 gggactgcag gagcttcagg gagtggcgca gctgcttcat ccccgtggcc cgttgctcgc
     2461 gtttgctggc ggtgtccccg gaagaaatat atttgcatgt ctttagttct atgatgacac
     2521 aaaccccgcc cagcgtcttg tcattggcga attcgaacac gcagatgcag tcggggcggc
     2581 gcggtcccag gtccacttcg catattaagg tgacgcgtgt ggcctcgaac accgagcgac
     2641 cctgcagcga cccgcttaaa agcttgattc ttctgacaca acagtctcga acttaagctg
     2701 cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag gttacaagac aggtttaagg
     2761 agaccaatag aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     2821 tattggtctt actgacatcc actttgcctt tctctccaca ggtgtccact cccagttcaa
     2881 ttacagctct taaggctaga gtacttaata cgactcacta taggctagcc accatgactt
     2941 cgaaagttta tgatccagaa caaaggaaac ggatgataac tggtccgcag tggtgggcca
     3001 gatgtaaaca aatgaatgtt cttgattcat ttattaatta ttatgattca gaaaaacatg
     3061 cagaaaatgc tgttattttt ttacatggta acgcggcctc ttcttattta tggcgacatg
     3121 ttgtgccaca tattgagcca gtagcgcggt gtattatacc agaccttatt ggtatgggca
     3181 aatcaggcaa atctggtaat ggttcttata ggttacttga tcattacaaa tatcttactg
     3241 catggtttga acttcttaat ttaccaaaga agatcatttt tgtcggccat gattggggtg
     3301 cttgtttggc atttcattat agctatgagc atcaagataa gatcaaagca atagttcacg
     3361 ctgaaagtgt agtagatgtg attgaatcat gggatgaatg gcctgatatt gaagaagata
     3421 ttgcgttgat caaatctgaa gaaggagaaa aaatggtttt ggagaataac ttcttcgtgg
     3481 aaaccatgtt gccatcaaaa atcatgagaa agttagaacc agaagaattt gcagcatatc
     3541 ttgaaccatt caaagagaaa ggtgaagttc gtcgtccaac attatcatgg cctcgtgaaa
     3601 tcccgttagt aaaaggtggt aaacctgacg ttgtacaaat tgttaggaat tataatgctt
     3661 atctacgtgc aagtgatgat ttaccaaaaa tgtttattga atcggaccca ggattctttt
     3721 ccaatgctat tgttgaaggt gccaagaagt ttcctaatac tgaatttgtc aaagtaaaag
     3781 gtcttcattt ttcgcaagaa gatgcacctg atgaaatggg aaaatatatc aaatcgttcg
     3841 ttgagcgagt tctcaaaaat gaacaataat tctagagcgg ccgcttcgag cagacatgat
     3901 aagatacatt gatgagcaac tttattatac atagttgata attcactcga caatcaacct
     3961 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     4021 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     4081 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     4141 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     4201 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     4261 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     4321 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     4381 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     4441 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     4501 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattcg agctcggtac
     4561 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     4621 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     4681 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     4741 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     4801 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     4861 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     4921 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     4981 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5041 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagttccgc
     5101 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     5161 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcgt
     5221 cgagacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     5281 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     5341 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     5401 ttgcgcagcc tgaatggcga atggcgcgac gcgccctgta gcggcgcatt aagcgcggcg
     5461 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     5521 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5581 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5641 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5701 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5761 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     5821 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     5881 atttcccagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5941 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     6001 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     6061 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     6121 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     6181 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     6241 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     6301 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     6361 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     6421 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     6481 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     6541 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     6601 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     6661 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6721 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6781 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6841 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     6901 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6961 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     7021 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     7081 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     7141 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     7201 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     7261 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     7321 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     7381 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     7441 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     7501 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     7561 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     7621 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     7681 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     7741 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     7801 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     7861 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     7921 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     7981 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     8041 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     8101 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     8161 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     8221 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
     8281 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
     8341 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     8401 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     8461 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     8521 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
     8581 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     8641 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     8701 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     8761 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     8821 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     8881 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     8941 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     9001 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     9061 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     9121 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     9181 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     9241 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     9301 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     9361 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     9421 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     9481 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     9541 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     9601 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     9661 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     9721 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     9781 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     9841 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt
     9901 taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     9961 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
    10021 ttatcgatca cgagactagc ctc
//
