LOCUS       sequence-267270-       10979 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid contains 161k modified human enhancer with with 
            NR2F2 binding sites mutated driving firefly Luciferase 2 and Renilla
            Luciferase driven by TK promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10979)
  AUTHORS   Sissaoui S, Yu J, Yan A, Li R, Zhu LJ, Kucukural A, Yukselen O, 
            Lawson ND
  TITLE     Genomic Characterization of Endothelial Enhancers Reveals a 
            Multifunctional Role for NR2F2 in Regulation of Arteriovenous Gene 
            Expression.
  JOURNAL   Circ Res. 2020 Feb 17. doi: 10.1161/CIRCRESAHA.119.316075.
  PUBMED    32065070
REFERENCE   2  (bases 1 to 10979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Circ Res. 
            2020 Feb 17. doi: 10.1161/CIRCRESAHA.119.316075."
COMMENT     Sequence Label: pLenti-161K enhancer-NR2F2-MUT-minP:fLuc-TK:rLuc
FEATURES             Location/Qualifiers
     source          1..10979
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    47..67
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1058..1082
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        1089..1120
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      1147..1156
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1153..2805
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     protein_bind    complement(2819..2843)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2844..3595
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     intron          3665..3797
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3780..3799
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        3842..3860
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3870..4805
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     protein_bind    complement(4853..4873)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    4888..5476
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4941..4961)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5359..5370)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5563..5796
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5868..5989
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5905..5924)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5959..5978
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6008..6143
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6176..6194)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6204..6221)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6204..6220)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6213..6235)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6365..6820
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6452..6471)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6662..6683
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6849..6953
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6954..7022,7023..7814)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7172..7191)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7985..8573
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8474..8493
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8727..8744
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8861..8882
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8897..8902,8903..8920,8921..8927)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8935..8951
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8940..8962
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8959..8975
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8959..8975
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8994..9014
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8996..9014
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             9270..9450
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9494..9619
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10112..10345
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10530..10574
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10841..10958
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctagcccggg ctcgagcagc tgaagcttgc atgcctgcag ggctatcaac tttgtataga
       61 aaagttgggc tgctgattca tatcccagtg aggtcatctg aattttgggg gacctcaaat
      121 agaaatcata tgggggaaag aatgagccta gctcaaacca gacaggctga gagcttatct
      181 tgccaggaca taggctcccc ggtgggtggg ggagatggcc attgctgcct ttctttcctt
      241 ccccactgaa tttaagcaga tgcagttagt cactgtgcaa aggagtatgt gcaaatctaa
      301 gtattcatgt ttatgtgtgg ttgtcaggtc taaatcagcc aggcagtaat ttctctggga
      361 gcccaatgta ggcactaaat tttgcactca accaccgaat ggtccacatg ccccttaatc
      421 tgtcttcctg caggagggct cccttggcac catcctgcct gaacttgggg ccagcctttc
      481 actggagccc cttcccgtga attatggcct acagcatggg ggctgaatag aaaaaaaaaa
      541 accctccaga aattcaactg gaagggctga ccggaattca acctaatgtt gagaatagca
      601 ggaaagcttc tattattcta cttcattcta tctgctatga ggcagtgcgg tcgcctggag
      661 tcgactgagc ttaactcatt tccctggatt tccgagtcac agccatggtg cctgctatag
      721 ttaacgatcg aactcctgag actcactaca cttcctgata ggacgtactc acgtaccgcc
      781 tagtaacatc atacgttcct ataggagtca taatatgacc tagacaagac ctaggagcca
      841 atcagaatgc aaatgattga tgggagtgct tttggttatc tactgtttgt cctgacgctt
      901 ggcgtggttc tttgtattat gattgtgtgg gcagtaggct taaaaataca tacaggcatt
      961 gctttccaca ttgttgaagg ttaatcctaa ggtgtggcaa agaaactata ttttatagac
     1021 tggaacctca tgggcaccag ctagtttttg ctcttcctca agtttgtaca aaaaagcagg
     1081 ctagacacta gagggtatat aatggaagct cgacttccag cttggcaatc cggtactgtt
     1141 ggtaaagcca ccatggaaga tgccaaaaac attaagaagg gcccagcgcc attctaccca
     1201 ctcgaagacg ggaccgccgg cgagcagctg cacaaagcca tgaagcgcta cgccctggtg
     1261 cccggcacca tcgcctttac cgacgcacat atcgaggtgg acattaccta cgccgagtac
     1321 ttcgagatga gcgttcggct ggcagaagct atgaagcgct atgggctgaa tacaaaccat
     1381 cggatcgtgg tgtgcagcga gaatagcttg cagttcttca tgcccgtgtt gggtgccctg
     1441 ttcatcggtg tggctgtggc cccagctaac gacatctaca acgagcgcga gctgctgaac
     1501 agcatgggca tcagccagcc caccgtcgta ttcgtgagca agaaagggct gcaaaagatc
     1561 ctcaacgtgc aaaagaagct accgatcata caaaagatca tcatcatgga tagcaagacc
     1621 gactaccagg gcttccaaag catgtacacc ttcgtgactt cccatttgcc acccggcttc
     1681 aacgagtacg acttcgtgcc cgagagcttc gaccgggaca aaaccatcgc cctgatcatg
     1741 aacagtagtg gcagtaccgg attgcccaag ggcgtagccc taccgcaccg caccgcttgt
     1801 gtccgattca gtcatgcccg cgaccccatc ttcggcaacc agatcatccc cgacaccgct
     1861 atcctcagcg tggtgccatt tcaccacggc ttcggcatgt tcaccacgct gggctacttg
     1921 atctgcggct ttcgggtcgt gctcatgtac cgcttcgagg aggagctatt cttgcgcagc
     1981 ttgcaagact ataagattca atctgccctg ctggtgccca cactatttag cttcttcgct
     2041 aagagcactc tcatcgacaa gtacgaccta agcaacttgc acgagatcgc cagcggcggg
     2101 gcgccgctca gcaaggaggt aggtgaggcc gtggccaaac gcttccacct accaggcatc
     2161 cgccagggct acggcctgac agaaacaacc agcgccattc tgatcacccc cgaaggggac
     2221 gacaagcctg gcgcagtagg caaggtggtg cccttcttcg aggctaaggt ggtggacttg
     2281 gacaccggta agacactggg tgtgaaccag cgcggcgagc tgtgcgtccg tggccccatg
     2341 atcatgagcg gctacgttaa caaccccgag gctacaaacg ctctcatcga caaggacggc
     2401 tggctgcaca gcggcgacat cgcctactgg gacgaggacg agcacttctt catcgtggac
     2461 cggctgaaga gcctgatcaa atacaagggc taccaggtag ccccagccga actggagagc
     2521 atcctgctgc aacaccccaa catcttcgac gccggggtcg ccggcctgcc cgacgacgat
     2581 gccggcgagc tgcccgccgc agtcgtcgtg ctggaacacg gtaaaaccat gaccgagaag
     2641 gagatcgtgg actatgtggc cagccaggtt acaaccgcca agaagctgcg cggtggtgtt
     2701 gtgttcgtgg acgaggtgcc taaaggactg accggcaagt tggacgcccg caagatccgc
     2761 gagattctca ttaaggccaa gaagggcggc aagatcgccg tgtaataatt ctagagtcac
     2821 ccagctttct tgtacaaagt ggaaatgagt cttcggacct cgcgggggcc gcttaagcgg
     2881 tggttagggt ttgtctgacg cggggggagg gggaaggaac gaaacactct cattcggagg
     2941 cggctcgggg tttggtcttg gtggccacgg gcacgcagaa gagcgccgcg atcctcttaa
     3001 gcaccccccc gccctccgtg gaggcggggg tttggtcggc gggtggtaac tggcgggccg
     3061 ctgactcggg cgggtcgcgc gccccagagt gtgacctttt cggtctgctc gcagaccccc
     3121 gggcggcgcc gccgcggcgg cgacgggctc gctgggtcct aggctccatg gggaccgtat
     3181 acgtggacag gctctggagc atccgcacga ctgcggtgat attaccggag accttctgcg
     3241 ggacgagccg ggtcacgcgg ctgacgcgga gcgtccgttg ggcgacaaac accaggacgg
     3301 ggcacaggta cactatcttg tcacccggag gcgcgaggga ctgcaggagc ttcagggagt
     3361 ggcgcagctg cttcatcccc gtggcccgtt gctcgcgttt gctggcggtg tccccggaag
     3421 aaatatattt gcatgtcttt agttctatga tgacacaaac cccgcccagc gtcttgtcat
     3481 tggcgaattc gaacacgcag atgcagtcgg ggcggcgcgg tcccaggtcc acttcgcata
     3541 ttaaggtgac gcgtgtggcc tcgaacaccg agcgaccctg cagcgacccg cttaaaagct
     3601 tgattcttct gacacaacag tctcgaactt aagctgcaga agttggtcgt gaggcactgg
     3661 gcaggtaagt atcaaggtta caagacaggt ttaaggagac caatagaaac tgggcttgtc
     3721 gagacagaga agactcttgc gtttctgata ggcacctatt ggtcttactg acatccactt
     3781 tgcctttctc tccacaggtg tccactccca gttcaattac agctcttaag gctagagtac
     3841 ttaatacgac tcactatagg ctagccacca tgacttcgaa agtttatgat ccagaacaaa
     3901 ggaaacggat gataactggt ccgcagtggt gggccagatg taaacaaatg aatgttcttg
     3961 attcatttat taattattat gattcagaaa aacatgcaga aaatgctgtt atttttttac
     4021 atggtaacgc ggcctcttct tatttatggc gacatgttgt gccacatatt gagccagtag
     4081 cgcggtgtat tataccagac cttattggta tgggcaaatc aggcaaatct ggtaatggtt
     4141 cttataggtt acttgatcat tacaaatatc ttactgcatg gtttgaactt cttaatttac
     4201 caaagaagat catttttgtc ggccatgatt ggggtgcttg tttggcattt cattatagct
     4261 atgagcatca agataagatc aaagcaatag ttcacgctga aagtgtagta gatgtgattg
     4321 aatcatggga tgaatggcct gatattgaag aagatattgc gttgatcaaa tctgaagaag
     4381 gagaaaaaat ggttttggag aataacttct tcgtggaaac catgttgcca tcaaaaatca
     4441 tgagaaagtt agaaccagaa gaatttgcag catatcttga accattcaaa gagaaaggtg
     4501 aagttcgtcg tccaacatta tcatggcctc gtgaaatccc gttagtaaaa ggtggtaaac
     4561 ctgacgttgt acaaattgtt aggaattata atgcttatct acgtgcaagt gatgatttac
     4621 caaaaatgtt tattgaatcg gacccaggat tcttttccaa tgctattgtt gaaggtgcca
     4681 agaagtttcc taatactgaa tttgtcaaag taaaaggtct tcatttttcg caagaagatg
     4741 cacctgatga aatgggaaaa tatatcaaat cgttcgttga gcgagttctc aaaaatgaac
     4801 aataattcta gagcggccgc ttcgagcaga catgataaga tacattgatg agcaacttta
     4861 ttatacatag ttgataattc actcgacaat caacctctgg attacaaaat ttgtgaaaga
     4921 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     4981 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     5041 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     5101 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     5161 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     5221 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     5281 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     5341 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     5401 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     5461 tgggccgcct ccccgcctgg aattcgagct cggtaccttt aagaccaatg acttacaagg
     5521 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     5581 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     5641 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5701 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     5761 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     5821 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     5881 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5941 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     6001 tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6061 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     6121 gtgaggaggc ttttttggag gcctaggctt ttgcgtcgag acgtacccaa ttcgccctat
     6181 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     6241 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     6301 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     6361 cgcgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     6421 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     6481 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     6541 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     6601 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     6661 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     6721 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     6781 tttaacgcga attttaacaa aatattaacg tttacaattt cccaggtggc acttttcggg
     6841 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     6901 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     6961 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7021 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7081 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7141 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7201 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7261 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7321 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7381 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7441 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7501 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7561 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     7621 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7681 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7741 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7801 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     7861 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7921 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7981 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8041 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8101 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     8161 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8221 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8281 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8341 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8401 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8461 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8521 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8581 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8641 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8701 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     8761 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     8821 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     8881 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     8941 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     9001 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
     9061 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     9121 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     9181 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
     9241 atttaagtgc ctagctcgat acaataaacg ggtctctctg gttagaccag atctgagcct
     9301 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     9361 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     9421 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggacc tgaaagcgaa
     9481 agggaaacca gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     9541 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     9601 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     9661 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     9721 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     9781 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     9841 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     9901 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     9961 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
    10021 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
    10081 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
    10141 cagcaggaag cactatgggc gcagcctcaa tgacgctgac ggtacaggcc agacaattat
    10201 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
    10261 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
    10321 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
    10381 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
    10441 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
    10501 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
    10561 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
    10621 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
    10681 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
    10741 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
    10801 ccattcgatt agtgaacgga tctcgacggt atcggttaac ttttaaaaga aaagggggga
    10861 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
    10921 aagaattaca aaaacaaatt acaaaaattc aaaattttat cgatcacgag actagcctc
//
