LOCUS       sequence-267285-        9032 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PHKG2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9032)
  AUTHORS   Galligan JT, Martinez-Noel G, Arndt V, Hayes S, Chittenden TW, 
            Harper JW, Howley PM
  TITLE     Proteomic analysis and identification of cellular interactors of the
            giant ubiquitin ligase HERC2.
  JOURNAL   J Proteome Res. 2015 Feb 6;14(2):953-66. doi: 10.1021/pr501005v. 
            Epub 2014 Dec 15.
  PUBMED    25476789
REFERENCE   2  (bases 1 to 9032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/pr501005v";
            journalName: "J Proteome Res"; date: "2015-02-6- 6"; volume: "14"; 
            issue: "2"; pages: "953-66"
COMMENT     Sequence Label: p7139 pHAGE-P-CMVt-N-HA-GAW-PHKG2
FEATURES             Location/Qualifiers
     source          1..9032
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(13..36)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     410..429
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             498..1097
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(498..517)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     994..1014
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    1164..1752
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1217..1237)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1635..1646)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1827..2060
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(2179..2197)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2265..2369
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2370..2438,2439..3230)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2588..2607)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3401..3989
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3890..3909
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4143..4160
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4400..4421
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4436..4441,4442..4459,4460..4466)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4474..4490
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4479..4501
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4498..4514
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4498..4514
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4564..4583
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             4775..5408
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5455..5580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6077..6310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6495..6539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6832..6949
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        7001..7304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7305..7508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7459..7479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    7510..7528
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7531..7549
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     7559..7583
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      7670..7679
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7682..7708
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    7724..7748
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8967..8991)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        join(9010..9032,1..477)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
ORIGIN
        1 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc
       61 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt
      121 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag
      181 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg
      241 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt
      301 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg
      361 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg
      421 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc
      481 tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
      541 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
      601 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
      661 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
      721 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
      781 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
      841 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
      901 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
      961 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     1021 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     1081 gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac
     1141 gaccccatgc atcgatagat cctaatcaac ctctggatta caaaatttgt gaaagattga
     1201 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     1261 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     1321 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     1381 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     1441 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     1501 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     1561 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     1621 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     1681 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     1741 ccgcctcccc gcctgagatc ctttaagacc aatgacttac aaggcagctg tagatcttag
     1801 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     1861 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     1921 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     1981 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     2041 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     2101 tgcaaagaaa tgaatatcag agagtgagag gcccgggtta attaaggaaa gggctagatc
     2161 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     2221 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     2281 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     2341 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     2401 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     2461 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     2521 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     2581 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
     2641 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     2701 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     2761 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     2821 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     2881 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     2941 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     3001 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     3061 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     3121 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     3181 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     3241 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     3301 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3361 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3421 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3481 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3541 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3601 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3661 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3721 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3781 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3841 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3901 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3961 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     4021 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     4081 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     4141 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     4201 cgcgttggcc gattcattaa tgcagcaagc tcatggctga ctaatttttt ttatttatgc
     4261 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     4321 aggcctaggc ttttgcaaaa agctccccgt ggcacgacag gtttcccgac tggaaagcgg
     4381 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     4441 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     4501 gaaacagcta tgacatgatt acgaatttca caaataaagc atttttttca ctgcattcta
     4561 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc
     4621 aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc
     4681 tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt
     4741 gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga
     4801 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag
     4861 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta
     4921 gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta
     4981 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt
     5041 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac
     5101 tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc
     5161 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
     5221 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     5281 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     5341 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     5401 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     5461 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     5521 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     5581 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     5641 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     5701 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     5761 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     5821 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     5881 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac
     5941 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     6001 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     6061 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     6121 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     6181 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     6241 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     6301 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     6361 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     6421 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     6481 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     6541 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     6601 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     6661 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     6721 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     6781 acggatctcg acggtatcgc cgaattcaca aatggcagta ttcatccaca attttaaaag
     6841 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     6901 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     6961 cagggacagc agagatccag tttggactag tggagttccg cgttacataa cttacggtaa
     7021 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     7081 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     7141 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     7201 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     7261 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     7321 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     7381 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     7441 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     7501 gcagagctct ccctatcagt gatagagatc tccctatcag tgatagagat cgtcgacgag
     7561 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     7621 gaagacaccg ggaccgatcc agcctccgga ctctagcgtt taaacgctag ccaccatggc
     7681 gtacccatac gatgttccag attacgctgc tcgaggttat caaacaagtt tgtacaaaaa
     7741 agttggcatg acgctggacg tggggccgga ggatgagctg cccgactggg ccgccgccaa
     7801 agagttttac cagaagtacg accctaagga cgtcatcggc agaggagtga gctctgtggt
     7861 ccgccgttgt gttcatcgag ctactggcca cgagtttgcg gtgaagatta tggaagtgac
     7921 agctgagcgg ctgagtcctg agcagctgga ggaggtgcgg gaagccacac ggcgagagac
     7981 acacatcctt cgccaggtcg ccggccaccc ccacatcatc accctcatcg attcctacga
     8041 gtcttctagc ttcatgttcc tggtgtttga cctgatgcgg aagggagagc tgtttgacta
     8101 tctcacagag aaggtggccc tctctgaaaa ggaaaccagg tccatcatgc ggtctctgct
     8161 ggaagcagtg agctttctcc atgccaacaa cattgtgcat cgagatctga agcccgagaa
     8221 tattctccta gatgacaata tgcagatccg actttcagat ttcgggttct cctgccactt
     8281 ggaacctggc gagaagcttc gagagttgtg tgggacccca gggtatctag cgccagagat
     8341 ccttaaatgc tccatggatg aaacccaccc aggctatggc aaggaggtcg acctctgggc
     8401 ctgtggggtg atcttgttca cactcctggc tggctcgcca cccttctggc accggcggca
     8461 gatcctgatg ttacgcatga tcatggaggg ccagtaccag ttcagttccc ccgagtggga
     8521 tgaccgttcc agcactgtca aagacctgat ctccaggctg ctgcaggtgg atcctgaggc
     8581 acgcctgaca gctgagcagg ccctacagca ccccttcttt gagcgttgtg aaggcagcca
     8641 accctggaac ctcacccccc gccagcggtt ccgggtggca gtgtggacag tgctggctgc
     8701 tggacgagtg gccctaagca cccatcgtgt acggccactg accaagaatg cactgttgag
     8761 ggacccttat gcgctgcggt cagtgcggca cctcatcgac aactgtgcct tccggctcta
     8821 cgggcactgg gtaaagaaag gggagcagca gaaccgggcg gctctctttc agcaccggcc
     8881 ccctgggcct tttcccatca tgggccctga agaggaggga gactctgctg ctataactga
     8941 ggatgaggcc gtgcttgtgc tgggctaccc aactttcttg tacaaagtgg ttcgataacg
     9001 aattctaccg ggtaggggag gcgcttttcc ca
//
