LOCUS       sequence-267317-        7964 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length UL87 3L_A with a C-terminal 2xStrep tag in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7964)
  AUTHORS   Castaneda AF, Didychuk AL, Louder RK, McCollum CO, Davis ZH, Nogales
            E, Glaunsinger BA
  TITLE     The gammaherpesviral TATA-box-binding protein directly interacts 
            with the CTD of host RNA Pol II to direct late gene transcription.
  JOURNAL   PLoS Pathog. 2020 Sep 4;16(9):e1008843. doi: 
            10.1371/journal.ppat.1008843. eCollection 2020 Sep.
  PUBMED    32886723
REFERENCE   2  (bases 1 to 7964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2020-09-4"; volume: "16(9):e1008843. doi"; pages: "
            10.1371/journal.ppat.1008843. eCollection 2020 Sep"
COMMENT     Sequence Label: pcDNA4/TO-UL87 3L_A-2xStrep
FEATURES             Location/Qualifiers
     source          1..7964
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3866..3889
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3926..3949
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(3975..3992)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3981..4205
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4251..4679
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4338..4357)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4548..4569
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4688..4708)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4693..5022
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4873..5008
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4935..4954
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5070..5117
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5136..5510
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5640..5761
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5677..5696)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5731..5750
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5810..5826)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5810..5826)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5823..5845)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5834..5850
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5858..5864,5865..5882,5883..5888))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5903..5924
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6041..6058)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6212..6797)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6292..6311)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6968..7759,7760..7828))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7591..7610
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7829..7933)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggaacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc cactagtcca gtgtggtgga
     1021 attcatggcc ggcgctgcgc cgcgccgcct cggctgcgac gctctaatag tcgtcggcgg
     1081 ctccgctacg ccgcgccggg ttttacacgt ccccgtgcac gttcgcgcct gcaacctcac
     1141 ccaagagcta tcgacgggcg aggacgcccg cttctgtcgt ccgcgacccg ttaacgtcga
     1201 acgggtacgc gctgttttcg cggctctcta ccgtgcctgt cccgtacacg cgaggaccga
     1261 gtccgagcgt gtcaagctgg tactgggtcg tgccgcggca ggacccgtgg ccgtaccctg
     1321 tttttgcgac ggtgaagtgg agggccacgg cgagcatctg gtacccacga cgcagttttg
     1381 tcgcgggccg ctgctctacg tgcaccgacg ttgttgttgc ggatccgtga ccgccgggcg
     1441 cgcgctgtcc taccacgttc tcgaaaacca cgtggccacg catgtgttac gcggattgct
     1501 ctcgctgacg gaatggaatc gagagttgcc gggccttttt tgcgactgtc ctggcagcgg
     1561 tggcgccttg ggaaccgagg aacgctacgc catggcctgc ttgccgcgcg acctcagcct
     1621 gcacctggac gactatcctt acctgatggt ggaaatcgga cgcgtactca gcgtcagcga
     1681 ggtagacgac tacgtaaccg ccgtctccgg ctacctgggc gaggccgcgg cgccgcgcat
     1741 tcaggttcac tacaagctgc tctttggact caacgtgcgt ccgcaagcgc cgtgcgcgtt
     1801 ggacgctaca cgcgactttt ttctgctgga gctgcaaaag ctttggctgg gcgttgaata
     1861 tcaccacgaa gtcacgtcgg agtttttcgg tcgcgtactg gctcagctgc atcgcgaccg
     1921 cgcccgcgtc atgatggcac ttcgcttgcc cgagcagacg gtgtgccacc taagcacctt
     1981 cgttctcagt cgcttcaagc gacaggtact gtacttcaag ttacaggtga gctacggcaa
     2041 gtgccggact ggccacgctg acagaagtgg gggaggggga aacggtggaa gtcagggaca
     2101 ccacaaccta ctgtgttatc gacgtcttag cgtcacgttt gccgacacgg acacggtgtg
     2161 gagaaacctt ttctacgttt attatgaact agctcgggat ctggggtccc atgggacaga
     2221 ggaccgatcc gtaagccgcg gttacggtgt ttcttgcgct ccgaggacgt cgcggctacc
     2281 accgtcagaa ccgacggtgg tttcagccaa cggacacgcg ctgtcttcca ccgcgctccc
     2341 gacgacgagc gcgggtcaca agctgtcgct gccgcgcgac ccggccgcag atcgcgttcg
     2401 acgttacgtg tgcattatct cgcgtctcat gttcgcccgg tatggggaga gatggcgtaa
     2461 acaccgtcga cggcggtcgg agacgggaga agaggaggag gaagagacgg tggaatcggg
     2521 ggagactgac gccacgccgc catttgactt tacggggcag cagctgcgcc gggcctatca
     2581 ggaacaccga cgtcgtaaac atctagccgt gcagcgttac gcgccgtgcc gtcgtaagct
     2641 catcggcggg atggagtttg ccgaggtgac gggcgttagt ctggaccgca tcgccgtcaa
     2701 cgctttcaac accaaccgcg ttatcaatat gaaggccgca ctctcgtcca tcgccgcgtc
     2761 gggtctcggc gtgcgcgcgc cgcggcttcc caagaacatg acccacagtt ttgtgatgta
     2821 caagcacacc ttcaaggagc ccgcttgcac cgtcagcact tttgtttcca acgacgccgt
     2881 ctacatcaac tcgctcaacg tcaatattcg cggttcctat cccgagtttc tgtactcgct
     2941 gggcgtgtac cggctgcacg ttaatatcga tcacttcttt ctgccggccg tggtgtgcaa
     3001 cagcaactcc tcgctggacg tgcatgggct ggaggaccag gcggtgatcc gctcggagcg
     3061 cagcaaggtg tactggacca ccaactttcc gtgcatgatc tcgcatacta acaacgtcaa
     3121 cgtgggttgg ttcaaagcgg ctacggccat tgtgccgcgc gtctcgggcg ctgacctgga
     3181 agccattctg ctcaaagaac tctcgtgcat caagaacatg cgcgacgtgt gcatcgatta
     3241 cggtctgcat cgcgttttca cgcaactaga gctgcgcaat tcgtaccaga tccccttcct
     3301 ggccaagcag ttggtgctgt ttctgcgtgc ttgcctgctc aagctgcacg gtcgagagaa
     3361 gcggctgcag ttggaccgcc tagtatttga ggcggcacag cggggtctct ttgactacag
     3421 caagaacctc acggcgcaca ccaagatcaa gcacacttgc gcgctcatcg gcagtcgtct
     3481 agccaacaac gtgcccaaga tcctggcccg gaacaaaaaa gtcaaattgg atcacctggg
     3541 ccggaacgcc aacgtgctga cggtgtgtcg gcacgtggaa gcccacaaga tccctcgcac
     3601 gcgcctcaaa gtgttagtcg aggtgctggg cgcgttgcag agtatcagcg gtacgccgca
     3661 cacgcgtgaa gtgatccacc agacgttgtt tcgattgtgc tcggcggccg cagccacctc
     3721 gggcctgtgt tcatcccctc ccccattgtg tgtgtcctca tcttcctccg ccccttctgt
     3781 cccaacctcc gtcagcgttg acggcagttc tgaacccacg tcgccgcgag cgcggtttgc
     3841 atcacgactc gagggaggcg gtggatggag ccacccccag ttcgagaagg gcggcggcag
     3901 cggcggcggc agcggcggcg gcagctggag ccacccccag ttcgagaagg ggcccgttta
     3961 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     4021 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     4081 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     4141 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     4201 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     4261 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4321 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4381 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4441 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     4501 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     4561 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     4621 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     4681 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     4741 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     4801 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     4861 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     4921 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     4981 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     5041 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg
     5101 gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc
     5161 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
     5221 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
     5281 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
     5341 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
     5401 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
     5461 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac
     5521 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     5581 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     5641 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     5701 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     5761 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     5821 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     5881 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     5941 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     6001 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     6061 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6121 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6181 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6241 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6301 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6361 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6421 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6481 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6541 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6601 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     6661 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6721 ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca
     6781 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     6841 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     6901 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     6961 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7021 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7081 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7141 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7201 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7261 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7321 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7381 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7441 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     7501 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     7561 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     7621 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     7681 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     7741 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     7801 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     7861 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     7921 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
