LOCUS       sequence-267318-        7281 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length mu24 3L_A with a C-terminal 2xStrep tag in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7281)
  AUTHORS   Castaneda AF, Didychuk AL, Louder RK, McCollum CO, Davis ZH, Nogales
            E, Glaunsinger BA
  TITLE     The gammaherpesviral TATA-box-binding protein directly interacts 
            with the CTD of host RNA Pol II to direct late gene transcription.
  JOURNAL   PLoS Pathog. 2020 Sep 4;16(9):e1008843. doi: 
            10.1371/journal.ppat.1008843. eCollection 2020 Sep.
  PUBMED    32886723
REFERENCE   2  (bases 1 to 7281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2020-09-4"; volume: "16(9):e1008843. doi"; pages: "
            10.1371/journal.ppat.1008843. eCollection 2020 Sep"
COMMENT     Sequence Label: pcDNA4/TO-mu24 3L_A-2xStrep
FEATURES             Location/Qualifiers
     source          1..7281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2408..2425
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     CDS             3183..3206
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3243..3266
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(3292..3309)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3298..3522
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3568..3996
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3655..3674)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3865..3886
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4005..4025)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4010..4339
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4190..4325
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4252..4271
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4387..4434
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4453..4827
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4957..5078
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4994..5013)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5048..5067
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5127..5143)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5127..5143)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5140..5162)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5151..5167
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5175..5181,5182..5199,5200..5205))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5220..5241
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5358..5375)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5529..6114)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5609..5628)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6285..7076,7077..7145))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6908..6927
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7146..7250)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggaacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc catgacaata ttcttaccag
     1021 tattctgtga tttgccagaa gtataccaag attgtgaata tgatgagaat agccaactgt
     1081 ctatcttatt ctggatatca tccaatacat atgaggtagt agagaatgta caacaattta
     1141 atatacttgg taaagattta acattctgtt tatgtagagc tttaagaaga gccgcggcag
     1201 gcataagact gtacccaacc tgcaatactg agtctaacag atatgtggtt accggggaca
     1261 gatatgtggg caatggtttg gtggttagtc ccacaggtag gcatattcag cgcatagata
     1321 cctgcaccta tgttccagtg atatacagct ttgaacaaac tgacgcacat tatgatggca
     1381 tgggacctgg aaaactgagg gccttgtatc atgagcaatt attttttaaa ggaattgatt
     1441 attcctttgt attttccacc ctcatcagat ttttatctat gaaacagata gatgagtgtt
     1501 actactcttt tatccatact ttggaaccct gggccaggtc tgcttgtaaa aaaaattact
     1561 tgaagcttac tgagtgtttt aaaaatgtgg ccctcacaaa gttgcctgtg gttgatgcca
     1621 cctttcattt ggaatatatc aaattcaata tcaaatgttt ccttgaccaa tggaatgact
     1681 gccccgacct ttgggtactt agaagaacca tctctgcgaa tttatccaaa aaacacctgc
     1741 tggccactat tggaagactc atgtctgtgt gtgagatcca gcttaggaac cccttagcct
     1801 atcatgatca cctaactatg atgaaaacat ttccacaagt gacaatatct gctcagaaga
     1861 aacccaccac ctgtttaaat cagaaactgg aaatagtcat gtcaggtaca gagtccatgt
     1921 ggctggtata cactcctgct tctagtatgt ttcgaattat gctctgtgcc gcctacatca
     1981 gcgacatcta ctacaacaat actgtgcagc aggcaattta tgatgtcacc cacgataagc
     2041 caagcaacgg tgagatgttg atgaacttgt atagaagaat tgattacctg cctaaagact
     2101 cagtagaaac tattgcaaga gtgtgttcca ccccctggga gaacccccta attgaaaggc
     2161 cgcctaccat gtatcatgaa tactgtccaa tcaaaaatct tagcatcaat agtttcaagg
     2221 tcacaatttt taatacaaat atggtcatca acaccaaaat ctcatgcaaa aagatcaccc
     2281 cccgatttag atcattcttg gatatcccta ggctcaccaa taactttgtt gtgaaaaagt
     2341 attctgttaa agaaccatca tttaccataa gtgtgttcta ttctgatgat ttatgtaaaa
     2401 gtggtgccat caacatcaac atcagtggta cactaataca cttcctcttt gctatgggct
     2461 gcctaaaatg cttcatgccc atcaaaacta taaatcctgt cctgatatcc aactggaact
     2521 caacctttga tcttcaggga ctagaaaatc aagagattgt cagaactggc aggcatgatg
     2581 tgttttggac caccaacttc ccatcagcgg tctctaccaa gcgcgggtac aacatttcct
     2641 ggtttaaggc cgccaccgcc accgtctcca agatccacgg cggagcgctg ctagatcaag
     2701 tgagggggga aatttcacag atattactca gcagtgatgc aattataaat accaacaaaa
     2761 attgcatcta cactacactg gaaaagcgaa accgctttca gattcaaacc ttgcacaaaa
     2821 gattcctaga gtgcttgttt gagtcatgtg tggctagcaa acttaattat catacagtga
     2881 aaaggctatg ttctacaggc attttcgatt tcagtagaca tataattagt cattcaaaaa
     2941 acaagcatga atgtgcactg tatggctacc gaaagtgtaa tcttgtacca aaaatattaa
     3001 caggtacaaa aaagactcgc ctggacgagc tggggcggaa ttccaattat ctttcattca
     3061 ataaatcccc acttcaccat aaaaaggatg cttcggattt taaaaaaaga ctgtgcctta
     3121 acaggaggag aagtaaccct tgccaaccag actccgggcg gccgctcgag ggaggcggtg
     3181 gatggagcca cccccagttc gagaagggcg gcggcagcgg cggcggcagc ggcggcggca
     3241 gctggagcca cccccagttc gagaaggggc ccgtttaaac ccgctgatca gcctcgactg
     3301 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     3361 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     3421 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     3481 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     3541 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     3601 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     3661 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     3721 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     3781 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     3841 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     3901 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     3961 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     4021 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     4081 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4141 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     4201 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4261 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     4321 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     4381 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
     4441 aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc
     4501 gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac
     4561 gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag
     4621 gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc
     4681 gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag
     4741 atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg
     4801 cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc
     4861 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     4921 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     4981 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     5041 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     5101 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     5161 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     5221 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     5281 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     5341 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     5401 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     5461 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     5521 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     5581 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     5641 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     5701 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     5761 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     5821 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     5881 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     5941 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     6001 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     6061 agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     6121 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     6181 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     6241 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     6301 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     6361 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     6421 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     6481 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     6541 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     6601 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     6661 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     6721 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     6781 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     6841 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     6901 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     6961 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     7021 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     7081 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     7141 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     7201 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     7261 cgaaaagtgc cacctgacgt c
//
