LOCUS       sequence-267333-        7341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mu24-ORF24 chimera with a C-terminal 2xStrep tag in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7341)
  AUTHORS   Castaneda AF, Didychuk AL, Louder RK, McCollum CO, Davis ZH, Nogales
            E, Glaunsinger BA
  TITLE     The gammaherpesviral TATA-box-binding protein directly interacts 
            with the CTD of host RNA Pol II to direct late gene transcription.
  JOURNAL   PLoS Pathog. 2020 Sep 4;16(9):e1008843. doi: 
            10.1371/journal.ppat.1008843. eCollection 2020 Sep.
  PUBMED    32886723
REFERENCE   2  (bases 1 to 7341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2020-09-4"; volume: "16(9):e1008843. doi"; pages: "
            10.1371/journal.ppat.1008843. eCollection 2020 Sep"
COMMENT     Sequence Label: pcDNA4/TO-mu24-ORF24-2xStrep
FEATURES             Location/Qualifiers
     source          1..7341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3243..3266
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3303..3326
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(3352..3369)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3358..3582
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3628..4056
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3715..3734)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3925..3946
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4065..4085)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4070..4399
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4250..4385
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4312..4331
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4447..4494
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4513..4887
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5017..5138
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5054..5073)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5108..5127
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5187..5203)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5187..5203)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5200..5222)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5211..5227
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5235..5241,5242..5259,5260..5265))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5280..5301
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5418..5435)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5589..6174)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5669..5688)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6345..7136,7137..7205))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6968..6987
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7206..7310)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggaacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc catgacaata ttcttaccag
     1021 tattctgtga tttgccagaa gtataccaag attgtgaata tgatgagaat agccaactgt
     1081 ctatcttatt ctggatatca tccaatacat atgaggtagt agagaatgta caacaattta
     1141 atatacttgg taaagattta acattctgtt tatgtagagc tttaagaaga ctgctcctgg
     1201 gcataagact gtacccaacc tgcaatactg agtctaacag atatgtggtt accggggaca
     1261 gatatgtggg caatggtttg gtggttagtc ccacaggtag gcatattcag cgcatagata
     1321 cctgcaccta tgttccagtg atatacagct ttgaacaaac tgacgcacat tatgatggca
     1381 tgggacctgg aaaactgagg gccttgtatc atgagcaatt attttttaaa ggaattgatt
     1441 attcctttgt attttccacc ctcatcagat ttttatctat gaaacagata gatgagtgtt
     1501 actactcttt tatccatact ttggaaccct gggccaggtc tgcttgtaaa aaaaattact
     1561 tgaagcttac tgagagcctg aagcatctct cgttttcaat aatgccaccc gcatccccag
     1621 acgcacagct acatttttta aagtttaaca tcggcagctt catggccacg tgggggtggc
     1681 acggagagct ggtctcgctg cgccgtgcca tcgctcacaa cgtagagcga ctgcccaccg
     1741 tgctgaagaa cctgtcgaaa cagagtaagc accaggacgt caaggttaac ggacgggatc
     1801 tggtgggctt tcagctggct ctaaaccagc tcgtgtcccg tctgcacgta aaaatccaac
     1861 gcaaggaccc cggaccaaag ccatacaggg tggtcgtcag taccccagat tgtacctact
     1921 atctagtgta tccgggcaca ccggccatct acagactcgt catgtgtatg gcagtggcag
     1981 actgcatcgg ccactcgtgc agcggactgc acccctgcgc aaacttttta ggcacccacg
     2041 agacaccgcg tctcctggcg gcgacgcttt caagaatccg gtacgcgccg aaagaccggc
     2101 gagcagccat gaagggaaat ttgcaggcgt gcttccaacg atacgcggcc acggacgcgc
     2161 ggactctggg cagctccaca gtgtcagaca tgctggaacc cacaaaacac gtcagtttgg
     2221 aaaacttcaa gatcaccata ttcaacacca acatggtgat taacactaag ataagctgcc
     2281 acgttcctaa caccctgcaa aagactattt taaacatccc cagattgacc aacaattttg
     2341 ttatacgaaa gtactccgta aaggaacctt cttttaccat aagcgtgttt ttttccgaca
     2401 acatgtgtca aggcaccgca ataaacatca acatcagtgg ggacatgctg cactttctct
     2461 tcgcaatggg tacgctgaaa tgctttctgc caatcaggca catatttcct gtatcgatag
     2521 caaattggaa ctccacgttg gacctgcacg gactggaaaa ccagtacatg gtgagaatgg
     2581 ggcgaaaaaa cgtattttgg accacaaact ttccatctgt ggtctccagc aaggatgggc
     2641 taaacgtgtc ctggtttaag gccgcgacag ccacgatttc taaagtgtac gggcagcctc
     2701 ttgtggaaca gattcgccac gagctggcgc ccattctcac ggaccagcac gcgcgcatcg
     2761 acggaaacaa aaatagaata ttctccctac ttgagcacag aaaccgttcc caaatacaga
     2821 cgctacacaa aaggttcctg gagtgtctgg tggaatgctg ttcgtttctc aggcttgacg
     2881 tggcttgcat taggcgagcc gccgcccggg gcctgtttga cttctcaaag aagataatca
     2941 gtcacactaa aagcaaacac gagtgcgcag tactgggata taaaaagtgt aacctaatcc
     3001 cgaaaatcta tgcccgaaac aagaagacca ggctagacga gttgggccgc aatgcaaact
     3061 tcatttcgtt cgtcgccacc acgggtcatc ggttcgccgc tctaaagcca caaattgtcc
     3121 gtcacgccat tcgcaaacta ggcctgcact ggcgccaccg aacggccgcg tccaacgagc
     3181 agacaccgcc agccgatccc cgcgtacgtt gcgtccgtcc gctgctcgag ggaggcggtg
     3241 gatggagcca cccccagttc gagaagggcg gcggcagcgg cggcggcagc ggcggcggca
     3301 gctggagcca cccccagttc gagaaggggc ccgtttaaac ccgctgatca gcctcgactg
     3361 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     3421 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     3481 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     3541 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     3601 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     3661 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     3721 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     3781 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     3841 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     3901 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     3961 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     4021 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     4081 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     4141 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4201 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     4261 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4321 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     4381 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     4441 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
     4501 aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc
     4561 gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac
     4621 gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag
     4681 gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc
     4741 gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag
     4801 atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg
     4861 cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc
     4921 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     4981 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     5041 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     5101 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     5161 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     5221 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     5281 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     5341 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     5401 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     5461 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     5521 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     5581 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     5641 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     5701 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     5761 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     5821 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     5881 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     5941 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     6001 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     6061 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     6121 agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     6181 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     6241 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     6301 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     6361 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     6421 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     6481 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     6541 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     6601 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     6661 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     6721 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     6781 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     6841 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     6901 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     6961 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     7021 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     7081 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     7141 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     7201 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     7261 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     7321 cgaaaagtgc cacctgacgt c
//
