LOCUS       sequence-267335-        7512 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses UL87-ORF24 chimera with a C-terminal 2xStrep tag in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7512)
  AUTHORS   Castaneda AF, Didychuk AL, Louder RK, McCollum CO, Davis ZH, Nogales
            E, Glaunsinger BA
  TITLE     The gammaherpesviral TATA-box-binding protein directly interacts 
            with the CTD of host RNA Pol II to direct late gene transcription.
  JOURNAL   PLoS Pathog. 2020 Sep 4;16(9):e1008843. doi: 
            10.1371/journal.ppat.1008843. eCollection 2020 Sep.
  PUBMED    32886723
REFERENCE   2  (bases 1 to 7512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2020-09-4"; volume: "16(9):e1008843. doi"; pages: "
            10.1371/journal.ppat.1008843. eCollection 2020 Sep"
COMMENT     Sequence Label: pcDNA4/TO-UL87-ORF24-2xStrep
FEATURES             Location/Qualifiers
     source          1..7512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3414..3437
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3474..3497
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(3523..3540)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3529..3753
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3799..4227
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3886..3905)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4096..4117
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4236..4256)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4241..4570
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4421..4556
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4483..4502
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4618..4665
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4684..5058
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5188..5309
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5225..5244)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5279..5298
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5358..5374)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5358..5374)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5371..5393)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5382..5398
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5406..5412,5413..5430,5431..5436))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5451..5472
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5589..5606)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5760..6345)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5840..5859)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6516..7307,7308..7376))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7139..7158
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7377..7481)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggaacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc catggccggc gctgcgccgc
     1021 gccgcctcgg ctgcgacgct ctaatagtcg tcggcggctc cgctacgccg cgccgggttt
     1081 tacacgtccc cgtgcacgtt cgcgcctgca acctcaccca agagctatcg acgggcgagg
     1141 acgcccgctt ctgtcgtccg cgacccgtta acgtcgaacg ggtacgcgct gttttcgcgg
     1201 ctctctaccg tgcctgtccc gtacacgcga ggaccgagtc cgagcgtgtc aagctggtac
     1261 tgggtcgtct gttgctggga cccgtggccg taccctgttt ttgcgacggt gaagtggagg
     1321 gccacggcga gcatctggta cccacgacgc agttttgtcg cgggccgctg ctctacgtgc
     1381 accgacgttg ttgttgcgga tccgtgaccg ccgggcgcgc gctgtcctac cacgttctcg
     1441 aaaaccacgt ggccacgcat gtgttacgcg gattgctctc gctgacggaa tggaatcgag
     1501 agttgccggg ccttttttgc gactgtcctg gcagcggtgg cgccttggga accgaggaac
     1561 gctacgccat ggcctgcttg ccgcgcgacc tcagcctgca cctggacgac tatccttacc
     1621 tgatggtgga aatcggacgc gtactcagcg tcagcgaggt agacgactac gtaaccgccg
     1681 tctccggcta cctgggcgag gccgcggcgc cgcgcattca ggttcactac aagctgctct
     1741 ttggaagcct gaagcatctc tcgttttcaa taatgccacc cgcatcccca gacgcacagc
     1801 tacatttttt aaagtttaac atcggcagct tcatggccac gtgggggtgg cacggagagc
     1861 tggtctcgct gcgccgtgcc atcgctcaca acgtagagcg actgcccacc gtgctgaaga
     1921 acctgtcgaa acagagtaag caccaggacg tcaaggttaa cggacgggat ctggtgggct
     1981 ttcagctggc tctaaaccag ctcgtgtccc gtctgcacgt aaaaatccaa cgcaaggacc
     2041 ccggaccaaa gccatacagg gtggtcgtca gtaccccaga ttgtacctac tatctagtgt
     2101 atccgggcac accggccatc tacagactcg tcatgtgtat ggcagtggca gactgcatcg
     2161 gccactcgtg cagcggactg cacccctgcg caaacttttt aggcacccac gagacaccgc
     2221 gtctcctggc ggcgacgctt tcaagaatcc ggtacgcgcc gaaagaccgg cgagcagcca
     2281 tgaagggaaa tttgcaggcg tgcttccaac gatacgcggc cacggacgcg cggactctgg
     2341 gcagctccac agtgtcagac atgctggaac ccacaaaaca cgtcagtttg gaaaacttca
     2401 agatcaccat attcaacacc aacatggtga ttaacactaa gataagctgc cacgttccta
     2461 acaccctgca aaagactatt ttaaacatcc ccagattgac caacaatttt gttatacgaa
     2521 agtactccgt aaaggaacct tcttttacca taagcgtgtt tttttccgac aacatgtgtc
     2581 aaggcaccgc aataaacatc aacatcagtg gggacatgct gcactttctc ttcgcaatgg
     2641 gtacgctgaa atgctttctg ccaatcaggc acatatttcc tgtatcgata gcaaattgga
     2701 actccacgtt ggacctgcac ggactggaaa accagtacat ggtgagaatg gggcgaaaaa
     2761 acgtattttg gaccacaaac tttccatctg tggtctccag caaggatggg ctaaacgtgt
     2821 cctggtttaa ggccgcgaca gccacgattt ctaaagtgta cgggcagcct cttgtggaac
     2881 agattcgcca cgagctggcg cccattctca cggaccagca cgcgcgcatc gacggaaaca
     2941 aaaatagaat attctcccta cttgagcaca gaaaccgttc ccaaatacag acgctacaca
     3001 aaaggttcct ggagtgtctg gtggaatgct gttcgtttct caggcttgac gtggcttgca
     3061 ttaggcgagc cgccgcccgg ggcctgtttg acttctcaaa gaagataatc agtcacacta
     3121 aaagcaaaca cgagtgcgca gtactgggat ataaaaagtg taacctaatc ccgaaaatct
     3181 atgcccgaaa caagaagacc aggctagacg agttgggccg caatgcaaac ttcatttcgt
     3241 tcgtcgccac cacgggtcat cggttcgccg ctctaaagcc acaaattgtc cgtcacgcca
     3301 ttcgcaaact aggcctgcac tggcgccacc gaacggccgc gtccaacgag cagacaccgc
     3361 cagccgatcc ccgcgtacgt tgcgtccgtc cgctgctcga gggaggcggt ggatggagcc
     3421 acccccagtt cgagaagggc ggcggcagcg gcggcggcag cggcggcggc agctggagcc
     3481 acccccagtt cgagaagggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     3541 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     3601 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     3661 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     3721 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     3781 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     3841 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     3901 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     3961 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     4021 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4081 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     4141 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     4201 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     4261 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4321 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     4381 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     4441 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     4501 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     4561 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt
     4621 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa
     4681 accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg
     4741 gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc
     4801 ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg
     4861 gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg
     4921 gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag
     4981 cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg
     5041 gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa
     5101 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     5161 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     5221 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     5281 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     5341 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     5401 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     5461 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     5521 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     5581 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5641 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5701 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5761 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5821 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5881 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5941 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6001 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6061 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6121 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6181 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6241 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt
     6301 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     6361 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     6421 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     6481 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     6541 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     6601 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     6661 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     6721 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     6781 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     6841 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     6901 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     6961 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     7021 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     7081 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     7141 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     7201 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     7261 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     7321 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     7381 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     7441 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     7501 ccacctgacg tc
//
