LOCUS       sequence-267341-        9905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enables high-efficiency recombineering in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9905)
  AUTHORS   Wannier TM, Nyerges A, Kuchwara HM, Czikkely M, Balogh D, Filsinger 
            GT, Borders NC, Gregg CJ, Lajoie MJ, Rios X, Pal C, Church GM
  TITLE     Improved bacterial recombineering by parallelized protein discovery.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Jun 16;117(24):13689-13698. doi: 
            10.1073/pnas.2001588117. Epub 2020 May 28.
  PUBMED    32467157
REFERENCE   2  (bases 1 to 9905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.2001588117"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2020-06-16- 16"; volume: "117"; issue: "24"; pages: 
            "13689-13698"
COMMENT     Sequence Label: pORTMAGE-Ec1
FEATURES             Location/Qualifiers
     source          1..9905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1938..2032
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(2153..2172)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2164..2979
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            3146..3254
                     /note="incP origin of transfer"
     CDS             complement(3265..4116)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(4103..4942)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(5453..6424)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     rep_origin      6531..6925
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(6963..7049)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(7074..8039)
                     /codon_start=1
                     /gene="xylS"
                     /product="XylS regulator encoded by the Pseudomonas putida 
                     TOL plasmid pWWO"
                     /label=XylS
                     /note="stimulates transcription from the Pm promoter in the
                     presence of aromatic compounds such as m-toluate or 
                     benzoate"
                     /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGRPAGR
                     LHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGELLL
                     LNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDGFIN
                     LLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQFIEE
                     NLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPSANVRS
                     ITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA"
     promoter        8939..9019
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
ORIGIN
        1 gatccaggag aatacacatg ccaattcagg tcttaccgcc acaactggcg aaccagattg
       61 ccgcaggtga ggtggtcgag cgacctgcgt cggtagtcaa agaactagtg aaaaacagcc
      121 tcgatgcagg tgcgacgcgt atcgatattg atatcgaacg cggtggggcg aaacttatcc
      181 gcattcgtga taacggctgc ggtatcaaaa aagatgagct ggcgctggcg ctggctcgtc
      241 atgccaccag taaaatcgcc tctctggacg atctcgaagc cattatcagc ctgggctttc
      301 gcggtgaggc gctggcgagt atcagttcgg tttcccgcct gacgctcact tcacgcaccg
      361 cagaacagca ggaagcctgg caggcctatg ccgaagggcg cgatatgaac gtgacggtaa
      421 aaccggcggc gcatcctgtg gggacgacgc tggaggtgct ggatctgttc tacaacaccc
      481 cggcgcggcg caaattcctg cgcaccgaga aaaccgaatt taaccacatt gatgagatca
      541 tccgccgcat tgcgctggcg cgtttcgacg tcacgatcaa cctgtcgcat aacggtaaaa
      601 ttgtgcgtca gtaccgcgca gtgccggaag gcgggcaaaa agaacggcgc ttaggcgcga
      661 tttgcggcac cgcttttctt gaacaagcgc tggcgattga atggcaacac ggcgatctca
      721 cgctacgcgg ctgggtggcc gatccaaatc acaccacgcc cgcactggca gaaattcagt
      781 attgctacgt gaacggtcgc atgatgcgcg atcgcctgat caatcacgcg atccgccagg
      841 cctgcgaaga caaactgggg gccgatcagc aaccggcatt tgtgttgtat ctggagatcg
      901 acccacatca ggtggacgtc aacgtgcacc ccgccaaaca cgaagtgcgt ttccatcagt
      961 cgcgtctggt gcatgatttt atctatcagg gcgtgctgag cgtgctacaa cagcaactgg
     1021 aaacgccgct accgctggac gatgaacccc aacctgcacc gcgttccatt ccggaaaacc
     1081 gcgtggcggc ggggcgcaat cactttgcag aaccggcagc tcgtgagccg gtagctccgc
     1141 gctacactcc tgcgccagca tcaggcagtc gtccggctgc cccctggccg aatgcgcagc
     1201 caggctacca gaaacagcaa ggtgaagtgt atcgccagct tttgcaaacg cccgcgccga
     1261 tgcaaaaatt aaaagcgccg gaaccgcagg aacctgcact tgcggcgaac agtcagagtt
     1321 ttggtcgggt actgactatc gtccattccg actgtgcgtt gctggagcgc gacggcaaca
     1381 tttcactttt atccttgcca gtggcagaac gttggctgcg tcaggcacaa ttgacgccgg
     1441 gtgaagcgcc cgtttgcgcc cagccgctgc tgattccgtt gcggctaaaa gtttctgccg
     1501 aagaaaaatc ggcattagaa aaagcgcagt ctgccctggc ggaattgggt attgatttcc
     1561 agtcagatgc acagcatgtg accatcaggg cagtgccttt acccttacgc caacaaaatt
     1621 tacaaatctt gattcctgaa ctgataggct acctggcgaa gcagtccgta ttcgaacctg
     1681 gcaatattgc gcagtggatt gcacgaaatc tgatgagcga acatgcgcag tggtcaatgg
     1741 cacaggccat aaccctgctg gcggacgtgg aacggttatg tccgcaactt gtgaaaacgc
     1801 cgccgggtgg tctgttacaa tctgttgatt tacatccggc gataaaagcc ctgaaagatg
     1861 agtgatctag agtcgacctg caggcatgca agcttgcggc cgcgtcgtga ctgggaaaac
     1921 cctggcgact agtcttggac tcctgttgat agatccagta atgacctcag aactccatct
     1981 ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt
     2041 ccccaataat tacgatttaa atttgtgtct caaaatctct gatgttacat tgcacaagat
     2101 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     2161 gttatgagcc atattcagcg tgaaacgagc tgtagccgtc cgcgtctgaa cagcaacatg
     2221 gatgcggatc tgtatggcta taaatgggcg cgtgataacg tgggtcagag cggcgcgacc
     2281 atttatcgtc tgtatggcaa accggatgcg ccggaactgt ttctgaaaca tggcaaaggc
     2341 agcgtggcga acgatgtgac cgatgaaatg gtgcgtctga actggctgac cgaatttatg
     2401 ccgctgccga ccattaaaca ttttattcgc accccggatg atgcgtggct gctgaccacc
     2461 gcgattccgg gcaaaaccgc gtttcaggtg ctggaagaat atccggatag cggcgaaaac
     2521 attgtggatg cgctggccgt gtttctgcgt cgtctgcata gcattccggt gtgcaactgc
     2581 ccgtttaaca gcgatcgtgt gtttcgtctg gcccaggcgc agagccgtat gaacaacggc
     2641 ctggtggatg cgagcgattt tgatgatgaa cgtaacggct ggccggtgga acaggtgtgg
     2701 aaagaaatgc ataaactgct gccgtttagc ccggatagcg tggtgaccca cggcgatttt
     2761 agcctggata acctgatttt cgatgaaggc aaactgattg gctgcattga tgtgggccgt
     2821 gtgggcattg cggatcgtta tcaggatctg gccattctgt ggaactgcct gggcgaattt
     2881 agcccgagcc tgcaaaaacg tctgtttcag aaatatggca ttgataatcc ggatatgaac
     2941 aaactgcaat ttcatctgat gctggatgaa tttttctaat aattaattgg accgcggtcc
     3001 gcgcgttgtc cttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg
     3061 tatatccatc ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc
     3121 cttggtgtat ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg
     3181 ggtgttcctt cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc
     3241 tgcgaggctg gccgtaggcc ggcctcagcc tgccgccttg ggccgggtga tgtcgtactt
     3301 gcccgccgcg aactcggtta ccgtccagcc cagcgcgacc agctccggca acgcctcgcg
     3361 cacccgctgg cggcgcttgc gcatggtcga accactggcc tctgacggcc agacatagcc
     3421 gcacaaggta tctatggaag ccttgccggt tttgccgggg tcgatccagc cacacagccg
     3481 ctggtgcagc aggcgggcgg tttcgctgtc cagcgcccgc acctcgtcca tgctgatgcg
     3541 cacatgctgg ccgccaccca tgacggcctg cgcgatcaag gggttcaggg ccacgtacag
     3601 gcgcccgtcc gcctcgtcgc tggcgtactc cgacagcagc cgaaacccct gccgcttgcg
     3661 gccattctgg gcgatgatgg ataccttcca aaggcgctcg atgcagtcct gtatgtgctt
     3721 gagcgcccca ccactatcga cctctgcccc gatttccttt gccagcgccc gatagctacc
     3781 tttgaccacc atggcatcag cggtgacggc ctcccacttg ggttccagga acagccggag
     3841 ctgccgtccg ccttcggtct tgggttccgg gccaagcact aggccattag gcccagccat
     3901 ggccaccagc ccttgcagga tgcgcagatc atcagcgccc agcggctccg ggccgctgaa
     3961 ctcgatccgc ttgccgtcgc cgtagtcata cgtcacgtcc agcttgctgc gcttgcgctc
     4021 gccccgcttg agggcacgga acaggccggg ggccagacag tgcgccgggt cgtgccggac
     4081 gtggctgagg ctgtgcttgt tcttaggctt caccacgggg cacccccttg ctcttgcgct
     4141 gcctctccag cacggcgggc ttgagcaccc cgccgtcatg ccgcctgaac caccgatcag
     4201 cgaacggtgc gccatagttg gccttgctca caccgaagcg gacgaagaac cggcgctggt
     4261 cgtcgtccac accccattcc tcggcctcgg cgctggtcat gctcgacagg taggactgcc
     4321 agcggatgtt atcgaccagt accgagctgc cccggctggc ctgctgctgg tcgcctgcgc
     4381 ccatcatggc cgcgcccttg ctggcatggt gcaggaacac gatagagcac ccggtatcgg
     4441 cggcgatggc ctccatgcga ccgatgacct gggccatggg gccgctggcg ttttcttcct
     4501 cgatgtggaa ccggcgcagc gtgtccagca ccatcaggcg gcggccctcg gcggcgcgct
     4561 tgaggccgtc gaaccactcc ggggccatga tgttgggcag gctgccgatc agcggctgga
     4621 tcagcaggcc gtcagccacg gcttgccgtt cctcggcgct gaggtgcgcc ccaagggcgt
     4681 gcaggcggtg atgaatggcg gtgggcgggt cttcggcggg caggtagatc accgggccgg
     4741 tgggcagttc gcccacctcc agcagatccg gcccgcctgc aatctgtgcg gccagttgca
     4801 gggccagcat ggatttaccg gcaccaccgg gcgacaccag cgccccgacc gtaccggcca
     4861 ccatgttggg caaaacgtag tccagcggtg gcggcgctgc tgcgaacgcc tccagaatat
     4921 tgataggctt atgggtagcc attgattgcc tcctttgcag gcagttggtg gttaggcgct
     4981 ggcggggtca ctacccccgc cctgcgccgc tctgagttct tccaggcact cgcgcagcgc
     5041 ctcgtattcg tcgtcggtca gccagaactt gcgctgacgc atccctttgg ccttcatgcg
     5101 ctcggcatat cgcgcttggc gtacagcgtc agggctggcc agcaggtcgc cggtctgctt
     5161 gtccttttgg tctttcatat cagtcaccga gaaacttgcc ggggccgaaa ggcttgtctt
     5221 cgcggaacaa ggacaaggtg cagccgtcaa ggttaaggct ggccatatca gcgactgaaa
     5281 agcggccagc ctcggccttg tttgacgtat aaccaaagcc accgggcaac caatagccct
     5341 tgtcactttt gatcaggtag accgaccctg aagcgctttt ttcgtattcc ataaaacccc
     5401 cttctgtgcg tgagtactca tagtataaca ggcgtgagta ccaacgcaag cactacatgc
     5461 tgaaatctgg cccgcccctg tccatgcctc gctggcgggg tgccggtgcc cgtgccagct
     5521 cggcccgcgc aagctggacg ctgggcagac ccatgacctt gctgacggtg cgctcgatgt
     5581 aatccgcttc gtggccgggc ttgcgctctg ccagcgctgg gctggcctcg gccatggcct
     5641 tgccgatttc ctcggcactg cggccccggc tggccagctt ctgcgcggcg ataaagtcgc
     5701 acttgctgag gtcatcaccg aagcgcttga ccagcccggc catctcgctg cggtactcgt
     5761 ccagcgccgt gcgccggtgg cggctaagct gccgctcggg cagttcgagg ctggccagcc
     5821 tgcgggcctt ctcctgctgc cgctgggcct gctcgatctg ctggccagcc tgctgcacca
     5881 gcgccgggcc agcggtggcg gtcttgccct tggattcacg cagcagcacc cacggctgat
     5941 aaccggcgcg ggtggtgtgc ttgtccttgc ggttggtgaa gcccgccaag cggccatagt
     6001 ggcggctgtc ggcgctggcc gggtcggcgt cgtactcgct ggccagcgtc cgggcaatct
     6061 gcccccgaag ttcaccgcct gcggcgtcgg ccaccttgac ccatgcctga tagttcttcg
     6121 ggctggtttc cactaccagg gcaggctccc ggccctcggc tttcatgtca tccaggtcaa
     6181 actcgctgag gtcgtccacc agcaccagac catgccgctc ctgctcggcg ggcctgatat
     6241 acacgtcatt gccctgggca ttcatccgct tgagccatgg cgtgttctgg agcacttcgg
     6301 cggctgacca ttcccggttc atcatctggc cggtggtggc gtccctgacg ccgatatcga
     6361 agcgctcaca gcccatggcc ttgagctgtc ggcctatggc ctgcaaagtc ctgtcgttct
     6421 tcatcgggcc accaagcgat tcccacacat tatacgagcc ggaagcataa agtgtagcta
     6481 gatccgaagg atgagccggg ctgaatgatc gaccgagaca ggccctgcgg ggctgcacac
     6541 gcgcccccac ccttcgggta gggggaaagg ccgctaaagc ggctaaaagc gctccagcgt
     6601 atttctgcgg ggtttggtgt ggggtttagc gggctttgcc cgcctttccc cctgccgcgc
     6661 agcggtgggg cggtgtgtag cctagcgcag cgaatagacc agctatccgg cctctggccg
     6721 ggcatattgg gcaagggcag cagcgcccca caagggcgct gataaccgcg cctagtggat
     6781 tattcttaga taatcatgga tggatttttc caacaccccg ccagcccccg cccctgctgg
     6841 gtttgcaggt ttgggggcgt gacagttatt gcaggggttc gtgacagtta ttgcaggggg
     6901 gcgtgacagt tattgcaggg gttcgtgaca gttagggcgc gcccagctgt ctagggcggc
     6961 ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag
     7021 tctttcgact gagcctttcg ttttatttga tgcctttaat taaacgttcg taatcaagcc
     7081 acttcctttt tgcattgacg cagggtgtcg gaaggcaact cgccgaacgc gctcctatag
     7141 ttttcagcga agcgtcccaa atgtaagaag ccgtagtcta gggctatctc agttatacta
     7201 cgcacattgg cactgggatc gttcaagcag gcgcggatgc tttcgagctt gcggttgcgg
     7261 atgtagttct tcggcgtggt gccggcgtgc ttctcgaaca aattgtagag cgagcgtgga
     7321 ctcatcatcg ccagctccgc taaccgctca aggctgatat tccgtttgag attctcctca
     7381 atgaattgaa cgactcgctc gaaagacggg ttacctttgc tgaaaatttc acggctgaca
     7441 ttgctgccca gcatttcgag cagcttggaa gcgatgatcc ccgcatagtg ctcttggacc
     7501 cgaggcatcg actttgtatg ttccgcttcg tcacaaacta acccgagtag attgataaag
     7561 ccatcgagtt gctggagatt gtgtcgcgcg gcgaaacgga taccctccct cggcttgtgc
     7621 caattgttgt cactgcacgc ccgatcaagg accactgagg gcaatttaac gataaatttc
     7681 tcgcaatctt ctgaataggt caggtcggct tggtcatccg gattgagcag caatagttcg
     7741 cccggcgcaa aatagtgctc ctggccatgg ccacgccaca ggcaatggcc tttgagtatt
     7801 atttgcagat gataacaggt ctctaatcca ggcgagatta ccctcacgct accgccgtag
     7861 ctgattcgac acagatcgag gcatccgaag attctgtggt gcagcctgcc tgccgggcgc
     7921 ccgcccttgg gcaggcgaat agagtgcgta ccgacatact ggttaacata atcggagact
     7981 gcatagggct cggcgtggac gaagatctga cttttctcgt tcaataagca aaaatccata
     8041 gttcacggtt ctcttatttt aatgtgggct gcttggtgtg atgtagaaag gcgccaagtc
     8101 gatgaaaatg catctcgacg tgatgcgtat acgggttacc cccattgcca cgttgcgcca
     8161 tcctttttgc aatcagtgac cacttttcca agcaaaaata acgccaagca gaacgaagac
     8221 gttcttttta agaagcgaga acaccagaag ttcgtgctgt cggggcatgg ggcgacgaat
     8281 tggcggataa aggggatctg ctggatatta cggccttttt aaagaccgta aagaaaaata
     8341 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgt
     8401 aattacgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
     8461 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg
     8521 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg
     8581 cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga
     8641 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca
     8701 ccatgcatgg gaattagctt gatctgacca acgaccggta gcggagctat ccaacggcgg
     8761 tataccagga aaacacacag caggtacatc agaacagtac catgactgaa gaacaaatag
     8821 ttttttcctg atccataaag cagaacggcc tgctccatga caaatctggc tccccaacta
     8881 atgccccatg cagccagcat aaccagcata aagtgcagtg tccggtttga tagggataag
     8941 tccagccttg caagaagcgg atacaggagt gcaaaaaatg gctatctcta gtaaggccta
     9001 ccccttaggc tttatgcaac agaaacaata ataatggagt catgaccatg cctaggccgc
     9061 gggccgcgcg aattcagaag gagaatatac catgaaccaa atcgtgaagt tcactgacga
     9121 ctctggcctg gcggttcaag ttactccaga cgatgttcgc cgttatatct gtgagaacgc
     9181 tactgaaaaa gaggtgggcc tctttctgca actctgtcag actcaacgtc tgaatccgtt
     9241 tgtgaaagac gcttacctgg tgaaatacgg cggtgctcca gcttctatga ttacttccta
     9301 tcaagttttt aaccgtcgcg cgtgtcgtga tgctaactat gatggtatca aatctggtgt
     9361 ggttgttctg cgtgacggtg atgttgtgca taaacgtggt gctgcgtgct acaaaaaggc
     9421 gggtgaggag ctcatcggtg gttgggcgga agttcgcttt aaggatggcc gcgagactgc
     9481 gtatgctgag gtggcgctcg acgactattc caccggcaaa tctaattggg cgaaaatgcc
     9541 gggtgttatg atcgaaaaat gcgcgaaggc tgctgcttgg cgcctcgcgt tcccggacac
     9601 ttttcagggc atgtacgctg cggaggaaat ggatcaagcg caacagccag aacaggtgcg
     9661 cgctcaggcg gagcaaccag tggatctcca gccaatccgc gaactcttca agccatattg
     9721 cgaacacttc ggcatcactc cggctgaggg tatgactgct gtttgtggtg cggtgggcgc
     9781 tgaaggcatg cactctatga ccgagcagca agctcgccgt gctcgcgctt ggatggagga
     9841 agaaatggct gcgccagctg tggaagcgga gtatgaggtt gttgacgagg gcgaggtgtt
     9901 ttaag
//
