LOCUS       sequence-267393-        8861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Piggybac vector for CRISPR/SpCas9 applications (nuclease, base 
            editing, or epigenetic modifications) coexpressed with tdTomato-NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8861)
  TITLE     Madison Lab plasmids
REFERENCE   2  (bases 1 to 8861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BII-gR-PnTW
FEATURES             Location/Qualifiers
     source          1..8861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..17
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6..28
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     25..41
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     25..41
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   532..566
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(835..851)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(835..851)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        1107..1355
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1107..1127
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1278..1297
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        1376..1451
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             2783..2836
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2843..2869
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2843..2869
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2876..4303
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             4307..4327
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    4364..4952
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4417..4437)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4835..4846)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5032..5166
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5056..5075)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5110..5129
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   5945..6007
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6137..6154)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6137..6153)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6146..6168)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6295..6750
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6382..6401)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6592..6613
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6776..6880
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6881..6949,6950..7741)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7099..7118)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7912..8500
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8401..8420
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8654..8671
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8788..8809
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8824..8829,8830..8847,8848..8854)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
       61 cccgccgggt aactcacggg gtatccatgt ccatttctgc ggcatccagc caggataccc
      121 gtcctcgctg acgtaatatc ccagcgccgc accgctgtca ttaatctgca caccggcacg
      181 gcagttccgg ctgtcgccgg tattgttcgg gttgctgatg cgcttcgggc tgaccatccg
      241 gaactgtgtc cggaaaagcc gcgacgaact ggtatcccag gtggcctgaa cgaacagttc
      301 accgttaaag gcgtgcatgg ccacaccttc ccgaatcatc atggtaaacg tgcgttttcg
      361 ctcaacgtca atgcagcagc agtcatcctc ggcaaactct ttccatgccg cttcaacctc
      421 gcgggaaaag gcacgggctt cttcctcccc gatgcccaga tagcgccagc ttgggcgatg
      481 actgagccgg aaaaaagacc cgacgatatg atcctgatgc agctagatta accctagaaa
      541 gatagtctgc gtaaaattga cgcatgcatt cttgaaatat tgctctctct ttctaaatag
      601 cgcgaatccg tcgctgtgca tttaggacat ctcagtcgcc gcttggagct cccgtgaggc
      661 gtgcttgtca atgcggtaag tgtcactgat tttgaactat aacgaccgcg tgagtcaaaa
      721 tgacgcatga ttatctttta cgtgactttt aagatttaac tcatacgata attatattgt
      781 tatttcatgt tctacttacg tgataactta ttatatatat attttcttgt tatagatcca
      841 ctagttctag agggacagcc cccccccaaa gcccccaggg atgtaattac gtccctcccc
      901 cgctaggggg cagcagcgag ccgcccgggg ctccgctccg gtccggcgct ccccccgcat
      961 ccccgagccg gcagcgtgcg gggacagccc gggcacgggg aaggtggcac gggatcgctt
     1021 tcctctgaac gcttctcgct gctctttgag cctgcagaca cctgggggga tacggggaaa
     1081 aggcctccag ggccgccccc ttcaccgagg gcctatttcc catgattcct tcatatttgc
     1141 atatacgata caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga
     1201 tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa
     1261 aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct
     1321 tggctttata tatcttgtgg aaaggacgaa acaccggaga cgctgacccg tctctgtttt
     1381 agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac
     1441 cgagtcggtg cttttttgtt tttgcctcca aggcctgcag gagtcaatgg gaaaaaccca
     1501 ttggagccaa gtacactgac tcaataggga ctttccattg ggttttgccc agtacataag
     1561 gtcaataggg ggtgagtcaa caggaaagtc ccattggagc caagtacatt gagtcaatag
     1621 ggactttcca atgggttttg cccagtacat aaggtcaatg ggaggtaagc caatgggttt
     1681 ttcccattac tgacatgtat actgagtcat tagggacttt ccaatgggtt ttgcccagta
     1741 cataaggtca ataggggtga atcaacagga aagtcccatt ggagccaagt acactgagtc
     1801 aatagggact ttccattggg ttttgcccag tacaaaaggt caataggggg tgagtcaatg
     1861 ggtttttccc attattggca catacataag gtcaataggg gtgactagtg gagaagagca
     1921 tgcttgaggg ctgagtgccc ctcagtgggc agagagcaca tggcccacag tccctgagaa
     1981 gttgggggga ggggtgggca attgaactgg tgcctagaga aggtggggct tgggtaaact
     2041 gggaaagtga tgtggtgtac tggctccacc tttttcccca gggtggggga gaaccatata
     2101 taagtgcagt agtctctgtg aacattcggt ttagtgaacc tgcaggtcgg ccgccacgac
     2161 cggtgccacc atgactgaat acaaaccaac tgttcgcctg gcaactcgtg atgatgttcc
     2221 acgtgcagtt cgcaccctgg ctgctgcatt tgctgactac cctgcaaccc gtcacactgt
     2281 ggacccagac cgccacattg aacgtgtgac tgaactgcag gagctgttcc tgacccgtgt
     2341 gggcctggac attggcaaag tgtgggtggc agatgatggt gctgctgtgg cagtgtggac
     2401 cacccctgaa tctgttgaag ctggtgcagt gtttgctgag attggcccac gcatggcaga
     2461 actgtctggc agccgcctgg cagcacaaca gcagatggaa ggtctgctgg caccacaccg
     2521 cccaaaagaa cctgcttggt tcctggcaac tgtgggtgtg agccctgacc accagggtaa
     2581 gggcctgggc tctgcagtgg tgctgcctgg tgtggaagca gctgaacgtg caggtgtgcc
     2641 tgctttcctg gagacctcag ctccacgcaa cctgcctttc tatgaacgcc tgggcttcac
     2701 tgtgactgct gatgtggaag tgccagaagg cccacgcact tggtgcatga ctcgcaaacc
     2761 aggtgctagc cttggctccg gcgagggcag gggaagtctt ctaacatgcg gggacgtgga
     2821 ggaaaatccc ggcccactcg gctaccccta cgacgtgccc gactacgcca gcggcatggt
     2881 gagcaagggc gaggaggtca tcaaagagtt catgcgcttc aaggtgcgca tggagggctc
     2941 catgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     3001 ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct
     3061 gtccccccag ttcatgtacg gctccaaggc gtacgtgaag caccccgccg acatccccga
     3121 ttacaagaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     3181 cggcggtctg gtgaccgtga cccaggactc ctccctgcag gacggcacgc tgatctacaa
     3241 ggtgaagatg cgcggcacca acttcccccc cgacggcccc gtaatgcaga agaagaccat
     3301 gggctgggag gcctccaccg agcgcctgta cccccgcgac ggcgtgctga agggcgagat
     3361 ccaccaggcc ctgaagctga aggacggcgg ccactacctg gtggagttca agaccatcta
     3421 catggccaag aagcccgtgc aactgcccgg ctactactac gtggacacca agctggacat
     3481 cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgctccg agggccgcca
     3541 ccacctgttc ctggggcatg gcaccggcag caccggcagc ggcagctccg gcaccgcctc
     3601 ctccgaggac aacaacatgg ccgtcatcaa agagttcatg cgcttcaagg tgcgcatgga
     3661 gggctccatg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     3721 gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga
     3781 catcctgtcc ccccagttca tgtacggctc caaggcgtac gtgaagcacc ccgccgacat
     3841 ccccgattac aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     3901 cgaggacggc ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat
     3961 ctacaaggtg aagatgcgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa
     4021 gaccatgggc tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg
     4081 cgagatccac caggccctga agctgaagga cggcggccac tacctggtgg agttcaagac
     4141 catctacatg gccaagaagc ccgtgcaact gcccggctac tactacgtgg acaccaagct
     4201 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc gctccgaggg
     4261 ccgccaccac ctgttcctgt acggcatgga cgagctgtac aagggcccca agaagaagcg
     4321 caaggtgggc gcctaagtac aagtaagagt tcgcgtctgg aacaatcaac ctctggatta
     4381 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     4441 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     4501 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     4561 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     4621 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     4681 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     4741 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     4801 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     4861 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     4921 gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattctggct aagcacttcg
     4981 tggccgtcga tcgtttaaag ggaggtagtg agtcgagata tcgatactag tgatccagac
     5041 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     5101 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     5161 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     5221 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcctctagag
     5281 ggacagcccc cccccaaagc ccccagggat gtaattacgt ccctcccccg ctagggggca
     5341 gcagcgagcc gcccggggct ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc
     5401 agcgtgcggg gacagcccgg gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc
     5461 ttctcgctgc tctttgagcc tgcagacacc tggggggata cggggaaaaa gccataccaa
     5521 tggagctcac ggggacagcc cccccccaaa gcccccaggg atgtaattac gtccctcccc
     5581 cgctaggggg cagcagcgag ccgcccgggg ctccgctccg gtccggcgct ccccccgcat
     5641 ccccgagccg gcagcgtgcg gggacagccc gggcacgggg aaggtggcac gggatcgctt
     5701 tcctctgaac gcttctcgct gctctttgag cctgcagaca cctgggggga tacggggaaa
     5761 aagaattcga taaaagtttt gttactttat agaagaaatt ttgagttttt gtttttttta
     5821 ataaataaat aaacataaat aaattgtttg ttgaatttat tattagtatg taagtgtaaa
     5881 tataataaaa cttaatatct attcaaatta ataaataaac ctcgatatac agaccgataa
     5941 aacacatgcg tcaattttac acatgattat ctttaacgta cgtcacaata tgattatctt
     6001 tctagggtta atctagctgc gtgttctgca gcgtgtcgag catcttcatc tgctccatca
     6061 cgctgtaaaa cacatttgca ccgcgagtct gcccgtcctc cacgggttca aaaacgtgaa
     6121 tgaacgaggc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     6181 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     6241 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     6301 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     6361 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     6421 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     6481 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     6541 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     6601 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     6661 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     6721 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     6781 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     6841 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     6901 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     6961 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     7021 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     7081 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     7141 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     7201 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     7261 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     7321 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     7381 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     7441 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     7501 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     7561 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     7621 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     7681 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     7741 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     7801 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     7861 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     7921 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     7981 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     8041 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     8101 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     8161 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     8221 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     8281 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     8341 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8401 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8461 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     8521 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     8581 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     8641 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     8701 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     8761 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     8821 ggctttacac tttatgcttc cggctcgtat gttgtgtgga a
//
