LOCUS       sequence-267416-       10070 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human VprBP RARA mutant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10070)
  AUTHORS   Nakagawa T, Lv L, Nakagawa M, Yu Y, Yu C, D'Alessio AC, Nakayama K, 
            Fan HY, Chen X, Xiong Y
  TITLE     CRL4(VprBP) E3 Ligase Promotes Monoubiquitylation and Chromatin 
            Binding of TET Dioxygenases.
  JOURNAL   Mol Cell. 2015 Jan 22;57(2):247-60. doi: 
            10.1016/j.molcel.2014.12.002. Epub 2014 Dec 31.
  PUBMED    25557551
REFERENCE   2  (bases 1 to 10070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.12.002"; journalName: "Mol Cell"; date: 
            "2015-01-22- 22"; volume: "57"; issue: "2"; pages: "247-60"
COMMENT     Sequence Label: pcDNA3-HA-VprBP (RARA)
FEATURES             Location/Qualifiers
     source          1..10070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             685..711
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    724..748
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(2004..2015)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5331..5355)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(5389..5407)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5390..5407)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5427..5444)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5433..5657
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5703..6131
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5790..5809)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6000..6021
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6140..6160)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6145..6474
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6325..6460
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6387..6406
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6541..7335
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6595..6614)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7205..7224
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7509..7630
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7546..7565)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7600..7619
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7679..7695)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7679..7695)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7692..7714)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7703..7719
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7727..7733,7734..7751,7752..7757))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7772..7793
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7910..7927)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8081..8669)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8161..8180)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8840..9631,9632..9700))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9463..9482
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9701..9805)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9880..9899)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac catgtaccca tacgacgtcc cagactacgc tgaattcgat
      721 atcacaagtt tgtacaaaaa agcaggctct ttaaaggaac caattcagtc gactggatcc
      781 ggtaccgaat tcatgactac agtagtggta catgtggact ccaaagctga gctcactacc
      841 ctgctggagc agtgggaaaa ggaacatggc agtgggcagg acatggtacc tatccttacc
      901 aggatgtctc aattgattga aaaagaaact gaagagtatc gtaaagggga tccagaccca
      961 tttgatgatc gacatcctgg tcgagctgat ccagagtgta tgctgggcca cttgctgaga
     1021 atactcttca agaatgatga tttcatgaat gcactggtga atgcatatgt gatgacaagc
     1081 cgagagcccc ctttaaacac tgcagcttgc agactcctat tagacatcat gccagggctg
     1141 gaaactgctg tcgtctttca agaaaaggag ggaattgtcg agaatctttt caaatgggcc
     1201 cgagaggccg atcaaccatt gaggacatat tctactggac tgttaggagg tgctatggaa
     1261 aatcaagaca ttgctgccaa ctatagagat gaaaattcac agctggtggc aatagtgctt
     1321 cgaagactga gggagctaca gctacaggaa gtggctttgc ggcaggaaaa caagcgtccc
     1381 agtccacgga agctctcttc tgaacccctt ttgcctctgg atgaggaggc tgtggatatg
     1441 gactatggtg acatggctgt agatgtagtg gatggagacc aagaggaagc ttctggagac
     1501 atggagatct cctttcatct tgattcaggc cacaagacta gtagcagagt gaactcaaca
     1561 accaaacctg aggatggagg attaaagaaa aacaagtcag caaaacaggg tgacagagag
     1621 aactttagga aagccaagca aaagttgggt ttctcatctt ctgatccaga tcgcatgttt
     1681 gttgagctgt ctaatagcag ttggtcagaa atgtctccct gggtgattgg caccaattat
     1741 accctttatc ctatgactcc tgctatcgag cagcgactca ttctccaata tttgacccct
     1801 ctaggagaat atcaggagcc acttcccata ttcatgcaac ttggatcacg ggagctgatg
     1861 atgttctata ttgacctgaa gcaaactaat gatgtcctgc ttacatttga ggcactaaag
     1921 cacctagcat ctctcctgct ccataacaaa tttgccacag aatttgttgc gcatggtgga
     1981 gtacagaaat tactggaaat acctcgtcct tctatggctg caaccggtgt gtctatgtgt
     2041 ttgtattacc tatcctacaa tcaggatgcc atggaaagag tttgcatgca tccccacaat
     2101 gttctgtctg atgtggtgaa ctataccctg tggttaatgg agtgttctca tgcttcagga
     2161 tgctgccatg ctaccatgtt tttttcaatt tgcttctcat ttcgggccgt cttggagctc
     2221 tttgaccgct atgatggtct tcgtcgtctg gtgaacttga tcagtacttt ggagattcta
     2281 aatttggaag atcagggtgc acttctgagt gatgatgaaa tatttgctag ccgccaaact
     2341 gggaaacata cctgcatggc cttgcgcaaa tactttgagg ctcacctggc cattaaattg
     2401 gaacaagtga agcagtcact tcagaggact gagggtggca ttcttgtcca cccacaaccc
     2461 ccgtacaagg catgctcata tactcatgaa cagattgtgg aaatgatgga atttttgata
     2521 gaatatggcc cagcgcagct atattgggaa ccagctgaag ttttcctcaa actttcttgt
     2581 gtgcaactct tgttgcagct tatttctatt gcctgcaatt ggaagaccta ttatgcaagg
     2641 aatgacactg tgcgctttgc tttggatgtc ctggctattc ttactgtggt gccaaaaatc
     2701 cagctccagt tggcagaatc agtggacgtg ttggatgagg ctggatctac agtctctact
     2761 gtaggtatca gcattatttt gggagtggct gagggtgagt tcttcatcca tgatgctgaa
     2821 attcagaagt cagcacttca gattatcatc aattgtgtgt gtggcccaga taaccgaata
     2881 tccagtattg gtaaatttat ctctggtact cctcggagaa agctgcctca gaaccctaaa
     2941 agcagtgagc acaccctggc caagatgtgg aatgtggttc agtccaacaa cggcatcaag
     3001 gtgctcctgt ccttactgtc cattaagatg cccatcacag atgcagacca aatccgggcc
     3061 ctggcctgca aagccctagt gggcctgtct cgcagtagca ctgtccggca gatcatcagt
     3121 aaactgcccc ttttcagcag ctgccagatc cagcagctga tgaaggagcc tgtgctgcag
     3181 gacaagcgca gtgaccatgt caagttctgc aagtatgctg ctgaactcat tgaacgggtg
     3241 tcaggaaaac cacttctcat tggcactgat gtttccctag cacgactgca gaaagcagat
     3301 gttgttgccc agtcaaggat ctccttccct gagaaagagc tgcttttgtt gatacgaaac
     3361 catcttattt ctaaagggct tggagaaaca gcaaccgtgc tgacaaaaga ggctgacctg
     3421 cccatgactg ctgcctccca ttcttctgcc tttaccccag tcactgctgc tgcttctcct
     3481 gtctctctac cccgaacccc tcgtatcgct aatggcattg caactcgtct gggcagccat
     3541 gctgctgtgg gtgcctctgc gccttctgcc cctactgctc atcctcagcc acggcccccc
     3601 cagggtccgc tagctctgcc cggcccatct tatgcaggca actccccttt gattggtaga
     3661 atcagtttta tcagagagag gccatcaccc tgcaatggca ggaaaatcag agtgttgcgg
     3721 cagaagtcgg accatggtgc ctacagccaa agcccagcca taaaaaaaca gctggacaga
     3781 catcttcctt ccccacctac gctggacagt ataatcacag agtatcttag agaacaacat
     3841 gctcgctgca agaatccagt tgccacctgc ccacctttct ccctctttac tcctcaccaa
     3901 tgtcctgagc caaaacagag gcggcaagcg ccaataaact ttacgtcaag gctaaaccgc
     3961 agggcatcat ttccaaagta tggaggggtg gatggcggat gctttgatag gcaccttatc
     4021 tttagcagat tccgtcctat ttcagtgttc cgggaagcca atgaagatga gagtggcttc
     4081 acctgctgtg cattctcagc acgggagcgg ttcctgatgc ttggcacctg cacagggcag
     4141 ctgaagctct ataatgtgtt tagtggacag gaggaggcca gctataactg tcacaactca
     4201 gccatcacac atcttgaacc ttccagggat gggtccttgc tgctgacatc tgctacttgg
     4261 agccagcctt tgtctgcact ttggggaatg aagtcagtat ttgatatgaa gcattccttc
     4321 acagaagatc actatgttga gttcagtaag cactcccagg atcgggtcat cggcacaaaa
     4381 ggagacattg cccacattta tgatattcag actggcaaca agctgttgac tctgtttaac
     4441 ccagatcttg ccaacaacta caagaggaac tgtgccacct ttaatcctac agatgatctt
     4501 gtcttaaatg atggcgtcct ctgggacgtc gcctctgcac aggccatcca caagtttgac
     4561 aagttcaata tgaacatcag tggtgttttc catccaaatg gactggaggt gatcattaat
     4621 actgagattt gggacctcgc gacttttcat cttttgcata ctgttcccgc tctggatcag
     4681 tgtcgcgtgg tgttcaatca cacgggaaca gtgatgtatg gagctatgtt gcaggcagat
     4741 gatgaagatg acttaatgga agagaggatg aaaagcccct ttgggtcatc cttccgaaca
     4801 tttaatgcaa ctgactacaa acctatagca accattgatg tgaaacggaa catctttgac
     4861 ctgtgtacag acaccaaaga ctgctatctt gctgtcattg agaatcaagg cagcatggat
     4921 gccctgaaca tggacacagt atgcaggctg tatgaagtgg gcaggcagcg tctggcagag
     4981 gatgaggatg aagaggagga ccaggaagag gaagaacagg aggaagaaga tgatgatgaa
     5041 gatgatgatg acaccgatga tttagatgag cttgacactg accagttgct ggaggcggag
     5101 ttggaggagg acgacaataa tgagaacgca ggggaagatg gggacaatga cttctctccc
     5161 tctgatgagg agctagcaaa ccttctagag gagggagagg acggggagga tgaagactct
     5221 gatgcagatg aggaggtgga actgatcttg ggggacactg acagctctga caactctgat
     5281 ttggaagatg acatcatctt atctctgaat gagtgactcg agatatctag acccagcttt
     5341 cttgtacaaa gtggtgatat cctcgagcat gcatctagag ggccctattc tatagtgtca
     5401 cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     5461 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     5521 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     5581 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     5641 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     5701 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     5761 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     5821 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     5881 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     5941 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     6001 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     6061 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     6121 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     6181 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     6241 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     6301 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     6361 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     6421 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     6481 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc
     6541 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     6601 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     6661 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     6721 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     6781 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     6841 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     6901 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     6961 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     7021 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     7081 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     7141 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     7201 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     7261 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     7321 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     7381 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     7441 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     7501 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     7561 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     7621 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     7681 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     7741 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     7801 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     7861 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     7921 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     7981 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     8041 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     8101 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     8161 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     8221 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     8281 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8341 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8401 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     8461 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     8521 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     8581 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8641 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8701 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8761 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8821 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     8881 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     8941 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     9001 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9061 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9121 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9181 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9241 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9301 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9361 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9421 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9481 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9541 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9601 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9661 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9721 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9781 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     9841 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
     9901 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
     9961 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
    10021 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
//
