LOCUS       sequence-267417-        8529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses C-terminally strep tagged Kaposi's Sarcoma Associated 
            Herpesvirus (KSHV) ORF6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8529)
  AUTHORS   Davis ZH, Verschueren E, Jang GM, Kleffman K, Johnson JR, Park J, 
            Von Dollen J, Maher MC, Johnson T, Newton W, Jager S, Shales M, 
            Horner J, Hernandez RD, Krogan NJ, Glaunsinger BA
  TITLE     Global mapping of herpesvirus-host protein complexes reveals a 
            transcription strategy for late genes.
  JOURNAL   Mol Cell. 2015 Jan 22;57(2):349-60. doi: 
            10.1016/j.molcel.2014.11.026. Epub 2014 Dec 24.
  PUBMED    25544563
REFERENCE   2  (bases 1 to 8529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.11.026"; journalName: "Mol Cell"; date: 
            "2015-01-22- 22"; volume: "57"; issue: "2"; pages: "349-60"
COMMENT     Sequence Label: pCDNA4.TO-ORF6-2xCSTREP
FEATURES             Location/Qualifiers
     source          1..8529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4428..4451
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4488..4511
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(4537..4554)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4543..4767
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4813..5241
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4900..4919)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5110..5131
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5250..5270)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5255..5584
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5435..5570
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5497..5516
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5632..5679
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5698..6072
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6202..6323
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6239..6258)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6293..6312
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6372..6388)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6372..6388)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6385..6407)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6396..6412
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6420..6426,6427..6444,6445..6450))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6465..6486
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6603..6620)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6774..7362)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6854..6873)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7533..8324,8325..8393))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8156..8175
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8394..8498)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc catggcgcta aagggaccac
     1021 aaaccctcga ggaaaatatt gggtctgcgg cccccactgg tccctgcggg tacctctatg
     1081 cctatctgac acacaacttc cccatagggg aagcctccct gctgggcaat ggctacccgg
     1141 aggcaaaagt attttcacta cctcttttgc acgggctcac agtggaatcc gatttcccct
     1201 taaacgtaaa ggcggtgcac aagaaaatcg atgcaaccac agcttctgtg aaattaactt
     1261 cataccacag ggaggccatc gtctttcata atactcactt atttcagcca atctttcaag
     1321 gaaagggact ggaaaagtta tgtcgagaga gccgagagct gtttggattt tcaacgtttg
     1381 ttgagcagca acacaaaggg acgctctgga gcccagaggc atgccctcag ctaccctgcg
     1441 cgaatgagat ttttatggcg gtcatagtta cagagggatt caaggagaga ctgtacggcg
     1501 gcaaactggt gcccgtgccc tctcagacaa cgcccgtaca cattggggaa caccaggcgt
     1561 tcaagatacc cttgtatgac gaggatctgt ttggtccaag tcgcgcccaa gaactatgta
     1621 ggttttacaa ccccgatatc agtagatacc tacatgactc catattcact ggaatagcac
     1681 aggctctaag ggtaaaggac gttagcacgg tcatccaagc ctcagaaagg caatttgtgc
     1741 acgaccaata caagatacca aagctggtcc aagccaagga cttcccccag tgtgcttcca
     1801 ggggaaccga cgggtctacc ctaatggtga tagacagtct ggtggctgaa cttggtatga
     1861 gttatggtct gtcctttatt gagggacccc aggatagctg cgaggttcta aattatgaca
     1921 cgtggcccat ctttgaaaac tgcgagacgc cagatgcccg ccttcgtgca ctagaagttt
     1981 ggcacgcaga gcaggccttg catattggcg cccagctgtt tgcggccaac tctgtgctct
     2041 acctgaccag agtggcaaag ctgcctcaga ggaatcagag aggagacgcc aacatgtaca
     2101 actcattcta cctacagcat ggcctgggat acctctcaga ggcaacagta aaggaaaatg
     2161 gagcctctgc cttcaagggc gtgccagtgt ctgcactgga tgggtcatct tacaccctcc
     2221 agcacctggc ctacgcgtcc tctttctccc cacatctcct ggcaaggatg tgttactatc
     2281 tgcagttctt gccccaccat aaaaacacca acagtcagtc atacaatgtg gtggactacg
     2341 tgggcaccgc ggcacctagt caaatgtgtg acctgtgtca ggggcaatgt ccagctgtat
     2401 gcatcaacac gctgttttac aggatgaagg acaggttccc acctgttctg tcaaacgtta
     2461 agagagaccc atatgtgatc acgggcacag cgggaacgta caatgaccta gagattctcg
     2521 gaaactttgc caccttcagg gagagagagg aggaggggaa tcctgtggaa gatgctccaa
     2581 ggtatacgta ttggcaacta tgccagaata taaccgagaa gctagcgtcc atgggcatct
     2641 cggagggcgg cgatgcccta agaaccctca ttgtgaacat ccccagcttc gtcaaagtgt
     2701 tcaaggggat agacagcacg gtagaggcag agctcctaaa gtttattaac tgcatgatca
     2761 aaaacaatta caacttcaga gagaacatca aatccgtcca tcacatcctt cagtttgcat
     2821 gcaacgtata ctggcaggcg ccgtgcccgg tttttctgac cctttactac aagtcactgc
     2881 tgacggtcat acaggacata tgtctgacgt catgtatgat gtacgagcag gacaacccgg
     2941 ccgtgggaat tgtaccatcc gagtggctta aaatgcactt tcagacaatg tggaccaact
     3001 tcaagggtgc ctgcttcgac aaaggagcaa tcacgggcgg ggaactaaaa atagtccacc
     3061 agtccatgtt ctgtgacctc tttgacaccg acgctgccat aggagggatg tttgcacccg
     3121 ctcggatgca ggtcaggata gccagagcaa tgctcatggt cccaaaaacc ataaaaataa
     3181 aaaacaggat catcttttcc aactccaccg gagcagagtc gatccaggca ggttttatga
     3241 agccggccag ccaaagggat tcatacatcg tcggaggacc ctacatgaaa ttcctaaacg
     3301 ccctgcacaa aacacttttt ccttccacaa aaacttctgc cctgtacttg tggcataaga
     3361 ttggccagac cacaaaaaat cccatactac caggtgtctc gggggaacac ctaacggagt
     3421 tatgtaatta tgtaaaggca agtagccagg ctttcgaaga gataaatgtt ttggaccttg
     3481 tgccagacac cctgacctca tatgcgaaaa taaaactaaa cagttccatt ctccgggctt
     3541 gcggacggac acagttttat gcaactactc tctcttgcct tccgccagtg actcagctgg
     3601 ttccggccga ggagtacccc cacgtactgg ggccagtggg gttgtcatct ccagatgaat
     3661 acagggcaaa agtcgccggc aggtccgtaa ccattgtaca gtcaacactg aagcaagctg
     3721 tttccaccaa cggacgactc cggcctatca ttaccgtgcc actggtggtc aacaaatata
     3781 cagggagcaa cgggaacaca aacgtctttc actgtgcaaa cctgggatac ttctcgggga
     3841 gaggggtgga cagaaatctc aggccagaaa gcgtcccctt taaaaagaat aatgtcagct
     3901 ctatgctaag aaaacgccac gtgattatga cccccctggt agacaggctg gtaaagagaa
     3961 tagttggcat caactctggg gaattcgagg cagaagcggt taagagaagt gtgcagaatg
     4021 tcctggaaga cagagataac ccaaacctgc cgaagacagt tgtattagag ttggttaagc
     4081 acctagggag ctcctgtgca agtctcacag aggaggacgt gatttactac ctgggccctt
     4141 atgccgtact tggggacgag gtcctgtcat tactgagcac agtgggccag gcgggggtgc
     4201 catggacggc cgagggtgtg gcctcggtca tccaggacat aatagatgat tgcgagttac
     4261 agtttgtggg cccagaagag ccttgcctta tccaaggaca gtcggtagtg gaggagcttt
     4321 ttccgtcccc gggcgtccca agcctgacag tgggtaaaaa acgaaaaatc gcatccctgc
     4381 tctctgacct ggatttgtcg gggcggccgc tcgagggagg cggtggatgg agccaccccc
     4441 agttcgagaa gggcggcggc agcggcggcg gcagcggcgg cggcagctgg agccaccccc
     4501 agttcgagaa ggggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     4561 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     4621 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     4681 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     4741 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     4801 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4861 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     4921 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     4981 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     5041 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     5101 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     5161 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     5221 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     5281 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     5341 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5401 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     5461 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     5521 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     5581 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat
     5641 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
     5701 gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag
     5761 ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg
     5821 gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac
     5881 accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc
     5941 gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg
     6001 tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag
     6061 gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
     6121 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     6181 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     6241 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     6301 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     6361 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     6421 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     6481 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     6541 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     6601 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     6661 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     6721 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6781 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     6841 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     6901 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     6961 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7021 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7081 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7141 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7201 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7261 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7321 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7381 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     7441 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     7501 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     7561 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     7621 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     7681 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     7741 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     7801 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     7861 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     7921 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     7981 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     8041 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     8101 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     8161 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     8221 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     8281 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     8341 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     8401 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     8461 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     8521 cctgacgtc
//
