LOCUS       sequence-267433-       12623 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  inducible expression of Ncad and membrane-tethered GFP in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12623)
  AUTHORS   Toda S, Blauch LR, Tang SKY, Morsut L, Lim WA
  TITLE     Programming self-organizing multicellular structures with synthetic 
            cell-cell signaling.
  JOURNAL   Science. 2018 Jul 13;361(6398):156-162. doi: 
            10.1126/science.aat0271. Epub 2018 May 31.
  PUBMED    29853554
REFERENCE   2  (bases 1 to 12623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aat0271"; journalName: "Science"; date: 
            "2018-07-13- 13"; volume: "361"; issue: "6398"; pages: "156-162"
COMMENT     Sequence Label: pHR_TRE_Ncad-P2A-GFPlig
FEATURES             Location/Qualifiers
     source          1..12623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    5..23
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    41..59
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    77..95
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    113..131
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    149..167
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    185..203
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    221..239
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             3151..3207
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(3271..3273,3274..3276,3277..3987)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    4238..4826
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4291..4311)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4709..4720)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4829..4845)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4830..4846)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4936..5169
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5194..5212)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5195..5212)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5502..5521)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5735..5754)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5854..5876
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5914..5932)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6000..6104
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6105..6173,6174..6965)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6323..6342)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7136..7724
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7625..7644
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7878..7895
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7965..7985)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7970..8299
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8150..8285
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9433..9498
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9628..9648
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10073..10207
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10110..10129)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10164..10183
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10375..11008
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11055..11180
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11677..11910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12095..12139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12424..12541
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(12620..12623,1..364)
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
ORIGIN
        1 ttactcccta tcagtgatag agaacgtatg aagagtttac tccctatcag tgatagagaa
       61 cgtatgcaga ctttactccc tatcagtgat agagaacgta taaggagttt actccctatc
      121 agtgatagag aacgtatgac cagtttactc cctatcagtg atagagaacg tatctacagt
      181 ttactcccta tcagtgatag agaacgtata tccagtttac tccctatcag tgatagagaa
      241 cgtataagct ttgcttatgt aaaccagggc gcctataaaa gagtgctgat tttttgagta
      301 aacttcaatt ccacaacact tttgtcttat accaactttc cgtaccactt cctaccctcg
      361 taaagaattc ccgtatacac cggtgcaatt ctcacgcgtg ccgccaccat gtgccggata
      421 gcgggagcgc cgcggaccct gctgccgctt ctggcggcct tgcttcaggc gtctgtggag
      481 gcttctggtg aaattgcatt atgcaagact ggatttcctg aagatgttta cagcgcagtc
      541 ttaccgaagg atgtgcacga aggacagccc cttctcaatg tgaaattcag caactgcaat
      601 agaaaaagga aagttcagta tgaaagcagc gagccagcag atttcaaggt ggacgaggac
      661 ggcacggtgt atgctgtgag aagcttccct ctcactgcag agcaggcaaa gttcctgata
      721 tatgcccaag acaaagaaac ccaggaaaag tggcaggtag ctgtaaacct gagccgggag
      781 ccaaccctga ctgaggagcc tatgaaggaa ccacatgaaa ttgaagaaat agtattccct
      841 agacaacttg ccaagcacag tggagctcta caaaggcaga agagagactg ggtcatcccg
      901 ccaatcaact tgccagaaaa ctccagagga ccctttcctc aagagcttgt cagaatcagg
      961 tctgatagag ataaaaacct ttccctgaga tacagcgtca ctgggccagg agctgaccag
     1021 cctccaacgg gcatcttcat tatcaacccc atctcaggac agctgtcagt cacaaagcct
     1081 ctggatcgag agctgatagc ccggtttcac ttgagagcac atgcagtgga catcaatggc
     1141 aatcaagtgg agaaccccat tgacattgtc atcaatgtta ttgacatgaa tgataacaga
     1201 cctgagtttc tgcaccaggt ttggaatggg tctgttccag agggatcaaa gcctgggacg
     1261 tatgtgatga cggtcactgc cattgatgcg gatgatccaa atgccctgaa tggaatgctg
     1321 cggtacagga tcctgtccca ggcgcccagc acaccttcac ccaacatgtt tacaatcaac
     1381 aatgagactg gggacatcat cactgtggca gctggtctgg atcgagagaa agtgcaacag
     1441 tatacgttaa taattcaagc cacagacatg gaaggcaatc ccacttatgg cctttcaaac
     1501 acagccacag ccgtcatcac ggtgacagat gtcaatgaca atcctccaga gtttactgcc
     1561 atgactttct acggagaagt ccctgagaac agggtggacg tcattgtagc caacctaact
     1621 gtcacggaca aagatcagcc ccacacgccg gcctggaatg cggcatacag aatcagtggt
     1681 ggagacccta caggaaggtt tgccatcctg acagacccca acagcaatga tgggctagtc
     1741 acagtggtaa aaccaattga ctttgaaacg aataggatgt ttgtccttac tgttgctgca
     1801 gaaaaccaag tgccattagc taaaggcatt cagcacccac ctcagtcgac agccactgtg
     1861 tctgtgacag ttattgatgt caatgaaaat ccttattttg ccccaaatcc taaaatcatt
     1921 cgccaagagg aaggcctcca cgcaggtacc atgctgacca cgctcactgc tcaggacccc
     1981 gatcgatata tgcaacagaa tatcagatac acaaaattgt ctgatcctgc caactggctg
     2041 aaaatagacc ccgtgaatgg gcagatcact actattgccg ttttggacag agaatcgcca
     2101 aatgtaaaaa acaacatcta taatgctacc ttccttgctt ctgacaatgg aatcccgcct
     2161 atgagtggga caggaacact gcaaatctat ttacttgata tcaatgacaa cgcccctcag
     2221 gtgttacctc aagaggcaga gacctgtgaa actccagaac ccaactcaat taacatcaca
     2281 gcacttgatt atgacataga cccaaacgcc gggccattcg cgtttgatct tcccttatct
     2341 ccagtgacta ttaaaagaaa ctggaccatc aaccggctta atggtgattt tgctcagctc
     2401 aatttaaaga taaaattttt ggaagctggt atctatgaag ttcccatcat tatcacagat
     2461 tcagggaatc cccccaagtc caacatttcc atcctgcgtg tgaaagtttg ccagtgtgac
     2521 tccaatggag actgcacgga cgtggacagg atcgtgggtg cagggcttgg cacgggtgcc
     2581 atcatcgcta tccttctgtg tatcatcatc ctgctgatcc ttgttctcat gtttgtggta
     2641 tggatgaaac ggcgggataa agagcgccaa gccaagcagc ttttaattga cccagaagat
     2701 gatgtaagag ataatatatt gaaatatgat gaagaaggtg gaggagaaga agaccaggac
     2761 tatgacttga gccagctcca gcaaccagat actgtggagc ctgatgccat caagcccgtg
     2821 ggaatcagac ggctagacga gaggcctatc catgctgagc cacagtaccc agtccgatcc
     2881 gcagccccac accctgggga tattggggac ttcattaatg agggccttaa agctgctgac
     2941 aacgacccca cggcgccacc gtatgactcc ctcttagtct ttgactacga gggcagcggc
     3001 tccacggctg gctccttgag ctccctcaac tcctccagta gcggtgggga ccaggactat
     3061 gactacctga atgactgggg accccgcttc aagaaactgg cggacatgta cggcggtggt
     3121 gacgactcgc ggccggctag cggaagcggt gccacaaact tctctctgct aaagcaagca
     3181 ggtgatgttg aagaaaaccc cgggcctatg gagaccgaca ccctcttgct ctgggtcctg
     3241 ctgctgtggg ttccgggtag taccggagac atggtttcta agggtgaaga attgttcacc
     3301 ggagttgtgc ccatcctggt tgagctggac ggagacgtta acggacacaa gttctcagtg
     3361 agcggcgaag gagagggcga cgctacgtac ggtaaactga cgttgaagtt catctgtaca
     3421 acaggaaagc tgccagtgcc ctggcctaca ctcgtgacca ccctcaccta cggagtccag
     3481 tgcttctccc ggtaccccga ccacatgaaa cagcacgatt tcttcaagtc cgcgatgcct
     3541 gagggctatg ttcaggaacg caccatattc ttcaaggacg acggtaacta taaaaccagg
     3601 gctgaagtta agttcgaggg ggacacgttg gtaaatagaa ttgagttgaa aggaatcgat
     3661 ttcaaagaag acggcaatat tttgggccac aagctggagt acaactacaa cagccacaac
     3721 gtgtatatta tggctgataa gcagaagaac ggcatcaagg tgaacttcaa aatccgacac
     3781 aatatcgagg atggctctgt gcaactggcc gaccattacc agcaaaacac gcctatcggc
     3841 gacggtccgg ttttgctgcc agataaccac tacctctcca cgcaatcagc cctctccaag
     3901 gatcctaacg aaaaaagaga ccatatggtt ctgcttgaat tcgtgacggc tgctgggatc
     3961 accctgggaa tggatgagct gtataagaac gccgtgggcc aggatacgca ggaggtgatc
     4021 gtcgtgcccc acagcttgcc cttcaaggtc gtggttatta gtgctatcct ggcgttggtg
     4081 gtgctgacta ttatctccct tatcatactt atcatgctgt ggcaaaaaaa accccgataa
     4141 gcggccgcaa ctcccacctg caacatgcgt gactgactga ggccgcgact ctagagtcga
     4201 cctgcaggca tgcaagcttg atatcaagct tatcgataat caacctctgg attacaaaat
     4261 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     4321 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     4381 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     4441 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     4501 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     4561 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     4621 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     4681 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     4741 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     4801 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgaggga attaattcga
     4861 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     4921 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     4981 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     5041 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     5101 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     5161 tctctagcag catctagaat taattccgtg tattctatag tgtcacctaa atcgtatgtg
     5221 tatgatacat aaggttatgt attaattgta gccgcgttct aacgacaata tgtacaagcc
     5281 taattgtgta gcatctggct tactgaagca gaccctatca tctctctcgt aaactgccgt
     5341 cagagtcggt ttggttggac gaaccttctg agtttctggt aacgccgtcc cgcacccgga
     5401 aatggtcagc gaaccaatca gcagggtcat cgctagccag atcctctacg ccggacgcat
     5461 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
     5521 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
     5581 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct
     5641 tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
     5701 gcataaggga gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     5761 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     5821 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     5881 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     5941 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     6001 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     6061 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     6121 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     6181 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     6241 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     6301 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     6361 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     6421 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     6481 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     6541 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     6601 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     6661 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     6721 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     6781 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     6841 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     6901 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     6961 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     7021 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     7081 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7141 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     7201 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     7261 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     7321 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     7381 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     7441 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     7501 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     7561 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     7621 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     7681 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     7741 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     7801 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     7861 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     7921 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctgtggaatg tgtgtcagtt
     7981 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     8041 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     8101 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     8161 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     8221 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     8281 aggcctaggc ttttgcaaaa agcttggaca caagacaggc ttgcgagata tgtttgagaa
     8341 taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa
     8401 tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca
     8461 ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca
     8521 cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac
     8581 aatggaaagg cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg
     8641 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     8701 gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga
     8761 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
     8821 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
     8881 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     8941 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     9001 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     9061 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
     9121 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
     9181 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgcg
     9241 aaatcctcga cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
     9301 tatacgatac ggtgattggc taccgtggcg gcaactggat ttatgagtgg gccccggatc
     9361 tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt
     9421 taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta
     9481 attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat
     9541 gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct
     9601 actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag
     9661 gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt
     9721 gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg
     9781 gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt
     9841 tttcttactc cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt
     9901 acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg
     9961 actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
    10021 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
    10081 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    10141 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    10201 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
    10261 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
    10321 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag
    10381 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag
    10441 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
    10501 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
    10561 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
    10621 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
    10681 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
    10741 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
    10801 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
    10861 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
    10921 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
    10981 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
    11041 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
    11101 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
    11161 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
    11221 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
    11281 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
    11341 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
    11401 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
    11461 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
    11521 ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
    11581 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
    11641 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
    11701 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
    11761 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
    11821 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
    11881 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
    11941 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
    12001 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
    12061 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
    12121 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
    12181 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
    12241 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
    12301 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
    12361 cagatccatt cgattagtga acggatctcg acggtatcgc caaatggcag tattcatcca
    12421 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
    12481 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
    12541 tcgggtttat tacagggaca gcagagatcc agtttggatc gataagcttg atatcgaatt
    12601 cccgtggaat tatcacctcg agt
//
