LOCUS       sequence-267439-        8323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  S. pyogenes nicking sgRNA for mouse DNMT1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, 
            Chen PJ, Wilson C, Newby GA, Raguram A, Liu DR
  TITLE     Search-and-replace genome editing without double-strand breaks or 
            donor DNA.
  JOURNAL   Nature. 2019 Oct 21. pii: 10.1038/s41586-019-1711-4. doi: 
            10.1038/s41586-019-1711-4.
  PUBMED    31634902
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2019 Oct 21. pii: 10.1038/s41586-019-1711-4. doi: 
            10.1038/s41586-019-1711-4."
COMMENT     Sequence Label: pU6-sp-sgRNA-mDNMT1_nick-lenti
FEATURES             Location/Qualifiers
     source          1..8323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    573..806
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             991..1035
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1213..1461
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1213..1233
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1384..1403
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        1483..1558
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    1614..1731
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1870..3048
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2101..3039
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2996..3016
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3061..3660
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3061..3080)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3557..3577
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3676..4264
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3729..3749)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4147..4158)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4336..4569
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4647..4768
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4684..4703)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4738..4757
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4808..4943
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4963..4982)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4964..4982)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4992..5009)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4992..5008)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5001..5023)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5150..5605
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5237..5256)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5447..5468
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5631..5735
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5736..5804,5805..6596)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5954..5973)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6767..7355
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7256..7275
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7509..7526
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7643..7664
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7679..7684,7685..7702,7703..7709)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7717..7733
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7722..7744
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7741..7757
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7741..7757
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7776..7796
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7778..7796
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7824..8050
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8051..8231
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    join(8278..8323,1..80)
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
       61 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
      121 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
      181 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
      241 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
      301 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
      361 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
      421 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
      481 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
      541 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
      601 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
      661 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
      721 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
      781 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
      841 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
      901 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
      961 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     1021 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     1081 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     1141 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     1201 aggggaccca gagagggcct atttcccatg attccttcat atttgcatat acgatacaag
     1261 gctgttagag agataattag aattaatttg actgtaaaca caaagatatt agtacaaaat
     1321 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
     1381 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc
     1441 ttgtggaaag gacgaaacac cgccgcgcgc gcgaaaaagc cggttttaga gctagaaata
     1501 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
     1561 ttttaagctt ggcgtaacta gatcttgaga caaatggcag tattcatcca caattttaaa
     1621 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     1681 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     1741 tacagggaca gcagagatcc actttggcgc cggctcgagg gggcccgggt gcaaagatgg
     1801 ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt cttgaaagga
     1861 gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     1921 aagttggggg gaggggtcgg caattgatcc ggtgcctaga gaaggtggcg cggggtaaac
     1981 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     2041 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     2101 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg
     2161 ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga
     2221 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt
     2281 gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt
     2341 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt
     2401 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt
     2461 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg
     2521 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct
     2581 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg
     2641 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca
     2701 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg
     2761 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg
     2821 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt
     2881 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac
     2941 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag
     3001 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgacg tacggccacc
     3061 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     3121 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     3181 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     3241 atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac cacgccggag
     3301 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     3361 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     3421 cccgcgtggt tcctggccac cgtcggagtc tcgcccgacc accagggcaa gggtctgggc
     3481 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     3541 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     3601 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     3661 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     3721 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     3781 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     3841 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     3901 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     3961 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     4021 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     4081 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     4141 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     4201 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     4261 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     4321 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     4381 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     4441 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     4501 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     4561 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     4621 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     4681 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     4741 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     4801 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4861 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     4921 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     4981 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     5041 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     5101 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     5161 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     5221 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     5281 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     5341 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     5401 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     5461 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     5521 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     5581 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5641 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5701 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     5761 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5821 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5881 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5941 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     6001 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     6061 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     6121 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     6181 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     6241 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     6301 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     6361 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     6421 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     6481 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6541 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     6601 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     6661 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     6721 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6781 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6841 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6901 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6961 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7021 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7081 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     7141 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     7201 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     7261 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7321 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     7381 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     7441 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     7501 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     7561 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     7621 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     7681 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     7741 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     7801 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat
     7861 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
     7921 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
     7981 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
     8041 atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     8101 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     8161 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     8221 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     8281 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gag
//
