LOCUS       sequence-26751-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Nam HS, Benezra R
  TITLE     High levels of Id1 expression define B1 type adult neural stem 
            cells.
  JOURNAL   Cell Stem Cell. 2009 Nov 6. 5(5):515-26.
  PUBMED    19896442
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2009 Nov 6. 5(5):515-26."
COMMENT     Sequence Label: H2B-Orange
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     60..79
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        60..78
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             104..481
                     /codon_start=1
                     /gene="HIST1H2BB"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPSKSAPAPKKGSKKAITKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSSK"
     regulatory      494..503
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tnggnttagc agagctctct ggctactaga gaacccactg cttactggct tatcgaaatt
       61 aatacgactc actataggga gacccaagct tggtaccgcc accatgcctg aaccctctaa
      121 gtctgctcca gcccctaaaa agggttctaa gaaggctatc actaaggcgc agaagaagga
      181 tggtaagaag cgtaagcgca gccgcaagga gagctattct atctatgtgt acaaggttct
      241 gaagcaggtc caccccgaca ccggcatctc atccaaggcc atggggatca tgaactcctt
      301 cgtcaacgac atcttcgagc gcatcgcggg cgaggcttct cgcctggctc actacaataa
      361 gcgctcgacc atcacctcca gggagattca gacggctgtg cgcctgctgc tgcctgggga
      421 gctggctaag catgctgtgt ccgagggcac taaggcagtt accaagtaca ctagctctaa
      481 ggatccaccg gtcgccacca tggtgagcaa gggcgaggag aataacatgg ccatcatcaa
      541 ggagttcatg cgcttcaagg tgcgcatgga gggctccgtg aacggccacg agttcgagat
      601 cgagggcgag ggcgagggcc gcccctacga gggctttcag accgctaagc tgaaggtgac
      661 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca cctacggctc
      721 cnaggcctac gtgaagcacc ccgccgacat ccccgantac ttcagctgtc cttcccgang
      781 gcttcagtgg gagngcgtga tgancttcna gacgnggcgt gttgacgtga ccaggantct
      841 cctgcagg
//
