LOCUS       sequence-267512-        4855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast fluorescent (RFP) vector with PTEF promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Huh WK, Falvo JV, Gerke LC, Carroll AS, Howson RW, Weissman JS, 
            O'Shea EK
  TITLE     Global analysis of protein localization in budding yeast.
  JOURNAL   Nature. 2003 Oct 16;425(6959):686-91. doi: 10.1038/nature02026.
  PUBMED    14562095
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2003-10-16- 16"; volume: "425"; issue: "6959"; 
            pages: "686-91"
COMMENT     Sequence Label: pFA6a-mRFP1-kanMX6
FEATURES             Location/Qualifiers
     source          1..4855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     142..160
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(198..220)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     320..339
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        420..438
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     420..437
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             502..1179
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             502..1179
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(632..650)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     900..919
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1094..1117
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     terminator      1206..1393
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1261..1285)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            1468..2824
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        1468..1811
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1812..2621
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1879..1898)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      2627..2824
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(2928..2947)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2929..2947)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3034..3051)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3205..3793)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3285..3304)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3964..4755,4756..4824))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4587..4606
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(4825..4855,1..74))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
       61 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
      121 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
      181 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
      241 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
      301 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatggac
      361 atattgtcgt tagaacgcgg ctacaattaa tacataacct tatgtatcat acacatacga
      421 tttaggtgac actatagaac gcggccgcca gctgaagctt cgtacgctgc aggtcgacgg
      481 atccccgggt taattaactt catggcctcc tccgaggacg tcatcaagga gttcatgcgc
      541 ttcaaggtgc gcatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
      601 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc
      661 ctgcccttcg cctgggacat cctgtcccct cagttccagt acggctccaa ggcctacgtg
      721 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
      781 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
      841 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
      901 cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcggat gtaccccgag
      961 gacggcgccc tgaagggcga gatcaagatg aggctgaagc tgaaggacgg cggccactac
     1021 gacgccgagg tcaagaccac ctacatggcc aagaagcccg tgcagctgcc cggcgcctac
     1081 aagaccgaca tcaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag
     1141 tacgagcgcg ccgagggccg ccactccacc ggcgcctaag agctcggcgc gccacttcta
     1201 aataagcgaa tttcttatga tttatgattt ttattattaa ataagttata aaaaaaataa
     1261 gtgtatacaa attttaaagt gactcttagg ttttaaaacg aaaattctta ttcttgagta
     1321 actctttcct gtaggtcagg ttgctttctc aggtatagta tgaggtcgct cttattgacc
     1381 acacctctac cggcagatcc gctagggata acagggtaat atagatctgt ttagcttgcc
     1441 tcgtccccgc cgggtcaccc ggccagcgac atggaggccc agaataccct ccttgacagt
     1501 cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc cgtacattta gcccatacat
     1561 ccccatgtat aatcatttgc atccatacat tttgatggcc gcacggcgcg aagcaaaaat
     1621 tacggctcct cgctgcagac ctgcgagcag ggaaacgctc ccctcacaga cgcgttgaat
     1681 tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc actgaggttc
     1741 ttctttcata tacttccttt taaaatcttg ctaggataca gttctcacat cacatccgaa
     1801 cataaacaac catgggtaag gaaaagactc acgtttcgag gccgcgatta aattccaaca
     1861 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
     1921 caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
     1981 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
     2041 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
     2101 ctgcgatccc cggcaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
     2161 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
     2221 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
     2281 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
     2341 ggaaagaaat gcataagctt ttgccattct caccggattc agtcgtcact catggtgatt
     2401 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
     2461 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
     2521 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
     2581 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagtactg acaataaaaa
     2641 gattcttgtt ttcaagaact tgtcatttgt atagtttttt tatattgtag ttgttctatt
     2701 ttaatcaaat gttagcgtga tttatatttt ttttcgcctc gacatcatct gcccagatgc
     2761 gaagttaagt gcgcagaaag taatatcatg cgtcaatcgt atgtgaatgc tggtcgctat
     2821 actgctgtcg attcgatact aacgccgcca tccagtttaa acgagctcga attcatcgat
     2881 gatatcagat ccactagtgg cctatgcggc cgcggatctg ccggtctccc tatagtgagt
     2941 cgtattaatt tcgataagcc aggttaacct gcattaatga atcggccaac gcgcggggag
     3001 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     3061 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     3121 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     3181 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     3241 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3301 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     3361 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     3421 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     3481 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     3541 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3601 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     3661 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     3721 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     3781 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     3841 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3901 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     3961 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     4021 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     4081 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     4141 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     4201 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     4261 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     4321 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     4381 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     4441 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     4501 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     4561 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     4621 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     4681 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     4741 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     4801 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatt
//
