LOCUS       sequence-267517-        9565 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Membrane-anchored TF used for yeast directed evolution of TEV 
            protease.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9565)
  AUTHORS   Sanchez MI, Ting AY
  TITLE     Directed evolution improves the catalytic efficiency of TEV 
            protease.
  JOURNAL   Nat Methods. 2019 Dec 9. pii: 10.1038/s41592-019-0665-7. doi: 
            10.1038/s41592-019-0665-7.
  PUBMED    31819267
REFERENCE   2  (bases 1 to 9565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2019 Dec 9. pii: 10.1038/s41592-019-0665-7. doi: 
            10.1038/s41592-019-0665-7."
COMMENT     Sequence Label: 
            TDH3-STE2-Delta(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)LexAVP16-
            tCYC
FEATURES             Location/Qualifiers
     source          1..9565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             979..1002
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(1012..1014,1015..1017,1018..1728)
                     /codon_start=1
                     /product="blue variant of GFP"
                     /label=BFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1057..1078)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1318..1337)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1665..1686
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1753..2262
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     CDS             2896..2916
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQM"
     CDS             join(2926..3177,3178..3531)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     3469..3490
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
     CDS             3718..3951
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     terminator      3995..4244
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4011..4029)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        complement(4407..4425)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4446..4462)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4446..4462)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4459..4481)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4470..4486
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(4745..4762)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4916..5504)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4996..5015)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5683..6474,6475..6543))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6306..6325
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6544..6648)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(7027..8121)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFQNHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(8122..8527)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     8723..8745
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8737..8754
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8738..8754
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8761..8780
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8761..8779
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        8899..9565
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     9435..9458
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 actagtggaa cccccgggct gcaggaattc gatatcaagc ttatgtctga tgcggctcct
       61 tcattgagca atctatttta tgatccaacg tataatcctg gtcaaagcac cattaactac
      121 acttccatat atgggaatgg atctaccatc actttcgatg agttgcaagg tttagttaac
      181 agtactgtta ctcaggccat tatgtttggt gtcagatgtg gtgcagctgc tttgactttg
      241 attgtcatgt ggatgacatc gagaagcaga aaaacgccga ttttcattat caaccaagtt
      301 tcattgtttt taatcatttt gcattctgca ctctatttta aatatttact gtctaattac
      361 tcttcagtga cttacgctct caccggattt cctcagttca tcagtagagg tgacgttcat
      421 gtttatggtg ctacaaatat aattcaagtc cttcttgtgg cttctattga gacttcactg
      481 gtgtttcaga taaaagttat tttcacaggc gacaacttca aaaggatagg tttgatgctg
      541 acgtcgatat ctttcacttt agggattgct acagttacca tgtattttgt aagcgctgtt
      601 aaaggtatga ttgtgactta taatgatgtt agtgccaccc aagataaata cttcaatgca
      661 tccacaattt tacttgcatc ctcaataaac tttatgtcat ttgtcctggt agttaaattg
      721 attttagcta ttagatcaag aagattcctt ggtctcaagc agttcgatag tttccatatt
      781 ttactcataa tgtcatgtca atctttgttg gttccatcga taatattcat cctcgcatac
      841 agtttgaaac caaaccaggg aacagatgtc ttgactactg ttgcaacatt acttgctgta
      901 ttgtctttac cattatcatc aatgtgggcc acggctgcta atggagggtc aggcacaggt
      961 ggctctccat ggggttcaga ttacaaggat gacgacgata aggggtctgg aatggtgagc
     1021 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     1081 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     1141 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     1201 accctgagcc acggcgtcca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     1261 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     1321 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     1381 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     1441 tacaacttca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     1501 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     1561 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacagcca ctacctgagc
     1621 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     1681 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagcc atggggatct
     1741 ggcacaggcg ggatgaatgg agctatagga ggtgaccttt tgctcaattt tcctgacatg
     1801 tcggtcctag agcgccaaag ggctcacctc aagtacctca atcccacctt tgattctcct
     1861 ctcgccggct tctttgccga ttcttcaatg attaccggcg gcgagatgga cagctatctt
     1921 tcgactgccg gtttgaatct tccgatgatg tacggtgaga cgacggtgga aggtgattca
     1981 agactctcaa tttcgccgga aacgacgctt gggactggaa atttcaaggc agcgaagttt
     2041 gatacagaga ctaaggattg taatgaggcg gcgaagaaga tgacgatgaa cagagatgac
     2101 ctagtagaag aaggagaaga agagaagtcg aaaataacag agcaaaacaa tgggagcaca
     2161 aaaagcatca agaagatgaa acacaaagcc aagaaagaag agaacaattt ctctaatgat
     2221 tcatctaaag tgacgaagga attggagaaa acggattata ttcatggtgg ctctggtagc
     2281 ttcaacgctc gcaggaagct ggctggtgct atcctgacta ccatgctctt cacaagaaac
     2341 ttcagcggca gctttaatgc gcggcgcaag ctagccggag ccatacttac gacaatgttg
     2401 tttaccagaa atttttcgga gctggccgag aagctggccg gcctggacat caacggtgga
     2461 gctagcggca gccgggcgac caccctggaa cgtattgaaa agagctttgt gattaccgat
     2521 ccgcgtctgc cggataaccc gattattttt gtgagcgata gctttctgca gctgaccgaa
     2581 tatagccgtg aagaaattct gggccgtaac tgccgttttc tgcagggccc ggaaaccgat
     2641 cgtgcgaccg tgcgtaaaat tcgtgatgcg attgataacc agaccgaagt gaccgtgcag
     2701 ctgattaact ataccaaaag cggcaaaaaa ttttggaacc tgtttcatct gcagccgatg
     2761 cgtgatcaga aaggcgatgt gcagtatttt attggcgtgc agctggatgg taccgaacgt
     2821 gtgcgtgatg cggcggaacg tgaagctgtg atgctggtca agaaaaccgc ggaggaaatc
     2881 gacgaagccg ctaaggaaaa tctctacttc cagatgggcg ggcctatgaa agcgttaacg
     2941 gccaggcaac aagaggtgtt tgatctcatc cgtgatcaca tcagccagac aggtatgccg
     3001 ccgacgcgtg cggaaatcgc gcagcgtttg gggttccgtt ccccaaacgc ggctgaagaa
     3061 catctgaagg cgctggcacg caaaggcgtt attgaaattg tttccggcgc atcacgcggg
     3121 attcgtctgt tgcaggaaga ggaagaaggg ttgccgctgg taggtcgtgt ggctgccggt
     3181 gaaccacttc tggcgcaaca gcatattgaa ggtcattatc aggtcgatcc ttccttattc
     3241 aagccgaatg ctgatttcct gctgcgcgtc agcgggatgt cgatgaaaga catcggcatt
     3301 atggatggtg acttgctggc agtgcataaa actcaggatg tacgtaacgg tcaggtcgtt
     3361 gtcgcacgta ttgatgacga ggttaccgtt aagcgcctga aaaaacaggg caataaagtc
     3421 gaactgttgc cagaaaatag cgagtttaaa ccaattgtcg tagatcttcg tcagcagagc
     3481 ttcaccattg aagggctggc ggttggggtt attcgcaacg gcgactggct gggtgatatc
     3541 atgcaggatc tgccgggcaa cgataacagc accgcgggcg cgcgtacgaa aaacaattac
     3601 gggtctacca tcgagggcct gctcgatctc ccggacgacg acgcccccga agaggcgggg
     3661 ctggcggctc cgcgcctgtc ctttctcccc gcgggacaca cgcgcagact gtcgacggcc
     3721 cccccgaccg atgtcagcct gggggacgaa ttgcacttag acggcgagga cgtggcgatg
     3781 gcgcatgccg acgcgctaga cgatttcgat ctggacatgt tgggggacgg ggattccccg
     3841 ggtccgggat ttacccccca cgactccgcc ccctacggcg ctctggatat ggccgacttc
     3901 gagtttgagc agatgtttac cgatgccctt ggaattgacg agtacggtgg gaagctctaa
     3961 tagctcgaga caggcccctt ttcctttgtc tatatcatgt aattagttat gtcacgctta
     4021 cattcacgcc ctcctcccac atccgctcta accgaaaagg aaggagttag acaacctgaa
     4081 gtctaggtcc ctatttattt tttttaatag ttatgttagt attaagaacg ttatttatat
     4141 ttcaaatttt tctttttttt ctgtacaaac gcgtgtacgc atgtaacatt atactgaaaa
     4201 ccttgcttga gaaggttttg ggacgctcga aggctttaat ttgcaaggtt cgcagtttac
     4261 actctcatcg tcgctctcat catcgcttcc gttgttgttt tccttagtag cgtctgcttg
     4321 gtacccagct tcggccgtat ttacagataa cgatgtcgtt attatatata tatatatata
     4381 ttgtcacttg tacccagctt ttgttccctt tagtgagggt taatttcgag cttggcgtaa
     4441 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaacagc tgtaatatat
     4501 agtagtaacc tgaaaaatag tcaatgcccg tggttcattt gccaatagaa cagtcgatgt
     4561 gatcaatgta cacgaaagtc aatataaaat atccaaatag ccaatggtgt caggacggct
     4621 aaggcaaatg caactttgaa atacttattc ggtttgcatc ttggcgcgcc tcttcctgca
     4681 ggcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     4741 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     4801 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     4861 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     4921 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     4981 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     5041 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     5101 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     5161 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     5221 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     5281 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     5341 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     5401 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     5461 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     5521 tttgtttaaa cctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     5581 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     5641 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     5701 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     5761 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     5821 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     5881 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     5941 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     6001 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     6061 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     6121 tccgatggtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     6181 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     6241 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     6301 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     6361 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     6421 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     6481 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     6541 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     6601 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     6661 aaaagtgcca cctgacgtcg aaagaacgcg gcattagcaa catttgcgaa gcgaacgatt
     6721 ttgcgaactg ctattaatta actctggcgc gcctaacttt ttgtgtggtg ccctcctcct
     6781 tgtcaatatt aatgttaaag tgcaattctt tttccttatc acgttgagcc attagtatca
     6841 atttgcttac ctgtattcct ttacatcctc ctttttctcc ttcttgataa atgtatgtag
     6901 attgcgtata tagtttcgtc taccctatga acatattcca ttttgtaatt tcgtgtcgca
     6961 tatgtttcta ttatgaattt catttataaa gtttatgtac aaatatcata aaaaaagaga
     7021 atctttttaa gcaaggattt tcttaacttc ttcggcgaca gcatcaccga cttcggtggt
     7081 actgttggaa ccacctaaat caccagttct gatacctgca tccaaaacct ttttaactgc
     7141 atcttcaatg gccttacctt cttcaggcaa gttcaatgac aatttcaaca tcattgcagc
     7201 agacaagata gtggcgatag ggttgacctt attctttggc aaatctggag cagaaccgtg
     7261 gcatggttcg tacaaaccaa atgcggtgtt cttgtctggc aaagaggcca aggacgcaga
     7321 tggcaacaaa cccaaggaac ctgggataac ggaggcttca tcggagatga tgtcaccaaa
     7381 catgttgctg gtgattataa taccatttag gtgggttggg ttcttaacta ggatcatggc
     7441 ggcagaatca atcaattgat gttgaacctt caatgtagga aattcgttct tgatggtttc
     7501 ctccacagtt tttctccata atcttgaaga ggccaaaaca ttagctttat ccaaggacca
     7561 aataggcaat ggtggctcat gttgtagggc catgaaagcg gccattcttg tgattctttg
     7621 cacttctgga acggtgtatt gttcactatc ccaagcgaca ccatcaccat cgtcttcctt
     7681 tctcttacca aagtaaatac ctcccactaa ttctctgaca acaacgaagt cagtaccttt
     7741 agcaaattgt ggcttgattg gagataagtc taaaagagag tcggatgcaa agttacatgg
     7801 tcttaagttg gcgtacaatt gaagttcttt acggattttt agtaaacctt gttcaggtct
     7861 aacactacca gtaccccatt taggaccacc cacagcacct aacaaaacgg catcagcctt
     7921 cttggaggct tccagcgcct catctggaag tggaacacct gtagcatcga tagcagcacc
     7981 accaattaaa tgattttgga aatcgaactt gacattggaa cgaacatcag aaatagcttt
     8041 aagaacctta atggcttcgg ctgtgatttc ttgaccaacg tggtcacctg gcaaaacgac
     8101 gatcttctta ggggcagaca ttagaatggt atatccttga aatatatata tatattgctg
     8161 aaatgtaaaa ggtaagaaaa gttagaaagt aagacgattg ctaaccacct attggaaaaa
     8221 acaataggtc cttaaataat attgtcaact tcaagtattg tgatgcaagc atttagtcat
     8281 gaacgcttct ctattctata tgaaaagccg gttccggcgc tctcaccttt cctttttctc
     8341 ccaatttttc agttgaaaaa ggtatatgcg tcaggcgacc tctgaaatta acaaaaaatt
     8401 tccagtcatc gaatttgatt ctgtgcgata gcgcccctgt gtgttctcgt tatgttgagg
     8461 aaaaaaataa tggttgctaa gagattcgaa ctcttgcatc ttacgatacc tgagtattcc
     8521 cacagttaac tgcggtcaag atatttcttg aatcaggcgc cttagaccgc tcggccaaac
     8581 aaccaattac ttgttgagaa atagagtata attatcctat aaatataacg tttttgaaca
     8641 cacatgaaca aggaagtaca ggacaattga ttttcgatcg gatgtgctgc aaggcgatta
     8701 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg
     8761 taatacgact cactataggg cgaattggag aaacagaata atatataaac ctgtataata
     8821 taaccttgaa gactatattt cttttcgcgg ccgcctatag ggcgaattgg agctcccgct
     8881 ctagacagtt cgagtttatc attatcaata ctgccatttc aaagaatacg taaataatta
     8941 atagtagtga ttttcctaac tttatttagt caaaaaatta gccttttaat tctgctgtaa
     9001 cccgtacatg cccaaaatag ggggcgggtt acacagaata tataacatcg taggtgtctg
     9061 ggtgaacagt ttattcctgg catccactaa atataatgga gcccgctttt taagctggca
     9121 tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac catcagttca
     9181 taggtccatt ctcttagcgc aactacagag aacaggggca caaacaggca aaaaacgggc
     9241 acaacctcaa tggagtgatg caacctgcct ggagtaaatg atgacacaag gcaattgacc
     9301 cacgcatgta tctatctcat tttcttacac cttctattac cttctgctct ctctgatttg
     9361 gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct acttgactaa
     9421 taagtatata aagacggtag gtattgattg taattctgta aatctatttc ttaaacttct
     9481 taaattctac ttttatagtt agtctttttt ttagttttaa aacaccaaga acttagtttc
     9541 gaataaacac acataaacaa acaaa
//
