LOCUS       sequence-26752-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Nam HS, Benezra R
  TITLE     High levels of Id1 expression define B1 type adult neural stem 
            cells.
  JOURNAL   Cell Stem Cell. 2009 Nov 6. 5(5):515-26.
  PUBMED    19896442
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2009 Nov 6. 5(5):515-26."
COMMENT     Sequence Label: H2B-Strawberry
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             100..477
                     /codon_start=1
                     /gene="HIST1H2BB"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPSKSAPAPKKGSKKAITKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSSK"
     regulatory      490..499
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(641..659)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 attagcagag ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata
       61 cgactcacta tagggagacc caagcttggt accgccacca tgcctgaacc ctctaagtct
      121 gctccagccc ctaaaaaggg ttctaagaag gctatcacta aggcgcagaa gaaggatggt
      181 aagaagcgta agcgcagccg caaggagagc tattctatct atgtgtacaa ggttctgaag
      241 caggtccacc ccgacaccgg catctcatcc aaggccatgg ggatcatgaa ctccttcgtc
      301 aacgacatct tcgagcgcat cgcgggcgag gcttctcgcc tggctcacta caataagcgc
      361 tcgaccatca cctccaggga gattcagacg gctgtgcgcc tgctgctgcc tggggagctg
      421 gctaagcatg ctgtgtccga gggcactaag gcagttacca agtacactag ctctaaggat
      481 ccaccggtcg ccaccatggt gagcaagggc gaggagaata acatggccat catcaaggag
      541 ttcatgcgct tcaaggtgcg catggagggc tccgtgaacg gccacgagtt cgagatcgag
      601 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
      661 ggtggccccc tgcccttcgc ctgggacatc ctaaccccca acttcaccta cggctccang
      721 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
      781 ncnngtggga gcgcgtgatg aactncnaga ngncggcgtg gtgacgtgac cagganc
//
