LOCUS       sequence-267520-        9358 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SPARK TF component.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9358)
  AUTHORS   Sanchez MI, Ting AY
  TITLE     Directed evolution improves the catalytic efficiency of TEV 
            protease.
  JOURNAL   Nat Methods. 2019 Dec 9. pii: 10.1038/s41592-019-0665-7. doi: 
            10.1038/s41592-019-0665-7.
  PUBMED    31819267
REFERENCE   2  (bases 1 to 9358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2019 Dec 9. pii: 10.1038/s41592-019-0665-7. doi: 
            10.1038/s41592-019-0665-7."
COMMENT     Sequence Label: CMV:beta-2Ar-hLOV-TEVcs(ENLYFQ/M)-GAL4-V5
FEATURES             Location/Qualifiers
     source          1..9358
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2238..2258
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQM"
     CDS             2271..2294
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2295..2732
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKR
                     SPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKD
                     AVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             2733..3074
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             3075..3116
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3135..3723
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3188..3208)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3606..3617)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3795..4028
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4106..4227
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4143..4162)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4197..4216
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4267..4402
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4422..4441)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4423..4441)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4451..4468)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4451..4467)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4460..4482)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4609..5064
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4696..4715)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4906..4927
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5090..5194
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5195..5263,5264..6055)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5413..5432)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6226..6814
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6715..6734
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6968..6985
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7102..7123
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7138..7143,7144..7161,7162..7168)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7176..7192
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7181..7203
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7200..7216
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7200..7216
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7235..7255
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7237..7255
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7283..7509
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7510..7690
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7737..7862
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8355..8588
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8773..8817
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9125..9242
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact gtcgggatcc accatggggc
      541 aacccgggaa cggcagcgcc ttcttgctgg cacccaatag aagccatgcg ccggaccacg
      601 acgtcacgca gcagagggac gaggtgtggg tggtgggcat gggcatcgtc atgtctctca
      661 tcgtcctggc catcgtgttt ggcaatgtgc tggtcatcac agccattgcc aagttcgagc
      721 gtctgcagac ggtcaccaac tacttcatca cttcactggc ctgtgctgat ctggtcatgg
      781 gcctggcagt ggtgcccttt ggggccgccc atattcttat gaaaatgtgg acttttggca
      841 acttctggtg cgagttttgg acttccattg atgtgctgtg cgtcacggcc agcattgaga
      901 ccctgtgcgt gatcgcagtg gatcgctact ttgccattac ttcacctttc aagtaccaga
      961 gcctgctgac caagaataag gcccgggtga tcattctgat ggtgtggatt gtgtcaggcc
     1021 ttacctcctt cttgcccatt cagatgcact ggtaccgggc cacccaccag gaagccatca
     1081 actgctatgc caatgagacc tgctgtgact tcttcacgaa ccaagcctat gccattgcct
     1141 cttccatcgt gtccttctac gttcccctgg tgatcatggt cttcgtctac tccagggtct
     1201 ttcaggaggc caaaaggcag ctccagaaga ttgacaaatc tgagggccgc ttccatgtcc
     1261 agaaccttag ccaggtggag caggatgggc ggacggggca tggactccgc agatcttcca
     1321 agttctgctt gaaggagcac aaagccctca agacgttagg catcatcatg ggcactttca
     1381 ccctctgctg gctgcccttc ttcatcgtta acattgtgca tgtgatccag gataacctca
     1441 tccgtaagga agtttacatc ctcctaaatt ggataggcta tgtcaattct ggtttcaatc
     1501 cccttatcta ctgccggagc ccagatttca ggattgcctt ccaggagctt ctgtgcctgc
     1561 gcaggtcttc tttgaaggcc tatgggaatg gctactccag caacggcaac acaggggagc
     1621 agagtggata tcacgtggaa caggagaaag aaaataaact gctgtgtgaa gacctcccag
     1681 gcacggaaga ctttgtgggc catcaaggta ctgtgcctag cgataacatt gattcacaag
     1741 ggaggaattg tagtacaaat gactcactgc tggagctggc cgagaagctg gccggcctgg
     1801 acatcaacgg tggagctagc gagtttcggg caaccacact ggaacggatc gagaaaagct
     1861 tcgtgattac tgatccgaga ctgcctgaca acccaatcat ttttgtgagc gattccttcc
     1921 tgcagctgac agaatattct cgggaagaga tcctggggcg caattgccgt tttctgcagg
     1981 gacccgagac agaccgtgcc actgttcgga aaatcagaga tgctattgac aaccagactg
     2041 aagtgaccgt tcagctgatc aattatacca agagcggcaa gaagttctgg aacgtgttcc
     2101 acctgcagcc gatgcgcgat tataagggcg acgtccagta cttcattggc gtgcagctgg
     2161 atggcaccga acgtcttcat ggcgccgctg agcgtgaggc ggtctgcctg gtcaaaaaga
     2221 cagcctttca gattgctgag aacctgtact tccagatggg cggaggatcc gactacaagg
     2281 atgacgacga taagaagcta ctgtcttcta tcgaacaagc atgcgatatt tgccgactta
     2341 aaaagctcaa gtgctccaaa gaaaaaccga agtgcgccaa gtgtctgaag aacaactggg
     2401 agtgtcgcta ctctcccaaa accaaaaggt ctccgctgac tagggcacat ctgacagaag
     2461 tggaatcaag gctagaaaga ctggaacagc tatttctact gatttttcct cgagaagacc
     2521 ttgacatgat tttgaaaatg gattctttac aggatataaa agcattgtta acaggattat
     2581 ttgtacaaga taatgtgaat aaagatgccg tcacagatag attggcttca gtggagactg
     2641 atatgcctct aacattgaga cagcatagaa taagtgcgac atcatcatcg gaagagagta
     2701 gtaacaaagg tcaaagacag ttgactgtat cggccaattt taatcaaagt gggaatattg
     2761 ctgatagctc attgtccttc actttcacta acagtagcaa cggtccgaac ctcataacaa
     2821 ctcaaacaaa ttctcaagcg ctttcacaac caattgcctc ctctaacgtt catgataact
     2881 tcatgaataa tgaaatcacg gctagtaaaa ttgatgatgg taataattca aaaccactgt
     2941 cacctggttg gacggaccaa actgcgtata acgcgtttgg aatcactaca gggatgttta
     3001 ataccactac aatggatgat gtatataact atctattcga tgatgaagat accccaccaa
     3061 acccaaaaaa agagggcaag ccgatcccta accctctgct gggcctggac agcacttaga
     3121 cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     3181 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     3241 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     3301 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     3361 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     3421 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     3481 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     3541 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     3601 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     3661 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     3721 cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     3781 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     3841 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     3901 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     3961 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     4021 ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     4081 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     4141 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     4201 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     4261 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     4321 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     4381 tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt
     4441 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     4501 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     4561 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     4621 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     4681 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     4741 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     4801 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     4861 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     4921 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     4981 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     5041 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     5101 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     5161 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5221 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     5281 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     5341 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     5401 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     5461 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5521 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5581 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5641 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5701 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5761 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5821 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5881 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5941 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     6001 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     6061 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6121 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6181 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6241 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6301 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6361 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6421 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6481 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6541 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6601 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6661 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6721 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6781 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6841 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     6901 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     6961 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     7021 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     7081 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     7141 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     7201 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
     7261 aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg
     7321 gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg
     7381 gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg
     7441 attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga
     7501 tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7561 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7621 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7681 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     7741 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     7801 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     7861 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     7921 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     7981 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     8041 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     8101 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     8161 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
     8221 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     8281 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     8341 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     8401 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     8461 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     8521 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     8581 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     8641 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     8701 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     8761 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     8821 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     8881 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     8941 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgagagcgct gccgtctgga
     9001 ccgatggctg tgtggaagtg ctcgccgata gtggaaacag acgccccagc actcgtccta
     9061 gggcaaagga tctgcagtaa tgagaattct agatcttgag acaaatggca gtattcatcc
     9121 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     9181 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     9241 ttcgggttta ttacagggac agcagagatc cactttggcg ccggctcgag ggggcccggg
     9301 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccg
//
