LOCUS       sequence-267523-        9851 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration of PEX1-2GFP as an additional copy in genome, use 
            auxotrophic marker URA3(Candida albicans). Marker for yeast 
            peroxisome..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9851)
  AUTHORS   Zhu J, Zhang ZT, Tang SW, Zhao BS, Li H, Song JZ, Li D, Xie Z
  TITLE     A Validated Set of Fluorescent-Protein-Based Markers for Major 
            Organelles in Yeast (Saccharomyces cerevisiae).
  JOURNAL   MBio. 2019 Sep 3;10(5). pii: mBio.01691-19. doi: 
            10.1128/mBio.01691-19.
  PUBMED    31481383
REFERENCE   2  (bases 1 to 9851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2019-09-3"; volume: "10(5). pii: mBio.01691-19. doi"; pages: 
            " 10.1128/mBio.01691-19"
COMMENT     Sequence Label: ZJOM20
FEATURES             Location/Qualifiers
     source          1..9851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3955..4668
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
     CDS             4705..5421
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             4705..5418
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      5442..5629
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5497..5521)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    5912..5945
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      6900..7097
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    7151..7184
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(7238..7257)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7239..7257)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7344..7361)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7515..8103)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7595..7614)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8274..9065,9066..9134))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8897..8916
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9135..9239)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9307..9325
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9363..9385)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9485..9504
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        9585..9603
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     9585..9602
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 acttggccat ggagtctaat tgctatattt ttcttttgaa aacaagatat ataaataatg
       61 cttgagctgg agcatttacg ttgtaaatgt ataattagtg taagaaaccg ctgggcccta
      121 tttgttgatg aagtatttac ttaaatgtaa cagaatatac tggaagtccc ccctcgggca
      181 catggaaatt cactaaaagg aattggtaat gcatatgata ttactaccgt ttctcatttc
      241 actttatatg ctttcagtga ttgtgttact acgtgacagt tcttacaatt cagcgccgtt
      301 actctctatg agtgctctca ttatttatat catattctta cacttcatac aattatatat
      361 actcgtacca tggatattag aggatagttg gtttttgttt aaactaattc tatgtaacct
      421 cgatggcatg agtccataat actgtactgg cgtatgccct tgtttgtggt ctttgtgcaa
      481 ccatttcagg ctttcttatt tgcacagggc agttgcgacc ctgataaaaa aaaatggctg
      541 atcaatcggc gtggacaaaa aaaaacatcg atttctatat tcatggtaat ctgtcgaatt
      601 ctccaaccaa gttagctgga ctttattgtc ttatatccga ttactatcgc gatttattct
      661 tgtgcgtagc cgcaattata aaggtccgga atatctctcc gactattgtc atcgtcgaat
      721 ttcttctgga agggacggca gtaacaagaa acacctgagg aactgctctt tcaaatgacg
      781 acgaccaaga ggttgaagtt tgagaatctg agaatccaat tctccaatgc catagtagga
      841 aactttttaa ggttaccgca ttcaattata aacgtgttag agtcaaccaa ctacgctatc
      901 caggaattcg gtatagcagt ccattctcat aattcagata tacctattgt tcatcttgga
      961 tgggatggcc atgattctgg atcgagtgag aatgttgttc ttatcaatcc tgttttggcc
     1021 acagtatatg atttaaatca aaagtctcca ttggtggact tatacatcca gcgatatgac
     1081 cacacacacc tggctacgga ggtgtatgtc acgccagaaa cgagtgatga ttgggaaatt
     1141 atcgatgcca atgcgatgag gttccaaaat ggtgagattt tgcaccaaac ccgtatagtt
     1201 accccaggtg agactttgat ttgttatttg gaaggaattg ttacaaagtt caaaattgac
     1261 agggtggagc catcaatgaa gtctgcgagg atcacagatg gttccttggt tgttgtggca
     1321 ccaaaagtca ataaaacacg tttggtgaag gctgagtatg gtcatagtaa taagacaata
     1381 ctaaaaaatg gggctatcca acttttaaag aaggtaatat tgaggagtac tgtgtgcaaa
     1441 atggacttcc ctaaagataa cctttttgtc gtatatatca gtgacggggc acaattgcct
     1501 tctcagaagg gctacgcttc tatcgtaaaa tgtagcttaa ggcagtccaa gaaaagcgat
     1561 tcagataata aatcagttgg tatcccttcg aagaagattg gggtttttat aaaatgtgat
     1621 agtcagatac ccgaaaatca cattgcatta agctctcatc tttgggatgc attctttacg
     1681 cacccaatga acggagcaaa aatcaaacta gagtttctac aaatgaatca agcaaatata
     1741 atatccggaa gaaatgcaac agtcaatatc aaatattttg gcaaggacgt tcctaccaag
     1801 agtggggatc agtattctaa gttgttgggc gggagcttat tgaccaataa cttaatactt
     1861 ccaacagagc aaattataat cgaaataaaa aaaggtgaat ccgagcagca gttgtgtaat
     1921 ttgaatgaaa tttccaatga atccgttcaa tggaaagtaa cccaaatggg taaagaagaa
     1981 gttaaagata ttatcgaaag gcatttgcct aaacattacc acgtcaaaga aactggcgaa
     2041 gtttcgcgca ctagtaaaga tgaagatgat ttcattacgg taaatagtat caagaaagaa
     2101 atggtcaatt atttgacatc gcctattatt gccacaccag ctattatatt agatggtaag
     2161 caagggatcg gtaaaacaag gttattaaaa gagcttataa atgaagtgga aaaagatcat
     2221 cacatcttcg ttaaatatgc ggattgtgaa acattgcacg agacatcaaa tttagataaa
     2281 acccaaaaat taattatgga atggtgttct ttttgttatt ggtatggtcc ttctttgatt
     2341 gtgttggata acgttgaggc tctatttgga aaacctcaag ccaatgacgg agatccttct
     2401 aataatggcc aatgggataa tgcaagcaaa cttttgaatt ttttcatcaa tcaggtgaca
     2461 aagatattta acaaggataa taaacgaatc agagttttat tttcaggcaa gcagaaaact
     2521 caaattaatc cgctattgtt tgataagcat tttgtttcag agacgtggtc tttgagagca
     2581 cctgacaaac atgcaagagc gaagttactg gagtattttt tctcgaaaaa ccagatcatg
     2641 aaactaaatc gagatttgca attcagtgat ttgtcgttgg agacagaagg attttcgcca
     2701 ctagatttgg aaatattcac agaaaagatt ttctacgact tgcagttgga aagagattgt
     2761 gataatgttg taacaaggga acttttttcg aagtcgctta gtgcgtttac accatctgcg
     2821 ttgcgtggag taaagctaac aaaagaaacg aacatcaaat ggggggatat tggtgcttta
     2881 gcaaatgcca aagatgtcct tctagaaacc ttagagtggc ccacaaaata tgagcctatt
     2941 ttcgttaatt gtcccctaag attgagatca ggaatcttgc tttacggtta tcctggttgt
     3001 ggtaaaacgc ttctggcgag cgccgtggca caacaatgtg ggttaaactt tatctccgtt
     3061 aagggaccag agattttaaa caagtttata ggtgccagcg aacaaaacat aagagaattg
     3121 tttgaaaggg cacagtccgt caaaccctgt attctatttt ttgacgagtt cgattctatt
     3181 gcgccaaaga gagggcatga ctccactggt gtcacagacc gtgtagtcaa tcaattattg
     3241 acccaaatgg atggtgccga gggccttgat ggtgtatata tactagcagc tacaagtaga
     3301 cctgatttga ttgatagcgc attgttaaga ccgggaagat tagacaaaag tgtgatctgt
     3361 aatataccga ctgaatcaga gaggttagat atcttgcaag ctatcgtcaa ttcaaaagac
     3421 aaggatacgg gacaaaaaaa atttgcattg gagaaaaatg cagacttgaa gttgatcgct
     3481 gaaaagacag ctggtttttc gggtgctgat ttacaggggc tctgctacaa tgcttatttg
     3541 aaatctgtac ataggtggct gtctgctgca gaccaatcgg aagtggtacc cggcaatgat
     3601 aatatagaat acttcagtat caatgagcac gggcgtagag aagaaaatag actacggcta
     3661 aagactctat tgcagcaaga tgtggtgcac gagacaaaga caagcacttc agctgcttct
     3721 gagctgacag ctgtggtcac aataaacgat ttattggaag cctgtcaaga gacaaaacct
     3781 agtatttcaa caagtgagtt ggtcaaatta agggggattt acgataggtt ccagaaggat
     3841 aggaacggtg agatgcccaa tggtgagaat tccatcgaca ttggtagccg actctccctt
     3901 atggcttcgt acgctgcagg tcgacggatc ggtgacggtg ctggtttaat taacatgtct
     3961 aaaggtgaag aattattcac tggtgttgtc ccaattttgg ttgaattaga tggtgatgtt
     4021 aatggtcaca aattttctgt ctccggtgaa ggtgaaggtg atgctactta cggtaaattg
     4081 accttaaaat ttatttgtac tactggtaaa ttgccagttc catggccaac cttagtcact
     4141 actttaactt atggtgttca atgtttttct agatacccag atcatatgaa acaacatgac
     4201 tttttcaagt ctgccatgcc agaaggttat gttcaagaaa gaactatttt tttcaaagat
     4261 gacggtaact acaagaccag agctgaagtc aagtttgaag gtgatacctt agttaataga
     4321 atcgaattaa aaggtattga ttttaaagaa gatggtaaca ttttaggtca caaattggaa
     4381 tacaactata actctcacaa tgtttacatc atggctgaca aacaaaagaa tggtatcaaa
     4441 gttaacttca aaattagaca caacattgaa gatggttctg ttcaattagc tgaccattat
     4501 caacaaaata ctccaattgg tgatggtcca gtcttgttac cagacaacca ttacttatcc
     4561 actcaatctg ccttatccaa agatccaaac gaaaagagag accacatggt cttgttagaa
     4621 tttgttactg ctgctggtat tacccatggt attgatgaat tgtacaaaat tccagttcca
     4681 acgcgtgatc tagagaagcc attcatgtct aaaggtgaag aattattcac tggtgttgtc
     4741 ccaattttgg ttgaattaga tggtgatgtt aatggtcaca aattttctgt ctccggtgaa
     4801 ggtgaaggtg atgctactta cggtaaattg accttaaaat ttatttgtac tactggtaaa
     4861 ttgccagttc catggccaac cttagtcact actttaactt atggtgttca atgtttttct
     4921 agatacccag atcatatgaa acaacatgac tttttcaagt ctgccatgcc agaaggttat
     4981 gttcaagaaa gaactatttt tttcaaagat gacggtaact acaagaccag agctgaagtc
     5041 aagtttgaag gtgatacctt agttaataga atcgaattaa aaggtattga ttttaaagaa
     5101 gatggtaaca ttttaggtca caaattggaa tacaactata actctcacaa tgtttacatc
     5161 atggctgaca aacaaaagaa tggtatcaaa gttaacttca aaattagaca caacattgaa
     5221 gatggttctg ttcaattagc tgaccattat caacaaaata ctccaattgg tgatggtcca
     5281 gtcttgttac cagacaacca ttacttatcc actcaatctg ccttatccaa agatccaaac
     5341 gaaaagagag accacatggt cttgttagaa tttgttactg ctgctggtat tacccatggt
     5401 atggatgaat tatataaata aggcgcgcca cttctaaata agcgaatttc ttatgattta
     5461 tgatttttat tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact
     5521 cttaggtttt aaaacgaaaa ttcttattct tgagtaactc tttcctgtag gtcaggttgc
     5581 tttctcaggt atagtatgag gtcgctctta ttgaccacac ctctaccggc agatccgcta
     5641 gggataacag ggtaatatag atctgtttag cttgcctcgt ccccgccggg tcacccggcc
     5701 agcgacatgg aggcccagaa taccctcctt gacagtcttg acgtgcgcag ctcaggggca
     5761 tgatgtgact gtcgcccgta catttagccc atacatcccc atgtataatc atttgcatcc
     5821 atacattttg atggccgcac ggcgcgaagc aaaaattacg gctcctcgct gcggacctgc
     5881 gagcagggaa acgctcccct cacagacgcg tataacttcg tataatgtat gctatacgaa
     5941 gttattgaat tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc
     6001 actgaggttc ttctttcata tacttccttt taaaatcttg ctaggataca gttctcacat
     6061 cacatccgaa cataaacaac catgacagtc aacactaaga cctatagtga gagagcagaa
     6121 actcatgcct caccagtagc acaacgatta tttcgattaa tggaactgaa gaaaaccaat
     6181 ttatgtgcat caattgatgt tgataccact aaggaattcc ttgaattaat tgataaattg
     6241 ggtccttatg tatgcttaat caagactcat attgatataa tcaatgattt ttcctatgaa
     6301 tccactattg aaccattatt agaactttca cgtaaacatc aatttatgat ttttgaagat
     6361 agaaaatttg ctgatattgg taataccgtg aagaaacaat atattggtgg agtttataaa
     6421 attagtagtt gggcagatat tactaatgct catggtgtca ctgggaatgg agtagttgaa
     6481 ggattaaaac agggagctaa agaaaccacc accaaccaag agccaagagg gttattgatg
     6541 ttagctgaat tatcatcagt gggatcatta gcatatggag aatattctca aaaaactgtt
     6601 gaaattgcta aatccgataa ggaatttgtt attggattta ttgcccaacg tgatatgggt
     6661 ggacaagaag aaggatttga ttggcttatt atgacacctg gagttggatt agatgataaa
     6721 ggtgatggat taggacaaca atatagaact gttgatgaag ttgttagcac tggaactgat
     6781 attatcattg ttggtagagg attgtttggt aaaggaagag atccagatat tgaaggtaaa
     6841 aggtatagag atgctggttg gaatgcttat ttgaaaaaga ctggccaatt ataatcagta
     6901 ctgacaataa aaagattctt gttttcaaga acttgtcatt tgtatagttt ttttatattg
     6961 tagttgttct attttaatca aatgttagcg tgatttatat tttttttcgc ctcgacatca
     7021 tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa
     7081 tgctggtcgc tatactgctg tcgattcgat actaacgccg ccatccagtg tcgaaaacga
     7141 gctcgaattc ataacttcgt ataatgtatg ctatacgaag ttatatcgat gatatcagat
     7201 ccactagtgg cctatgcggc cgcggatctg ccggtctccc tatagtgagt cgtattaatt
     7261 tcgataagcc aggttaacct gcattaatga atcggccaac gcgcggggag aggcggtttg
     7321 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7381 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7441 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7501 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7561 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7621 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7681 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7741 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7801 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7861 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7921 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     7981 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     8041 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     8101 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     8161 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     8221 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     8281 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     8341 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8401 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8461 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8521 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8581 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     8641 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8701 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     8761 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     8821 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     8881 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     8941 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     9001 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     9061 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     9121 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     9181 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     9241 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     9301 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     9361 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     9421 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     9481 tatgcggcat cagagcagat tgtactgaga gtgcaccata tggacatatt gtcgttagaa
     9541 cgcggctaca attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat
     9601 agaacgcggc cgccagctga agcttatgac agcagcaagg gaaacaaaaa tcttttaaat
     9661 caaattaatt gtttaaataa tatactatgt aagcatcgat tctcctaact attataacca
     9721 aaggttgatt tgaagccatt ggcattgtat tagaattgat ttgtttcttc agtgtaaaaa
     9781 agttaaaaat aagcgacccc agtgagggtt gaactcacga tcttgcgatt aacagtcgca
     9841 cgccttaacc a
//
