LOCUS       sequence-267531-       16594 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector with CMV promoter driving beta globin 
            intron, HA tag, BRCA2 ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16594)
  AUTHORS   Chen J, Silver DP, Walpita D, Cantor SB, Gazdar AF, Tomlinson G, 
            Couch FJ, Weber BL, Ashley T, Livingston DM, Scully R
  TITLE     Stable interaction between the products of the BRCA1 and BRCA2 tumor
            suppressor genes in mitotic and meiotic cells.
  JOURNAL   Mol Cell. 1998 Sep;2(3):317-28.
  PUBMED    9774970
REFERENCE   2  (bases 1 to 16594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "1998-09"; volume: "2(3)"; pages: "317-28"
COMMENT     Sequence Label: pcDNA3-beta-HA-BRCA2
FEATURES             Location/Qualifiers
     source          1..16594
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          918..1490
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             1542..1568
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             1542..1568
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1548..1574
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     promoter        complement(12147..12165)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(12148..12165)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(12185..12202)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12191..12415
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      12461..12889
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12548..12567)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12758..12779
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12898..12918)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12903..13232
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      13083..13218
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     13145..13164
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             13299..14093
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(13353..13372)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     13963..13982
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    14267..14388
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14304..14323)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14358..14377
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(14437..14453)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14437..14453)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14450..14472)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14461..14477
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14485..14491,14492..14509,14510..14515))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14530..14551
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14668..14685)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14839..15427)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14919..14938)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15598..16389,16390..16458))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16221..16240
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16459..16563)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctagagtcg
      901 atcctgagaa cttcagggtg agtttgggga cccttgattg ttctttcttt ttcgctattg
      961 taaaattcat gttatatgga gggggcaaag ttttcagggt gttgtttaga atgggaagat
     1021 gtcccttgta tcaccatgga ccctcatgat aattttgttt ctttcacttt ctactctgtt
     1081 gacaaccatt gtctcctctt attttctttt cattttctgt aactttttcg ttaaacttta
     1141 gcttgcattt gtaacgaatt tttaaattca cttttgttta tttgtcagat tgtaagtact
     1201 ttctctaatc actttttttt caaggcaatc agggtatatt atattgtact tcagcacagt
     1261 tttagagaac aattgttata attaaatgat aaggtagaat atttctgcat ataaattctg
     1321 gctggcgtgg aaatattctt attggtagaa acaactacat cctggtcatc atcctgcctt
     1381 tctctttatg gttacaatga tatacactgt ttgagatgag gataaaatac tctgagtcca
     1441 aaccgggccc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1501 acgtgctggt ttgtgctgtc gaccccaagc ttaccatggc ctacccctac gacgtgcccg
     1561 actacgcctc cctcggatcg tggatcctga tatcctcgac cgccaccatg cctattgggt
     1621 ccaaagagag gccaacattt tttgaaattt ttaagacacg ctgcaacaaa gcagatttag
     1681 gaccaataag tcttaattgg tttgaagaac tttcttcaga agctccaccc tataattctg
     1741 aacctgcaga agaatctgaa cataaaaaca acaattacga accaaaccta tttaaaactc
     1801 cacaaaggaa accatcttat aatcagctgg cttcaactcc aataatattc aaagagcaag
     1861 ggctgactct gccgctgtac caatctcctg taaaagaatt agataaattc aaattagact
     1921 taggaaggaa tgttcccaat agtagacata aaagtcttcg cacagtgaaa actaaaatgg
     1981 atcaagcaga tgatgtttcc tgtccacttc taaattcttg tcttagtgaa agtcctgttg
     2041 ttctacaatg tacacatgta acaccacaaa gagataagtc agtggtatgt gggagtttgt
     2101 ttcatacacc aaagtttgtg aagggtcgtc agacaccaaa acatatttct gaaagtctag
     2161 gagctgaggt ggatcctgat atgtcttggt caagttcttt agctacacca cccaccctta
     2221 gttctactgt gctcatagtc agaaatgaag aagcatctga aactgtattt cctcatgata
     2281 ctactgctaa tgtgaaaagc tatttttcca atcatgatga aagtctgaag aaaaatgata
     2341 gatttatcgc ttctgtgaca gacagtgaaa acacaaatca aagagaagct gcaagtcatg
     2401 gatttggaaa aacatcaggg aattcattta aagtaaatag ctgcaaagac cacattggaa
     2461 agtcaatgcc aaatgtccta gaagatgaag tatatgaaac agttgtagat acctctgaag
     2521 aagatagttt ttcattatgt ttttctaaat gtagaacaaa aaatctacaa aaagtaagaa
     2581 ctagcaagac taggaaaaaa attttccatg aagcaaacgc tgatgaatgt gaaaaatcta
     2641 aaaaccaagt gaaagaaaaa tactcatttg tatctgaagt ggaaccaaat gatactgatc
     2701 cattagattc aaatgtagca catcagaagc cctttgagag tggaagtgac aaaatctcca
     2761 aggaagttgt accgtctttg gcctgtgaat ggtctcaact aaccctttca ggtctaaatg
     2821 gagcccagat ggagaaaata cccctattgc atatttcttc atgtgaccaa aatatttcag
     2881 aaaaagacct attagacaca gagaacaaaa gaaagaaaga ttttcttact tcagagaatt
     2941 ctttgccacg tatttctagc ctaccaaaat cagagaagcc attaaatgag gaaacagtgg
     3001 taaataagag agatgaagag cagcatcttg aatctcatac agactgcatt cttgcagtaa
     3061 agcaggcaat atctggaact tctccagtgg cttcttcatt tcagggtatc aaaaagtcta
     3121 tattcagaat aagagaatca cctaaagaga ctttcaatgc aagtttttca ggtcatatga
     3181 ctgatccaaa ctttaaaaaa gaaactgaag cctctgaaag tggactggaa atacatactg
     3241 tttgctcaca gaaggaggac tccttatgtc caaatttaat tgataatgga agctggccag
     3301 ccaccaccac acagaattct gtagctttga agaatgcagg tttaatatcc actttgaaaa
     3361 agaaaacaaa taagtttatt tatgctatac atgatgaaac attttataaa ggaaaaaaaa
     3421 taccgaaaga ccaaaaatca gaactaatta actgttcagc ccagtttgaa gcaaatgctt
     3481 ttgaagcacc acttacattt gcaaatgctg attcaggttt attgcattct tctgtgaaaa
     3541 gaagctgttc acagaatgat tctgaagaac caactttgtc cttaactagc tcttttggga
     3601 caattctgag gaaatgttct agaaatgaaa catgttctaa taatacagta atctctcagg
     3661 atcttgatta taaagaagca aaatgtaata aggaaaaact acagttattt attaccccag
     3721 aagctgattc tctgtcatgc ctgcaggaag gacagtgtga aaatgatcca aaaagcaaaa
     3781 aagtttcaga tataaaagaa gaggtcttgg ctgcagcatg tcacccagta caacattcaa
     3841 aagtggaata cagtgatact gactttcaat cccagaaaag tcttttatat gatcatgaaa
     3901 atgccagcac tcttatttta actcctactt ccaaggatgt tctgtcaaac ctagtcatga
     3961 tttctagagg caaagaatca tacaaaatgt cagacaagct caaaggtaac aattatgaat
     4021 ctgatgttga attaaccaaa aatattccca tggaaaagaa tcaagatgta tgtgctttaa
     4081 atgaaaatta taaaaacgtt gagctgttgc cacctgaaaa atacatgaga gtagcatcac
     4141 cttcaagaaa ggtacaattc aaccaaaaca caaatctaag agtaatccaa aaaaatcaag
     4201 aagaaactac ttcaatttca aaaataactg tcaatccaga ctctgaagaa cttttctcag
     4261 acaatgagaa taattttgtc ttccaagtag ctaatgaaag gaataatctt gctttaggaa
     4321 atactaagga acttcatgaa acagacttga cttgtgtaaa cgaacccatt ttcaagaact
     4381 ctaccatggt tttatatgga gacacaggtg ataaacaagc aacccaagtg tcaattaaaa
     4441 aagatttggt ttatgttctt gcagaggaga acaaaaatag tgtaaagcag catataaaaa
     4501 tgactctagg tcaagattta aaatcggaca tctccttgaa tatagataaa ataccagaaa
     4561 aaaataatga ttacatgaac aaatgggcag gactcttagg tccaatttca aatcacagtt
     4621 ttggaggtag cttcagaaca gcttcaaata aggaaatcaa gctctctgaa cataacatta
     4681 agaagagcaa aatgttcttc aaagatattg aagaacaata tcctactagt ttagcttgtg
     4741 ttgaaattgt aaataccttg gcattagata atcaaaagaa actgagcaag cctcagtcaa
     4801 ttaatactgt atctgcacat ttacagagta gtgtagttgt ttctgattgt aaaaatagtc
     4861 atataacccc tcagatgtta ttttccaagc aggattttaa ttcaaaccat aatttaacac
     4921 ctagccaaaa ggcagaaatt acagaacttt ctactatatt agaagaatca ggaagtcagt
     4981 ttgaatttac tcagtttaga aagccaagct acatattgca gaagagtaca tttgaagtgc
     5041 ctgaaaacca gatgactatc ttaaagacca cttctgagga atgcagagat gctgatcttc
     5101 atgtcataat gaatgcccca tcgattggtc aggtagacag cagcaagcaa tttgaaggta
     5161 cagttgaaat taaacggaag tttgctggcc tgttgaaaaa tgactgtaac aaaagtgctt
     5221 ctggttattt aacagatgaa aatgaagtgg ggtttagggg cttttattct gctcatggca
     5281 caaaactgaa tgtttctact gaagctctgc aaaaagctgt gaaactgttt agtgatattg
     5341 agaatattag tgaggaaact tctgcagagg tacatccaat aagtttatct tcaagtaaat
     5401 gtcatgattc tgttgtttca atgtttaaga tagaaaatca taatgataaa actgtaagtg
     5461 aaaaaaataa taaatgccaa ctgatattac aaaataatat tgaaatgact actggcactt
     5521 ttgttgaaga aattactgaa aattacaaga gaaatactga aaatgaagat aacaaatata
     5581 ctgctgccag tagaaattct cataacttag aatttgatgg cagtgattca agtaaaaatg
     5641 atactgtttg tattcataaa gatgaaacgg acttgctatt tactgatcag cacaacatat
     5701 gtcttaaatt atctggccag tttatgaagg agggaaacac tcagattaaa gaagatttgt
     5761 cagatttaac ttttttggaa gttgcgaaag ctcaagaagc atgtcatggt aatacttcaa
     5821 ataaagaaca gttaactgct actaaaacgg agcaaaatat aaaagatttt gagacttctg
     5881 atacattttt tcagactgca agtgggaaaa atattagtgt cgccaaagag tcatttaata
     5941 aaattgtaaa tttctttgat cagaaaccag aagaattgca taacttttcc ttaaattctg
     6001 aattacattc tgacataaga aagaacaaaa tggacattct aagttatgag gaaacagaca
     6061 tagttaaaca caaaatactg aaagaaagtg tcccagttgg tactggaaat caactagtga
     6121 ccttccaggg acaacccgaa cgtgatgaaa agatcaaaga acctactctg ttgggttttc
     6181 atacagctag cgggaaaaaa gttaaaattg caaaggaatc tttggacaaa gtgaaaaacc
     6241 tttttgatga aaaagagcaa ggtactagtg aaatcaccag ttttagccat caatgggcaa
     6301 agaccctaaa gtacagagag gcctgtaaag accttgaatt agcatgtgag accattgaga
     6361 tcacagctgc cccaaagtgt aaagaaatgc agaattctct caataatgat aaaaaccttg
     6421 tttctattga gactgtggtg ccacctaagc tcttaagtga taatttatgt agacaaactg
     6481 aaaatctcaa aacatcaaaa agtatctttt tgaaagttaa agtacatgaa aatgtagaaa
     6541 aagaaacagc aaaaagtcct gcaacttgtt acacaaatca gtccccttat tcagtcattg
     6601 aaaattcagc cttagctttt tacacaagtt gtagtagaaa aacttctgtg agtcagactt
     6661 cattacttga agcaaaaaaa tggcttagag aaggaatatt tgatggtcaa ccagaaagaa
     6721 taaatactgc agattatgta ggaaattatt tgtatgaaaa taattcaaac agtactatag
     6781 ctgaaaatga caaaaatcat ctctccgaaa aacaagatac ttatttaagt aacagtagca
     6841 tgtctaacag ctattcctac cattctgatg aggtatataa tgattcagga tatctctcaa
     6901 aaaataaact tgattctggt attgagccag tattgaagaa tgttgaagat caaaaaaaca
     6961 ctagtttttc caaagtaata tccaatgtaa aagatgcaaa tgcataccca caaactgtaa
     7021 atgaagatat ttgcgttgag gaacttgtga ctagctcttc accctgcaaa aataaaaatg
     7081 cagccattaa attgtccata tctaatagta ataattttga ggtagggcca cctgcattta
     7141 ggatagccag tggtaaaatc gtttgtgttt cacatgaaac aattaaaaaa gtgaaagaca
     7201 tatttacaga cagtttcagt aaagtaatta aggaaaacaa cgagaataaa tcaaaaattt
     7261 gccaaacgaa aattatggca ggttgttacg aggcattgga tgattcagag gatattcttc
     7321 ataactctct agataatgat gaatgtagca cgcattcaca taaggttttt gctgacattc
     7381 agagtgaaga aattttacaa cataaccaaa atatgtctgg attggagaaa gtttctaaaa
     7441 tatcaccttg tgatgttagt ttggaaactt cagatatatg taaatgtagt atagggaagc
     7501 ttcataagtc agtctcatct gcaaatactt gtgggatttt tagcacagca agtggaaaat
     7561 ctgtccaggt atcagatgct tcattacaaa acgcaagaca agtgttttct gaaatagaag
     7621 atagtaccaa gcaagtcttt tccaaagtat tgtttaaaag taacgaacat tcagaccagc
     7681 tcacaagaga agaaaatact gctatacgta ctccagaaca tttaatatcc caaaaaggct
     7741 tttcatataa tgtggtaaat tcatctgctt tctctggatt tagtacagca agtggaaagc
     7801 aagtttccat tttagaaagt tccttacaca aagttaaggg agtgttagag gaatttgatt
     7861 taatcagaac tgagcatagt cttcactatt cacctacgtc tagacaaaat gtatcaaaaa
     7921 tacttcctcg tgttgataag agaaacccag agcactgtgt aaactcagaa atggaaaaaa
     7981 cctgcagtaa agaatttaaa ttatcaaata acttaaatgt tgaaggtggt tcttcagaaa
     8041 ataatcactc tattaaagtt tctccatatc tctctcaatt tcaacaagac aaacaacagt
     8101 tggtattagg aaccaaagtc tcacttgttg agaacattca tgttttggga aaagaacagg
     8161 cttcacctaa aaacgtaaaa atggaaattg gtaaaactga aactttttct gatgttcctg
     8221 tgaaaacaaa tatagaagtt tgttctactt actccaaaga ttcagaaaac tactttgaaa
     8281 cagaagcagt agaaattgct aaagctttta tggaagatga tgaactgaca gattctaaac
     8341 tgccaagtca tgccacacat tctcttttta catgtcccga aaatgaggaa atggttttgt
     8401 caaattcaag aattggaaaa agaagaggag agccccttat cttagtggga gaaccctcaa
     8461 tcaaaagaaa cttattaaat gaatttgaca ggataataga aaatcaagaa aaatccttaa
     8521 aggcttcaaa aagcactcca gatggcacaa taaaagatcg aagattgttt atgcatcatg
     8581 tttctttaga gccgattacc tgtgtaccct ttcgcacaac taaggaacgt caagagatac
     8641 agaatccaaa ttttaccgca cctggtcaag aatttctgtc taaatctcat ttgtatgaac
     8701 atctgacttt ggaaaaatct tcaagcaatt tagcagtttc aggacatcca ttttatcaag
     8761 tttctgctac aagaaatgaa aaaatgagac acttgattac tacaggcaga ccaaccaaag
     8821 tctttgttcc accttttaaa actaaatcac attttcacag agttgaacag tgtgttagga
     8881 atattaactt ggaggaaaac agacaaaagc aaaacattga tggacatggc tctgatgata
     8941 gtaaaaataa gattaatgac aatgagattc atcagtttaa caaaaacaac tccaatcaag
     9001 cagcagctgt aactttcaca aagtgtgaag aagaaccttt agatttaatt acaagtcttc
     9061 agaatgccag agatatacag gatatgcgaa ttaagaagaa acaaaggcaa cgcgtctttc
     9121 cacagccagg cagtctgtat cttgcaaaaa catccactct gcctcgaatc tctctgaaag
     9181 cagcagtagg aggccaagtt ccctctgcgt gttctcataa acagctgtat acgtatggcg
     9241 tttctaaaca ttgcataaaa attaacagca aaaatgcaga gtcttttcag tttcacactg
     9301 aagattattt tggtaaggaa agtttatgga ctggaaaagg aatacagttg gctgatggtg
     9361 gatggctcat accctccaat gatggaaagg ctggaaaaga agaattttat agggctctgt
     9421 gtgacactcc aggtgtggat ccaaagctta tttctagaat ttgggtttat aatcactata
     9481 gatggatcat atggaaactg gcagctatgg aatgtgcctt tcctaaggaa tttgctaata
     9541 gatgcctaag cccagaaagg gtgcttcttc aactaaaata cagatatgat acggaaattg
     9601 atagaagcag aagatcggct ataaaaaaga taatggaaag ggatgacaca gctgcaaaaa
     9661 cacttgttct ctgtgtttct gacataattt cattgagcgc aaatatatct gaaacttcta
     9721 gcaataaaac tagtagtgca gatacccaaa aagtggccat tattgaactt acagatgggt
     9781 ggtatgctgt taaggcccag ttagatcctc ccctcttagc tgtcttaaag aatggcagac
     9841 tgacagttgg tcagaagatt attcttcatg gagcagaact ggtgggctct cctgatgcct
     9901 gtacacctct tgaagcccca gaatctctta tgttaaagat ttctgctaac agtactcggc
     9961 ctgctcgctg gtataccaaa cttggattct ttcctgaccc tagacctttt cctctgccct
    10021 tatcatcgct tttcagtgat ggaggaaatg ttggttgtgt tgatgtaatt attcaaagag
    10081 cataccctat acagtggatg gagaagacat catctggatt atacatattt cgcaatgaaa
    10141 gagaggaaga aaaggaagca gcaaaatatg tggaggccca acaaaagaga ctagaagcct
    10201 tattcactaa aattcaggag gaatttgaag aacatgaaga aaacacaaca aaaccatatt
    10261 taccatcacg tgcactaaca agacagcaag ttcgtgcttt gcaagatggt gcagagcttt
    10321 atgaagcagt gaagaatgca gcagacccag cttaccttga gggttatttc agtgaagagc
    10381 agttaagagc cttgaataat cacaggcaaa tgttgaatga taagaaacaa gctcagatcc
    10441 agttggaaat taggaaggcc atggaatctg ctgaacaaaa ggaacaaggt ttatcaaggg
    10501 atgtcacaac cgtgtggaag ttgcgtattg taagctattc aaaaaaagaa aaagattcag
    10561 ttatactgag tatttggcgt ccatcatcag atttatattc tctgttaaca gaaggaaaga
    10621 gatacagaat ttatcatctt gcaacttcaa aatctaaaag taaatctgaa agagctaaca
    10681 tacagttagc agcgacaaaa aaaactcagt atcaacaact accggtttca gatgaaattt
    10741 tatttcagat ttaccagcca cgggagcccc ttcacttcag caaattttta gatccagact
    10801 ttcagccatc ttgttctgag gtggacctaa taggatttgt cgtttctgtt gtgaaaaaaa
    10861 caggacttgc ccctttcgtc tatttgtcag acgaatgtta caatttactg gcaataaagt
    10921 tttggataga ccttaatgag gacattatta agcctcatat gttaattgct gcaagcaacc
    10981 tccagtggcg accagaatcc aaatcaggcc ttcttacttt atttgctgga gatttttctg
    11041 tgttttctgc tagtccaaaa gagggccact ttcaagagac attcaacaaa atgaaaaata
    11101 ctgttgagaa tattgacata ctttgcaatg aagcagaaaa caagcttatg catatactgc
    11161 atgcaaatga tcccaagtgg tccaccccaa ctaaagactg tacttcaggg ccgtacactg
    11221 ctcaaatcat tcctggtaca ggaaacaagc ttctgatgtc ttctcctaat tgtgagatat
    11281 attatcaaag tcctttatca ctttgtatgg ccaaaaggaa gtctgtttcc acacctgtct
    11341 cagcccagat gacttcaaag tcttgtaaag gggagaaaga gattgatgac caaaagaact
    11401 gcaaaaagag aagagccttg gatttcttga gtagactgcc tttacctcca cctgttagtc
    11461 ccatttgtac atttgtttct ccggctgcac agaaggcatt tcagccacca aggagttgtg
    11521 gcaccaaata cgaaacaccc ataaagaaaa aagaactgaa ttctcctcag atgactccat
    11581 ttaaaaaatt caatgaaatt tctcttttgg aaagtaattc aatagctgac gaagaacttg
    11641 cattgataaa tacccaagct cttttgtctg gttcaacagg agaaaaacaa tttatatctg
    11701 tcagtgaatc cactaggact gctcccacca gttcagaaga ttatctcaga ctgaaacgac
    11761 gttgtactac atctctgatc aaagaacagg agagttccca ggccagtacg gaagaatgtg
    11821 agaaaaataa gcaggacaca attacaacta aaaaatatat ctaagcattt gcaaaggcga
    11881 caataaatta ttgacgctta acctttccag tttataagac tggaatataa tttcaaacca
    11941 cacattagta cttatgttgc acaatgagaa aagaaattag tttcaaattt acctcagcgt
    12001 ttgtgtatcg ggcaaaaatc gttttgcccg attccgtatt ggtatacttt tgcttcagtt
    12061 gcatatctta aaactaaatg taatttatta actaatcaag aaaaacatct ttggctgcgg
    12121 tcgagcatgc atctagaggg ccctattcta tagtgtcacc taaatgctag agctcgctga
    12181 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
    12241 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
    12301 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
    12361 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
    12421 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
    12481 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
    12541 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
    12601 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
    12661 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
    12721 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
    12781 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
    12841 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
    12901 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
    12961 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
    13021 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
    13081 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
    13141 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
    13201 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
    13261 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
    13321 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
    13381 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
    13441 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
    13501 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
    13561 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
    13621 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
    13681 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
    13741 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
    13801 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
    13861 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
    13921 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
    13981 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
    14041 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
    14101 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
    14161 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
    14221 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
    14281 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    14341 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
    14401 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
    14461 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
    14521 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
    14581 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
    14641 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
    14701 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
    14761 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
    14821 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    14881 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    14941 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    15001 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    15061 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    15121 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    15181 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    15241 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
    15301 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    15361 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    15421 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    15481 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    15541 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    15601 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    15661 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    15721 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    15781 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    15841 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    15901 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    15961 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    16021 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    16081 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    16141 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    16201 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    16261 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    16321 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    16381 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    16441 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    16501 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    16561 gcgcacattt ccccgaaaag tgccacctga cgtc
//
