LOCUS       sequence-26754-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Nam HS, Benezra R
  TITLE     High levels of Id1 expression define B1 type adult neural stem 
            cells.
  JOURNAL   Cell Stem Cell. 2009 Nov 6. 5(5):515-26.
  PUBMED    19896442
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2009 Nov 6. 5(5):515-26."
COMMENT     Sequence Label: H2B-Venus
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..83
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        64..82
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             108..485
                     /codon_start=1
                     /gene="HIST1H2BB"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPSKSAPAPKKGSKKAITKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSSK"
     regulatory      498..507
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(549..570)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gtggagnatt agcagagctc tctggctact agagaaccca ctgccttact ggcttatcga
       61 aattaatacg actcactata gggagaccca agcttggtac cgccaccatg cctgaaccct
      121 ctaagtctgc tccagcccct aaaaagggtt ctaagaaggc tatcactaag gcgcagaaga
      181 aggatggtaa gaagcgtaag cgcagccgca aggagagcta ttctatctat gtgtacaagg
      241 ttctgaagca ggtccacccc gacaccggca tctcatccaa ggccatgggg atcatgaact
      301 ccttcgtcaa cgacatcttc gagcgcatcg cgggcgaggc ttctcgcctg gctcactaca
      361 ataagcgctc gaccatcacc tccagggaga ttcagacggc tgtgcgcctg ctgctgcctg
      421 gggagctggc taagcatgct gtgtccgagg gcactaaggc agttaccaag tacactagct
      481 ctaaggatcc accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
      541 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
      601 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc accaccggca
      661 agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcctg cagtgcttcg
      721 cccgctaccc cgaccacatg aagcagcaca ttntcngnnn tncnttaggg nnnaggttac
      781 ttnanngccc ntttttntnt ttnaggacan gcaactacag accgcgcnaa gtganttcga
      841 ggcgaccc
//
