LOCUS       sequence-267557-        6464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression of D. melanogaster (Dm) cytochrome with 
            C-terminal V5 and His tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6464)
  AUTHORS   Ozturk N, Selby CP, Song SH, Ye R, Tan C, Kao YT, Zhong D, Sancar A
  TITLE     Comparative photochemistry of animal type 1 and type 4 
            cryptochromes.
  JOURNAL   Biochemistry. 2009 Sep 15;48(36):8585-93. doi: 10.1021/bi901043s.
  PUBMED    19663499
REFERENCE   2  (bases 1 to 6464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochemistry"; date: "2009-09-15- 15"; volume: "48"; issue: "36"; 
            pages: "8585-93"
COMMENT     Sequence Label: pFast-dCry-V5His
FEATURES             Location/Qualifiers
     source          1..6464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(251..784)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(954..959,960..976,977..982))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1334..1425
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1350..1367
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             3152..3193
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3203..3220
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3403..3537
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3440..3459)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3494..3513
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3566..3731
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3915..4369
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4002..4021)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4211..4232
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4395..4499
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4500..4568,4569..5360)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4718..4737)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5531..6119
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6020..6039
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6273..6290
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  join(6424..6464,1..184)
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
ORIGIN
        1 taaactatga caataaagtc ttaaactaga cagaatagtt gtaaactgaa atcagtccag
       61 ttatgctgtg aaaaagcata ctggactttt gttatggcta aagcaaactc ttcattttct
      121 gaagtgcaaa ttgcccgtcg tattaaagag gggcgtggcc aagggcatgg taaagactat
      181 attcgcggcg ttgtgacaat ttaccgaaca actccgcggc cgggaagccg atctcggctt
      241 gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact tcttcccgta
      301 tgcccaactt tgtatagaga gccactgcgg gatcgtcacc gtaatctgct tgcacgtaga
      361 tcacataagc accaagcgcg ttggcctcat gcttgaggag attgatgagc gcggtggcaa
      421 tgccctgcct ccggtgctcg ccggagactg cgagatcata gatatagatc tcactacgcg
      481 gctgctcaaa cttgggcaga acgtaagccg cgagagcgcc aacaaccgct tcttggtcga
      541 aggcagcaag cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa tcggagtccg
      601 gctgatgttg ggagtaggtg gctacgtctc cgaactcacg accgaaaaga tcaagagcag
      661 cccgcatgga tttgacttgg tcagggccga gcctacatgt gcgaatgatg cccatacttg
      721 agccacctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctccata
      781 acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact
      841 gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc
      901 gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac
      961 gaaccgaaca ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc
     1021 acccggcaac cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc
     1081 gcaaggtttc ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca
     1141 aggtgctgtg cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc
     1201 gcttgccggt ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg
     1261 agcatcgttt gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc
     1321 ggaatattaa tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta
     1381 ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca
     1441 taccgtccca ccatcgggcg cggatcccgg tccgaagcgc gcggaattca ccatggccac
     1501 gcgaggggcg aatgtgattt ggtttcgcca tggattgcgc ctccatgata atcccgctct
     1561 attggccgcc ctcgccgata aggatcaggg tatagcccta attcccgttt tcatattcga
     1621 tggagagagt gcaggtacca agaatgtggg ttacaatcgg atgcgtttcc tcctggactc
     1681 gttgcaggac atcgatgatc agctacaggc ggcaactgat ggacgtggac gcctcctggt
     1741 cttcgagggc gaaccggctt atatcttccg ccggctacat gagcaagtgc gtctgcacag
     1801 gatttgcata gagcaggact gcgagccaat ttggaatgag cgcgatgaaa gcatccgttc
     1861 tctatgtcgg gagctgaata tcgactttgt cgagaaggta tcacacacgc tttgggatcc
     1921 gcaattggtg attgagacca atggtggcat tccaccgctg acctaccaaa tgttcctgca
     1981 cacggtgcaa attattgggc ttccaccgcg tcccaccgcc gatgctcgac tagaagacgc
     2041 cacctttgtc gagctggacc ccgagttctg ccgaagtctt aagttgttcg agcagctgcc
     2101 cacgccggag cacttcaatg tgtatggaga caacatgggc ttcctggcca agattaactg
     2161 gcgcggcgga gaaacacagg ccttacttct gttggatgag cgtcttaaag tggagcagca
     2221 tgcgtttgag cgtggatttt atctgcccaa ccaggcactg cccaatatcc acgactcgcc
     2281 aaaatcgatg agcgcccatc tgcgctttgg ttgcctttcg gtacgtcgct tctactggag
     2341 cgtccacgat ctcttcaaga atgtccagtt gcgcgcctgt gtgcggggcg ttcagatgac
     2401 tggcggcgcg cacatcacgg gacagttgat ctggcgagag tacttctaca ccatgtcggt
     2461 gaacaatcca aactacgatc gcatggaggg caatgacatc tgcctgagca tcccgtgggc
     2521 taagccgaac gaaaatctcc tgcagagctg gcgtttaggc caaacgggat tcccgctcat
     2581 cgacggcgcc atgcgacaac tcctggccga gggatggctc caccatacgc tgcgcaacac
     2641 cgtggccacc tttctcacgc gcggcggttt gtggcagagc tgggagcatg gactgcagca
     2701 ctttctgaag tatctgctgg atgcggattg gtcggtctgc gctggcaact ggatgtgggt
     2761 atccagctcg gcgtttgaaa ggctgctgga ctcctccctg gtcacctgcc cggtggcatt
     2821 ggccaagcga cttgatccgg atggcaccta catcaagcag tacgtcccgg agttgatgaa
     2881 tgtgcccaag gaatttgttc acgagccctg gcgaatgtct gccgagcagc aggagcagta
     2941 cgagtgcctg atcggagtcc attatccgga gcggatcatt gatttgtcca tggccgtaaa
     3001 gcgaaacatg ctggccatga agtctctccg aaattcgctg atcaccccgc cgccgcattg
     3061 ccgaccatcc aacgaggagg aagtgcgtca gttcttctgg ctggccgacg tggtggtttg
     3121 gcggccgctc gagtctagag ggcccttcga aggtaagcct atccctaacc ctctcctcgg
     3181 tctcgattct acgcgtaccg gtcatcatca ccatcaccat tgagtttggc cgctttcgaa
     3241 tctagagcct gcagtctcga ggcatgcggt accaagcttg tcgagaagta ctagaggatc
     3301 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     3361 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     3421 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     3481 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctga
     3541 tcactgcttg agcctaggag atccgaacca gataagtgaa atctagttcc aaactatttt
     3601 gtcattttta attttcgtat tagcttacga cgctacaccc agttcccatc tattttgtca
     3661 ctcttcccta aataatcctt aaaaactcca tttccacccc tcccagttcc caactatttt
     3721 gtccgcccac agcggggcat ttttcttcct gttatgtttt taatcaaaca tcctgccaac
     3781 tccatgtgac aaaccgtcat cttcggctac tttttctctg tcacagaatg aaaatttttc
     3841 tgtcatctct tcgttattaa tgtttgtaat tgactgaata tcaacgctta tttgcagcct
     3901 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     3961 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     4021 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     4081 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     4141 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     4201 gttcttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     4261 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4321 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     4381 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     4441 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     4501 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     4561 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     4621 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     4681 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     4741 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     4801 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     4861 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     4921 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     4981 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     5041 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     5101 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     5161 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     5221 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     5281 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     5341 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     5401 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     5461 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5521 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5581 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5641 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     5701 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5761 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5821 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5881 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     5941 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6001 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6061 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6121 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     6181 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     6241 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     6301 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatagacc
     6361 agccgcgtaa cctggcaaaa tcggttacgg ttgagtaata aatggatgcc ctgcgtaagc
     6421 gggtgtgggc ggacaataaa gtcttaaact gaacaaaata gatc
//
