LOCUS       sequence-267562-       15448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15448)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX006
FEATURES             Location/Qualifiers
     source          1..15448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(50..67)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(50..66)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(59..81)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(206..225)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     416..437
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    684..708
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(2304..3452)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(3751..4545)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  4737..5504
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      6127..6838
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(6965..7336)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            7369..7478
                     /note="incP origin of transfer"
     primer_bind     7701..7720
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7954..7971
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(8159..8809)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    9274..9298
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        9397..9742
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    10369..10545
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        11048..11393
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    11415..11439
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(12400..12424)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             12453..12542
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      12576..12828
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(13257..13509)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(13977..15002)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(13977..15002)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(15012..15356)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
ORIGIN
        1 gtcgacgcgt cgacgcgtcg acgcgtcgac gcgtcctaga agctaattca ctggccgtcg
       61 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
      121 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
      181 agttgcgcag cctgaatggc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
      241 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
      301 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
      361 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
      421 ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa
      481 gggattttgc cgatttcgga accaccatca aacaggattt tcgcctgctg gggcaaacca
      541 gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc
      601 ccgtctcact ggtgaaaaga aaaaccaccc cagtacatta aaaacgtccg caatgtgtta
      661 ttaagttgtc taagcgtcaa tttgtttaca ccacaatata tcctgccacc agccagccaa
      721 cagctccccg accggcagct cggcacaaaa tcaccactcg atacaggcag cccatcagtc
      781 cgggacggcg tcagcgggag agccgttgta aggcggcaga ctttgctcat gttaccgatg
      841 ctattcggaa gaacggcaac taagctgccg ggtttgaaac acggatgatc tcgcggaggg
      901 tagcatgttg attgtaacga tgacagagcg ttgctgcctg tgatcaaata tcatctccct
      961 cgcagagatc cgaattatca gccttcttat tcatttctcg cttaaccgtg acaggctgtc
     1021 gatcttgaga actatgccga cataatagga aatcgctgga taaagccgct gaggaagctg
     1081 agtggcgcta tttctttaga agtgaacgtt gacgatatca actcccctat ccattgctca
     1141 ccgaatggta caggtcgggg acccgaagtt ccgactgtcg gcctgatgca tccccggctg
     1201 atcgacccca gatctggggc tgagaaagcc cagtaaggaa acaactgtag gttcgagtcg
     1261 cgagatcccc cggaaccaaa ggaagtaggt taaacccgct ccgatcaggc cgagccacgc
     1321 caggccgaga acattggttc ctgtaggcat cgggattggc ggatcaaaca ctaaagctac
     1381 tggaacgagc agaagtcctc cggccgccag ttgccaggcg gtaaaggtga gcagaggcac
     1441 gggaggttgc cacttgcggg tcagcacggt tccgaacgcc atggaaaccg cccccgccag
     1501 gcccgctgcg acgccgacag gatctagcgc tgcgtttggt gtcaacacca acagcgccac
     1561 gcccgcagtt ccgcaaatag cccccaggac cgccatcaat cgtatcgggc tacctagcag
     1621 agcggcagag atgaacacga ccatcagcgg ctgcacagcg cctaccgtcg ccgcgacccc
     1681 gcccggcagg cggtagaccg aaataaacaa caagctccag aatagcgaaa tattaagtgc
     1741 gccgaggatg aagatgcgca tccaccagat tcccgttgga atctgtcgga cgatcatcac
     1801 gagcaataaa cccgccggca acgcccgcag cagcataccg gcgacccctc ggcctcgctg
     1861 ttcgggctcc acgaaaacgc cggacagatg cgccttgtga gcgtccttgg ggccgtcctc
     1921 ctgtttgaag accgacagcc caatgatctc gccgtcgatg taggcgccga atgccacggc
     1981 atctcgcaac cgttcagcga acgcctccat gggctttttc tcctcgtgct cgtaaacgga
     2041 cccgaacatc tctggagctt tcttcagggc cgacaatcgg atctcgcgga aatcctgcac
     2101 gtcggccgct ccaagccgtc gaatctgagc cttaatcaca attgtcaatt ttaatcctct
     2161 gtttatcggc agttcgtaga gcgcgccgtg cgtcccgagc gatactgagc gaagcaagtg
     2221 cgtcgagcag tgcccgcttg ttcctgaaat gccagtaaag cgctggctgc tgaaccccca
     2281 gccggaactg accccacaag gccctagcgt ttgcaatgca ccaggtcatc attgacccag
     2341 gcgtgttcca ccaggccgct gcctcgcaac tcttcgcagg cttcgccgac ctgctcgcgc
     2401 cacttcttca cgcgggtgga atccgatccg cacatgaggc ggaaggtttc cagcttgagc
     2461 gggtacggct cccggtgcga gctgaaatag tcgaacatcc gtcgggccgt cggcgacagc
     2521 ttgcggtact tctcccatat gaatttcgtg tagtggtcgc cagcaaacag cacgacgatt
     2581 tcctcgtcga tcaggacctg gcaacgggac gttttcttgc cacggtccag gacgcggaag
     2641 cggtgcagca gcgacaccga ttccaggtgc ccaacgcggt cggacgtgaa gcccatcgcc
     2701 gtcgcctgta ggcgcgacag gcattcctcg gccttcgtgt aataccggcc attgatcgac
     2761 cagcccaggt cctggcaaag ctcgtagaac gtgaaggtga tcggctcgcc gataggggtg
     2821 cgcttcgcgt actccaacac ctgctgccac accagttcgt catcgtcggc ccgcagctcg
     2881 acgccggtgt aggtgatctt cacgtccttg ttgacgtgga aaatgacctt gttttgcagc
     2941 gcctcgcgcg ggattttctt gttgcgcgtg gtgaacaggg cagagcgggc cgtgtcgttt
     3001 ggcatcgctc gcatcgtgtc cggccacggc gcaatatcga acaaggaaag ctgcatttcc
     3061 ttgatctgct gcttcgtgtg tttcagcaac gcggcctgct tggcctcgct gacctgtttt
     3121 gccaggtcct cgccggcggt ttttcgcttc ttggtcgtca tagttcctcg cgtgtcgatg
     3181 gtcatcgact tcgccaaacc tgccgcctcc tgttcgagac gacgcgaacg ctccacggcg
     3241 gccgatggcg cgggcagggc agggggagcc agttgcacgc tgtcgcgctc gatcttggcc
     3301 gtagcttgct ggaccatcga gccgacggac tggaaggttt cgcggggcgc acgcatgacg
     3361 gtgcggcttg cgatggtttc ggcatcctcg gcggaaaacc ccgcgtcgat cagttcttgc
     3421 ctgtatgcct tccggtcaaa cgtccgattc attcaccctc cttgcgggat tgccccgact
     3481 cacgccgggg caatgtgccc ttattcctga tttgacccgc ctggtgcctt ggtgtccaga
     3541 taatccacct tatcggcaat gaagtcggtc ccgtagaccg tctggccgtc cttctcgtac
     3601 ttggtattcc gaatcttgcc ctgcacgaat accagcgacc ccttgcccaa atacttgccg
     3661 tgggcctcgg cctgagagcc aaaacacttg atgcggaaga agtcggtgcg ctcctgcttg
     3721 tcgccggcat cgttgcgcca catctaggta ctaaaacaat tcatccagta aaatataata
     3781 ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct cgacatactg
     3841 ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat accacttgtc
     3901 cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt tcacaaagat
     3961 gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt
     4021 taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcttcttccc agttttcgca
     4081 atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc ggctgtctaa
     4141 gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga tgcactccgc
     4201 atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg agcaaaggac
     4261 gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa agtgcaggac
     4321 ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc gttccacatc
     4381 ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt cattttctcc
     4441 caccagctta tataccttag caggagacat tccttccgta tcttttacgc agcggtattt
     4501 ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccatttatt atttccttcc
     4561 tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac gaactccaat
     4621 tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc aaagttgttt
     4681 tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccaca attatgggtg
     4741 atgctgccaa cttactgatt tagtgtatga tggtgttttt gaggtgctcc agtggcttct
     4801 gtttctatca gctgtccctc ctgttcagct actgacgggg tggtgcgtaa cggcaaaagc
     4861 accgccggac atcagcgcta tctctgctct cactgccgta aaacatggca actgcagttc
     4921 acttacaccg cttctcaacc cggtacgcac cagaaaatca ttgatatggc catgaatggc
     4981 gttggatgcc gggcaacagc ccgcattatg ggcgttggcc tcaacacgat tttacgtcac
     5041 ttaaaaaact caggccgcag tcggtaacct cgcgcataca gccgggcagt gacgtcatcg
     5101 tctgcgcgga aatggacgaa cagtggggct atgtcggggc taaatcgcgc cagcgctggc
     5161 tgttttacgc gtatgacagt ctccggaaga cggttgttgc gcacgtattc ggtgaacgca
     5221 ctatggcgac gctggggcgt cttatgagcc tgctgtcacc ctttgacgtg gtgatatgga
     5281 tgacggatgg ctggccgctg tatgaatccc gcctgaaggg aaagctgcac gtaatcagca
     5341 agcgatatac gcagcgaatt gagcggcata acctgaatct gaggcagcac ctggcacggc
     5401 tgggacggaa gtcgctgtcg ttctcaaaat cggtggagct gcatgacaaa gtcatcgggc
     5461 attatctgaa cataaaacac tatcaataag ttggagtcat tacccaatta tgatagaatt
     5521 tacaagctat aaggttattg tcctgggttt caagcattag tccatgcaag tttttatgct
     5581 ttgcccattc tatagatata ttgataagcg cgctgcctat gccttgcccc ctgaaatcct
     5641 tacatacggc gatatcttct atataaaaga tatattatct tatcagtatt gtcaatatat
     5701 tcaaggcaat ctgcctcctc atcctcttca tcctcttcgt cttggtagct ttttaaatat
     5761 ggcgcttcat agagtaattc tgtaaaggtc caattctcgt tttcatacct cggtataatc
     5821 ttacctatca cctcaaatgg ttcgctgggt ttatcgcacc cccgaacacg agcacggcac
     5881 ccgcgaccac tatgccaaga atgcccaagg taaaaattgc cggccccgcc atgaagtccg
     5941 tgaatgcccc gacggccgaa gtgaagggca ggccgccacc caggccgccg ccctcactgc
     6001 ccggcacctg gtcgctgaat gtcgatgcca gcacctgcgg cacgtcaatg cttccgggcg
     6061 tcgcgctcgg gctgatcgcc catcccgtta ctgccccgat cccggcaatg gcaaggactg
     6121 ccagcgctgc catttttggg gtgaggccgt tcgcggccga ggggcgcagc ccctgggggg
     6181 atgggaggcc cgcgttagcg ggccgggagg gttcgagaag ggggggcacc ccccttcggc
     6241 gtgcgcggtc acgcgcacag ggcgcagccc tggttaaaaa caaggtttat aaatattggt
     6301 ttaaaagcag gttaaaagac aggttagcgg tggccgaaaa acgggcggaa acccttgcaa
     6361 atgctggatt ttctgcctgt ggacagcccc tcaaatgtca ataggtgcgc ccctcatctg
     6421 tcagcactct gcccctcaag tgtcaaggat cgcgcccctc atctgtcagt agtcgcgccc
     6481 ctcaagtgtc aataccgcag ggcacttatc cccaggcttg tccacatcat ctgtgggaaa
     6541 ctcgcgtaaa atcaggcgtt ttcgccgatt tgcgaggctg gccagctcca cgtcgccggc
     6601 cgaaatcgag cctgcccctc atctgtcaac gccgcgccgg gtgagtcggc ccctcaagtg
     6661 tcaacgtccg cccctcatct gtcagtgagg gccaagtttt ccgcgaggta tccacaacgc
     6721 cggcggccgc ggtgtctcgc acacggcttc gacggcgttt ctggcgcgtt tgcagggcca
     6781 tagacggccg ccagcccagc ggcgagggca accagcccgg tgagcgtcgg aaaggcgctc
     6841 ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc gcgaagtcgc tcttcttgat
     6901 ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc cggccgtttt agcggctaaa
     6961 aaagtcatgg ctctgccctc gggcggacca cgcccatcat gaccttgcca agctcgtcct
     7021 gcttctcttc gatcttcgcc agcagggcga ggatcgtggc atcaccgaac cgcgccgtgc
     7081 gcgggtcgtc ggtgagccag agtttcagca ggccgcccag gcggcccagg tcgccattga
     7141 tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc cgtgattttg tagccctggc
     7201 cgacggccag caggtaggcc gacaggctca tgccggccgc cgccgccttt tcctcaatcg
     7261 ctcttcgttc gtctggaagg cagtacacct tgataggtgg gctgcccttc ctggttggct
     7321 tggtttcatc agccatccgc ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc
     7381 gcagagcagg attcccgttg agcaccgcca ggtgcgaata agggacagtg aagaaggaac
     7441 acccgctcgc gggtgggcct acttcaccta tcctgcccgg ctgacgccgt tggatacacc
     7501 aaggaaagtc tacacgaacc ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat
     7561 ataccgaaaa aatcgctata atgaccccga agcagggtta tgcagcggaa aagcgccacg
     7621 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     7681 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     7741 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     7801 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     7861 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     7921 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     7981 gagcgccaga aggccgccag agaggccgag cgcggccgtg aggcttggac gctagggcag
     8041 ggcatgaaaa agcccgtagc gggctgctac gggcgtctga cgcggtggaa agggggaggg
     8101 gatgttgtct acatggctct gctgtagtga gtgggttgcg ctccggcagc ggtcctgatc
     8161 aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac gagcctcctt ttcgccaatc
     8221 catcgacaat caccgcgagt ccctgctcga acgctgcgtc cggaccggct tcgtcgaagg
     8281 cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg ttcaacggtg ccgccgcgct
     8341 cgccggcatc gctgtcgccg gcctgctcct caagcacggc cccaacagtg aagtagctga
     8401 ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc cgcctcgcag aggaagcgaa
     8461 gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg cgtgccggca tggatgcgcg
     8521 cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg ggcattcccg atcagaaatg
     8581 agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg attctccgcc agcatggctt
     8641 cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg ccagtaaagc gccggctgct
     8701 gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc gtctacgccg acctcgttca
     8761 acaggtccag ggcggcacgg atcactgtat tcggctgcaa ctttgtcatg cttgacactt
     8821 tatcactgat aaacataata tgtccaccaa cttatcagtg ataaagaatc cgcgcgttca
     8881 atcggaccag cggaggctgg tccggaggcc agacgtgaaa cccaacatac ccctgatcgt
     8941 aattctgagc actgtcgcgc tcgacgctgt cggcatcggc ctgattatgc cggtgctgcc
     9001 gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc gcccactatg gcattctgct
     9061 ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg ctgggcgcgc tgtcggatcg
     9121 tttcgggcgg cggccaatct tgctcgtctc gctggccggc gccagatctg gggaaccctg
     9181 tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat
     9241 ccgattattc taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa
     9301 cactgatagt ttaaacgttg cgattaagaa ttcgagctcg gtacccccct actccaaaaa
     9361 tgtcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac aaagggtaat
     9421 ttcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatcg aaaggacagt
     9481 agaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg ctatcattca
     9541 agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
     9601 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgaca tctccactga
     9661 cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag
     9721 ttcatttcat ttggagagga cagcccaagc ttcgactcta gaggatcccc ttaaatcgat
     9781 atggaacgag ctatacaagg aaacgacgct agggaacaag ctaacagtga acgttgggat
     9841 ggaggatcag gaggtaccac ttctcccttc aaacttcctg acgaaagtcc gagttggact
     9901 gagtggcggc tacataacga tgagacgaat tcgaatcaag ataatcccct tggtttcaag
     9961 gaaagctggg gtttcgggaa agttgtattt aagagatatc tcagatacga caggacggaa
    10021 gcttcactgc acagagtcct tggatcttgg acgggagatt cggttaacta tgcagcatct
    10081 cgatttttcg gtttcgacca gatcggatgt acctatagta ttcggtttcg aggagttagt
    10141 atcaccgttt ctggagggtc tcgaactctt cagcatctct gtgagatggc aattcggtct
    10201 aagcaagaac tgctacagct tgccccaatc gaagtggaaa gtaatgtatc aagaggatgc
    10261 cctgaaggta ctcaaacctt cgaaaaagaa agcgagtaag tcgagggcga gctcgaattc
    10321 ggtacgctga aatcaccagt ctctctctac aaatctatct ctctctattt tctccataaa
    10381 taatgtgtga gtagtttccc gataagggaa attagggttc ttatagggtt tcgctcatgt
    10441 gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa
    10501 aatttctaat tcctaaaacc aaaatccagt actaaaatcc agatctccct gcaggtcccc
    10561 agattagcct tttcaatttc agaaagaatg ctaacccaca gatggttaga gaggcttacg
    10621 cagcaggtct catcaagacg atctacccga gcaataatct ccaggaaatc aaataccttc
    10681 ccaagaaggt taaagatgca gtcaaaagat tcaggactaa ctgcatcaag aacacagaga
    10741 aagatatatt tctcaagatc agaagtacta ttccagtatg gacgattcaa ggcttgcttc
    10801 acaaaccaag gcaagtaata gagattggag tctctaaaaa ggtagttccc actgaatcaa
    10861 aggccatgga gtcaaagatt caaatagagg acctaacaga actcgccgta aagactggcg
    10921 aacagttcat acagagtctc ttacgactca atgacaagaa gaaaatcttc gtcaacatgg
    10981 tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa
    11041 gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc
    11101 cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc
    11161 atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag
    11221 atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa
    11281 agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc
    11341 cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagaga acacggggga
    11401 ctctagagtt atcaacaagt ttgtacaaaa aagcaggctc caccactagt atggaatgtt
    11461 ttgatgaata ttttgcattg gagaaaatta gaaaccatct tcttggggat ttctccccaa
    11521 aaacattgaa atttggggcg gaattaacga gtaataagcc ttgcagtgat attatgacgg
    11581 agagttctag ctcttcagac ccttttccct ttcattttct ctccgattca ttcgattttg
    11641 aagctgattt ctttcaattt ggaacagaag tttcaaattc gagctcggaa tcggcaaatt
    11701 cagtgcatat atcaaacaat ttcatcaaac tcgaatcgga gccaacaatt tcaagctccg
    11761 aatacgagaa atctcaagta atcattgacc ttacatcacc taagttgagt aaagttagtg
    11821 atcggaaacc ttcattgaaa attgatttac cgccagtgaa gaaatttgag tggatcggtt
    11881 tcggtaattc aacggctcag tcaaattcaa ttgtttcggt gactatgcaa cagaagcgga
    11941 aaactgaaga gaagaggcat taccgaggag ttcgacagcg gccatgggga aaatacgcgg
    12001 cggagatccg agatcctaca cgccgtggtg ctagggtttg gcttgggaca tttaccaccg
    12061 cagttgaagc ggcgaaggct tacgatagag ccgcatttaa gatgcgtgga agtaaggcga
    12121 tattgaattt tccgttggag gtggggaaga acctgtcgta tgcaagctcc gccatggacg
    12181 gcggcgtgaa gcggcataga gaatcagaag aacgtgtaga agagaaggaa gtgaagaaag
    12241 tggagaggtg taaggaagag cagtcatggc cattaactcc gtcaagctgg aacttccttt
    12301 gtgaacagaa tactaacgga atgcttaatc tcccgccttt atcaccgtta tctcctcatc
    12361 cgccattggg ctgttcgcag cttattcaaa tccccgggga cccagctttc ttgtacaaag
    12421 tggttgataa cagcgggtta attaacatct tttacccata cgatgttcct gactatgcgg
    12481 gctatcccta tgacgtcccg gactatgcag gatcctatcc atatgacgtt ccagattacg
    12541 ctgctcagtg cagcgcttag agctcgaatt tccccgatcg ttcaaacatt tggcaataaa
    12601 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
    12661 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
    12721 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
    12781 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattagctt
    12841 catcaacgca agacatgcgc acgaccgtct gacaggagag gaatttccga cgagcacaga
    12901 aaggacttgc tcttggacgt aggcctattt ctcaggcaca tgtatcaagt gttcggacgt
    12961 gggttttcga tggtgtatca gccgccgcca actgggagat gaggaggctt tcttgggggg
    13021 cagtcagcag ttcatttcac aagacagagg aacttgtaag gagatgcact gatttatctt
    13081 ggcgcaaacc agcaggacga attagtggga atagcccgcg aatatctaag ttatgcctgt
    13141 cggcatgagc agaaacttcc aattcgaaac agtttggaga ggttgttttt gggcatacct
    13201 tttgttagtc agcctctcga ttgctcatcg tcattacaca gtaccgaagt ttgatcgatc
    13261 tagtaacata gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt
    13321 ttctatcgcg tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa
    13381 taacgtcatg cattacatgt taattattac atgcttaacg taattcaaca gaaattatat
    13441 gataatcatc gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt
    13501 tgaacgatct gcttcgacgc actccttctt tactccacca tctcgtcctt attgaaaacg
    13561 tgggtagcac caaaacgaat caagtcgctg gaactgaagt taccaatcac gctggatgat
    13621 ttgccagttg gattaatctt gcctttcccc gcatgaataa tattgatgaa tgcatgcgtg
    13681 aggggtattt cgattttggc aatagctgca attgccgcga catcctccaa cgagcataat
    13741 tcttcagaaa aatagcgatg ttccatgttg tcagggcatg catgatgcac gttatgaggt
    13801 gacggtgcta ggcagtattc cctcaaagtt tcatagtcag tatcatattc atcattgcat
    13861 tcctgcaaga gagaattgag acgcaatcca cacgctgcgg caaccttccg gcgttcgtgg
    13921 tctatttgct cttggacgtt gcaaacgtaa gtgttggatc ccggtcggca tctactctat
    13981 tcctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca
    14041 cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg
    14101 gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg
    14161 ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagc
    14221 cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata
    14281 caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac
    14341 atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg
    14401 gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc
    14461 agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag
    14521 tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg
    14581 attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc
    14641 gcatccatgg cctccgcgac cggctgcaga acagcgggca gttcggtttc aggcaggtct
    14701 tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaattcc
    14761 ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa
    14821 cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct
    14881 acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg
    14941 ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc
    15001 atatcggggt cgtcctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgcg
    15061 aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt
    15121 gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc
    15181 catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat
    15241 gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag
    15301 ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag
    15361 ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct
    15421 ccaccatgtt gacggatctc taggacgc
//
