LOCUS       sequence-267564-        5941 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression of Jetlag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Ozturk N, Selby CP, Zhong D, Sancar A
  TITLE     Mechanism of photosignaling by Drosophila cryptochrome: role of the 
            redox status of the flavin chromophore.
  JOURNAL   J Biol Chem. 2014 Feb 21;289(8):4634-42. doi: 
            10.1074/jbc.M113.542498. Epub 2013 Dec 30.
  PUBMED    24379403
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.542498"; journalName: "J Biol Chem"; date: 
            "2014-02-21- 21"; volume: "289"; issue: "8"; pages: "4634-42"
COMMENT     Sequence Label: pGex4T1-JET
FEATURES             Location/Qualifiers
     source          1..5941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(28..33,34..51,52..58)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    66..82
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     90..106
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     90..106
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             102..629
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGISLSTARCTNASGVR
                     QPSEAVVWLCRS"
     primer_bind     complement(122..139)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(122..138)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(131..153)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        join(731..736,737..752,753..759)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    767..783
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             806..1459
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     1417..1439
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             1466..1483
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(2539..2561)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2706..2724)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2792..2896
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2897..2965,2966..3757)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3115..3134)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3928..4516
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4417..4436
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4670..4687
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        4760..4837
                     /gene="lacI"
                     /label=lacI promoter
     CDS             4838..5920
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(4857..4876)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
       61 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacggatt
      121 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
      181 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
      241 gcccttccca acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg tttccggcac
      301 cagaagcggt gccggaaagc tggctggagt gcgatcttcc tgaggccgat actgtcgtcg
      361 tcccctcaaa ctggcagatg cacggttacg atgcgcccat ctacaccaac gtgacctatc
      421 ccattacggt caatccgccg tttgttccca cggagaatcc gacgggttgt tactcgctca
      481 catttaatgt tgatgaaagc tggctacagg aaggccagac gcgaattatt tttgatggcg
      541 ttggaattag cttatcgact gcacggtgca ccaatgcttc tggcgtcagg cagccatcgg
      601 aagctgtggt atggctgtgc aggtcgtaaa tcactgcata attcgtgtcg ctcaaggcgc
      661 actcccgttc tggataatgt tttttgcgcc gacatcataa cggttctggc aaatattctg
      721 aaatgagctg ttgacaatta atcatcggct cgtataatgt gtggaattgt gagcggataa
      781 caatttcaca caggaaacag tattcatgtc ccctatacta ggttattgga aaattaaggg
      841 ccttgtgcaa cccactcgac ttcttttgga atatcttgaa gaaaaatatg aagagcattt
      901 gtatgagcgc gatgaaggtg ataaatggcg aaacaaaaag tttgaattgg gtttggagtt
      961 tcccaatctt ccttattata ttgatggtga tgttaaatta acacagtcta tggccatcat
     1021 acgttatata gctgacaagc acaacatgtt gggtggttgt ccaaaagagc gtgcagagat
     1081 ttcaatgctt gaaggagcgg ttttggatat tagatacggt gtttcgagaa ttgcatatag
     1141 taaagacttt gaaactctca aagttgattt tcttagcaag ctacctgaaa tgctgaaaat
     1201 gttcgaagat cgtttatgtc ataaaacata tttaaatggt gatcatgtaa cccatcctga
     1261 cttcatgttg tatgacgctc ttgatgttgt tttatacatg gacccaatgt gcctggatgc
     1321 gttcccaaaa ttagtttgtt ttaaaaaacg tattgaagct atcccacaaa ttgataagta
     1381 cttgaaatcc agcaagtata tagcatggcc tttgcagggc tggcaagcca cgtttggtgg
     1441 tggcgaccat cctccaaaat cggatctggt tccgcgtgga tccccggaat tccaccacca
     1501 ccaccacatg tgcactctgc accccgagga ggaagctcac ctgatggcca tggccagcgg
     1561 aggacaacca acgcgtacgg ccagtccaag gccattggtc accgcctcca ttgcagcacc
     1621 acgatcgctg tttgatgtct gctgggacga tgtgctcatc ccgcaggttg ccgtctacct
     1681 gtcgctcaag gatctcttca acctgcgctg ctgctcccgt accgcacaac gctttgtgga
     1741 ggctgcgctg gaaaaaaggc aggaacttca tctctctggg aacaacacaa agaatatcga
     1801 cgtggctttt cgagtgctgg ctcgatgttg ccaacgattg gaggtgcttc atctggcctg
     1861 ctgccgatgg ctaacagatg agctgctgct gccactgctg gccaacaaca aaaaacgcct
     1921 gtgggccgtc aacctgaacg aatgcgtcaa catcacggcc ctttcgctgc agccgatcat
     1981 agtggagtgc aaggagctgc gcgtgctcaa gctgtccaag tgccagtggc tgaccacggg
     2041 agcagtggac gctttgacgc tgcaccaaag caagctggtg gagttcgaca tctcctattg
     2101 cggcgcaatt ggagagcgct gcttgattat cttcttcagg aaacttaaca agctcaccgt
     2161 cctgtcgctg gcaaatacgc ccagtgtcac cgatcaggtg ctcatccaga tcgggaacta
     2221 ttgccgcgaa ctggagcaca tcaacgtgat cgggtgtgcg gctatctccg actatggcgt
     2281 gcacgctttg acagtgcact gtttgcggct acggaccttg ctcattcgac gttgtccgcg
     2341 ggtcacagaa ctctccctgg cgccgctccg ccagcggcgt ctttacatcg ataggccaca
     2401 gccggatgtg gggctcaacg cttacaactt gaacgacttc tatcccagcg atttccttgt
     2461 ttactagcct cgactcgagc ggccgcatcg tgactgactg acgatctgcc tcgcgcgttt
     2521 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     2581 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     2641 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtataatt cttgaagacg
     2701 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     2761 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     2821 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     2881 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     2941 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     3001 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     3061 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     3121 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     3181 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     3241 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     3301 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     3361 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     3421 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga
     3481 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     3541 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     3601 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     3661 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     3721 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     3781 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     3841 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     3901 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3961 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     4021 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     4081 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     4141 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     4201 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     4261 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     4321 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4381 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     4441 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4501 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4561 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     4621 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     4681 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     4741 ttcacaccgc ataaattccg acaccatcga atggcgcaaa acctttcgcg gtatggcatg
     4801 atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgttatacga
     4861 tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag
     4921 ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat
     4981 tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac
     5041 ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga
     5101 tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa
     5161 agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct
     5221 ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct
     5281 tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg
     5341 actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc
     5401 attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa
     5461 tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca
     5521 aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca
     5581 gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat
     5641 ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac
     5701 catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc
     5761 tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac
     5821 caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     5881 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtaa
     5941 g
//
