LOCUS       sequence-267568-        8042 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  UV5 promoter drives expression of omega-erbin fusion. Pair with 
            pB1H2 UV5 Zif268-cons as a positive control (turns on HIS3/GFP)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8042)
  AUTHORS   Ichikawa DM, Corbi-Verge C, Shen MJ, Snider J, Wong V, Stagljar I, 
            Kim PM, Noyes MB
  TITLE     A Multireporter Bacterial 2-Hybrid Assay for the High-Throughput and
            Dynamic Assay of PDZ Domain-Peptide Interactions.
  JOURNAL   ACS Synth Biol. 2019 May 17;8(5):918-928. doi: 
            10.1021/acssynbio.8b00499. Epub 2019 Apr 18.
  PUBMED    30969105
REFERENCE   2  (bases 1 to 8042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.8b00499"; journalName: "ACS Synth Biol"; date: 
            "2019-05-17- 17"; volume: "8"; issue: "5"; pages: "918-928"
COMMENT     Sequence Label: pGHUC w-ERBIN (2-hybrid prey plasmid)
FEATURES             Location/Qualifiers
     source          1..8042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(414..1364)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      1412..1634
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2126..2220)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2251..3045)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2362..2381)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2972..2991
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     terminator      complement(3194..3221)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(3237..3308)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     RBS             4039..4047
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(4100..4903)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     terminator      5030..5116
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5208..5235
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     5313..5331
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(5413..5418,5419..5436,5437..5443)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     5472..5488
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5484..6146
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     RBS             6194..6202
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6208..6924
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             6208..6921
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      6939..7010
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7026..7053
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(7423..7446)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(7456..7725)
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGR"
     protein_bind    7965..7981
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7989..7995,7996..8013,8014..8019))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
ORIGIN
        1 tccagaattg ccatgatttt ttccccacgg gaggcgtcac tggctcccgt gttgtcggca
       61 gctttgattc gataagcagc atcgcctgtt tcaggctgtc tatgtgtgac tgttgagctg
      121 taacaagttg tctcaggtgt tcaatttcat gttctagttg ctttgtttta ctggtttcac
      181 ctgttctatt aggtgttaca tgctgttcat ctgttacatt gtcgatctgt tcatggtgaa
      241 cagctttgaa tgcaccaaaa actcgtaaaa gctctgatgt atctatcttt tttacaccgt
      301 tttcatctgt gcatatggac agttttccct ttgatatgta acggtgaaca gttgttctac
      361 ttttgtttgt tagtcttgat gcttcactga tagatacaag agccataaga acctcagatc
      421 cttccgtatt tagccagtat gttctctagt gtggttcgtt gtttttgcgt gagccatgag
      481 aacgaaccat tgagatcata cttactttgc atgtcactca aaaattttgc ctcaaaactg
      541 gtgagctgaa tttttgcagt taaagcatcg tgtagtgttt ttcttagtcc gttatgtagg
      601 taggaatctg atgtaatggt tgttggtatt ttgtcaccat tcatttttat ctggttgttc
      661 tcaagttcgg ttacgagatc catttgtcta tctagttcaa cttggaaaat caacgtatca
      721 gtcgggcggc ctcgcttatc aaccaccaat ttcatattgc tgtaagtgtt taaatcttta
      781 cttattggtt tcaaaaccca ttggttaagc cttttaaact catggtagtt attttcaagc
      841 attaacatga acttaaattc atcaaggcta atctctatat ttgccttgtg agttttcttt
      901 tgtgttagtt cttttaataa ccactcataa atcctcatag agtatttgtt ttcaaaagac
      961 ttaacatgtt ccagattata ttttatgaat ttttttaact ggaaaagata aggcaatatc
     1021 tcttcactaa aaactaattc taatttttcg cttgagaact tggcatagtt tgtccactgg
     1081 aaaatctcaa agcctttaac caaaggattc ctgatttcca cagttctcgt catcagctct
     1141 ctggttgctt tagctaatac accataagca ttttccctac tgatgttcat catctgagcg
     1201 tattggttat aagtgaacga taccgtccgt tctttccttg tagggttttc aatcgtgggg
     1261 ttgagtagtg ccacacagca taaaattagc ttggtttcat gctccgttaa gtcatagcga
     1321 ctaatcgcta gttcatttgc tttgaaaaca actaattcag acatacatct caattggtct
     1381 aggtgatttt aatcactata ccaattgaga tgggctagtc aatgataatt actagtcctt
     1441 ttcctttgag ttgtgggtat ctgtaaattc tgctagacct ttgctggaaa acttgtaaat
     1501 tctgctagac cctctgtaaa ttccgctaga cctttgtgtg ttttttttgt ttatattcaa
     1561 gtggttataa tttatagaat aaagaaagaa taaaaaaaga taaaaagaat agatcccagc
     1621 cctgtgtata actcactact ttagtcagtt ccgcagtatt acaaaaggat gtcgcaaacg
     1681 ctgtttgctc ctctacaaaa cagaccttaa aaccctaaag gcttaagtag caccctcgca
     1741 agctcgggca aatcgctgaa tattcctttt gtctccgacc atcaggcacc tgagtcgctg
     1801 tctttttcgt gacattcagt tcgctgcgct cacggctctg gcagtgaatg ggggtaaatg
     1861 gcactacagg cgccttttat ggattcatgc aaggaaacta cccataatac aagaaaagcc
     1921 cgtcacgggc ttctcagggc gttttatggc gggtctgcta tgtggtgcta tctgactttt
     1981 tgctgttcag cagttcctgc cctctgattt tccagtctga ccacttcgga ttatcccgtg
     2041 acaggtcatt cagactggct aatgcaccca gtaaggcagc ggtatcatca acaggcttac
     2101 ccgtcttact gtccctagtg cttggattct caccaataaa aaacgcccgg cggcaaccga
     2161 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
     2221 caagcgagct ctcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa
     2281 ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca
     2341 ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc
     2401 cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat
     2461 attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgcgcgc
     2521 cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc
     2581 ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg
     2641 gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat
     2701 gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc
     2761 gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg
     2821 aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcctgcagtt cattcagggc
     2881 accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac
     2941 ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac
     3001 ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca
     3061 tcctgtctct tgatcagatc ttgatcccct gcgccatcag atccttggcg gcaagaaagc
     3121 catccagttt actttgcagg gcttcccaac cttaccagag ggcgccccag ctggcaattc
     3181 cgacgtctat aaacgcagaa aggcccaccc gaaggtgagc cagtgtgact ctagtagaga
     3241 gcgttcaccg acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt
     3301 tttatttgat gcctggctat cattatttgt agagttcatc catgcccccc gtagagtggc
     3361 gaccttccgc acgttcatat tgttccacaa tagtataatc ttcgttatgc gaggtgatgt
     3421 ctaatttaat attcacgtta taggcccccg gcagttggac cggctttttg gctttgtagg
     3481 ttgtttttac ttcggcatca tagtgaccgc catcttttaa tttgagacgc tgcttaattt
     3541 cccctttcag tgcgccgtct tctgggtaca tacgctctga tgacgcttcc cagcccattg
     3601 tcttcttttg catcaccggg ccgtcagatg gaaaattggt gccgcgcagc tttaccttgt
     3661 aaatgaactc gccgtcttgc aggctgctgt cctgggtcac cgtcaccaca ccgccgtctt
     3721 caaagttcat tacacgttcc catttaaacc cttcaggaaa ggacaacttc aagtaatctg
     3781 gaatatcagc ggggtgtttt acgtatgctt tactaccata cataaattgt ggggataaaa
     3841 tatcccaggc gaacggcaac gggccaccct tcgtcacttt cagcttcgcc gtctgggtgc
     3901 cttcgtacgg ccgaccttcc ccctctcctt caatctcaaa ctcatggcca ttcacgctcc
     3961 cttccatatg gactttaaaa cgcatgaact ctttaatgat cgccatgttg tcctcttcgc
     4021 ccttcgacac catagctgac ctccttatta aagttaaaca aaacgccggt gctgcagctt
     4081 taaataatcg gtgtcactat tagttttgct ggccgcatct tctcaaatat gcttcccagc
     4141 ctgcttttct gtaacgttca ccctctacct tagcatccct tccctttgca aatagtcctc
     4201 ttccaacaat aataatgtca gatcctgtag agaccacatc atccacggtt ctatactgtt
     4261 gacccaatgc gtctcccttg tcatctaaac ccacaccggg tgtcataatc aaccaatcgt
     4321 aaccttcatc tcttccaccc atgtctcttt gagcaataaa gccgataaca aaatctttgt
     4381 cgctcttcgc aatgtcaaca gtacccttag tatattctcc agtagatagg gagcccttgc
     4441 atgacaattc tgctaacatc aaaaggcctc taggttcctt tgttacttct tctgccgcct
     4501 gcttcaaacc gctaacaata cctgggccca ccacaccgtg tgcattcgta atgtctgccc
     4561 attctgctat tctgtataca cccgcagagt actgcaattt gactgtatta ccaatgtcag
     4621 caaattttct gtcttcgaag agtaaaaaat tgtacttggc ggataatgcc tttagcggct
     4681 taactgtgcc ctccatggaa aaatcagtca agatatccac atgtgttttt agtaaacaaa
     4741 ttttgggacc taatgcttca actaactcca gtaattcctt ggtggtacga acatccaatg
     4801 aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa tagcttggca gcaacaggac
     4861 taggatgagt agcagcacgt tccttatatg tagctttcga catagctgtt tcctgtgtgg
     4921 ctaccagtag cgtattactt gaggaacata agccgaaagg ccataggatg taaaaccggt
     4981 gaccgatatg cctaacgccc acgcgcgtgg gcgttggcgc gccaggcatc aaataaaacg
     5041 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     5101 cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg
     5161 gtggcgggca ggacgcccgc cataaactgc caggcatcaa attaagcaga aggccatcct
     5221 gacggatggc ctttttgcgt ttctacaaac tcttttgttt atttttctaa atacattcaa
     5281 atatgtatcc gctcatgaca ttaacctgta aaaataggcg tatcacgagg ccctttcgtc
     5341 ttcaaacgcg tgtacaaaac cggcggccgc aaacgcccac gcgcgtgggc gttaggcata
     5401 tcggtcgaat tctttacact ttatgcttcc ggctcgtatg ttgtgtcgac cgagcggata
     5461 acaatttcac acaggaaaca gctatgacag agcagaaagc cctagtaaag cgtattacaa
     5521 atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact
     5581 cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga
     5641 ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt
     5701 ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca
     5761 ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctaggg gccgtgcgtg
     5821 gagtaaaaag gtttggatca ggatttgcgc ctttggatga ggcactttcc agagcggtgg
     5881 tagatctttc gaacaggccg tacgcagttg tcgaacttgg tttgcaaagg gagaaagtag
     5941 gagatctctc ttgcgagatg atcccgcatt ttcttgaaag ctttgcagag gctagcagaa
     6001 ttaccctcca cgttgattgt ctgcgaggca agaatgatca tcaccgtagt gagagtgcgt
     6061 tcaaggctct tgcggttgcc ataagagaag ccacctcgcc caatggtacc aacgatgttc
     6121 cctccaccaa aggtgttctt atgtagtgac accgattatt taaagctgca gggatccttt
     6181 tgtttaactt taataaggag gtcagctatg tctaaaggtg aagaattatt cactggtgtt
     6241 gtcccaattt tggttgaatt agatggtgat gttaatggtc acaaattttc tgtctccggt
     6301 gaaggtgaag gtgatgctac ttacggtaaa ttgaccttaa aatttatttg tactactggt
     6361 aaattgccag ttccatggcc aaccttagtc actactttaa cttatggtgt tcaatgtttt
     6421 tcaagatacc cagatcatat gaaacaacat gactttttca agtctgccat gccagaaggt
     6481 tatgttcaag aaagaactat ttttttcaaa gatgacggta actacaagac cagagctgaa
     6541 gtcaagtttg aaggtgatac cttagttaat agaatcgaat taaaaggtat tgattttaaa
     6601 gaagatggta acattttagg tcacaaattg gaatacaact ataactctca caatgtttac
     6661 atcatggctg acaaacaaaa gaatggtatc aaagttaact tcaaaattag acacaacatt
     6721 gaagatggtt ctgttcaatt agctgaccat tatcaacaaa atactccaat tggtgatggt
     6781 ccagtcttgt taccagacaa ccattactta tccactcaat ctgccttatc caaagatcca
     6841 aacgaaaaga gagaccacat ggtcttgtta gaatttgtta ctgctgctgg tattacccat
     6901 ggtatggatg aattgtacaa ataatagtga ccaggcatca aataaaacga aaggctcagt
     6961 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     7021 acactggctc accttcgggt gggcctttct gcgtttatac tcgagctatt agctgctcac
     7081 ttcgcgcaca ataatcagtt ccacggtgtt ctgaaaggtt ttcagcaggc tcaccgcctg
     7141 gccatgttca atgttaataa agctatagcc gttcgcctga ataattttat cgcccggctg
     7201 cagcagtttg ctcgccgggc cttccggctg cacgcgggtc acaaaaatgc catcatcatc
     7261 cgggcgaaac gggttgccac gaccgcccac gccgccgcta atgctaaagc ccagttccgg
     7321 atctttttcc acgcgcacgc gaatttcctg tttcgccagt tcatggccca tatggctgcc
     7381 cgatcgtcta gaatgcggag gtgtcttgga gcctgtccgg aacttgtcgt cgtcatcctt
     7441 gtagtccgcc gccgcacgac cttcagcaat agcggtaacg gcttgtaatt cagcggcttc
     7501 ctgctcttgc tgttcctggc gttcgcgaac gtcgaggatc tggttgttga tcagaccttc
     7561 ttcgatttcg cgcagcgcga ttacagtggt tttatcgttt tcttccggta cgagcgggtc
     7621 ctttccgcct acctgcatct gacgagcgcg acgcgcggcg accagtacca ggtcaaaacg
     7681 gttaccaatt ttctctacag cgtcctgaac agttacgcgt gccatgggta tatctccttc
     7741 ttggagccga gacttacaat tgtgtttttt caaaattctt gcaggtttcg atggcttctt
     7801 gtaataagct gcgttgctcc tctggagaaa agatttggtt aatttggttg aggacgtttg
     7861 gtacggtatt ttgtgatgat tgctgttgca aagggttgtt gtccgcactt gaattgggag
     7921 gttgcatttg ctgctgttga ggcatagctg tttcctgtgt gaaattgtta tccgctcaca
     7981 agtcgacaca ttatacgagc cggaagcata aagtgtaaag cccgcccggg agcgctattt
     8041 ct
//
