LOCUS       sequence-267571-        6962 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression of D. melanogaster (Dm) cryptochrome with 
            C-terminal V5 and His tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Zhu H, Yuan Q, Briscoe AD, Froy O, Casselman A, Reppert SM
  TITLE     The two CRYs of the butterfly.
  JOURNAL   Curr Biol. 2005 Dec 6;15(23):R953-4. doi: 10.1016/j.cub.2005.11.030.
  PUBMED    16332522
REFERENCE   2  (bases 1 to 6962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2005.11"; journalName: "Curr Biol"; date: "2005-12-6-
            6"; volume: "15"; issue: "23"; pages: "R953-4"
COMMENT     Sequence Label: pAC5.1-dCry-V5His
FEATURES             Location/Qualifiers
     source          1..6962
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3027..3068
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3078..3095
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3118..3135)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3168..3302
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3192..3211)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3246..3265
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(3903..4491)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3983..4002)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4662..5453,5454..5522))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5285..5304
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5523..5627)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5695..5713
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(5777..6962,1..1324)
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
ORIGIN
        1 ccatgtgtgt gcagccaacg atagcaacaa aagcaacaac acaggtggct ttccctcttt
       61 cactttttgt ttgcaagccg cgtgcgagca agacggcacg accggcaaac gcaattacgc
      121 tgacaaagag cagacgaagt tttggcgaaa aacatcaagg cgcctgatac gaatgcattt
      181 gcaataacaa ttgcgatatt taatattgtt tatgaagctg tttgacttca aaacacacaa
      241 aaaaaaaaat aaaacaaatt atttgaaaga gaattaggaa tcggacgctt atcgttaggg
      301 taacaacaag aaatgcttac tgagtcacag cctctggaaa actgccgcaa gccagagaga
      361 gagagaaaaa gagggagagc agcttagacc gcatgtgctt gtgtgtgagg cgtctctctc
      421 ttcgtctctg ttgcgcaaac gcatagactg cactgaaaaa atcgattacc tattttttat
      481 gaatgaatat ttgcactatt actattcaaa actattaaga tagcaatcac attcaatagc
      541 caaatactat accacctgag cgatgcaacg aaatgatcaa tttgagcaaa aatgctgcat
      601 atttaggacg gcatcattat agaaatgctt cttgctgtgt acttttctct cgtctggcag
      661 ctgtttcgcc gttattgtta aaaccggctt aagttaggtg tgttttctac gactagtgaa
      721 tgccctacta gaagatgtgt gttgcacaaa atgtccctgg aataaccaat ttgaagtgca
      781 gatagcagta aacgtaagct aatatgaata ttatttaact gtaatgtttt aatatcgctg
      841 gacattacta ataaacccac tataaacaca tgtacatatg tatgttttgg catacaatga
      901 gtagttgggg aaaaaatgtg taaaagcacc gtgaccatca cagcataaag ataaccagct
      961 gaagtatcga atatgagtaa cccccaaatt gaatcacatg ccgcaactga taggacccat
     1021 ggaagtacac tcttcatggc gatatacaag acacacacaa gcacgaacac ccagttgcgg
     1081 aggaaattct ccgtaaatga aaacccaatc ggcgaacaat tcatacccat atatggtaaa
     1141 agttttgaac gcgacttgag agcggagagc attgcggctg ataaggtttt agcgctaagc
     1201 gggctttata aaacgggctg cgggaccagt tttcatatca ctaccgtttg agttcttgtg
     1261 ctgtgtggat actcctcccg acacaaagcc gctccatcag ccagcagtcg tctaatccag
     1321 agaccccgga tcggggtacc tactagtcca gtgtggtgga attcaccatg gccacgcgag
     1381 gggcgaatgt gatttggttt cgccatggat tgcgcctcca tgataatccc gctctattgg
     1441 ccgccctcgc cgataaggat cagggtatag ccctaattcc cgttttcata ttcgatggag
     1501 agagtgcagg taccaagaat gtgggttaca atcggatgcg tttcctcctg gactcgttgc
     1561 aggacatcga tgatcagcta caggcggcaa ctgatggacg tggacgcctc ctggtcttcg
     1621 agggcgaacc ggcttatatc ttccgccggc tacatgagca agtgcgtctg cacaggattt
     1681 gcatagagca ggactgcgag ccaatttgga atgagcgcga tgaaagcatc cgttctctat
     1741 gtcgggagct gaatatcgac tttgtcgaga aggtatcaca cacgctttgg gatccgcaat
     1801 tggtgattga gaccaatggt ggcattccac cgctgaccta ccaaatgttc ctgcacacgg
     1861 tgcaaattat tgggcttcca ccgcgtccca ccgccgatgc tcgactagaa gacgccacct
     1921 ttgtcgagct ggaccccgag ttctgccgaa gtcttaagtt gttcgagcag ctgcccacgc
     1981 cggagcactt caatgtgtat ggagacaaca tgggcttcct ggccaagatt aactggcgcg
     2041 gcggagaaac acaggcctta cttctgttgg atgagcgtct taaagtggag cagcatgcgt
     2101 ttgagcgtgg attttatctg cccaaccagg cactgcccaa tatccacgac tcgccaaaat
     2161 cgatgagcgc ccatctgcgc tttggttgcc tttcggtacg tcgcttctac tggagcgtcc
     2221 acgatctctt caagaatgtc cagttgcgcg cctgtgtgcg gggcgttcag atgactggcg
     2281 gcgcgcacat cacgggacag ttgatctggc gagagtactt ctacaccatg tcggtgaaca
     2341 atccaaacta cgatcgcatg gagggcaatg acatctgcct gagcatcccg tgggctaagc
     2401 cgaacgaaaa tctcctgcag agctggcgtt taggccaaac gggattcccg ctcatcgacg
     2461 gcgccatgcg acaactcctg gccgagggat ggctccacca tacgctgcgc aacaccgtgg
     2521 ccacctttct cacgcgcggc ggtttgtggc agagctggga gcatggactg cagcactttc
     2581 tgaagtatct gctggatgcg gattggtcgg tctgcgctgg caactggatg tgggtatcca
     2641 gctcggcgtt tgaaaggctg ctggactcct ccctggtcac ctgcccggtg gcattggcca
     2701 agcgacttga tccggatggc acctacatca agcagtacgt cccggagttg atgaatgtgc
     2761 ccaaggaatt tgttcacgag ccctggcgaa tgtctgccga gcagcaggag cagtacgagt
     2821 gcctgatcgg agtccattat ccggagcgga tcattgattt gtccatggcc gtaaagcgaa
     2881 acatgctggc catgaagtct ctccgaaatt cgctgatcac cccgccgccg cattgccgac
     2941 catccaacga ggaggaagtg cgtcagttct tctggctggc cgacgtggtg gtttggcggc
     3001 cgctcgagtc tagagggccc ttcgaaggta agcctatccc taaccctctc ctcggtctcg
     3061 attctacgcg taccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct
     3121 cgactgtgcc ttctaaggcc tgagctcgct gatcagcctc gatcgaggat ccagacatga
     3181 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     3241 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     3301 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     3361 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcag tcgaccgatg
     3421 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
     3481 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
     3541 tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt
     3601 gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa
     3661 cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc
     3721 ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc
     3781 ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat
     3841 cagggacagc ttcaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     3901 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     3961 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     4021 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     4081 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     4141 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     4201 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4261 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4321 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     4381 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     4441 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4501 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4561 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     4621 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     4681 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     4741 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     4801 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     4861 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     4921 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca
     4981 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     5041 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     5101 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     5161 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     5221 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca
     5281 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     5341 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     5401 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     5461 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     5521 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     5581 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     5641 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     5701 cgtatcacga ggccctttcg tcttcaagaa ttaattctat attctaaaaa cacaaatgat
     5761 acttctaaaa aaaaatcatg aatggcatca actctgaatc aaatctttgc agatgcacct
     5821 acttctcatt tccactgtca catcattttt ccagatctcg ctgcctgtta tgtggcccac
     5881 aaaccaagac acgttttatg gccattaaag ctggctgatc gtcgccaaac accaaataca
     5941 taatgaatat gtacacattc gagaaagaag cgatcaaaga agcgtcttcg ggcggagtag
     6001 gagaatgcgg aggagaagga gaacgagctg atctagtatc tctccacaat ccaatgccaa
     6061 ctgaccaact ggccatattc ggagcaattt gaagccaatt tccatcgcct ggcgatcgct
     6121 ccattcttgg ctatatgttt ttcaccgtta cccggggcca ttttcaaaga ctcgtcggca
     6181 agataagatt gtgtcactcg ctgtctctct tcatttgtcg aagaatgctg aggaatttcg
     6241 cgatgacgtc ggcgagtatt ttgaagaatg agaataattt gtatttatac gaaaatcagt
     6301 tagtggaatt ttctacaaaa acatgttatc tatagataat tttgttgcaa aatatgttga
     6361 ctatgacaaa gattgtatgt atataccttt aatgtattct cattttctta tgtatttata
     6421 atggcaatga tgatactgat gatattttaa gatgatgcca gaccaaaagg cttgaatttc
     6481 tgcgtctttt gccgaacgca gtgcatgtgc aattgttgtt ttttggaata ttcaattttc
     6541 ggactgtccg ctttgatttc agtttcttgg cttattcaaa aagcaaagta aagccaaaaa
     6601 agcgagatgg caataccaaa tgcggcaaaa cggtagtgga aggaaagggg tgcggggcag
     6661 cggaaggaag ggtggggcgg ggcgtggcgg ggtctgtggc tgggcgcgac gtcaccgacg
     6721 ttggagccac tcctttgacc atgtgtgcgt gtgtgtatta ttcgtgtctc gccactcgcc
     6781 ggttgttttt ttctttttat gctgcgctct ctctagcgcc atctcgctta cgcatgctca
     6841 acgcaccgca tgttgccgtt tccttttatg cgtcattttg gctcgaaata ggcaattatt
     6901 taaacaaaga ttagtcaacg aaaacgctaa aataaataag tctacaatat ggttacttat
     6961 tg
//
