LOCUS       sequence-267572-        8409 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression of Beta-galactosidase with C-terminal V5 and His 
            tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8409)
  AUTHORS   Zhu H, Yuan Q, Briscoe AD, Froy O, Casselman A, Reppert SM
  TITLE     The two CRYs of the butterfly.
  JOURNAL   Curr Biol. 2005 Dec 6;15(23):R953-4. doi: 10.1016/j.cub.2005.11.030.
  PUBMED    16332522
REFERENCE   2  (bases 1 to 8409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2005.11"; journalName: "Curr Biol"; date: "2005-12-6-
            6"; volume: "15"; issue: "23"; pages: "R953-4"
COMMENT     Sequence Label: pAC5.1-bgal-V5His
FEATURES             Location/Qualifiers
     source          1..8409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1399..4443
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGSLSQMWIGDKNQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(1401..1423)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             4471..4512
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4522..4539
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4562..4579)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4612..4746
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4636..4655)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4690..4709
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(5347..5935)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5427..5446)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6106..6897,6898..6966))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6729..6748
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6967..7071)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7139..7157
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(7221..8409,1..1321)
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
ORIGIN
        1 tgtgtgtgca gccaacgata gcaacaaaag caacaacaca ggtggctttc cctctttcac
       61 tttttgtttg caagccgcgt gcgagcaaga cggcacgacc ggcaaacgca attacgctga
      121 caaagagcag acgaagtttt ggcgaaaaac atcaaggcgc ctgatacgaa tgcatttgca
      181 ataacaattg cgatatttaa tattgtttat gaagctgttt gacttcaaaa cacacaaaaa
      241 aaaaaataaa acaaattatt tgaaagagaa ttaggaatcg gacgcttatc gttagggtaa
      301 caacaagaaa tgcttactga gtcacagcct ctggaaaact gccgcaagcc agagagagag
      361 agaaaaagag ggagagcagc ttagaccgca tgtgcttgtg tgtgaggcgt ctctctcttc
      421 gtctctgttg cgcaaacgca tagactgcac tgaaaaaatc gattacctat tttttatgaa
      481 tgaatatttg cactattact attcaaaact attaagatag caatcacatt caatagccaa
      541 atactatacc acctgagcga tgcaacgaaa tgatcaattt gagcaaaaat gctgcatatt
      601 taggacggca tcattataga aatgcttctt gctgtgtact tttctctcgt ctggcagctg
      661 tttcgccgtt attgttaaaa ccggcttaag ttaggtgtgt tttctacgac tagtgaatgc
      721 cctactagaa gatgtgtgtt gcacaaaatg tccctggaat aaccaatttg aagtgcagat
      781 agcagtaaac gtaagctaat atgaatatta tttaactgta atgttttaat atcgctggac
      841 attactaata aacccactat aaacacatgt acatatgtat gttttggcat acaatgagta
      901 gttggggaaa aaatgtgtaa aagcaccgtg accatcacag cataaagata accagctgaa
      961 gtatcgaata tgagtaaccc ccaaattgaa tcacatgccg caactgatag gacccatgga
     1021 agtacactct tcatggcgat atacaagaca cacacaagca cgaacaccca gttgcggagg
     1081 aaattctccg taaatgaaaa cccaatcggc gaacaattca tacccatata tggtaaaagt
     1141 tttgaacgcg acttgagagc ggagagcatt gcggctgata aggttttagc gctaagcggg
     1201 ctttataaaa cgggctgcgg gaccagtttt catatcacta ccgtttgagt tcttgtgctg
     1261 tgtggatact cctcccgaca caaagccgct ccatcagcca gcagtcgtct aatccagaga
     1321 ccccggatcg gggtaccgag ctcggatcca ctagtccagt gtggtggaat tctgcagatc
     1381 gaaacgatga tagatcccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     1441 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     1501 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg
     1561 tttccggcac cagaagcggt gccggaaagc tggctggagt gcgatcttcc tgaggccgat
     1621 actgtcgtcg tcccctcaaa ctggcagatg cacggttacg atgcgcccat ctacaccaac
     1681 gtgacctatc ccattacggt caatccgccg tttgttccca cggagaatcc gacgggttgt
     1741 tactcgctca catttaatgt tgatgaaagc tggctacagg aaggccagac gcgaattatt
     1801 tttgatggcg ttaactcggc gtttcatctg tggtgcaacg ggcgctgggt cggttacggc
     1861 caggacagtc gtttgccgtc tgaatttgac ctgagcgcat ttttacgcgc cggagaaaac
     1921 cgcctcgcgg tgatggtgct gcgctggagt gacggcagtt atctggaaga tcaggatatg
     1981 tggcggatga gcggcatttt ccgtgacgtc tcgttgctgc ataaaccgac tacacaaatc
     2041 agcgatttcc atgttgccac tcgctttaat gatgatttca gccgcgctgt actggaggct
     2101 gaagttcaga tgtgcggcga gttgcgtgac tacctacggg taacagtttc tttatggcag
     2161 ggtgaaacgc aggtcgccag cggcaccgcg cctttcggcg gtgaaattat cgatgagcgt
     2221 ggtggttatg ccgatcgcgt cacactacgt ctgaacgtcg aaaacccgaa actgtggagc
     2281 gccgaaatcc cgaatctcta tcgtgcggtg gttgaactgc acaccgccga cggcacgctg
     2341 attgaagcag aagcctgcga tgtcggtttc cgcgaggtgc ggattgaaaa tggtctgctg
     2401 ctgctgaacg gcaagccgtt gctgattcga ggcgttaacc gtcacgagca tcatcctctg
     2461 catggtcagg tcatggatga gcagacgatg gtgcaggata tcctgctgat gaagcagaac
     2521 aactttaacg ccgtgcgctg ttcgcattat ccgaaccatc cgctgtggta cacgctgtgc
     2581 gaccgctacg gcctgtatgt ggtggatgaa gccaatattg aaacccacgg catggtgcca
     2641 atgaatcgtc tgaccgatga tccgcgctgg ctaccggcga tgagcgaacg cgtaacgcga
     2701 atggtgcagc gcgatcgtaa tcacccgagt gtgatcatct ggtcgctggg gaatgaatca
     2761 ggccacggcg ctaatcacga cgcgctgtat cgctggatca aatctgtcga tccttcccgc
     2821 ccggtgcagt atgaaggcgg cggagccgac accacggcca ccgatattat ttgcccgatg
     2881 tacgcgcgcg tggatgaaga ccagcccttc ccggctgtgc cgaaatggtc catcaaaaaa
     2941 tggctttcgc tacctggaga gacgcgcccg ctgatccttt gcgaatacgc ccacgcgatg
     3001 ggtaacagtc ttggcggttt cgctaaatac tggcaggcgt ttcgtcagta tccccgttta
     3061 cagggcggct tcgtctggga ctgggtggat cagtcgctga ttaaatatga tgaaaacggc
     3121 aacccgtggt cggcttacgg cggtgatttt ggcgatacgc cgaacgatcg ccagttctgt
     3181 atgaacggtc tggtctttgc cgaccgcacg ccgcatccag cgctgacgga agcaaaacac
     3241 cagcagcagt ttttccagtt ccgtttatcc gggcaaacca tcgaagtgac cagcgaatac
     3301 ctgttccgtc atagcgataa cgagctcctg cactggatgg tggcgctgga tggtaagccg
     3361 ctggcaagcg gtgaagtgcc tctggatgtc gctccacaag gtaaacagtt gattgaactg
     3421 cctgaactac cgcagccgga gagcgccggg caactctggc tcacagtacg cgtagtgcaa
     3481 ccgaacgcga ccgcatggtc agaagccggg cacatcagcg cctggcagca gtggcgtctg
     3541 gcggaaaacc tcagtgtgac gctccccgcc gcgtcccacg ccatcccgca tctgaccacc
     3601 agcgaaatgg atttttgcat cgagctgggt aataagcgtt ggcaatttaa ccgccagtca
     3661 ggctctcttt cacagatgtg gattggcgat aaaaaccaac tgctgacgcc gctgcgcgat
     3721 cagttcaccc gtgcaccgct ggataacgac attggcgtaa gtgaagcgac ccgcattgac
     3781 cctaacgcct gggtcgaacg ctggaaggcg gcgggccatt accaggccga agcagcgttg
     3841 ttgcagtgca cggcagatac acttgctgat gcggtgctga ttacgaccgc tcacgcgtgg
     3901 cagcatcagg ggaaaacctt atttatcagc cggaaaacct accggattga tggtagtggt
     3961 caaatggcga ttaccgttga tgttgaagtg gcgagcgata caccgcatcc ggcgcggatt
     4021 ggcctgaact gccagctggc gcaggtagca gagcgggtaa actggctcgg attagggccg
     4081 caagaaaact atcccgaccg ccttactgcc gcctgttttg accgctggga tctgccattg
     4141 tcagacatgt ataccccgta cgtcttcccg agcgaaaacg gtctgcgctg cgggacgcgc
     4201 gaattgaatt atggcccaca ccagtggcgc ggcgacttcc agttcaacat cagccgctac
     4261 agtcaacagc aactgatgga aaccagccat cgccatctgc tgcacgcgga agaaggcaca
     4321 tggctgaata tcgacggttt ccatatgggg attggtggcg acgactcctg gagcccgtca
     4381 gtatcggcgg aattccagct gagcgccggt cgctaccatt accagttggt ctggtgtcaa
     4441 aaagcggccg ctcgaggtca cccattcgaa ggtaagccta tccctaaccc tctcctcggt
     4501 ctcgattcta cgcgtaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca
     4561 gcctcgactg tgccttctaa ggcctgagct cgctgatcag cctcgatcga ggatccagac
     4621 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     4681 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     4741 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     4801 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcagtcgacc
     4861 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat
     4921 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc
     4981 gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc
     5041 gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac
     5101 caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta
     5161 cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc
     5221 ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga
     5281 ccatcaggga cagcttcaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5341 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5401 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5461 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5521 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5581 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     5641 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     5701 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     5761 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     5821 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     5881 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     5941 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     6001 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     6061 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     6121 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     6181 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     6241 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     6301 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     6361 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     6421 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     6481 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     6541 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     6601 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     6661 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     6721 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     6781 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     6841 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     6901 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     6961 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     7021 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     7081 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     7141 taggcgtatc acgaggccct ttcgtcttca agaattaatt ctatattcta aaaacacaaa
     7201 tgatacttct aaaaaaaaat catgaatggc atcaactctg aatcaaatct ttgcagatgc
     7261 acctacttct catttccact gtcacatcat ttttccagat ctcgctgcct gttatgtggc
     7321 ccacaaacca agacacgttt tatggccatt aaagctggct gatcgtcgcc aaacaccaaa
     7381 tacataatga atatgtacac attcgagaaa gaagcgatca aagaagcgtc ttcgggcgga
     7441 gtaggagaat gcggaggaga aggagaacga gctgatctag tatctctcca caatccaatg
     7501 ccaactgacc aactggccat attcggagca atttgaagcc aatttccatc gcctggcgat
     7561 cgctccattc ttggctatat gtttttcacc gttacccggg gccattttca aagactcgtc
     7621 ggcaagataa gattgtgtca ctcgctgtct ctcttcattt gtcgaagaat gctgaggaat
     7681 ttcgcgatga cgtcggcgag tattttgaag aatgagaata atttgtattt atacgaaaat
     7741 cagttagtgg aattttctac aaaaacatgt tatctataga taattttgtt gcaaaatatg
     7801 ttgactatga caaagattgt atgtatatac ctttaatgta ttctcatttt cttatgtatt
     7861 tataatggca atgatgatac tgatgatatt ttaagatgat gccagaccaa aaggcttgaa
     7921 tttctgcgtc ttttgccgaa cgcagtgcat gtgcaattgt tgttttttgg aatattcaat
     7981 tttcggactg tccgctttga tttcagtttc ttggcttatt caaaaagcaa agtaaagcca
     8041 aaaaagcgag atggcaatac caaatgcggc aaaacggtag tggaaggaaa ggggtgcggg
     8101 gcagcggaag gaagggtggg gcggggcgtg gcggggtctg tggctgggcg cgacgtcacc
     8161 gacgttggag ccactccttt gaccatgtgt gcgtgtgtgt attattcgtg tctcgccact
     8221 cgccggttgt ttttttcttt ttatgctgcg ctctctctag cgccatctcg cttacgcatg
     8281 ctcaacgcac cgcatgttgc cgtttccttt tatgcgtcat tttggctcga aataggcaat
     8341 tatttaaaca aagattagtc aacgaaaacg ctaaaataaa taagtctaca atatggttac
     8401 ttattgcca
//
