LOCUS       sequence-267587-        5934 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Ricci MS, Kim SH, Ogi K, Plastaras JP, Ling J, Wang W, Jin Z, Liu 
            YY, Dicker DT, Chiao PJ, Flaherty KT, Smith CD, El-Deiry WS
  TITLE     Reduction of TRAIL-induced Mcl-1 and cIAP2 by c-Myc or sorafenib 
            sensitizes resistant human cancer cells to TRAIL-induced death.
  JOURNAL   Cancer Cell. 2007 Jul . 12(1):66-80.
  PUBMED    17613437
REFERENCE   2  (bases 1 to 5934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell. 2007 Jul . 12(1):66-80."
COMMENT     Sequence Label: pGL2-Mcl-1
FEATURES             Location/Qualifiers
     source          1..5934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             430..2082
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(496..514)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          2323..2388
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2518..2538
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2963..3097
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3239..3256)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3410..3998)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3490..3509)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4169..4960,4961..5029))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4792..4811
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5030..5134)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5161..5616
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5248..5267)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5458..5479
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5806..5927
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 gtaactgagc taacataacc cgggaggtac cgagctctta cgcgtgctag ctcgagccca
       61 ggagctctgg gaggacccca ggcgctcgga gccgccgtta cgtaaccggc actcagagcc
      121 tccgaagacc ggaaggcccc gctcaggccc cggccccggc cccggccccg ccccggcccg
      181 gccgggcagc tggtaggtgc cgtgcgcaac cctccggaag ctgccgcccc tttccccttt
      241 tatgggaata ctttttttaa aaaaaaagag ttcgctggcg ccaccccgta ggactggccg
      301 ccctaaaacc gtgataaagg agctgctcgc cacttctcac ttccgcttcc ttccagtaag
      361 gagtcggggt cttccccagt tttctcagcc aggcggcggc aagcttggca ttccggtact
      421 gttggtaaaa tggaagacgc caaaaacata aagaaaggcc cggcgccatt ctatcctcta
      481 gaggatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct
      541 ggaacaattg cttttacaga tgcacatatc gaggtgaaca tcacgtacgc ggaatacttc
      601 gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga
      661 atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt
      721 atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt
      781 atgaacattt cgcagcctac cgtagtgttt gtttccaaaa aggggttgca aaaaattttg
      841 aacgtgcaaa aaaaattacc aataatccag aaaattatta tcatggattc taaaacggat
      901 taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc cggttttaat
      961 gaatacgatt ttgtaccaga gtcctttgat cgtgacaaaa caattgcact gataatgaat
     1021 tcctctggat ctactgggtt acctaagggt gtggcccttc cgcatagaac tgcctgcgtc
     1081 agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga tactgcgatt
     1141 ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg atatttgata
     1201 tgtggatttc gagtcgtctt aatgtataga tttgaagaag agctgttttt acgatccctt
     1261 caggattaca aaattcaaag tgcgttgcta gtaccaaccc tattttcatt cttcgccaaa
     1321 agcactctga ttgacaaata cgatttatct aatttacacg aaattgcttc tgggggcgca
     1381 cctctttcga aagaagtcgg ggaagcggtt gcaaaacgct tccatcttcc agggatacga
     1441 caaggatatg ggctcactga gactacatca gctattctga ttacacccga gggggatgat
     1501 aaaccgggcg cggtcggtaa agttgttcca ttttttgaag cgaaggttgt ggatctggat
     1561 accgggaaaa cgctgggcgt taatcagaga ggcgaattat gtgtcagagg acctatgatt
     1621 atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa ggatggatgg
     1681 ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat agttgaccgc
     1741 ttgaagtctt taattaaata caaaggatat caggtggccc ccgctgaatt ggaatcgata
     1801 ttgttacaac accccaacat cttcgacgcg ggcgtggcag gtcttcccga cgatgacgcc
     1861 ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag
     1921 atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg
     1981 tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag
     2041 atcctcataa aggccaagaa gggcggaaag tccaaattgt aaaatgtaac tgtattcagc
     2101 gatgacgaaa ttcttagcta ttgtaatact gcgatgagtg gcagggcggg gcgtaatttt
     2161 tttaaggcag ttattggtgc ccttaaacgc ctggttgcta cgcctgaata agtgataata
     2221 agcggatgaa tggcagaaat tcgccggatc tttgtgaagg aaccttactt ctgtggtgtg
     2281 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     2341 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     2401 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
     2461 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
     2521 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
     2581 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
     2641 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
     2701 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
     2761 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
     2821 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
     2881 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     2941 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     3001 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     3061 gtccaaactc atcaatgtat cttatcatgt ctggatccgt cgaccgatgc ccttgagagc
     3121 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
     3181 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct tccgcttcct
     3241 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     3301 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     3361 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     3421 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     3481 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3541 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3601 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     3661 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     3721 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     3781 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     3841 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     3901 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     3961 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     4021 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     4081 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     4141 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     4201 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     4261 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     4321 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     4381 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     4441 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     4501 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     4561 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     4621 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     4681 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     4741 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     4801 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     4861 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     4921 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     4981 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     5041 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     5101 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     5161 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     5221 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     5281 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     5341 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     5401 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     5461 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     5521 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     5581 acgcgaattt taacaaaata ttaacgctta caatttgcca ttcgccattc aggctgcgca
     5641 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagccc aagctaccat
     5701 gataagtaag taatattaag gtacgtggag gttttacttg ctttaaaaaa cctcccacac
     5761 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     5821 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     5881 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatgg tact
//
