LOCUS       sequence-267588-        9848 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Components for genome editing in mammalian cells with 
            pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA and pBS-U6-crRNA-targeted. This 
            plasmid was optimized for iPS cells and coexpresses mCherry 
            protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9848)
  AUTHORS   Morisaka H, Yoshimi K, Okuzaki Y, Gee P, Kunihiro Y, Sonpho E, Xu H,
            Sasakawa N, Naito Y, Nakada S, Yamamoto T, Sano S, Hotta A, Takeda 
            J, Mashimo T
  TITLE     CRISPR-Cas3 induces broad and unidirectional genome editing in human
            cells.
  JOURNAL   Nat Commun. 2019 Dec 6;10(1):5302. doi: 10.1038/s41467-019-13226-x.
  PUBMED    31811138
REFERENCE   2  (bases 1 to 9848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-6"; volume: "10(1):5302. doi"; pages: " 
            10.1038/s41467-019-13226-x"
COMMENT     Sequence Label: pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA
FEATURES             Location/Qualifiers
     source          1..9848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   46..108
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        578..1756
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          809..1747
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1704..1724
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1779..1803
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1850..1859
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1862..1882
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3017..3037
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3047..3103
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3107..3127
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3845..3865
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3875..3928
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3932..3952
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5468..5488
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(5515..5539)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(5581..6155,6156..6158,6159..6167)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5748..5765)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5975..5994
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6168..6878
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6313..6331)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6581..6600
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(6992..7011)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7057..7112
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7111..7130)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     repeat_region   7815..7849
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7942..7959)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8113..8701)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8193..8212)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8872..9663,9664..9732))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9495..9514
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9733..9837)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaaaagtgcc acctgacgtc atctgttaac attatacgcg tttaacccta gaaagataat
       61 catattgtga cgtacgttaa agataatcat gcgtaaaatt gacgcatgtg ttttatcggt
      121 ctgtatatcg aggtttattt attaatttga atagatatta agttttatta tatttacact
      181 tacatactaa taataaattc aacaaacaat ttatttatgt ttatttattt attaaaaaaa
      241 aacaaaaact caaaatttct tctataaagt aacaaaactt ttaaacattc tctcttttac
      301 aaaaataaac ttattttgta ctttaaaaac agtcatgttg tattataaaa taagtaatta
      361 gcttaaccta tacataatag aaacaaatta tacttattag tcagtcagaa acaactttgg
      421 cacatatcaa tattatgctc tgctagcgtt taaacccggg cgatcgctct agactagtgc
      481 ggccgctgca gaagctttgc aaagatggat aaagttttaa acagagagga atctttgcag
      541 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      601 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      661 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      721 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      781 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      841 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      901 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      961 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
     1021 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
     1081 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
     1141 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
     1201 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
     1261 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
     1321 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
     1381 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
     1441 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1501 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1561 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1621 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1681 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1741 atttcaggtg tcgtgaggaa ttagcggtgg cggccatcac aagtttgtac aaaaaagcag
     1801 gctggcgccg gaaccaattc agtcgactgg atccggtacc gaattcgccg ccaccatggc
     1861 cccaaagaag aagagaaagg tgagatctga atccaatttc atcaacatcc acgtgctgat
     1921 ctcccactct ccaagctgcc tgaatagaga cgatatgaac atgcagaagg acgccatctt
     1981 tggcggcaag cggagagtga ggatctctag ccagtccctg aagcgggcca tgagaaagtc
     2041 tggctactat gcccagaata tcggcgagtc ctctctgagg accatccacc tggcacagct
     2101 gagggacgtg ctgagacaga agctgggcga gcggtttgat cagaagatca tcgacaagac
     2161 actggccctg ctgagcggca agtccgtgga tgaggccgag aagatcagcg ccgacgcagt
     2221 gaccccatgg gtggtgggag agatcgcatg gttctgtgag caggtggcca aggccgaggc
     2281 cgataatctg gacgataaga agctgctgaa ggtgctgaag gaggatatcg ccgccatcag
     2341 agtgaacctg cagcagggag tggacatcgc cctgagcggc aggatggcca catccggcat
     2401 gatgaccgag ctgggcaagg tggacggagc aatgtccatc gcacacgcca tcaccacaca
     2461 ccaggtggac tctgatatcg actggttcac agccgtggac gatctgcagg agcagggaag
     2521 cgcccacctg ggaacccagg agttcagctc cggcgtgttt tacagatatg ccaatatcaa
     2581 cctggcacag ctgcaggaga acctgggagg agcatccagg gagcaggccc tggagatcgc
     2641 cacacacgtg gtgcacatgc tggcaaccga ggtgccagga gcaaagcagc gcacctacgc
     2701 cgccttcaat cctgccgata tggtcatggt gaacttttcc gacatgccac tgtctatggc
     2761 caatgccttc gagaaggccg tgaaggccaa ggatggcttc ctgcagcctt ccatccaggc
     2821 ctttaaccag tactgggacc gcgtggccaa tggatatggc ctgaacggag ctgccgccca
     2881 gttttccctg tctgatgtgg accctatcac agcccaggtg aagcagatgc caaccctgga
     2941 gcagctgaag agctgggtgc ggaacaatgg agaggcaaag cggactgctg atggcagtga
     3001 atttgagtcc actagtccaa agaagaagag aaaggtggga agcggagcaa ccaacttcag
     3061 cctgctgaag caggccggcg atgtggagga gaatccaggc cccatgccaa agaagaagag
     3121 aaaggtgacg cgtgaacgct cctacctgat cctgagactg gcaggaccaa tgcaggcatg
     3181 gggacagcct acattcgagg gaaccaggcc aacaggccgc tttcctaccc ggtctggact
     3241 gctgggactg ctgggagcct gcctgggcat ccagagggac gatacctcta gcctgcaggc
     3301 cctgagcgag tccgtgcagt tcgccgtgcg ctgtgatgag ctgatcctgg acgataggcg
     3361 cgtgtccgtg acaggcctgc gggattacca caccgtgctg ggcgccagag aggactatag
     3421 gggcctgaag tcccacgaga ccatccagac atggcgcgag tacctgtgcg acgcctcttt
     3481 tacagtggcc ctgtggctga ccccacacgc aacaatggtc atcagcgagc tggagaaggc
     3541 cgtgctgaag ccacggtaca ccccctatct gggccggaga agctgccctc tgacacaccc
     3601 actgttcctg ggcacctgtc aggcctccga tccccagaag gccctgctga actacgagcc
     3661 tgtgggcggc gacatctatt ctgaggagag cgtgacaggc caccacctga agttcaccgc
     3721 cagggatgag ccaatgatca cactgccaag gcagtttgca tccagggagt ggtatgtgat
     3781 caagggagga atggacgtga gccagaagcg gactgctgat ggcagtgaat ttgagtcctc
     3841 tagaccaaag aagaagagaa aggtgggctc cggcgagggc aggggaagtc ttctaacatg
     3901 cggggacgtg gaggaaaatc ccggcccaat gccaaagaag aagagaaagg tgggatccaa
     3961 cctgctgatt gacaactgga ttcctgtgcg cccacggaac ggaggaaaag tccagattat
     4021 taatctgcag agcctgtact gctcccggga tcagtggaga ctgagcctgc ccagagacga
     4081 tatggagctg gccgccctgg ccctgctggt gtgcatcggc cagatcatcg cccctgccaa
     4141 ggacgatgtg gagttcaggc accgcatcat gaaccctctg accgaggatg agtttcagca
     4201 gctgatcgcc ccatggatcg acatgttcta tctgaatcac gccgagcacc ccttcatgca
     4261 gacaaagggc gtgaaggcca acgacgtgac ccccatggag aagctgctgg caggcgtgtc
     4321 cggagcaaca aattgcgcct tcgtgaacca gccaggacag ggagaggccc tgtgcggagg
     4381 ctgtaccgcc atcgccctgt ttaatcaggc aaaccaggca cctggattcg gaggaggctt
     4441 taagtctgga ctgaggggag gaaccccagt gaccacattc gtgagaggca tcgatctgag
     4501 gagcacagtg ctgctgaatg tgctgaccct gccacggctg cagaagcagt ttcccaatga
     4561 gagccacaca gagaaccagc ccacctggat caagcctatc aagtctaacg agagcatccc
     4621 tgccagctcc atcggcttcg tgagaggcct gttttggcag ccagcccaca tcgagctgtg
     4681 cgaccccatc ggcatcggca agtgttcttg ctgtggccag gaaagcaatc tgaggtacac
     4741 cggcttcctg aaggagaagt tcacctttac agtgaacggc ctgtggcccc accctcactc
     4801 tccatgtctg gtgacagtga agaagggcga ggtggaggag aagttcctgg cctttaccac
     4861 atccgccccc tcttggaccc agatcagcag agtggtggtg gacaagatca tccagaacga
     4921 gaatggcaac agagtggccg ccgtggtgaa tcagttcagg aacatcgccc cacagtctcc
     4981 cctggagctg atcatgggcg gctacaggaa caatcaggcc agcatcctgg agcggagaca
     5041 cgatgtgctg atgtttaatc agggctggca gcagtatggc aatgtgatca acgagatcgt
     5101 gacagtgggc ctgggctaca agaccgccct gagaaaggcc ctgtatacat tcgccgaggg
     5161 ctttaagaac aaggacttca agggagcagg cgtgagcgtg cacgagaccg ccgagaggca
     5221 cttttaccgc cagtccgagc tgctgatccc cgatgtgctg gccaatgtga acttctccca
     5281 ggccgacgaa gtgatcgccg atctgaggga caagctgcac cagctgtgcg agatgctgtt
     5341 taaccagtct gtggccccat acgcccacca ccccaagctg atcagcacac tggccctggc
     5401 aagggccacc ctgtataagc acctgaggga gctgaagcca cagggaggac cttctaatgg
     5461 agctagccca aagaagaaga gaaaggtgta agcggccgca ctcgagatat ctagacccag
     5521 ctttcttgta caaagtggtg atctagctaa aacgcggcct cgaatcgaat tccgcccccc
     5581 cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     5641 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     5701 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     5761 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     5821 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     5881 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     5941 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     6001 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     6061 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
     6121 ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaaccatg gtgagcaagg
     6181 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     6241 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     6301 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
     6361 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     6421 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     6481 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     6541 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
     6601 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     6661 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     6721 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
     6781 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     6841 gccactccac cggcggcatg gacgagctgt acaagtaaag cgctctaggc ttaagtatac
     6901 ctcgagttaa attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg
     6961 ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg
     7021 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg
     7081 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc
     7141 atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg
     7201 ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg
     7261 tccattcctt attccataga aaagccttga cttgaggtta gatttttttt atattttgtt
     7321 ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat
     7381 ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc
     7441 tcgacttaat taaccaacaa gctcgtcatc gctttgcaga agagcagaga ggatatgctc
     7501 atcgtctaaa gaactaccca ttttattata tattagtcac gatatctata acaagaaaat
     7561 atatatataa taagttatca cgtaagtaga acatgaaata acaatataat tatcgtatga
     7621 gttaaatctt aaaagtcacg taaaagataa tcatgcgtca ttttgactca cgcggtcgtt
     7681 atagttcaaa atcagtgaca cttaccgcat tgacaagcac gcctcacggg agctccaagc
     7741 ggcgactgag atgtcctaaa tgcacagcga cggattcgcg ctatttagaa agagagagca
     7801 atatttcaag aatgcatgcg tcaattttac gcagactatc tttctagggt taatacgtat
     7861 aatacatatg attcagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7921 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7981 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8041 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8101 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8161 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8221 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8281 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8341 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8401 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8461 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8521 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     8581 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8641 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8701 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8761 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8821 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8881 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8941 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9001 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9061 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9121 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9181 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9241 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9301 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9361 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9421 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9481 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9541 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9601 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9661 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9721 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9781 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9841 atttcccc
//
