LOCUS       sequence-267593-       10358 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Components for genome editing in mammalian cells with 
            pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA and pBS-U6-crRNA-targeted. This 
            plasmid was optimized for iPS cells and coexpresses mCherry 
            protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10358)
  AUTHORS   Morisaka H, Yoshimi K, Okuzaki Y, Gee P, Kunihiro Y, Sonpho E, Xu H,
            Sasakawa N, Naito Y, Nakada S, Yamamoto T, Sano S, Hotta A, Takeda 
            J, Mashimo T
  TITLE     CRISPR-Cas3 induces broad and unidirectional genome editing in human
            cells.
  JOURNAL   Nat Commun. 2019 Dec 6;10(1):5302. doi: 10.1038/s41467-019-13226-x.
  PUBMED    31811138
REFERENCE   2  (bases 1 to 10358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-6"; volume: "10(1):5302. doi"; pages: " 
            10.1038/s41467-019-13226-x"
COMMENT     Sequence Label: pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA
FEATURES             Location/Qualifiers
     source          1..10358
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   46..108
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        578..1756
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          809..1747
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1704..1724
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1779..1803
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1850..1859
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1862..1882
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2408..2428
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2438..2494
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2498..2518
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3161..3181
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3191..3244
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3248..3268
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5978..5998
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(6025..6049)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(6091..6665,6666..6668,6669..6677)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6258..6275)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6485..6504
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6678..7388
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6823..6841)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7091..7110
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(7502..7521)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7567..7622
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7621..7640)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     repeat_region   8325..8359
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8452..8469)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8623..9211)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8703..8722)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9382..10173,10174..10242))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10005..10024
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10243..10347)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaaaagtgcc acctgacgtc atctgttaac attatacgcg tttaacccta gaaagataat
       61 catattgtga cgtacgttaa agataatcat gcgtaaaatt gacgcatgtg ttttatcggt
      121 ctgtatatcg aggtttattt attaatttga atagatatta agttttatta tatttacact
      181 tacatactaa taataaattc aacaaacaat ttatttatgt ttatttattt attaaaaaaa
      241 aacaaaaact caaaatttct tctataaagt aacaaaactt ttaaacattc tctcttttac
      301 aaaaataaac ttattttgta ctttaaaaac agtcatgttg tattataaaa taagtaatta
      361 gcttaaccta tacataatag aaacaaatta tacttattag tcagtcagaa acaactttgg
      421 cacatatcaa tattatgctc tgctagcgtt taaacccggg cgatcgctct agactagtgc
      481 ggccgctgca gaagctttgc aaagatggat aaagttttaa acagagagga atctttgcag
      541 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      601 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      661 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      721 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      781 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      841 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      901 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      961 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
     1021 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
     1081 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
     1141 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
     1201 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
     1261 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
     1321 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
     1381 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
     1441 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1501 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1561 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1621 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1681 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1741 atttcaggtg tcgtgaggaa ttagcggtgg cggccatcac aagtttgtac aaaaaagcag
     1801 gctggcgccg gaaccaattc agtcgactgg atccggtacc gaattcgccg ccaccatggc
     1861 cccaaagaag aagagaaagg tgagatctga agccgatgag atcgacgcaa tggcactgta
     1921 cagggcatgg cagcagctgg acaacggatc ttgcgcacag atcaggcgcg tgagcgagcc
     1981 tgatgagctg agggacatcc cagccttcta tcggctggtg cagccctttg gctgggagaa
     2041 tcctagacac cagcaggccc tgctgaggat ggtgttttgt ctgagcgccg gcaagaacgt
     2101 gatccggcac caggacaaga agagcgagca gaccacagga atctccctgg gacgcgccct
     2161 ggccaattcc ggccggatca acgagcggag aatcttccag ctgatcaggg ccgatcgcac
     2221 agccgacatg gtgcagctga ggcgcctgct gacccacgca gagcctgtgc tggattggcc
     2281 actgatggcc cgcatgctga catggtgggg caagcgggag agacagcagc tgctggagga
     2341 cttcgtgctg accacaaata agaacgccaa gcggactgct gatggcagtg aatttgagtc
     2401 cactagtcca aagaagaaga gaaaggtggg aagcggagca accaacttca gcctgctgaa
     2461 gcaggccggc gatgtggagg agaatccagg ccccatgcca aagaagaaga gaaaggtgac
     2521 gcgtgaatac ctgagcaaag tgatcatcgc aagggcatgg tccagggacc tgtatcagct
     2581 gcaccagggc ctgtggcacc tgttccctaa tagaccagat gccgccaggg acttcctgtt
     2641 tcacgtggag aagaggaaca cacccgaggg ctgtcacgtg ctgctgcagt ccgcccagat
     2701 gcccgtgagc accgcagtgg ccacagtgat caagaccaag caggtggagt tccagctgca
     2761 agtgggcgtg ccactgtact ttaggctgcg cgccaatccc atcaagacca tcctggataa
     2821 ccagaagcgc ctggactcta agggcaatat caagcggtgc agagtgcctc tgatcaagga
     2881 ggccgagcag atcgcctggc tgcagagaaa gctgggcaac gccgccaggg tggaggatgt
     2941 gcaccctatc agcgagcggc cacagtattt cagcggcgac ggcaagtccg gcaagatcca
     3001 gaccgtgtgc tttgagggcg tgctgaccat caacgatgcc ccagccctga tcgacctggt
     3061 gcagcaggga atcggacctg ctaagtcaat gggatgtggg ctgctgtcac tggcacctct
     3121 gaagcggact gctgatggca gtgaatttga gtcctctaga ccaaagaaga agagaaaggt
     3181 gggctccggc gagggcaggg gaagtcttct aacatgcggg gacgtggagg aaaatcccgg
     3241 cccaatgcca aagaagaaga gaaaggtggg atccgaggag cccttcaagt acatctgcca
     3301 ctattggggc aagagctcca agagcctgac aaagggcaac gatatccacc tgctgatcta
     3361 ccactgcctg gacgtggcag cagtggcaga ttgttggtgg gaccagtccg tggtgctgca
     3421 gaataccttt tgtcggaacg agatgctgtc taagcagaga gtgaaggcct ggctgctgtt
     3481 ctttatcgcc ctgcacgata tcggcaagtt cgacatccgg tttcagtata agtccgccga
     3541 gtcttggctg aagctgaatc cagccacacc ctctctgaac ggccctagca cccagatgtg
     3601 cagaaagttc aatcacggcg ccgccggcct gtactggttt aaccaggact ctctgagcga
     3661 gcagagcctg ggcgatttct tttccttctt tgacgcagca cctcacccct acgagtcctg
     3721 gttcccttgg gtggaggcag tgaccggaca ccacggcttt atcctgcatt ctcaggacca
     3781 ggataagagc cggtgggaga tgccagccag cctggcctcc tacgccgcac aggataagca
     3841 ggccagagag gagtggatca gcgtgctgga ggccctgttc ctgaccccag caggactgtc
     3901 catcaatgat atcccacctg actgctctag cctgctggcc ggcttttgtt ccctggccga
     3961 ctggctgggc tcttggacca caaccaatac attcctgttt aacgaggatg caccaagcga
     4021 catcaacgcc ctgaggacct acttccagga tcggcagcag gacgcctcca gagtgctgga
     4081 gctgtccggc ctggtgtcta ataagaggtg ctacgaggga gtgcacgccc tgctggataa
     4141 cggctatcag ccaaggcagc tgcaggtgct ggtggacgcc ctgcctgtgg caccaggact
     4201 gacagtgatc gaggcaccta ccggcagcgg caagacagag accgccctgg cctatgcctg
     4261 gaagctgatc gatcagcaga tcgccgactc cgtgatcttc gccctgccaa cacaggccac
     4321 cgccaatgca atgctgaccc ggatggaggc ctccgcctct cacctgtttt cctctcccaa
     4381 cctgatcctg gcccacggca acagcaggtt caatcacctg tttcagtcta tcaagagccg
     4441 cgccatcaca gagcagggac aggaggaggc ctgggtgcag tgctgtcagt ggctgagcca
     4501 gtccaataag aaggtgttcc tgggccagat cggcgtgtgc accatcgatc aggtgctgat
     4561 ctctgtgctg cctgtgaagc acaggtttat caggggactg ggaatcggca gaagcgtgct
     4621 gatcgtggat gaggtgcacg cctacgacac atatatgaac ggcctgctgg aggccgtgct
     4681 gaaggcacag gcagacgtgg gcggctccgt gatcctgctg tctgccaccc tgccaatgaa
     4741 gcagaagcag aagctgctgg atacatacgg cctgcacacc gaccccgtgg agaacaattc
     4801 cgcctatcct ctgatcaatt ggaggggagt gaacggagca cagcggttcg acctgctggc
     4861 ccaccctgag cagctgccac caaggttttc tatccagccc gagcctatct gcctggccga
     4921 tatgctgcca gacctgacca tgctggagag gatgatcgct gccgccaatg caggagcaca
     4981 ggtgtgcctg atctgtaacc tggtggatgt ggcccaggtg tgctaccaga ggctgaagga
     5041 gctgaacaat acacaggtgg acatcgatct gttccacgcc cggtttaccc tgaatgaccg
     5101 gagagagaag gagaacagag tgatctctaa cttcggcaag aatggcaaga gaaacgtggg
     5161 cagaatcctg gtggccacac aggtggtgga gcagagcctg gacgtggatt tcgactggct
     5221 gatcacccag cactgccccg ccgatctgct gtttcagcgg ctgggcagac tgcacaggca
     5281 ccaccgcaag tatcggcctg ccggctttga gatcccagtg gccacaatcc tgctgcctga
     5341 cggagaggga tacggcaggc acgagcacat ctatagcaat gtgcgcgtga tgtggcggac
     5401 ccagcagcac atcgaggagc tgaacggcgc ctctctgttc tttccagatg cctaccgcca
     5461 gtggctggac agcatctatg acgatgccga gatggatgag cccgagtggg tgggcaatgg
     5521 catggacaag ttcgagtccg ccgagtgtga gaagcggttc aaggccagga aggtgctgca
     5581 gtgggccgag gagtactccc tgcaggataa cgacgagaca atcctggccg tgaccagaga
     5641 tggcgagatg tctctgcccc tgctgcctta tgtgcagaca agctccggca agcagctgct
     5701 ggatggccag gtgtacgagg acctgagcca cgagcagcag tatgaggccc tggccctgaa
     5761 cagggtgaat gtgcccttca cctggaagcg gagcttcagc gaagtggtgg atgaggacgg
     5821 cctgctgtgg ctggagggca agcagaatct ggatggctgg gtgtggcagg gcaactccat
     5881 cgtgatcaca tacaccggcg acgagggcat gaccagagtg atcccagcca accccaagaa
     5941 gagaaccgcc gacggcagcg agttcgagtc cgctagccca aagaagaaga gaaaggtgta
     6001 agcggccgca ctcgagatat ctagacccag ctttcttgta caaagtggtg atctagctaa
     6061 aacgcggcct cgaatcgaat tccgcccccc cccctctccc tccccccccc ctaacgttac
     6121 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
     6181 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
     6241 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
     6301 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
     6361 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
     6421 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
     6481 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     6541 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
     6601 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
     6661 ataatatggc cacaaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
     6721 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     6781 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     6841 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
     6901 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     6961 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     7021 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     7081 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
     7141 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     7201 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     7261 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
     7321 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     7381 acaagtaaag cgctctaggc ttaagtatac ctcgagttaa attcactcct caggtgcagg
     7441 ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag
     7501 atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt
     7561 ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct
     7621 cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt
     7681 agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag
     7741 gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga
     7801 cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa
     7861 aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca
     7921 tagctgtccc tcttctctta tggagatccc tcgacttaat taaccaacaa gctcgtcatc
     7981 gctttgcaga agagcagaga ggatatgctc atcgtctaaa gaactaccca ttttattata
     8041 tattagtcac gatatctata acaagaaaat atatatataa taagttatca cgtaagtaga
     8101 acatgaaata acaatataat tatcgtatga gttaaatctt aaaagtcacg taaaagataa
     8161 tcatgcgtca ttttgactca cgcggtcgtt atagttcaaa atcagtgaca cttaccgcat
     8221 tgacaagcac gcctcacggg agctccaagc ggcgactgag atgtcctaaa tgcacagcga
     8281 cggattcgcg ctatttagaa agagagagca atatttcaag aatgcatgcg tcaattttac
     8341 gcagactatc tttctagggt taatacgtat aatacatatg attcagctgc attaatgaat
     8401 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     8461 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     8521 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8581 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8641 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8701 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8761 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8821 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8881 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8941 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     9001 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     9061 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     9121 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     9181 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     9241 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     9301 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     9361 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     9421 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9481 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     9541 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     9601 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9661 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     9721 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9781 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     9841 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     9901 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9961 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10021 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    10081 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    10141 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    10201 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    10261 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    10321 gaaaaataaa caaatagggg ttccgcgcac atttcccc
//
