LOCUS       sequence-267600-        9811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Components for genome editing in mammalian cells with 
            pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA and pBS-U6-crRNA-targeted. This 
            plasmid was optimized for iPS cells and coexpresses PuroR protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Morisaka H, Yoshimi K, Okuzaki Y, Gee P, Kunihiro Y, Sonpho E, Xu H,
            Sasakawa N, Naito Y, Nakada S, Yamamoto T, Sano S, Hotta A, Takeda 
            J, Mashimo T
  TITLE     CRISPR-Cas3 induces broad and unidirectional genome editing in human
            cells.
  JOURNAL   Nat Commun. 2019 Dec 6;10(1):5302. doi: 10.1038/s41467-019-13226-x.
  PUBMED    31811138
REFERENCE   2  (bases 1 to 9811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-6"; volume: "10(1):5302. doi"; pages: " 
            10.1038/s41467-019-13226-x"
COMMENT     Sequence Label: pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA
FEATURES             Location/Qualifiers
     source          1..9811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   46..108
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        578..1756
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          809..1747
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1704..1724
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1779..1803
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1850..1859
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1862..1882
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3017..3037
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3047..3103
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3107..3127
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3845..3865
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3875..3928
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3932..3952
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5468..5488
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(5515..5539)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(5581..6155,6156..6158,6159..6167)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5748..5765)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5975..5994
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6168..6767
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6168..6187)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6664..6684
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(6955..6974)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7020..7075
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7074..7093)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     repeat_region   7778..7812
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7905..7922)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8076..8664)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8156..8175)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8835..9626,9627..9695))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9458..9477
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9696..9800)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaaaagtgcc acctgacgtc atctgttaac attatacgcg tttaacccta gaaagataat
       61 catattgtga cgtacgttaa agataatcat gcgtaaaatt gacgcatgtg ttttatcggt
      121 ctgtatatcg aggtttattt attaatttga atagatatta agttttatta tatttacact
      181 tacatactaa taataaattc aacaaacaat ttatttatgt ttatttattt attaaaaaaa
      241 aacaaaaact caaaatttct tctataaagt aacaaaactt ttaaacattc tctcttttac
      301 aaaaataaac ttattttgta ctttaaaaac agtcatgttg tattataaaa taagtaatta
      361 gcttaaccta tacataatag aaacaaatta tacttattag tcagtcagaa acaactttgg
      421 cacatatcaa tattatgctc tgctagcgtt taaacccggg cgatcgctct agactagtgc
      481 ggccgctgca gaagctttgc aaagatggat aaagttttaa acagagagga atctttgcag
      541 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      601 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      661 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      721 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      781 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      841 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      901 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      961 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
     1021 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
     1081 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
     1141 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
     1201 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
     1261 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
     1321 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
     1381 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
     1441 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1501 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1561 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1621 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1681 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1741 atttcaggtg tcgtgaggaa ttagcggtgg cggccatcac aagtttgtac aaaaaagcag
     1801 gctggcgccg gaaccaattc agtcgactgg atccggtacc gaattcgccg ccaccatggc
     1861 cccaaagaag aagagaaagg tgagatctga atccaatttc atcaacatcc acgtgctgat
     1921 ctcccactct ccaagctgcc tgaatagaga cgatatgaac atgcagaagg acgccatctt
     1981 tggcggcaag cggagagtga ggatctctag ccagtccctg aagcgggcca tgagaaagtc
     2041 tggctactat gcccagaata tcggcgagtc ctctctgagg accatccacc tggcacagct
     2101 gagggacgtg ctgagacaga agctgggcga gcggtttgat cagaagatca tcgacaagac
     2161 actggccctg ctgagcggca agtccgtgga tgaggccgag aagatcagcg ccgacgcagt
     2221 gaccccatgg gtggtgggag agatcgcatg gttctgtgag caggtggcca aggccgaggc
     2281 cgataatctg gacgataaga agctgctgaa ggtgctgaag gaggatatcg ccgccatcag
     2341 agtgaacctg cagcagggag tggacatcgc cctgagcggc aggatggcca catccggcat
     2401 gatgaccgag ctgggcaagg tggacggagc aatgtccatc gcacacgcca tcaccacaca
     2461 ccaggtggac tctgatatcg actggttcac agccgtggac gatctgcagg agcagggaag
     2521 cgcccacctg ggaacccagg agttcagctc cggcgtgttt tacagatatg ccaatatcaa
     2581 cctggcacag ctgcaggaga acctgggagg agcatccagg gagcaggccc tggagatcgc
     2641 cacacacgtg gtgcacatgc tggcaaccga ggtgccagga gcaaagcagc gcacctacgc
     2701 cgccttcaat cctgccgata tggtcatggt gaacttttcc gacatgccac tgtctatggc
     2761 caatgccttc gagaaggccg tgaaggccaa ggatggcttc ctgcagcctt ccatccaggc
     2821 ctttaaccag tactgggacc gcgtggccaa tggatatggc ctgaacggag ctgccgccca
     2881 gttttccctg tctgatgtgg accctatcac agcccaggtg aagcagatgc caaccctgga
     2941 gcagctgaag agctgggtgc ggaacaatgg agaggcaaag cggactgctg atggcagtga
     3001 atttgagtcc actagtccaa agaagaagag aaaggtggga agcggagcaa ccaacttcag
     3061 cctgctgaag caggccggcg atgtggagga gaatccaggc cccatgccaa agaagaagag
     3121 aaaggtgacg cgtgaacgct cctacctgat cctgagactg gcaggaccaa tgcaggcatg
     3181 gggacagcct acattcgagg gaaccaggcc aacaggccgc tttcctaccc ggtctggact
     3241 gctgggactg ctgggagcct gcctgggcat ccagagggac gatacctcta gcctgcaggc
     3301 cctgagcgag tccgtgcagt tcgccgtgcg ctgtgatgag ctgatcctgg acgataggcg
     3361 cgtgtccgtg acaggcctgc gggattacca caccgtgctg ggcgccagag aggactatag
     3421 gggcctgaag tcccacgaga ccatccagac atggcgcgag tacctgtgcg acgcctcttt
     3481 tacagtggcc ctgtggctga ccccacacgc aacaatggtc atcagcgagc tggagaaggc
     3541 cgtgctgaag ccacggtaca ccccctatct gggccggaga agctgccctc tgacacaccc
     3601 actgttcctg ggcacctgtc aggcctccga tccccagaag gccctgctga actacgagcc
     3661 tgtgggcggc gacatctatt ctgaggagag cgtgacaggc caccacctga agttcaccgc
     3721 cagggatgag ccaatgatca cactgccaag gcagtttgca tccagggagt ggtatgtgat
     3781 caagggagga atggacgtga gccagaagcg gactgctgat ggcagtgaat ttgagtcctc
     3841 tagaccaaag aagaagagaa aggtgggctc cggcgagggc aggggaagtc ttctaacatg
     3901 cggggacgtg gaggaaaatc ccggcccaat gccaaagaag aagagaaagg tgggatccaa
     3961 cctgctgatt gacaactgga ttcctgtgcg cccacggaac ggaggaaaag tccagattat
     4021 taatctgcag agcctgtact gctcccggga tcagtggaga ctgagcctgc ccagagacga
     4081 tatggagctg gccgccctgg ccctgctggt gtgcatcggc cagatcatcg cccctgccaa
     4141 ggacgatgtg gagttcaggc accgcatcat gaaccctctg accgaggatg agtttcagca
     4201 gctgatcgcc ccatggatcg acatgttcta tctgaatcac gccgagcacc ccttcatgca
     4261 gacaaagggc gtgaaggcca acgacgtgac ccccatggag aagctgctgg caggcgtgtc
     4321 cggagcaaca aattgcgcct tcgtgaacca gccaggacag ggagaggccc tgtgcggagg
     4381 ctgtaccgcc atcgccctgt ttaatcaggc aaaccaggca cctggattcg gaggaggctt
     4441 taagtctgga ctgaggggag gaaccccagt gaccacattc gtgagaggca tcgatctgag
     4501 gagcacagtg ctgctgaatg tgctgaccct gccacggctg cagaagcagt ttcccaatga
     4561 gagccacaca gagaaccagc ccacctggat caagcctatc aagtctaacg agagcatccc
     4621 tgccagctcc atcggcttcg tgagaggcct gttttggcag ccagcccaca tcgagctgtg
     4681 cgaccccatc ggcatcggca agtgttcttg ctgtggccag gaaagcaatc tgaggtacac
     4741 cggcttcctg aaggagaagt tcacctttac agtgaacggc ctgtggcccc accctcactc
     4801 tccatgtctg gtgacagtga agaagggcga ggtggaggag aagttcctgg cctttaccac
     4861 atccgccccc tcttggaccc agatcagcag agtggtggtg gacaagatca tccagaacga
     4921 gaatggcaac agagtggccg ccgtggtgaa tcagttcagg aacatcgccc cacagtctcc
     4981 cctggagctg atcatgggcg gctacaggaa caatcaggcc agcatcctgg agcggagaca
     5041 cgatgtgctg atgtttaatc agggctggca gcagtatggc aatgtgatca acgagatcgt
     5101 gacagtgggc ctgggctaca agaccgccct gagaaaggcc ctgtatacat tcgccgaggg
     5161 ctttaagaac aaggacttca agggagcagg cgtgagcgtg cacgagaccg ccgagaggca
     5221 cttttaccgc cagtccgagc tgctgatccc cgatgtgctg gccaatgtga acttctccca
     5281 ggccgacgaa gtgatcgccg atctgaggga caagctgcac cagctgtgcg agatgctgtt
     5341 taaccagtct gtggccccat acgcccacca ccccaagctg atcagcacac tggccctggc
     5401 aagggccacc ctgtataagc acctgaggga gctgaagcca cagggaggac cttctaatgg
     5461 agctagccca aagaagaaga gaaaggtgta agcggccgca ctcgagatat ctagacccag
     5521 ctttcttgta caaagtggtg atctagctaa aacgcggcct cgaatcgaat tccgcccccc
     5581 cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     5641 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     5701 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     5761 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     5821 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     5881 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     5941 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     6001 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     6061 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
     6121 ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaaccatg accgagtaca
     6181 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     6241 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     6301 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     6361 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     6421 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     6481 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     6541 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     6601 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     6661 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     6721 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg
     6781 acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atcgattcga atgtacagat
     6841 ctggatccta ggcttaagta tacctcgagt taaattcact cctcaggtgc aggctgccta
     6901 tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt
     6961 tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta
     7021 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg
     7081 aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt
     7141 ggcaacatat gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca
     7201 gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg
     7261 ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc
     7321 cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt
     7381 ccctcttctc ttatggagat ccctcgactt aattaaccaa caagctcgtc atcgctttgc
     7441 agaagagcag agaggatatg ctcatcgtct aaagaactac ccattttatt atatattagt
     7501 cacgatatct ataacaagaa aatatatata taataagtta tcacgtaagt agaacatgaa
     7561 ataacaatat aattatcgta tgagttaaat cttaaaagtc acgtaaaaga taatcatgcg
     7621 tcattttgac tcacgcggtc gttatagttc aaaatcagtg acacttaccg cattgacaag
     7681 cacgcctcac gggagctcca agcggcgact gagatgtcct aaatgcacag cgacggattc
     7741 gcgctattta gaaagagaga gcaatatttc aagaatgcat gcgtcaattt tacgcagact
     7801 atctttctag ggttaatacg tataatacat atgattcagc tgcattaatg aatcggccaa
     7861 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     7921 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7981 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     8041 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     8101 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     8161 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     8221 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     8281 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     8341 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8401 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     8461 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     8521 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     8581 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     8641 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     8701 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     8761 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8821 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8881 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8941 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     9001 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9061 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     9121 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     9181 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     9241 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     9301 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     9361 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     9421 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     9481 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     9541 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     9601 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     9661 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9721 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     9781 aaacaaatag gggttccgcg cacatttccc c
//
