LOCUS       sequence-267601-        9663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector for sgRNAs cloned into the BsaI sites and for 
            expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 
            fused to N-terminus of Cas9, linked to mCherry via a T2A peptide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9663)
  AUTHORS   Reuven N, Adler J, Broennimann K, Myers N, Shaul Y
  TITLE     Recruitment of DNA Repair MRN Complex by Intrinsically Disordered 
            Protein Domain Fused to Cas9 Improves Efficiency of CRISPR-Mediated 
            Genome Editing.
  JOURNAL   Biomolecules. 2019 Oct 8;9(10). pii: biom9100584. doi: 
            10.3390/biom9100584.
  PUBMED    31597252
REFERENCE   2  (bases 1 to 9663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biomolecules."; date: "2019-10-8"; volume: "9(10). pii: 
            biom9100584. doi"; pages: " 10.3390/biom9100584"
COMMENT     Sequence Label: pU6-(BsaI)_CBh-UN-Cas9-T2A-mCherry
FEATURES             Location/Qualifiers
     source          1..9663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          153..380
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      399..408
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             795..860
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             867..887
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             912..5012
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             912..5012
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5013..5060
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5064..5117
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5124..5834
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5269..5287)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(5859..5876)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5865..6072
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6081..6221
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6081..6210
                     /label=AAV2 ITR
     rep_origin      6296..6751
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6383..6402)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6593..6614
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6768..6787)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6887..6909
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6947..6965)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7033..7137
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7138..7206,7207..7998)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7356..7375)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8169..8757
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8658..8677
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8819..9067
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8819..8839
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8990..9009
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        9088..9163
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        9260..9545
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg
       61 cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc
      121 ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg
      181 tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc
      241 cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagctg agcaagaggt
      301 aagggtttaa gggatggttg gttggtgggg tattaatgtt taattacctg gagcacctgc
      361 ctgaaatcac tttttttcag gttggaccgg tgaagcttgc caccatggag tccacgggag
      421 gcccagcatg tccgccggga cgcaccgtga ctaagcgttc ctgggccctg gccgaggaca
      481 cccctcgtgg ccccgacagc ccccccaagc gcccccgccc taacagtctt ccgctaacaa
      541 ccaccttccg tcccctgccc cccccacccc agacgacgtc agctgtggac ccgagctccc
      601 attcgcccgt taacccccca cgtgatcagc acgccaccga caccgcagac gaaaagcccc
      661 gggccgcgtc gccggcactt tctgacgcct cagggcctcc gaccccagac attccgctat
      721 ctcctggggg cacccacgcc cgcgacccgg acgccgatcc cgactccccg gaccttgact
      781 ctggatccgt catggactat aaggaccacg acggagacta caaggatcat gatattgatt
      841 acaaagacga tgacgataag atggccccaa agaagaagcg gaaggtcggt atccacggag
      901 tcccagcagc cgacaagaag tacagcatcg gcctggacat cggcaccaac tctgtgggct
      961 gggccgtgat caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca
     1021 ccgaccggca cagcatcaag aagaacctga tcggagccct gctgttcgac agcggcgaaa
     1081 cagccgaggc cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc
     1141 ggatctgcta tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct
     1201 tccacagact ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca
     1261 tcttcggcaa catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc
     1321 tgagaaagaa actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc
     1381 tggcccacat gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca
     1441 acagcgacgt ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg
     1501 aaaaccccat caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca
     1561 agagcagacg gctggaaaat ctgatcgccc agctgcccgg cgagaagaag aatggcctgt
     1621 tcggaaacct gattgccctg agcctgggcc tgacccccaa cttcaagagc aacttcgacc
     1681 tggccgagga tgccaaactg cagctgagca aggacaccta cgacgacgac ctggacaacc
     1741 tgctggccca gatcggcgac cagtacgccg acctgtttct ggccgccaag aacctgtccg
     1801 acgccatcct gctgagcgac atcctgagag tgaacaccga gatcaccaag gcccccctga
     1861 gcgcctctat gatcaagaga tacgacgagc accaccagga cctgaccctg ctgaaagctc
     1921 tcgtgcggca gcagctgcct gagaagtaca aagagatttt cttcgaccag agcaagaacg
     1981 gctacgccgg ctacattgac ggcggagcca gccaggaaga gttctacaag ttcatcaagc
     2041 ccatcctgga aaagatggac ggcaccgagg aactgctcgt gaagctgaac agagaggacc
     2101 tgctgcggaa gcagcggacc ttcgacaacg gcagcatccc ccaccagatc cacctgggag
     2161 agctgcacgc cattctgcgg cggcaggaag atttttaccc attcctgaag gacaaccggg
     2221 aaaagatcga gaagatcctg accttccgca tcccctacta cgtgggccct ctggccaggg
     2281 gaaacagcag attcgcctgg atgaccagaa agagcgagga aaccatcacc ccctggaact
     2341 tcgaggaagt ggtggacaag ggcgcttccg cccagagctt catcgagcgg atgaccaact
     2401 tcgataagaa cctgcccaac gagaaggtgc tgcccaagca cagcctgctg tacgagtact
     2461 tcaccgtgta taacgagctg accaaagtga aatacgtgac cgagggaatg agaaagcccg
     2521 ccttcctgag cggcgagcag aaaaaggcca tcgtggacct gctgttcaag accaaccgga
     2581 aagtgaccgt gaagcagctg aaagaggact acttcaagaa aatcgagtgc ttcgactccg
     2641 tggaaatctc cggcgtggaa gatcggttca acgcctccct gggcacatac cacgatctgc
     2701 tgaaaattat caaggacaag gacttcctgg acaatgagga aaacgaggac attctggaag
     2761 atatcgtgct gaccctgaca ctgtttgagg acagagagat gatcgaggaa cggctgaaaa
     2821 cctatgccca cctgttcgac gacaaagtga tgaagcagct gaagcggcgg agatacaccg
     2881 gctggggcag gctgagccgg aagctgatca acggcatccg ggacaagcag tccggcaaga
     2941 caatcctgga tttcctgaag tccgacggct tcgccaacag aaacttcatg cagctgatcc
     3001 acgacgacag cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg
     3061 atagcctgca cgagcacatt gccaatctgg ccggcagccc cgccattaag aagggcatcc
     3121 tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat gggccggcac aagcccgaga
     3181 acatcgtgat cgaaatggcc agagagaacc agaccaccca gaagggacag aagaacagcc
     3241 gcgagagaat gaagcggatc gaagagggca tcaaagagct gggcagccag atcctgaaag
     3301 aacaccccgt ggaaaacacc cagctgcaga acgagaagct gtacctgtac tacctgcaga
     3361 atgggcggga tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc gactacgatg
     3421 tggaccatat cgtgcctcag agctttctga aggacgactc catcgacaac aaggtgctga
     3481 ccagaagcga caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga
     3541 agatgaagaa ctactggcgg cagctgctga acgccaagct gattacccag agaaagttcg
     3601 acaatctgac caaggccgag agaggcggcc tgagcgaact ggataaggcc ggcttcatca
     3661 agagacagct ggtggaaacc cggcagatca caaagcacgt ggcacagatc ctggactccc
     3721 ggatgaacac taagtacgac gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc
     3781 tgaagtccaa gctggtgtcc gatttccgga aggatttcca gttttacaaa gtgcgcgaga
     3841 tcaacaacta ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga accgccctga
     3901 tcaaaaagta ccctaagctg gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg
     3961 tgcggaagat gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc aagtacttct
     4021 tctacagcaa catcatgaac tttttcaaga ccgagattac cctggccaac ggcgagatcc
     4081 ggaagcggcc tctgatcgag acaaacggcg aaaccgggga gatcgtgtgg gataagggcc
     4141 gggattttgc caccgtgcgg aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga
     4201 ccgaggtgca gacaggcggc ttcagcaaag agtctatcct gcccaagagg aacagcgata
     4261 agctgatcgc cagaaagaag gactgggacc ctaagaagta cggcggcttc gacagcccca
     4321 ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc aagaaactga
     4381 agagtgtgaa agagctgctg gggatcacca tcatggaaag aagcagcttc gagaagaatc
     4441 ccatcgactt tctggaagcc aagggctaca aagaagtgaa aaaggacctg atcatcaagc
     4501 tgcctaagta ctccctgttc gagctggaaa acggccggaa gagaatgctg gcctctgccg
     4561 gcgaactgca gaagggaaac gaactggccc tgccctccaa atatgtgaac ttcctgtacc
     4621 tggccagcca ctatgagaag ctgaagggct cccccgagga taatgagcag aaacagctgt
     4681 ttgtggaaca gcacaagcac tacctggacg agatcatcga gcagatcagc gagttctcca
     4741 agagagtgat cctggccgac gctaatctgg acaaagtgct gtccgcctac aacaagcacc
     4801 gggataagcc catcagagag caggccgaga atatcatcca cctgtttacc ctgaccaatc
     4861 tgggagcccc tgccgccttc aagtactttg acaccaccat cgaccggaag aggtacacca
     4921 gcaccaaaga ggtgctggac gccaccctga tccaccagag catcaccggc ctgtacgaga
     4981 cacggatcga cctgtctcag ctgggaggcg acaaaaggcc ggcggccacg aaaaaggccg
     5041 gccaggcaaa aaagaaaaag cttgagggca gaggaagtct gctaacatgc ggtgacgtgg
     5101 aggagaatcc cggccctgct agcatggtga gcaagggcga ggaggataac atggccatca
     5161 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
     5221 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     5281 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
     5341 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
     5401 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
     5461 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
     5521 acttcccctc agacggcccc gtaatgcaga agaaaaccat gggctgggag gcctcctccg
     5581 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
     5641 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
     5701 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
     5761 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
     5821 agctgtacaa gtaagaattc ctagagctcg ctgatcagcc tcgactgtgc cttctagttg
     5881 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     5941 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     6001 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag agaatagcag
     6061 gcatgctggg gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg
     6121 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
     6181 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt
     6241 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
     6301 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     6361 cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     6421 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     6481 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
     6541 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     6601 ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga tttataaggg
     6661 attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     6721 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
     6781 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     6841 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     6901 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     6961 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     7021 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     7081 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     7141 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     7201 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     7261 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     7321 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     7381 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     7441 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     7501 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     7561 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     7621 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     7681 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     7741 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     7801 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tggaagccgc
     7861 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     7921 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     7981 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     8041 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     8101 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     8161 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     8221 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     8281 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     8341 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     8401 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     8461 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     8521 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     8581 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     8641 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     8701 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     8761 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtga
     8821 gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat
     8881 aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
     8941 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
     9001 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg
     9061 aaacaccgga gacctgtaca ggtctctgtt ttagagctag aaatagcaag ttaaaataag
     9121 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttg ttttagagct
     9181 agaaatagca agttaaaata aggctagtcc gtttttagcg cgtgcgccaa ttctgcagac
     9241 aaatggctct agaggtaccc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     9301 ccaacgaccc ccgcccattg acgtcaatag taacgccaat agggactttc cattgacgtc
     9361 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     9421 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tgtgcccagt
     9481 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     9541 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
     9601 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
     9661 ggg
//
