LOCUS       sequence-267605-       10321 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Components for genome editing in mammalian cells with 
            pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA and pBS-U6-crRNA-targeted. This 
            plasmid was optimized for iPS cells and coexpresses PuroR protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10321)
  AUTHORS   Morisaka H, Yoshimi K, Okuzaki Y, Gee P, Kunihiro Y, Sonpho E, Xu H,
            Sasakawa N, Naito Y, Nakada S, Yamamoto T, Sano S, Hotta A, Takeda 
            J, Mashimo T
  TITLE     CRISPR-Cas3 induces broad and unidirectional genome editing in human
            cells.
  JOURNAL   Nat Commun. 2019 Dec 6;10(1):5302. doi: 10.1038/s41467-019-13226-x.
  PUBMED    31811138
REFERENCE   2  (bases 1 to 10321)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-6"; volume: "10(1):5302. doi"; pages: " 
            10.1038/s41467-019-13226-x"
COMMENT     Sequence Label: pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA
FEATURES             Location/Qualifiers
     source          1..10321
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   46..108
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        578..1756
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          809..1747
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1704..1724
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1779..1803
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1850..1859
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1862..1882
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2408..2428
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2438..2494
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2498..2518
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3161..3181
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3191..3244
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3248..3268
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5978..5998
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(6025..6049)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(6091..6665,6666..6668,6669..6677)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6258..6275)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6485..6504
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6678..7277
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6678..6697)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7174..7194
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7465..7484)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7530..7585
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7584..7603)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     repeat_region   8288..8322
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8415..8432)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8586..9174)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8666..8685)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9345..10136,10137..10205))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9968..9987
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10206..10310)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaaaagtgcc acctgacgtc atctgttaac attatacgcg tttaacccta gaaagataat
       61 catattgtga cgtacgttaa agataatcat gcgtaaaatt gacgcatgtg ttttatcggt
      121 ctgtatatcg aggtttattt attaatttga atagatatta agttttatta tatttacact
      181 tacatactaa taataaattc aacaaacaat ttatttatgt ttatttattt attaaaaaaa
      241 aacaaaaact caaaatttct tctataaagt aacaaaactt ttaaacattc tctcttttac
      301 aaaaataaac ttattttgta ctttaaaaac agtcatgttg tattataaaa taagtaatta
      361 gcttaaccta tacataatag aaacaaatta tacttattag tcagtcagaa acaactttgg
      421 cacatatcaa tattatgctc tgctagcgtt taaacccggg cgatcgctct agactagtgc
      481 ggccgctgca gaagctttgc aaagatggat aaagttttaa acagagagga atctttgcag
      541 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      601 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      661 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      721 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      781 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      841 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      901 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      961 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
     1021 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
     1081 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
     1141 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
     1201 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
     1261 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
     1321 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
     1381 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
     1441 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1501 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1561 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1621 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1681 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1741 atttcaggtg tcgtgaggaa ttagcggtgg cggccatcac aagtttgtac aaaaaagcag
     1801 gctggcgccg gaaccaattc agtcgactgg atccggtacc gaattcgccg ccaccatggc
     1861 cccaaagaag aagagaaagg tgagatctga agccgatgag atcgacgcaa tggcactgta
     1921 cagggcatgg cagcagctgg acaacggatc ttgcgcacag atcaggcgcg tgagcgagcc
     1981 tgatgagctg agggacatcc cagccttcta tcggctggtg cagccctttg gctgggagaa
     2041 tcctagacac cagcaggccc tgctgaggat ggtgttttgt ctgagcgccg gcaagaacgt
     2101 gatccggcac caggacaaga agagcgagca gaccacagga atctccctgg gacgcgccct
     2161 ggccaattcc ggccggatca acgagcggag aatcttccag ctgatcaggg ccgatcgcac
     2221 agccgacatg gtgcagctga ggcgcctgct gacccacgca gagcctgtgc tggattggcc
     2281 actgatggcc cgcatgctga catggtgggg caagcgggag agacagcagc tgctggagga
     2341 cttcgtgctg accacaaata agaacgccaa gcggactgct gatggcagtg aatttgagtc
     2401 cactagtcca aagaagaaga gaaaggtggg aagcggagca accaacttca gcctgctgaa
     2461 gcaggccggc gatgtggagg agaatccagg ccccatgcca aagaagaaga gaaaggtgac
     2521 gcgtgaatac ctgagcaaag tgatcatcgc aagggcatgg tccagggacc tgtatcagct
     2581 gcaccagggc ctgtggcacc tgttccctaa tagaccagat gccgccaggg acttcctgtt
     2641 tcacgtggag aagaggaaca cacccgaggg ctgtcacgtg ctgctgcagt ccgcccagat
     2701 gcccgtgagc accgcagtgg ccacagtgat caagaccaag caggtggagt tccagctgca
     2761 agtgggcgtg ccactgtact ttaggctgcg cgccaatccc atcaagacca tcctggataa
     2821 ccagaagcgc ctggactcta agggcaatat caagcggtgc agagtgcctc tgatcaagga
     2881 ggccgagcag atcgcctggc tgcagagaaa gctgggcaac gccgccaggg tggaggatgt
     2941 gcaccctatc agcgagcggc cacagtattt cagcggcgac ggcaagtccg gcaagatcca
     3001 gaccgtgtgc tttgagggcg tgctgaccat caacgatgcc ccagccctga tcgacctggt
     3061 gcagcaggga atcggacctg ctaagtcaat gggatgtggg ctgctgtcac tggcacctct
     3121 gaagcggact gctgatggca gtgaatttga gtcctctaga ccaaagaaga agagaaaggt
     3181 gggctccggc gagggcaggg gaagtcttct aacatgcggg gacgtggagg aaaatcccgg
     3241 cccaatgcca aagaagaaga gaaaggtggg atccgaggag cccttcaagt acatctgcca
     3301 ctattggggc aagagctcca agagcctgac aaagggcaac gatatccacc tgctgatcta
     3361 ccactgcctg gacgtggcag cagtggcaga ttgttggtgg gaccagtccg tggtgctgca
     3421 gaataccttt tgtcggaacg agatgctgtc taagcagaga gtgaaggcct ggctgctgtt
     3481 ctttatcgcc ctgcacgata tcggcaagtt cgacatccgg tttcagtata agtccgccga
     3541 gtcttggctg aagctgaatc cagccacacc ctctctgaac ggccctagca cccagatgtg
     3601 cagaaagttc aatcacggcg ccgccggcct gtactggttt aaccaggact ctctgagcga
     3661 gcagagcctg ggcgatttct tttccttctt tgacgcagca cctcacccct acgagtcctg
     3721 gttcccttgg gtggaggcag tgaccggaca ccacggcttt atcctgcatt ctcaggacca
     3781 ggataagagc cggtgggaga tgccagccag cctggcctcc tacgccgcac aggataagca
     3841 ggccagagag gagtggatca gcgtgctgga ggccctgttc ctgaccccag caggactgtc
     3901 catcaatgat atcccacctg actgctctag cctgctggcc ggcttttgtt ccctggccga
     3961 ctggctgggc tcttggacca caaccaatac attcctgttt aacgaggatg caccaagcga
     4021 catcaacgcc ctgaggacct acttccagga tcggcagcag gacgcctcca gagtgctgga
     4081 gctgtccggc ctggtgtcta ataagaggtg ctacgaggga gtgcacgccc tgctggataa
     4141 cggctatcag ccaaggcagc tgcaggtgct ggtggacgcc ctgcctgtgg caccaggact
     4201 gacagtgatc gaggcaccta ccggcagcgg caagacagag accgccctgg cctatgcctg
     4261 gaagctgatc gatcagcaga tcgccgactc cgtgatcttc gccctgccaa cacaggccac
     4321 cgccaatgca atgctgaccc ggatggaggc ctccgcctct cacctgtttt cctctcccaa
     4381 cctgatcctg gcccacggca acagcaggtt caatcacctg tttcagtcta tcaagagccg
     4441 cgccatcaca gagcagggac aggaggaggc ctgggtgcag tgctgtcagt ggctgagcca
     4501 gtccaataag aaggtgttcc tgggccagat cggcgtgtgc accatcgatc aggtgctgat
     4561 ctctgtgctg cctgtgaagc acaggtttat caggggactg ggaatcggca gaagcgtgct
     4621 gatcgtggat gaggtgcacg cctacgacac atatatgaac ggcctgctgg aggccgtgct
     4681 gaaggcacag gcagacgtgg gcggctccgt gatcctgctg tctgccaccc tgccaatgaa
     4741 gcagaagcag aagctgctgg atacatacgg cctgcacacc gaccccgtgg agaacaattc
     4801 cgcctatcct ctgatcaatt ggaggggagt gaacggagca cagcggttcg acctgctggc
     4861 ccaccctgag cagctgccac caaggttttc tatccagccc gagcctatct gcctggccga
     4921 tatgctgcca gacctgacca tgctggagag gatgatcgct gccgccaatg caggagcaca
     4981 ggtgtgcctg atctgtaacc tggtggatgt ggcccaggtg tgctaccaga ggctgaagga
     5041 gctgaacaat acacaggtgg acatcgatct gttccacgcc cggtttaccc tgaatgaccg
     5101 gagagagaag gagaacagag tgatctctaa cttcggcaag aatggcaaga gaaacgtggg
     5161 cagaatcctg gtggccacac aggtggtgga gcagagcctg gacgtggatt tcgactggct
     5221 gatcacccag cactgccccg ccgatctgct gtttcagcgg ctgggcagac tgcacaggca
     5281 ccaccgcaag tatcggcctg ccggctttga gatcccagtg gccacaatcc tgctgcctga
     5341 cggagaggga tacggcaggc acgagcacat ctatagcaat gtgcgcgtga tgtggcggac
     5401 ccagcagcac atcgaggagc tgaacggcgc ctctctgttc tttccagatg cctaccgcca
     5461 gtggctggac agcatctatg acgatgccga gatggatgag cccgagtggg tgggcaatgg
     5521 catggacaag ttcgagtccg ccgagtgtga gaagcggttc aaggccagga aggtgctgca
     5581 gtgggccgag gagtactccc tgcaggataa cgacgagaca atcctggccg tgaccagaga
     5641 tggcgagatg tctctgcccc tgctgcctta tgtgcagaca agctccggca agcagctgct
     5701 ggatggccag gtgtacgagg acctgagcca cgagcagcag tatgaggccc tggccctgaa
     5761 cagggtgaat gtgcccttca cctggaagcg gagcttcagc gaagtggtgg atgaggacgg
     5821 cctgctgtgg ctggagggca agcagaatct ggatggctgg gtgtggcagg gcaactccat
     5881 cgtgatcaca tacaccggcg acgagggcat gaccagagtg atcccagcca accccaagaa
     5941 gagaaccgcc gacggcagcg agttcgagtc cgctagccca aagaagaaga gaaaggtgta
     6001 agcggccgca ctcgagatat ctagacccag ctttcttgta caaagtggtg atctagctaa
     6061 aacgcggcct cgaatcgaat tccgcccccc cccctctccc tccccccccc ctaacgttac
     6121 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
     6181 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
     6241 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
     6301 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
     6361 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
     6421 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
     6481 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     6541 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
     6601 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
     6661 ataatatggc cacaaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     6721 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     6781 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     6841 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     6901 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     6961 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     7021 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     7081 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     7141 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     7201 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     7261 gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac
     7321 gaccccatgc atcgattcga atgtacagat ctggatccta ggcttaagta tacctcgagt
     7381 taaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc
     7441 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
     7501 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     7561 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     7621 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
     7681 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     7741 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     7801 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
     7861 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgactt
     7921 aattaaccaa caagctcgtc atcgctttgc agaagagcag agaggatatg ctcatcgtct
     7981 aaagaactac ccattttatt atatattagt cacgatatct ataacaagaa aatatatata
     8041 taataagtta tcacgtaagt agaacatgaa ataacaatat aattatcgta tgagttaaat
     8101 cttaaaagtc acgtaaaaga taatcatgcg tcattttgac tcacgcggtc gttatagttc
     8161 aaaatcagtg acacttaccg cattgacaag cacgcctcac gggagctcca agcggcgact
     8221 gagatgtcct aaatgcacag cgacggattc gcgctattta gaaagagaga gcaatatttc
     8281 aagaatgcat gcgtcaattt tacgcagact atctttctag ggttaatacg tataatacat
     8341 atgattcagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     8401 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     8461 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     8521 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     8581 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     8641 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     8701 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     8761 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     8821 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     8881 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8941 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     9001 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     9061 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     9121 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     9181 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     9241 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     9301 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     9361 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     9421 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     9481 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     9541 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     9601 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     9661 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     9721 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     9781 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     9841 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     9901 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     9961 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    10021 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    10081 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    10141 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    10201 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    10261 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    10321 c
//
