LOCUS       sequence-267618-       11218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Piggybac vector for constitutive expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11218)
  AUTHORS   Fischer AD, Veronese-Paniagua DA, Swaminathan S, Kashima H, Rubin 
            DC, Madison BB
  TITLE     The oncogenic function of PLAGL2 is mediated via ASCL2 and IGF2 and 
            a Wnt-independent mechanism in colorectal cancer.
  JOURNAL   Am J Physiol Gastrointest Liver Physiol. 2023 Jun 13. doi: 
            10.1152/ajpgi.00058.2022.
  PUBMED    37310750
REFERENCE   2  (bases 1 to 11218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J 
            Physiol Gastrointest Liver Physiol. 2023 Jun 13. doi: 
            10.1152/ajpgi.00058.2022."
COMMENT     Sequence Label: BII-ChPtW-eSpCas9
FEATURES             Location/Qualifiers
     source          1..11218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    8..24
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13..35
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     32..48
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     32..48
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   539..573
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(842..858)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(842..858)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             2409..2462
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2469..2495
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2469..2495
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2508..2528
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2553..5090,5091..5093,5094..5555,5556..5558,5559..5726,
                     5727..5729,5730..6653)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system, mutated to improve targeting 
                     specificity (Slaymaker et al., 2016)"
                     /label=eSpCas9(1.1)
                     /note="carries the mutations K848A, K1003A, and R1060A"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLADDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPALESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKAPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6654..6701
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     misc_feature    6728..7316
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6781..6801)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7199..7210)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    7396..7530
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7420..7439)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7474..7493
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   8309..8371
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8501..8518)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8501..8517)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8510..8532)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8659..9114
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8746..8765)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8956..8977
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9140..9244
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9245..9313,9314..10105)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9463..9482)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10276..10864
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10765..10784
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11018..11035
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11152..11173
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11188..11193,11194..11211,11212..11218)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
       61 aagcgcgccc gccgggtaac tcacggggta tccatgtcca tttctgcggc atccagccag
      121 gatacccgtc ctcgctgacg taatatccca gcgccgcacc gctgtcatta atctgcacac
      181 cggcacggca gttccggctg tcgccggtat tgttcgggtt gctgatgcgc ttcgggctga
      241 ccatccggaa ctgtgtccgg aaaagccgcg acgaactggt atcccaggtg gcctgaacga
      301 acagttcacc gttaaaggcg tgcatggcca caccttcccg aatcatcatg gtaaacgtgc
      361 gttttcgctc aacgtcaatg cagcagcagt catcctcggc aaactctttc catgccgctt
      421 caacctcgcg ggaaaaggca cgggcttctt cctccccgat gcccagatag cgccagcttg
      481 ggcgatgact gagccggaaa aaagacccga cgatatgatc ctgatgcagc tagattaacc
      541 ctagaaagat agtctgcgta aaattgacgc atgcattctt gaaatattgc tctctctttc
      601 taaatagcgc gaatccgtcg ctgtgcattt aggacatctc agtcgccgct tggagctccc
      661 gtgaggcgtg cttgtcaatg cggtaagtgt cactgatttt gaactataac gaccgcgtga
      721 gtcaaaatga cgcatgatta tcttttacgt gacttttaag atttaactca tacgataatt
      781 atattgttat ttcatgttct acttacgtga taacttatta tatatatatt ttcttgttat
      841 agatccacta gttctagagg gacagccccc ccccaaagcc cccagggatg taattacgtc
      901 cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc
      961 cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg
     1021 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac
     1081 ggggaaaagg cctccaaggc ctgcaggagt caatgggaaa aacccattgg agccaagtac
     1141 actgactcaa tagggacttt ccattgggtt ttgcccagta cataaggtca atagggggtg
     1201 agtcaacagg aaagtcccat tggagccaag tacattgagt caatagggac tttccaatgg
     1261 gttttgccca gtacataagg tcaatgggag gtaagccaat gggtttttcc cattactgac
     1321 atgtatactg agtcattagg gactttccaa tgggttttgc ccagtacata aggtcaatag
     1381 gggtgaatca acaggaaagt cccattggag ccaagtacac tgagtcaata gggactttcc
     1441 attgggtttt gcccagtaca aaaggtcaat agggggtgag tcaatgggtt tttcccatta
     1501 ttggcacata cataaggtca ataggggtga ctagtggaga agagcatgct tgagggctga
     1561 gtgcccctca gtgggcagag agcacatggc ccacagtccc tgagaagttg gggggagggg
     1621 tgggcaattg aactggtgcc tagagaaggt ggggcttggg taaactggga aagtgatgtg
     1681 gtgtactggc tccacctttt tccccagggt gggggagaac catatataag tgcagtagtc
     1741 tctgtgaaca ttcggtttag tgaacctgca ggtcggccgc cacgaccggt gccaccatga
     1801 ctgaatacaa accaactgtt cgcctggcaa ctcgtgatga tgttccacgt gcagttcgca
     1861 ccctggctgc tgcatttgct gactaccctg caacccgtca cactgtggac ccagaccgcc
     1921 acattgaacg tgtgactgaa ctgcaggagc tgttcctgac ccgtgtgggc ctggacattg
     1981 gcaaagtgtg ggtggcagat gatggtgctg ctgtggcagt gtggaccacc cctgaatctg
     2041 ttgaagctgg tgcagtgttt gctgagattg gcccacgcat ggcagaactg tctggcagcc
     2101 gcctggcagc acaacagcag atggaaggtc tgctggcacc acaccgccca aaagaacctg
     2161 cttggttcct ggcaactgtg ggtgtgagcc ctgaccacca gggtaagggc ctgggctctg
     2221 cagtggtgct gcctggtgtg gaagcagctg aacgtgcagg tgtgcctgct ttcctggaga
     2281 cctcagctcc acgcaacctg cctttctatg aacgcctggg cttcactgtg actgctgatg
     2341 tggaagtgcc agaaggccca cgcacttggt gcatgactcg caaaccaggt gctagccttg
     2401 gctccggcga gggcagggga agtcttctaa catgcgggga cgtggaggaa aatcccggcc
     2461 cactcggcta cccctacgac gtgcccgact acgccagcgg catggcccca aagaagaagc
     2521 ggaaggtcgg tatccacgga gtcccagcag ccgacaagaa gtacagcatc ggcctggaca
     2581 tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg cccagcaaga
     2641 aattcaaggt gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggagccc
     2701 tgctgttcga cagcggcgaa acagccgagg ccacccggct gaagagaacc gccagaagaa
     2761 gatacaccag acggaagaac cggatctgct atctgcaaga gatcttcagc aacgagatgg
     2821 ccaaggtgga cgacagcttc ttccacagac tggaagagtc cttcctggtg gaagaggata
     2881 agaagcacga gcggcacccc atcttcggca acatcgtgga cgaggtggcc taccacgaga
     2941 agtaccccac catctaccac ctgagaaaga aactggtgga cagcaccgac aaggccgacc
     3001 tgcggctgat ctatctggcc ctggcccaca tgatcaagtt ccggggccac ttcctgatcg
     3061 agggcgacct gaaccccgac aacagcgacg tggacaagct gttcatccag ctggtgcaga
     3121 cctacaacca gctgttcgag gaaaacccca tcaacgccag cggcgtggac gccaaggcca
     3181 tcctgtctgc cagactgagc aagagcagac ggctggaaaa tctgatcgcc cagctgcccg
     3241 gcgagaagaa gaatggcctg ttcggaaacc tgattgccct gagcctgggc ctgaccccca
     3301 acttcaagag caacttcgac ctggccgagg atgccaaact gcagctgagc aaggacacct
     3361 acgacgacga cctggacaac ctgctggccc agatcggcga ccagtacgcc gacctgtttc
     3421 tggccgccaa gaacctgtcc gacgccatcc tgctgagcga catcctgaga gtgaacaccg
     3481 agatcaccaa ggcccccctg agcgcctcta tgatcaagag atacgacgag caccaccagg
     3541 acctgaccct gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac aaagagattt
     3601 tcttcgacca gagcaagaac ggctacgccg gctacattga cggcggagcc agccaggaag
     3661 agttctacaa gttcatcaag cccatcctgg aaaagatgga cggcaccgag gaactgctcg
     3721 tgaagctgaa cagagaggac ctgctgcgga agcagcggac cttcgacaac ggcagcatcc
     3781 cccaccagat ccacctggga gagctgcacg ccattctgcg gcggcaggaa gatttttacc
     3841 cattcctgaa ggacaaccgg gaaaagatcg agaagatcct gaccttccgc atcccctact
     3901 acgtgggccc tctggccagg ggaaacagca gattcgcctg gatgaccaga aagagcgagg
     3961 aaaccatcac cccctggaac ttcgaggaag tggtggacaa gggcgcttcc gcccagagct
     4021 tcatcgagcg gatgaccaac ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc
     4081 acagcctgct gtacgagtac ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga
     4141 ccgagggaat gagaaagccc gccttcctga gcggcgagca gaaaaaggcc atcgtggacc
     4201 tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac tacttcaaga
     4261 aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga agatcggttc aacgcctccc
     4321 tgggcacata ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg
     4381 aaaacgagga cattctggaa gatatcgtgc tgaccctgac actgtttgag gacagagaga
     4441 tgatcgagga acggctgaaa acctatgccc acctgttcga cgacaaagtg atgaagcagc
     4501 tgaagcggcg gagatacacc ggctggggca ggctgagccg gaagctgatc aacggcatcc
     4561 gggacaagca gtccggcaag acaatcctgg atttcctgaa gtccgacggc ttcgccaaca
     4621 gaaacttcat gcagctgatc cacgacgaca gcctgacctt taaagaggac atccagaaag
     4681 cccaggtgtc cggccagggc gatagcctgc acgagcacat tgccaatctg gccggcagcc
     4741 ccgccattaa gaagggcatc ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga
     4801 tgggccggca caagcccgag aacatcgtga tcgaaatggc cagagagaac cagaccaccc
     4861 agaagggaca gaagaacagc cgcgagagaa tgaagcggat cgaagagggc atcaaagagc
     4921 tgggcagcca gatcctgaaa gaacaccccg tggaaaacac ccagctgcag aacgagaagc
     4981 tgtacctgta ctacctgcag aatgggcggg atatgtacgt ggaccaggaa ctggacatca
     5041 accggctgtc cgactacgat gtggaccata tcgtgcctca gagctttctg gccgacgact
     5101 ccatcgacaa caaggtgctg accagaagcg acaagaaccg gggcaagagc gacaacgtgc
     5161 cctccgaaga ggtcgtgaag aagatgaaga actactggcg gcagctgctg aacgccaagc
     5221 tgattaccca gagaaagttc gacaatctga ccaaggccga gagaggcggc ctgagcgaac
     5281 tggataaggc cggcttcatc aagagacagc tggtggaaac ccggcagatc acaaagcacg
     5341 tggcacagat cctggactcc cggatgaaca ctaagtacga cgagaatgac aagctgatcc
     5401 gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc cgatttccgg aaggatttcc
     5461 agttttacaa agtgcgcgag atcaacaact accaccacgc ccacgacgcc tacctgaacg
     5521 ccgtcgtggg aaccgccctg atcaaaaagt accctgcgct ggaaagcgag ttcgtgtacg
     5581 gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca
     5641 aggctaccgc caagtacttc ttctacagca acatcatgaa ctttttcaag accgagatta
     5701 ccctggccaa cggcgagatc cggaaggcgc ctctgatcga gacaaacggc gaaaccgggg
     5761 agatcgtgtg ggataagggc cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc
     5821 aagtgaatat cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa gagtctatcc
     5881 tgcccaagag gaacagcgat aagctgatcg ccagaaagaa ggactgggac cctaagaagt
     5941 acggcggctt cgacagcccc accgtggcct attctgtgct ggtggtggcc aaagtggaaa
     6001 agggcaagtc caagaaactg aagagtgtga aagagctgct ggggatcacc atcatggaaa
     6061 gaagcagctt cgagaagaat cccatcgact ttctggaagc caagggctac aaagaagtga
     6121 aaaaggacct gatcatcaag ctgcctaagt actccctgtt cgagctggaa aacggccgga
     6181 agagaatgct ggcctctgcc ggcgaactgc agaagggaaa cgaactggcc ctgccctcca
     6241 aatatgtgaa cttcctgtac ctggccagcc actatgagaa gctgaagggc tcccccgagg
     6301 ataatgagca gaaacagctg tttgtggaac agcacaagca ctacctggac gagatcatcg
     6361 agcagatcag cgagttctcc aagagagtga tcctggccga cgctaatctg gacaaagtgc
     6421 tgtccgccta caacaagcac cgggataagc ccatcagaga gcaggccgag aatatcatcc
     6481 acctgtttac cctgaccaat ctgggagccc ctgccgcctt caagtacttt gacaccacca
     6541 tcgaccggaa gaggtacacc agcaccaaag aggtgctgga cgccaccctg atccaccaga
     6601 gcatcaccgg cctgtacgag acacggatcg acctgtctca gctgggaggc gacaaaaggc
     6661 cggcggccac gaaaaaggcc ggccaggcaa aaaagaaaaa gtaagagcta gagttcgcgt
     6721 ctggaacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6781 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     6841 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     6901 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     6961 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     7021 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     7081 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     7141 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     7201 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     7261 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     7321 aattaattct ggctaagcac ttcgtggccg tcgatcgttt aaagggaggt agtgagtcga
     7381 gatatcgata ctagtgatcc agacatgata agatacattg atgagtttgg acaaaccaca
     7441 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     7501 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     7561 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     7621 atggctgatt atgatcctct agagggacag ccccccccca aagcccccag ggatgtaatt
     7681 acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc cggtccggcg
     7741 ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc
     7801 acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga cacctggggg
     7861 gatacgggga aaaagccata ccaatggagc tcacggggac agcccccccc caaagccccc
     7921 agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc ggggctccgc
     7981 tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca gcccgggcac
     8041 ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt tgagcctgca
     8101 gacacctggg gggatacggg gaaaaagaat tcgataaaag ttttgttact ttatagaaga
     8161 aattttgagt ttttgttttt tttaataaat aaataaacat aaataaattg tttgttgaat
     8221 ttattattag tatgtaagtg taaatataat aaaacttaat atctattcaa attaataaat
     8281 aaacctcgat atacagaccg ataaaacaca tgcgtcaatt ttacacatga ttatctttaa
     8341 cgtacgtcac aatatgatta tctttctagg gttaatctag ctgcgtgttc tgcagcgtgt
     8401 cgagcatctt catctgctcc atcacgctgt aaaacacatt tgcaccgcga gtctgcccgt
     8461 cctccacggg ttcaaaaacg tgaatgaacg aggcgcgctc actggccgtc gttttacaac
     8521 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     8581 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     8641 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     8701 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     8761 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     8821 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     8881 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     8941 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     9001 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     9061 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     9121 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     9181 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     9241 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     9301 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     9361 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     9421 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     9481 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     9541 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     9601 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     9661 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     9721 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     9781 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     9841 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     9901 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     9961 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
    10021 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
    10081 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
    10141 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
    10201 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    10261 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    10321 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    10381 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
    10441 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    10501 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    10561 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    10621 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    10681 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    10741 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    10801 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    10861 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    10921 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
    10981 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
    11041 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
    11101 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
    11161 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttg
//
