LOCUS       sequence-267619-        9868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Piggybac vector for constitutive expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9868)
  AUTHORS   Fischer AD, Veronese-Paniagua DA, Swaminathan S, Kashima H, Rubin 
            DC, Madison BB
  TITLE     The oncogenic function of PLAGL2 is mediated via ASCL2 and IGF2 and 
            a Wnt-independent mechanism in colorectal cancer.
  JOURNAL   Am J Physiol Gastrointest Liver Physiol. 2023 Jun 13. doi: 
            10.1152/ajpgi.00058.2022.
  PUBMED    37310750
REFERENCE   2  (bases 1 to 9868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J 
            Physiol Gastrointest Liver Physiol. 2023 Jun 13. doi: 
            10.1152/ajpgi.00058.2022."
COMMENT     Sequence Label: BII-ChBtW-dnTCF4-ires-GFP
FEATURES             Location/Qualifiers
     source          1..9868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(269..285)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(269..285)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     regulatory      1218..1227
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1224..1619
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             1638..1691
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1701..1724
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1743..1766
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3448..4014
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3464..4016
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3608..3625)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3835..3854
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4028..4030,4031..4033,4034..4744)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4073..4094)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4334..4353)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4681..4702
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4748..4768
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    4805..5393
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4858..4878)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5276..5287)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5473..5607
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5497..5516)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5551..5570
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   6386..6448
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6578..6595)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6578..6594)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6587..6609)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6736..7191
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6823..6842)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7033..7054
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7217..7321
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7322..7390,7391..8182)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7540..7559)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8353..8941
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8842..8861
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9095..9112
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9229..9250
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9265..9270,9271..9288,9289..9295)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9303..9319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9308..9330
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9327..9343
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9327..9343
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   9834..9868
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 cattcttgaa atattgctct ctctttctaa atagcgcgaa tccgtcgctg tgcatttagg
       61 acatctcagt cgccgcttgg agctcccgtg aggcgtgctt gtcaatgcgg taagtgtcac
      121 tgattttgaa ctataacgac cgcgtgagtc aaaatgacgc atgattatct tttacgtgac
      181 ttttaagatt taactcatac gataattata ttgttatttc atgttctact tacgtgataa
      241 cttattatat atatattttc ttgttataga tccactagtt ctagagggac agcccccccc
      301 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
      361 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
      421 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
      481 tgagcctgca gacacctggg gggatacggg gaaaaggcct ccaaggcctg caggagtcaa
      541 tgggaaaaac ccattggagc caagtacact gactcaatag ggactttcca ttgggttttg
      601 cccagtacat aaggtcaata gggggtgagt caacaggaaa gtcccattgg agccaagtac
      661 attgagtcaa tagggacttt ccaatgggtt ttgcccagta cataaggtca atgggaggta
      721 agccaatggg tttttcccat tactgacatg tatactgagt cattagggac tttccaatgg
      781 gttttgccca gtacataagg tcaatagggg tgaatcaaca ggaaagtccc attggagcca
      841 agtacactga gtcaataggg actttccatt gggttttgcc cagtacaaaa ggtcaatagg
      901 gggtgagtca atgggttttt cccattattg gcacatacat aaggtcaata ggggtgacta
      961 gtggagaaga gcatgcttga gggctgagtg cccctcagtg ggcagagagc acatggccca
     1021 cagtccctga gaagttgggg ggaggggtgg gcaattgaac tggtgcctag agaaggtggg
     1081 gcttgggtaa actgggaaag tgatgtggtg tactggctcc acctttttcc ccagggtggg
     1141 ggagaaccat atataagtgc agtagtctct gtgaacattc ggtttagtga acctgcaggt
     1201 cggccgccac gaccggtgcc accatggcca agcctttgtc tcaagaagaa tccaccctca
     1261 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca
     1321 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg
     1381 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc
     1441 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt
     1501 gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg
     1561 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggcg
     1621 ctagccttgg ctccggcgag ggcaggggaa gtcttctaac atgcggggac gtggaggaaa
     1681 atcccggccc actcggcatg gactacaagg acgacgatga caagctcgat agcggcatgg
     1741 ctgactacaa ggacgacgat gacaagtcca tggctgctga tgtcaaatcg tctctagtca
     1801 atgaatcaga aacgaatcaa aacagctcct ccgattccga ggcggaaaga cggcctccgc
     1861 ctcgctccga aagtttccga gacaaatccc gggaaagttt ggaagaagcg gccaagaggc
     1921 aagatggagg gctctttaag gggccaccgt atcccggcta ccccttcatc atgatccccg
     1981 acctgacgag cccctacctc cccaacggat cgctctcgcc caccgcccga acctatctcc
     2041 agatgaaatg gccactgctt gatgtccagg cagggagcct ccagagtaga caagccctca
     2101 aggatgcccg gtccccatca ccggcacaca ttgtctctaa caaagtgcca gtggtgcagc
     2161 accctcacca tgtccacccc ctcacgcctc ttatcacgta cagcaatgaa cacttcacgc
     2221 cgggaaaccc acctccacac ttaccagccg acgtagaccc caaaacagga atcccacggc
     2281 ctccgcaccc tccagatata tccccgtatt acccactatc gcctggcacc gtaggacaaa
     2341 tcccccatcc gctaggatgg ttagtaccac agcaaggtca accagtgtac ccaatcacga
     2401 caggaggatt cagacacccc taccccacag ctctgaccgt caatgcttcc atgtccaggt
     2461 tccctcccca tatggtccca ccacatcata cgctacacac gacgggcatt ccgcatccgg
     2521 ccatagtcac accaacagtc aaacaggaat cgtcccagag tgatgtcggc tcactccata
     2581 gttcaaagca tcaggactcc aaaaaggaag aagaaaagaa gaagccccac ataaagaaac
     2641 ctcttaatgc attcatgttg tatatgaagg aaatgagagc aaaggtcgta gctgagtgca
     2701 cgttgaaaga aagcgcggcc atcaaccaga tccttgggcg gaggtggcat gcactgtcca
     2761 gagaagagca agcgaaatac tacgagctgg cccggaagga gcgacagctt catatgcaac
     2821 tgtaccccgg ctggtccgcg cgggataact atggaaagaa gaagaagagg aaaagggaca
     2881 agcagccggg agagaccaat gaacacagcg aatgtttcct aaatccttgc ctttcacttc
     2941 ctccgattac agacctgagc gctcctaaga aatgccgagc gcgctttggc cttgatcaac
     3001 agaataactg gtgcggccct tgcaggagaa aaaaaaagtg cgttcgctac atacaaggtg
     3061 aaggcagctg cctcagccca ccctcttcag atggaagctt actagattcg cctcccccct
     3121 ccccgaacct gctaggctcc cctccccgag acgccaagtc acagactgag cagacccagc
     3181 ctctgtcgct gtccctgaag cccgaccccc tggcccacct gtccatgatg cctccgccac
     3241 ccgccctcct gctcgctgag gccacccaca aggcctccgc cctctgtccc aacggggccc
     3301 tggacctgcc cccagccgct ttgcagcctg ccgccccctc ctcatcaatt gcacagccgt
     3361 cgacttcttc cttacattcc cacagctccc tggccgggac ccagccccag ccgctgtcgc
     3421 tcgtcaccaa gtctttagaa taggaattcc gccccccccc ctaacgttac tggccgaagc
     3481 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     3541 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     3601 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     3661 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     3721 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     3781 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     3841 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     3901 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     3961 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc
     4021 cacaaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     4081 gctggacggc gacgtaaacg gccacaagtt cagcgtgtct ggcgagggcg agggcgatgc
     4141 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     4201 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     4261 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     4321 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     4381 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     4441 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     4501 gaagaacggc atcaaggcga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     4561 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     4621 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     4681 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     4741 caagggcccc aagaagaagc gcaaggtggg cgcctaagta caagtaagag ttcgcgtctg
     4801 gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     4861 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     4921 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     4981 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     5041 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     5101 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     5161 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     5221 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     5281 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     5341 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat
     5401 taattctggc taagcacttc gtggccgtcg atcgtttaaa gggaggtagt gagtcgagat
     5461 atcgatacta gtgatccaga catgataaga tacattgatg agtttggaca aaccacaact
     5521 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     5581 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     5641 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
     5701 gctgattatg atcctctaga gggacagccc ccccccaaag cccccaggga tgtaattacg
     5761 tccctccccc gctagggggc agcagcgagc cgcccggggc tccgctccgg tccggcgctc
     5821 cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg
     5881 ggatcgcttt cctctgaacg cttctcgctg ctctttgagc ctgcagacac ctggggggat
     5941 acggggaaaa agccatacca atggagctca cggggacagc ccccccccaa agcccccagg
     6001 gatgtaatta cgtccctccc ccgctagggg gcagcagcga gccgcccggg gctccgctcc
     6061 ggtccggcgc tccccccgca tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg
     6121 gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc tgctctttga gcctgcagac
     6181 acctgggggg atacggggaa aaagaattcg ataaaagttt tgttacttta tagaagaaat
     6241 tttgagtttt tgtttttttt aataaataaa taaacataaa taaattgttt gttgaattta
     6301 ttattagtat gtaagtgtaa atataataaa acttaatatc tattcaaatt aataaataaa
     6361 cctcgatata cagaccgata aaacacatgc gtcaatttta cacatgatta tctttaacgt
     6421 acgtcacaat atgattatct ttctagggtt aatctagctg cgtgttctgc agcgtgtcga
     6481 gcatcttcat ctgctccatc acgctgtaaa acacatttgc accgcgagtc tgcccgtcct
     6541 ccacgggttc aaaaacgtga atgaacgagg cgcgctcact ggccgtcgtt ttacaacgtc
     6601 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     6661 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     6721 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     6781 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6841 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     6901 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     6961 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     7021 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     7081 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     7141 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     7201 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     7261 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     7321 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     7381 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     7441 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     7501 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     7561 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     7621 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     7681 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     7741 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     7801 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     7861 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     7921 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     7981 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     8041 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     8101 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     8161 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     8221 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     8281 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     8341 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     8401 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     8461 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     8521 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     8581 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     8641 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     8701 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     8761 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     8821 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8881 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8941 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     9001 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     9061 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     9121 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     9181 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     9241 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     9301 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     9361 cgcccgccgg gtaactcacg gggtatccat gtccatttct gcggcatcca gccaggatac
     9421 ccgtcctcgc tgacgtaata tcccagcgcc gcaccgctgt cattaatctg cacaccggca
     9481 cggcagttcc ggctgtcgcc ggtattgttc gggttgctga tgcgcttcgg gctgaccatc
     9541 cggaactgtg tccggaaaag ccgcgacgaa ctggtatccc aggtggcctg aacgaacagt
     9601 tcaccgttaa aggcgtgcat ggccacacct tcccgaatca tcatggtaaa cgtgcgtttt
     9661 cgctcaacgt caatgcagca gcagtcatcc tcggcaaact ctttccatgc cgcttcaacc
     9721 tcgcgggaaa aggcacgggc ttcttcctcc ccgatgccca gatagcgcca gcttgggcga
     9781 tgactgagcc ggaaaaaaga cccgacgata tgatcctgat gcagctagat taaccctaga
     9841 aagatagtct gcgtaaaatt gacgcatg
//
