LOCUS       sequence-267624-       13014 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses N-terminally strep tagged Kaposi's Sarcoma Associated 
            Herpesvirus (KSHV) ORF64.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13014)
  AUTHORS   Davis ZH, Verschueren E, Jang GM, Kleffman K, Johnson JR, Park J, 
            Von Dollen J, Maher MC, Johnson T, Newton W, Jager S, Shales M, 
            Horner J, Hernandez RD, Krogan NJ, Glaunsinger BA
  TITLE     Global mapping of herpesvirus-host protein complexes reveals a 
            transcription strategy for late genes.
  JOURNAL   Mol Cell. 2015 Jan 22;57(2):349-60. doi: 
            10.1016/j.molcel.2014.11.026. Epub 2014 Dec 24.
  PUBMED    25544563
REFERENCE   2  (bases 1 to 13014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.11.026"; journalName: "Mol Cell"; date: 
            "2015-01-22- 22"; volume: "57"; issue: "2"; pages: "349-60"
COMMENT     Sequence Label: pCDNA4.TO-ORF64-2xNSTREP
FEATURES             Location/Qualifiers
     source          1..13014
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(43..62)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        234..613
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        614..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     768..788
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    819..837
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    840..858
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     868..892
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             990..1013
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             1050..1073
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     regulatory      5896..5905
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(9021..9038)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9027..9251
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9297..9725
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9384..9403)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9594..9615
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9734..9754)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9739..10068
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9919..10054
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9981..10000
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10116..10163
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10182..10556
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10686..10807
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10723..10742)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10777..10796
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10856..10872)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10856..10872)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10869..10891)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10880..10896
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10904..10910,10911..10928,10929..10934))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10949..10970
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11087..11104)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11258..11846)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11338..11357)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12017..12808,12809..12877))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12640..12659
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12878..12982)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
       61 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      421 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      481 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      541 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      601 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      721 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      781 taggcgtgta cggtgggagg tctatataag cagagctctc cctatcagtg atagagatct
      841 ccctatcagt gatagagatc gtcgacgagc tcgtttagtg aaccgtcaga tcgcctggag
      901 acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccggac
      961 tctagcgttt aaacttaagc ttaccaatgt ggagccaccc ccagttcgag aagggcggcg
     1021 gcagcggcgg cggcagcggc ggcggcagct ggagccaccc ccagttcgag aagggggcgg
     1081 ccatggcagc ccagcctctg tacatggagg gaatggcctc cacccaccaa gctaactgta
     1141 tattcggaga acatgctgga tcccagtgcc tcagcaactg cgtcatgtac ctggcgtcca
     1201 gctattataa cagcgaaacc cccctcgtcg acagagccag cctggacgat gtacttgaac
     1261 agggcatgag gctggacctc ctcctacgaa aatctggcat gctgggattt agacaatatg
     1321 cccaacttca tcacatcccc ggattcctcc gcacagacga ctgggccacc aagatcttcc
     1381 agtctccaga gttttatggg ctcatcggac aggacgcggc catccgcgag ccattcatcg
     1441 agtccttgag gtcggttttg agtcgaaact acgcgggcac ggtacagtac ctgatcatta
     1501 tctgccagtc caaagccgga gcaatcgtcg tcaaggacaa aacgtattac atgtttgacc
     1561 cccactgcat accaaacatc cccaacagtc ctgcacacgt cataaagact aacgacgttg
     1621 gcgttttatt accgtacata gccacacatg acactgaata caccgggtgc ttcctttact
     1681 ttatcccaca tgactacatc agcccagagc actacatcgc aaaccactac cgcaccattg
     1741 tgttcgaaga actccacggg cccagaatgg atatctcccg cggggtggaa tcatgctcca
     1801 tcaccgaaat cacgtcccct tctgtatccc ccgcgcctag tgaggcacca ttgcgcaggg
     1861 actccaccca atcacaagac gaaacgcgcc cgcgcagacc tcgcgtcgtc attcctcctt
     1921 acgatccgac agaccgccca cgaccgcctc accaagaccg cccgccagag caggcagcgg
     1981 gatacggtgg aaacaaagga cgcggcggta acaaaggacg cggcggaaag acgggacgtg
     2041 gcggaaatga aggacgcggt ggccaccagc caccagacga gcaccagccc ccacacatca
     2101 ccgcggaaca catggaccag tccgacggac aaggcgccga tggagacatg gatagtacac
     2161 ccgcaaatgg tgagacatcc gttacggaaa ccccgggccc cgaacccaat cccccagcac
     2221 ggcctgacag agagccaccg cccactcccc cggcgacccc aggcgccaca gcgctgctct
     2281 ctgacctaac tgccacaaga gggcagaaac gcaaattttc ctcgcttaaa gaatcttatc
     2341 ccatcgacag cccaccctct gacgacgatg atgtgtccca gccctcccaa caaacggctc
     2401 cggatactga agatatttgg attgacgacc cactcacacc cttgtaccca ctaacggata
     2461 caccatcttt cgacataacg gcggacgtca cacccgacaa cacccacccc gagaaagcag
     2521 cggacgggga ctttaccaac aagaccacaa gcacggatgc ggacaggtat gccagcgcca
     2581 gtcaggaatc gctgggcacc ctggtctcgc catacgattt tacaaacttg gatacactgc
     2641 tggcagagct gggccggttg ggaacggcac agcctatccc tgtaatcgtg gacagactga
     2701 catcgcgacc ttttcgagaa gccagcgctc tacaggctat ggataggata ctaacacacg
     2761 tggtcctaga atacggtctg gtttcgggtt acagcacagc tgccccatcc aaatgcaccc
     2821 acgtcctcca gtttttcatt ttgtggggcg aaaaactcgg cataccaacg gaggacgcaa
     2881 agacgctcct ggaaagcgca ctggagatcc ccgcaatgtg cgagatcgtc caacagggcc
     2941 ggttgaagga gcccacgttc tcccgccaca ttataagcaa gctaaacccc tgcttggaat
     3001 ccctacacgc cactagtcgt caggacttca agtccctgat acaggcattc aacgccgaag
     3061 ggattaggat cgcctcgcgt gagagggaga cgtccatggc cgaactgata gaaacgataa
     3121 ccgcccgcct taaaccaaat tttaacattg tctgtgcccg ccaggacgca caaaccattc
     3181 aagacggcgt cggtctcctc agggccgagg ttaacaagag aaacgcacag atagcccagg
     3241 aggctgcgta ttttgagaat ataatcacgg ccctctccac attccaacca cctccccaat
     3301 cgcaacagac gttcgaagtg ctgccggacc tcaaactgcg cacgctcgtg gagcacctga
     3361 ccctggttga ggcgcaggtg acaacgcaaa cggtggaaag tctacaggca tacctacaga
     3421 gcgctgccac tgctgagcat caccttacca acgtgcccaa cgtccacagt atactgtcta
     3481 acatatccaa cactctaaaa gttatagatt atgtaattcc aaaatttata ataaacaccg
     3541 atacactggc cccatataaa cagcagtttt catatctggg gggtgaactg gcatctatgt
     3601 tctcccttga ctggcctcac gcacctgcag aggcggtaga gccactaccc gtgctgactt
     3661 ctctgcgagg taaaatcgca gaggcgctga cgcgtcaaga aaacaaaaac gctgtagatc
     3721 aaattctaac cgacgccgaa ggcctcctta agaacattac cgatccaaac ggcgcacact
     3781 tccacgccca ggccgtatca attccagtgt tagaaaacta cgtacataac gcgggggtcc
     3841 ttctcaaggg cgaaaagagc gagaggttct cccggctgaa gaccgccatc caaaacctgg
     3901 tatcctccga atcatttatc accgtgaccc tacacagtac aaaccttgga aacctagtta
     3961 ccaacgtacc aaaacttggt gaggcgttca ccgggggccc gcacctcctg acaagcccgt
     4021 ccgtgagaca gtccctttcc accctgtgca caaccctgct gcgagatgcc ctggacgccc
     4081 tggaaaaaaa ggatccggcc cttcttggtg aggggaccac gttggcgctg gagacactcc
     4141 taggatacgg gtcggtgcag gactacaagg agacggtaca gataatatcc agccttgtgg
     4201 gcatccaaaa attagtcagg gaccagggcg cggacaagtg ggccactgcc gtgacaaggc
     4261 taactgacct caaatcaact ctggccacga ccgccatcga gacggctacg aaacggaaac
     4321 tatacagatt gatccaaagg gacctcaaag aggctcaaaa acacgagacc aatcgggcca
     4381 tggaggaatg gaagcagaaa gtactggctc ttgacaatgc gtctccggaa cgtgtcgcca
     4441 ccctcctgca acaggctccc accgcgaagg ctagagagtt tgcagagaag cacttcaaaa
     4501 tactactccc cgtacccgcg gacgcccccg tccaagcgtc tccaacgccg atggaataca
     4561 gcgccagccc cctcccggac ccaaaggata tagacagagc tacatccatc cacggggaac
     4621 aggcgtggaa gaagatacag caggcgttca aggatttcaa cttcgccgtc ctgcggcccg
     4681 ctgactggga tgccctggca gcggagtacc aacgccgtgg ttcgcccctt ccggcggccg
     4741 tgggtccagc gctctcaggg ttcctggaga cgatcctagg gacgctgaac gacatctaca
     4801 tggataagct ccgctccttt ctgcccgacg cgcagccttt tcaggcgccg cccttcgact
     4861 ggctaacgcc gtatcaggac caagtcagct ttttcttgcg caccataggg ctgccgctgg
     4921 tgcgagcgct ggccgacaag atcagcgtgc aggcactgag gcttagccac gcgctccagt
     4981 ccggcgattt gcagcaggcc acggtgggca cgcccctgga gctccctgcc acagagtacg
     5041 cgcgcatcgc ctccaacatg aagtccgtgt tcaacgacca cggacttcag gtgcgatcag
     5101 aggtcgcgga ttatgtggag gcccaacgag ccgacgcaca cacgccacac gtcccacgtc
     5161 caaagataca ggcaccaaag actctgattc cacatccgga cgcaatcgtc gcggacggac
     5221 tacccgcctt tcttaagacg tccctactgc agcaagaggc caaacttctg gcgctacagc
     5281 gggcggactt cgagtcgctc gagagcgaca tgcgcgccgc agaggcccag agaaaagcat
     5341 cgcgcgagga aacccagcgc aaaatggcac acgccatcac tcagctctta cagcaggcac
     5401 ccagtgcgat ctcggggcgc ccgctatcct tacaggaccc ggtgggcttc ctcgagggca
     5461 tcatatacga caaggtcctg gagcgcgaat cctacgagac gggtctcgag ggactgtcct
     5521 ggctcgagca gaccatcaag tccatcaccg tatacgctcc cgtagaggag aagcaaagaa
     5581 tgcacgtgct gctggacgag gtgaaaaagc agcgagcaaa cactgagacc gctctcgagc
     5641 tagaggccgc ggctacgcac ggcgacgacg ctagactcct gcagcgagcg gtcgatgagc
     5701 tgtcaccgtt gcgcgttaag ggggggaagg ccgcggtgga atcctggcgg cagaaaatcc
     5761 aaaccctgaa atccctggta caggaagcgg agcaggccgg cctcctgttg gccaccatag
     5821 acacggtggc cggccaggcc caggagacca tatcaccatc cacactccag ggactgtacc
     5881 aacagggaca ggaggccatg gcggccatta agcggtttag ggactcgccc cagctagctg
     5941 gcctgcagga aaagctggcc gagctacagc agtacgtcaa gtacaagaag cagtatctgg
     6001 aacactttga ggccacccaa agcgtagtgt ttacagcctt tccgctcaca caggaggtta
     6061 cgatcccagc cctgcattac gcgggacctt tcgacaactt ggagcggctc tcacgatacc
     6121 tacacatcgg ccagacgcag ccggctccgg gacagtggct cctgacactt cccacattcg
     6181 accccacgcg cccggcctgc gtcccagccg gcggccacga acccccgttg cacagacagg
     6241 tggtgttctc cagctttttg gaggcccaga tccgattagc gttgtccgta gcgggccccg
     6301 tgcctggacg gggtctgccc ggaacaccgc agatccgaag gggcgtggag gctgccgctt
     6361 gtttcctcca ccagtgggac gagatatctc gcctccttcc agaggtactg gacacctttt
     6421 tccacaacgc gccccttccc gcagagtctt cctccaatgc tttcctggcc atgtgcgtat
     6481 tgacgcacct tgtctaccta gctgggcgcg ccgtcttggg cccacgggag ccggagcacg
     6541 ccgccccgga cgcgtaccca agggaggtgg cgctggcccc gcgcgacctg acctaccttc
     6601 tactggccat gtggccatct tggatctcgg caattttgaa acagccttcg cacgcggagg
     6661 cggcgcacgc atgtcttgtc acgctgccaa caatgctcaa ggctgtgccg tacctcacgc
     6721 tggaagcctc agctggacca ctgccggcgg acatgcgcca cttcgccacg ccagaagcgc
     6781 gtctgttttt ccccgcgcga tggcaccacg tcaacgtgca ggagaaactg tggctgcgta
     6841 atgattttat gtcgctgtgt caccgttccc cggggcgcgc gcgcatagcc gtcttggtgt
     6901 gggccgtcac ttgcctagat cctgaggtaa taaggcagct gtggtccacc ttgcggcccc
     6961 ttactgcgga tgaatccgac acggcttctg gactgctgcg ggtgctagta gaaatggagt
     7021 ttggtccgcc gcccaagacg ccgcggcggg aggcggtggc gcccggcgca acactgccac
     7081 cgtaccccta cggccttgcc accggcgagc gcctggtcgg ccaggcgcag gaacgctctg
     7141 gcggcgctgg caagatgccg gtgtccgggt ttgagatagt tttaggcgca ctgctgttcc
     7201 gcgcccccct acgcattttc agcaccgcat caacccacag gatctcagat ttcgagggcg
     7261 gtttccagat actgactcct ctcctggact gttgcccaga tcgcgagcca ttcgcctccc
     7321 tggccgccgc accacgaagg acggtgccac tgggagaccc gtgcgccaac attcacaccc
     7381 ccgaagagat acagatcttt gcgcgtcaag ccgcctggct tcaatatacc ttcgcaaatt
     7441 accagatccc cagcaccgac aacccgatac cgatcgttgt gctaaacgct aacaataacc
     7501 ttgaaaacag ctacatccct cgcgatcgca aagcggaccc gctacgacca ttctatgtag
     7561 tccctctgaa gccgcagggt agatggcctg aaataatgac cacagcaaca accccctgcc
     7621 gcctaccgac atcgccagaa gaggcgggat cacagttcgc cagactcctt cagagccagg
     7681 tgagcgccac atggtctgac atcttctcca gggttcccga gcgcctcgct cccaatgcga
     7741 ctcagaagag ttcccagaca atgtcagaaa tccacgaggt cgccgccacg ccgccactca
     7801 caatcacccc aaataaaccg accggaaccc ctcacgtctc cccggaggct gatccaataa
     7861 cagaacgcaa acgcggacag cagccgaaga ttgtcgcgga caacatgcct agtcgtattc
     7921 tcccgtcgct accgaccccg aaacccagag agcctagaat cacgctaccc cacgcactgc
     7981 ccgttatatc acccccagca catcgcccgt cgcctatacc gcatctgcca gcaccgcagg
     8041 taacggagcc caaaggggtt ctccaaagca aacgtggaac tctcgtgctg cggcccgccg
     8101 cggtcattga cccacggaag cccgtctcgg caccgatcac gcgatatgag aggacggcgc
     8161 tccagccccc ccggactgag ggcgaaggcc ggcgccctcc cgacacgcaa cccgtcactt
     8221 taacctttcg tctcccacct accgcaccca ctcccgcaac tgcagcccta gaaaccaaaa
     8281 caactccccc atccacgccc ccacacgcca tagacattag cccaccacag acacctccca
     8341 tgtccacctc acctcacgcg agagacacaa gcccccccgc agaaaagcgg gccgcacccg
     8401 tcattcgagt aatggcgccc acgcaaccgt cgggagaggc aagagtcaag cgagtggaga
     8461 tcgaacaggg cctttccaca cgcaatgaag cccctcccct tgaacgctcg aatcacgccg
     8521 tgcccgccgt taccccaagg cgcaccgtag cccgcgaaat caggatcccg ccggagataa
     8581 aggcgggttg ggacactgca ccggacattc ctctgcccca cagctccccg gagtcatccc
     8641 caccgacttc cccccagcct atccgcgtgg atgataaatc gcctcttccc aacctcgtag
     8701 agagatacgc gcggggtttc ctggacacgc cctctgtaga ggtgatgtcc ctggaaaatc
     8761 aggacatcgc cgtggacccc ggactgctaa cccgccggat tccatccgtg gtgcccatgc
     8821 cccatccaat tatgtggtca cccatagtac ccatcagttt acaaaacaca gacatagaca
     8881 ctgcaaagat aacactgatt agttttatta gacgcatcaa acaaaaagtg gccgccctat
     8941 cggcgtccct ggcggagacg gttgacagaa taaagaagtg gtacttgtga ctagagggcc
     9001 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     9061 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     9121 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     9181 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     9241 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     9301 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     9361 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     9421 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     9481 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     9541 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     9601 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     9661 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     9721 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     9781 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     9841 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     9901 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     9961 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
    10021 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
    10081 gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt
    10141 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg
    10201 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc
    10261 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga
    10321 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt
    10381 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc
    10441 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg
    10501 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg
    10561 tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
    10621 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
    10681 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
    10741 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
    10801 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
    10861 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
    10921 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
    10981 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
    11041 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
    11101 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    11161 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
    11221 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    11281 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    11341 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    11401 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    11461 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    11521 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    11581 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    11641 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
    11701 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    11761 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    11821 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    11881 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    11941 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    12001 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    12061 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    12121 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    12181 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    12241 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    12301 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    12361 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    12421 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    12481 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    12541 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    12601 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    12661 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    12721 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    12781 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    12841 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    12901 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    12961 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcg
//
