LOCUS       sequence-267638-        9050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Piggybac vector for SRIRACCHA-mediated mutation enrichment 
            (surrogate target for HDR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9050)
  AUTHORS   Wen Y, Liao G, Pritchard T, Zhao TT, Connelly JP, Pruett-Miller SM, 
            Blanc V, Davidson NO, Madison BB
  TITLE     A stable but reversible integrated surrogate reporter for assaying 
            CRISPR/Cas9-stimulated homology-directed repair.
  JOURNAL   J Biol Chem. 2017 Apr 14;292(15):6148-6162. doi: 
            10.1074/jbc.M117.777722. Epub 2017 Feb 22.
  PUBMED    28228480
REFERENCE   2  (bases 1 to 9050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M117.777722"; journalName: "J Biol Chem"; date: 
            "2017-04-14- 14"; volume: "292"; issue: "15"; pages: "6148-6162"
COMMENT     Sequence Label: BII-C3HTK
FEATURES             Location/Qualifiers
     source          1..9050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     8..24
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8..24
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   515..549
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     CDS             2441..3460
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             2441..3460
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    3491..4057
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3507..4059
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3651..3668)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3878..3897
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4059..5189
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMIQTHVTTPGSIPTICDLARTFAREMGEAN"
     polyA_signal    5204..5338
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5228..5247)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5282..5301
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   6117..6179
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6309..6326)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6309..6325)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6318..6340)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6467..6922
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6554..6573)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6764..6785
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6948..7052
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7053..7121,7122..7913)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7271..7290)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8084..8672
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8573..8592
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8826..8843
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8960..8981
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8996..9001,9002..9019,9020..9026)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9034..9050
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(9039..9050,1..11)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcccgccg ggtaactcac
       61 ggggtatcca tgtccatttc tgcggcatcc agccaggata cccgtcctcg ctgacgtaat
      121 atcccagcgc cgcaccgctg tcattaatct gcacaccggc acggcagttc cggctgtcgc
      181 cggtattgtt cgggttgctg atgcgcttcg ggctgaccat ccggaactgt gtccggaaaa
      241 gccgcgacga actggtatcc caggtggcct gaacgaacag ttcaccgtta aaggcgtgca
      301 tggccacacc ttcccgaatc atcatggtaa acgtgcgttt tcgctcaacg tcaatgcagc
      361 agcagtcatc ctcggcaaac tctttccatg ccgcttcaac ctcgcgggaa aaggcacggg
      421 cttcttcctc cccgatgccc agatagcgcc agcttgggcg atgactgagc cggaaaaaag
      481 acccgacgat atgatcctga tgcagctaga ttaaccctag aaagatagtc tgcgtaaaat
      541 tgacgcatgc attcttgaaa tattgctctc tctttctaaa tagcgcgaat ccgtcgctgt
      601 gcatttagga catctcagtc gccgcttgga gctcccgtga ggcgtgcttg tcaatgcggt
      661 aagtgtcact gattttgaac tataacgacc gcgtgagtca aaatgacgca tgattatctt
      721 ttacgtgact tttaagattt aactcatacg ataattatat tgttatttca tgttctactt
      781 acgtgataac ttattatata tatattttct tgttatagat ctgtggaatg tgtgctagag
      841 ggacagcccc cccccaaagc ccccagggat gtaattacgt ccctcccccg ctagggggca
      901 gcagcgagcc gcccggggct ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc
      961 agcgtgcggg gacagcccgg gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc
     1021 ttctcgctgc tctttgagcc tgcagacacc tggggggata cggggaaaag gcctccaagg
     1081 cctgcaggag tcaatgggaa aaacccattg gagccaagta cactgactca atagggactt
     1141 tccattgggt tttgcccagt acataaggtc aatagggggt gagtcaacag gaaagtccca
     1201 ttggagccaa gtacattgag tcaataggga ctttccaatg ggttttgccc agtacataag
     1261 gtcaatggga ggtaagccaa tgggtttttc ccattactga catgtatact gagtcattag
     1321 ggactttcca atgggttttg cccagtacat aaggtcaata ggggtgaatc aacaggaaag
     1381 tcccattgga gccaagtaca ctgagtcaat agggactttc cattgggttt tgcccagtac
     1441 aaaaggtcaa tagggggtga gtcaatgggt ttttcccatt attggcacat acataaggtc
     1501 aataggggtg actagtggag aagagcatgc ttgagggctg agtgcccctc agtgggcaga
     1561 gagcacatgg cccacagtcc ctgagaagtt ggggggaggg gtgggcaatt gaactggtgc
     1621 ctagagaagg tggggcttgg gtaaactggg aaagtgatgt ggtgtactgg ctccaccttt
     1681 ttccccaggg tgggggagaa ccatatataa gtgcagtagt ctctgtgaac attcggttta
     1741 gtgaacctgc aggtcggccg ccacgaccgg tgccaccatg actgaataca aaccaactgt
     1801 tcgcctggca actcgtgatg atgttccacg tgcagttcgc accctggctg ctgcatttgc
     1861 tgactaccct gcaacccgtc acactgtgga cccagaccgc cacattgaac gtgtgactga
     1921 actgcaggag ctgttcctga cccgtgtggg cctggacatt ggcaaagtgt gggtggcaga
     1981 tgatggtgct gctgtggcag tgtggaccac ccctgaatct gttgaagctg gtgcagtgtt
     2041 tgctgagatt ggcccacgca tggcagaact gtctggcagc cgcctggcag cacaacagca
     2101 gatggaaggt ctgctggcac cacaccgccc aaaagaacct gcttggttcc tggcaactgt
     2161 gggtgtgagc cctgaccacc agggtaaggg cctgggctct gcagtggtgc tgcctggtgt
     2221 ggaagcagct gaacgtgcag gtgtgcctgc tttcctggag acctcagctc cacgcaacct
     2281 gcctttctat gaacgcctgg gcttcactgt gactgctgat gtggaagtgc cagaaggccc
     2341 acgcacttgg tgcatgactc gcaaaccagg tgctaggtga ccgcaataaa aagacagaat
     2401 aaaacgcacg ggtgttgggt cgtttgttca ctcggcaata aagcctgaac tcaccgcgac
     2461 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
     2521 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
     2581 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
     2641 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta
     2701 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
     2761 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
     2821 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
     2881 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
     2941 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
     3001 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
     3061 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
     3121 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
     3181 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
     3241 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
     3301 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
     3361 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
     3421 aaaccgacgc cccagcactc gtccgagggc aaaggaatag agcggccgct cgaggtgcac
     3481 gaattgcaat tccgcccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     3541 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     3601 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     3661 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     3721 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     3781 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     3841 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     3901 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     3961 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
     4021 cggggacgtg gttttccttt gaaaaacacg atgataatat ggcttcgtac cccggccatc
     4081 agcacgcgtc tgcgttcgac caggctgcgc gttctcgcgg ccatagcaac cgacgtacgg
     4141 cgttgcgccc tcgccggcag caagaagcca cggaagtccg cccggagcag aaaatgccca
     4201 cgctactgcg ggtttatata gacggtcccc acgggatggg gaaaaccacc accacgcaac
     4261 tgctggtggc cctgggttcg cgcgacgata tcgtctacgt acccgagccg atgacttact
     4321 ggcgggtgct gggggcttcc gagacaatcg cgaacatcta caccacacaa caccgccttg
     4381 accagggtga gatatcggcc ggggacgcgg cggtggtaat gacaagcgcc cagataacaa
     4441 tgggcatgcc ttatgccgtg accgacgccg ttctggctcc tcatatcggg ggggaggctg
     4501 ggagctcaca tgccccgccc ccggccctca ccctcatctt cgaccgccat cccatcgccg
     4561 ccctcctgtg ctacccggcc gcgcgatacc ttatgggcag catgaccccc caggccgtgc
     4621 tggcgttcgt ggccctcatc ccgccgacct tgcccggcac aaacatcgtg ttgggggccc
     4681 ttccggagga cagacacatc gaccgcctgg ccaaacgcca gcgccccggc gagcggcttg
     4741 acctggctat gctggccgcg attcgccgcg tttacgggct gcttgccaat acggtgcggt
     4801 atctgcaggg cggcgggtcg tggcgggagg attggggaca gctttcgggg acggccgtgc
     4861 cgccccaggg tgccgagccc cagagcaacg cgggcccacg accccatatc ggggacacgt
     4921 tatttaccct gtttcgggcc cccgagttgc tggcccccaa cggcgacctg tacaacgtgt
     4981 ttgcctgggc cttggacgtc ttggccaaac gcctccgtcc catgcacgtc tttatcctgg
     5041 attacgacca atcgcccgcc ggctgccggg acgccctgct gcaacttacc tccgggatga
     5101 tccagaccca cgtcaccacc ccaggctcca taccgacgat ctgcgacctg gcgcgcacgt
     5161 ttgcccggga gatgggggag gctaactgaa acacggaact agtgatccag acatgataag
     5221 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     5281 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     5341 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     5401 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctag agggacagcc
     5461 cccccccaaa gcccccaggg atgtaattac gtccctcccc cgctaggggg cagcagcgag
     5521 ccgcccgggg ctccgctccg gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg
     5581 gggacagccc gggcacgggg aaggtggcac gggatcgctt tcctctgaac gcttctcgct
     5641 gctctttgag cctgcagaca cctgggggga tacggggaaa aagccatacc aatggagctc
     5701 acggggacag ccccccccca aagcccccag ggatgtaatt acgtccctcc cccgctaggg
     5761 ggcagcagcg agccgcccgg ggctccgctc cggtccggcg ctccccccgc atccccgagc
     5821 cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc acgggatcgc tttcctctga
     5881 acgcttctcg ctgctctttg agcctgcaga cacctggggg gatacgggga aaaagaattc
     5941 gataaaagtt ttgttacttt atagaagaaa ttttgagttt ttgttttttt taataaataa
     6001 ataaacataa ataaattgtt tgttgaattt attattagta tgtaagtgta aatataataa
     6061 aacttaatat ctattcaaat taataaataa acctcgatat acagaccgat aaaacacatg
     6121 cgtcaatttt acacatgatt atctttaacg tacgtcacaa tatgattatc tttctagggt
     6181 taatctagct gcgtgttctg cagcgtgtcg agcatcttca tctgctccat cacgctgtaa
     6241 aacacatttg caccgcgagt ctgcccgtcc tccacgggtt caaaaacgtg aatgaacgag
     6301 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     6361 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     6421 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     6481 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     6541 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     6601 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     6661 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     6721 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     6781 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     6841 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     6901 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     6961 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     7021 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     7081 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     7141 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     7201 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     7261 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     7321 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     7381 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     7441 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     7501 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     7561 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     7621 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     7681 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     7741 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     7801 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     7861 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     7921 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     7981 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     8041 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     8101 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     8161 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     8221 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     8281 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     8341 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     8401 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     8461 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     8521 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     8581 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     8641 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     8701 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     8761 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     8821 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     8881 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     8941 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     9001 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
//
