LOCUS       sequence-267640-        8852 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Piggybac vector for SRIRACCHA-mediated mutation enrichment 
            (surrogate target for HDR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8852)
  AUTHORS   Wen Y, Liao G, Pritchard T, Zhao TT, Connelly JP, Pruett-Miller SM, 
            Blanc V, Davidson NO, Madison BB
  TITLE     A stable but reversible integrated surrogate reporter for assaying 
            CRISPR/Cas9-stimulated homology-directed repair.
  JOURNAL   J Biol Chem. 2017 Apr 14;292(15):6148-6162. doi: 
            10.1074/jbc.M117.777722. Epub 2017 Feb 22.
  PUBMED    28228480
REFERENCE   2  (bases 1 to 8852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M117.777722"; journalName: "J Biol Chem"; date: 
            "2017-04-14- 14"; volume: "292"; issue: "15"; pages: "6148-6162"
COMMENT     Sequence Label: BII-B3HTK
FEATURES             Location/Qualifiers
     source          1..8852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1223..1232
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1229..1624
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             1694..2713
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             1694..2713
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    2744..3310
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2760..3312
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2904..2921)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3131..3150
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3312..4442
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMIQTHVTTPGSIPTICDLARTFAREMGEAN"
     polyA_signal    4457..4591
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4481..4500)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4535..4554
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   5370..5432
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5562..5579)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5562..5578)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5571..5593)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5720..6175
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5807..5826)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6017..6038
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6201..6305
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6306..6374,6375..7166)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6524..6543)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7337..7925
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7826..7845
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8079..8096
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8213..8234
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8249..8254,8255..8272,8273..8279)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8287..8303
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8292..8314
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8311..8327
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8311..8327
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   8818..8852
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 cattcttgaa atattgctct ctctttctaa atagcgcgaa tccgtcgctg tgcatttagg
       61 acatctcagt cgccgcttgg agctcccgtg aggcgtgctt gtcaatgcgg taagtgtcac
      121 tgattttgaa ctataacgac cgcgtgagtc aaaatgacgc atgattatct tttacgtgac
      181 ttttaagatt taactcatac gataattata ttgttatttc atgttctact tacgtgataa
      241 cttattatat atatattttc ttgttataga tctgtggaat gtgtgctaga gggacagccc
      301 ccccccaaag cccccaggga tgtaattacg tccctccccc gctagggggc agcagcgagc
      361 cgcccggggc tccgctccgg tccggcgctc cccccgcatc cccgagccgg cagcgtgcgg
      421 ggacagcccg ggcacgggga aggtggcacg ggatcgcttt cctctgaacg cttctcgctg
      481 ctctttgagc ctgcagacac ctggggggat acggggaaaa ggcctccaag gcctgcagga
      541 gtcaatggga aaaacccatt ggagccaagt acactgactc aatagggact ttccattggg
      601 ttttgcccag tacataaggt caataggggg tgagtcaaca ggaaagtccc attggagcca
      661 agtacattga gtcaataggg actttccaat gggttttgcc cagtacataa ggtcaatggg
      721 aggtaagcca atgggttttt cccattactg acatgtatac tgagtcatta gggactttcc
      781 aatgggtttt gcccagtaca taaggtcaat aggggtgaat caacaggaaa gtcccattgg
      841 agccaagtac actgagtcaa tagggacttt ccattgggtt ttgcccagta caaaaggtca
      901 atagggggtg agtcaatggg tttttcccat tattggcaca tacataaggt caataggggt
      961 gactagtgga gaagagcatg cttgagggct gagtgcccct cagtgggcag agagcacatg
     1021 gcccacagtc cctgagaagt tggggggagg ggtgggcaat tgaactggtg cctagagaag
     1081 gtggggcttg ggtaaactgg gaaagtgatg tggtgtactg gctccacctt tttccccagg
     1141 gtgggggaga accatatata agtgcagtag tctctgtgaa cattcggttt agtgaacctg
     1201 caggtcggcc gccacgaccg gtgccaccat ggccaagcct ttgtctcaag aagaatccac
     1261 cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt
     1321 cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt
     1381 tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg
     1441 caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg
     1501 acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag
     1561 tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga
     1621 gggcgctagg tgaccgcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt
     1681 tcactcggca ataaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa
     1741 gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag
     1801 cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta
     1861 caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct
     1921 tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt
     1981 cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc
     2041 catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc
     2101 gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca
     2161 tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct
     2221 cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga
     2281 tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag
     2341 cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg
     2401 gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg
     2461 atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt
     2521 ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg
     2581 atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac
     2641 cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag
     2701 ggcaaaggaa tagagcggcc gctcgaggtg cacgaattgc aattccgccc ccccccctaa
     2761 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     2821 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     2881 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     2941 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     3001 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     3061 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     3121 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     3181 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     3241 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     3301 acgatgataa tatggcttcg taccccggcc atcagcacgc gtctgcgttc gaccaggctg
     3361 cgcgttctcg cggccatagc aaccgacgta cggcgttgcg ccctcgccgg cagcaagaag
     3421 ccacggaagt ccgcccggag cagaaaatgc ccacgctact gcgggtttat atagacggtc
     3481 cccacgggat ggggaaaacc accaccacgc aactgctggt ggccctgggt tcgcgcgacg
     3541 atatcgtcta cgtacccgag ccgatgactt actggcgggt gctgggggct tccgagacaa
     3601 tcgcgaacat ctacaccaca caacaccgcc ttgaccaggg tgagatatcg gccggggacg
     3661 cggcggtggt aatgacaagc gcccagataa caatgggcat gccttatgcc gtgaccgacg
     3721 ccgttctggc tcctcatatc gggggggagg ctgggagctc acatgccccg cccccggccc
     3781 tcaccctcat cttcgaccgc catcccatcg ccgccctcct gtgctacccg gccgcgcgat
     3841 accttatggg cagcatgacc ccccaggccg tgctggcgtt cgtggccctc atcccgccga
     3901 ccttgcccgg cacaaacatc gtgttggggg cccttccgga ggacagacac atcgaccgcc
     3961 tggccaaacg ccagcgcccc ggcgagcggc ttgacctggc tatgctggcc gcgattcgcc
     4021 gcgtttacgg gctgcttgcc aatacggtgc ggtatctgca gggcggcggg tcgtggcggg
     4081 aggattgggg acagctttcg gggacggccg tgccgcccca gggtgccgag ccccagagca
     4141 acgcgggccc acgaccccat atcggggaca cgttatttac cctgtttcgg gcccccgagt
     4201 tgctggcccc caacggcgac ctgtacaacg tgtttgcctg ggccttggac gtcttggcca
     4261 aacgcctccg tcccatgcac gtctttatcc tggattacga ccaatcgccc gccggctgcc
     4321 gggacgccct gctgcaactt acctccggga tgatccagac ccacgtcacc accccaggct
     4381 ccataccgac gatctgcgac ctggcgcgca cgtttgcccg ggagatgggg gaggctaact
     4441 gaaacacgga actagtgatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     4501 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     4561 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     4621 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     4681 tatggctgat tatgatcctc tagagggaca gccccccccc aaagccccca gggatgtaat
     4741 tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc
     4801 gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg
     4861 cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg
     4921 ggatacgggg aaaaagccat accaatggag ctcacgggga cagccccccc ccaaagcccc
     4981 cagggatgta attacgtccc tcccccgcta gggggcagca gcgagccgcc cggggctccg
     5041 ctccggtccg gcgctccccc cgcatccccg agccggcagc gtgcggggac agcccgggca
     5101 cggggaaggt ggcacgggat cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc
     5161 agacacctgg ggggatacgg ggaaaaagaa ttcgataaaa gttttgttac tttatagaag
     5221 aaattttgag tttttgtttt ttttaataaa taaataaaca taaataaatt gtttgttgaa
     5281 tttattatta gtatgtaagt gtaaatataa taaaacttaa tatctattca aattaataaa
     5341 taaacctcga tatacagacc gataaaacac atgcgtcaat tttacacatg attatcttta
     5401 acgtacgtca caatatgatt atctttctag ggttaatcta gctgcgtgtt ctgcagcgtg
     5461 tcgagcatct tcatctgctc catcacgctg taaaacacat ttgcaccgcg agtctgcccg
     5521 tcctccacgg gttcaaaaac gtgaatgaac gaggcgcgct cactggccgt cgttttacaa
     5581 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     5641 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     5701 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     5761 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     5821 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     5881 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     5941 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     6001 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     6061 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     6121 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     6181 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     6241 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     6301 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     6361 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     6421 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     6481 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     6541 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     6601 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     6661 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     6721 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     6781 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     6841 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     6901 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     6961 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     7021 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     7081 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     7141 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     7201 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     7261 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     7321 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     7381 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     7441 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     7501 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     7561 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7621 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7681 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     7741 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7801 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7861 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7921 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7981 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     8041 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     8101 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     8161 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     8221 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     8281 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     8341 agcgcgcccg ccgggtaact cacggggtat ccatgtccat ttctgcggca tccagccagg
     8401 atacccgtcc tcgctgacgt aatatcccag cgccgcaccg ctgtcattaa tctgcacacc
     8461 ggcacggcag ttccggctgt cgccggtatt gttcgggttg ctgatgcgct tcgggctgac
     8521 catccggaac tgtgtccgga aaagccgcga cgaactggta tcccaggtgg cctgaacgaa
     8581 cagttcaccg ttaaaggcgt gcatggccac accttcccga atcatcatgg taaacgtgcg
     8641 ttttcgctca acgtcaatgc agcagcagtc atcctcggca aactctttcc atgccgcttc
     8701 aacctcgcgg gaaaaggcac gggcttcttc ctccccgatg cccagatagc gccagcttgg
     8761 gcgatgactg agccggaaaa aagacccgac gatatgatcc tgatgcagct agattaaccc
     8821 tagaaagata gtctgcgtaa aattgacgca tg
//
