LOCUS       sequence-26767-e         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Yang Z, MacQuarrie KL, Analau E, Tyler AE, Dilworth FJ, Cao Y, Diede
            SJ, Tapscott SJ
  TITLE     MyoD and E-protein heterodimers switch rhabdomyosarcoma cells from 
            an arrested myoblast phase to a differentiated state.
  JOURNAL   Genes Dev. 2009 Mar 15. 23(6):694-707.
  PUBMED    19299559
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2009 Mar 15. 23(6):694-707."
COMMENT     Sequence Label: MyoD-cTAP
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(68..241)
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     CDS             complement(242..415)
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             complement(455..475)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(503..580)
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 ctggcnattc ctacgtacca ccacactggg atcatcaggt tgacttcccc gcggaattcg
       61 cgtctacttt cggcgcctga gcaccattta gctttttagc ttctgctaaa aggttagcgc
      121 tttggcttgg gtcatctttt aaactttgga tgaaggcgtt tcgttgttct tcgtttaagt
      181 taggtaaatg taagatctca tagaacgcgt tttgttgttc tttgttgaat ttgttgtcta
      241 ctttcggcgc ctgagcatca tttagctttt tagcttctgc taaaaggtta gcgctttggc
      301 ttgggtcatc ttttaaactt tggatgaagg cgtttcgttg ttcttcgttt aagttaggta
      361 aatgtaagat ctcatagaac gcgttttgtt gttctttgtt gaatttgttg tccacggctt
      421 catcgtgttg cgcaagagcc gcggttttga gctcaccctg aaaatacaaa ttctcgctag
      481 cagtagttgg aatatcataa tcaagtgccc cggaggatga gattttctta aagcggttgg
      541 ctgctgagac ggctatgaaa ttctttttcc atcttctctt ttccatggat ccaagcacct
      601 gataaatcgc attggggttt gagcctgcag gacactgang ggcggcgtcg ggagangggg
      661 tctgggttcc ctgttctgtg tcgcttnggg atgccccctc tggcggaccc ggnncgactc
      721 tggtggtgca tctgccaaaa gcagcgcnnn gcancggggc tgtctgtgga gat
//
