LOCUS       sequence-26769-e         696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Yang Z, MacQuarrie KL, Analau E, Tyler AE, Dilworth FJ, Cao Y, Diede
            SJ, Tapscott SJ
  TITLE     MyoD and E-protein heterodimers switch rhabdomyosarcoma cells from 
            an arrested myoblast phase to a differentiated state.
  JOURNAL   Genes Dev. 2009 Mar 15. 23(6):694-707.
  PUBMED    19299559
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2009 Mar 15. 23(6):694-707."
COMMENT     Sequence Label: E12-cTAP
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(72..245)
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     CDS             complement(246..419)
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             complement(459..479)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(507..584)
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 ctggcgaatt cctacgtacc accacactgg gatcctcatc aggttgactt ccccgcggaa
       61 ttcgcgtcta ctttcggcgc ctgagcacca tttagctttt tagcttctgc taaaaggtta
      121 gcgctttggc ttgggtcatc ttttaaactt tggatgaagg cgtttcgttg ttcttcgttt
      181 aagttaggta aatgtaagat ctcatagaac gcgttttgtt gttctttgtt gaatttgttg
      241 tctactttcg gcgcctgagc atcatttagc tttttagctt ctgctaaaag gttagcgctt
      301 tggcttgggt catcttttaa actttggatg aaggcgtttc gttgttcttc gtttaagtta
      361 ggtaaatgta agatctcata gaacgcgttt tgttgttctt tgttgaattt gttgtccacg
      421 gcttcatcgt gttgcgcaag agccgcggtt ttgagctcac cctgaaaata caaattctcg
      481 ctagcagtag ttggaatatc ataatcaagt gccccggagg atgagatttt cttaaagcgg
      541 ttggctgctg agacggctat gaaattcttt ttccatcttc tcttttccat ggatcccagg
      601 tgcccggctg ggttgtgggc ctcacccagg cccgggtggg cggctgacag ggccagctgt
      661 gggtccccga ccacgccaga caccttctcc tcctcc
//
