LOCUS       sequence-267690-        5130 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human BP1 fragment of SREBF1 in vector for bacterial expression of 
            GST fusion protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Yang F, Vought BW, Satterlee JS, Walker AK, Jim Sun ZY, Watts JL, 
            DeBeaumont R, Saito RM, Hyberts SG, Yang S, Macol C, Iyer L, Tjian 
            R, van den Heuvel S, Hart AC, Wagner G, Naar AM
  TITLE     An ARC/Mediator subunit required for SREBP control of cholesterol 
            and lipid homeostasis.
  JOURNAL   Nature. 2006 Aug 10;442(7103):700-4. doi: 10.1038/nature04942. Epub 
            2006 Jun 21.
  PUBMED    16799563
REFERENCE   2  (bases 1 to 5130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature04942"; journalName: "Nature"; date: "2006-08-10- 
            10"; volume: "442"; issue: "7103"; pages: "700-4"
COMMENT     Sequence Label: pGEX-2TKN-bp1
FEATURES             Location/Qualifiers
     source          1..5130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        18..95
                     /gene="lacI"
                     /label=lacI promoter
     CDS             96..1178
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(115..134)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(1227..1232,1233..1250,1251..1257)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1265..1281
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1289..1305
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1289..1305
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1301..1828
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGISLSTARCTNASGVR
                     QPSEAVVWLCRS"
     primer_bind     complement(1321..1338)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1321..1337)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1330..1352)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        join(1930..1935,1936..1951,1952..1958)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1966..1982
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             2005..2658
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     2616..2638
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             2665..2682
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(2927..2949)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3094..3112)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3180..3284
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3285..3353,3354..4145)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3503..3522)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4316..4904
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4805..4824
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5058..5075
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cacaccgcat aaattccgac accatcgaat ggcgcaaaac ctttcgcggt atggcatgat
       61 agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg ttatacgatg
      121 tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc
      181 acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc
      241 ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct
      301 ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc
      361 aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag
      421 cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg
      481 atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg
      541 atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac
      601 tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat
      661 taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc
      721 aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa
      781 ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga
      841 tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct
      901 cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca
      961 tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc
     1021 agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca
     1081 ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     1141 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtaagt
     1201 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     1261 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacggattc
     1321 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     1381 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     1441 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc
     1501 agaagcggtg ccggaaagct ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt
     1561 cccctcaaac tggcagatgc acggttacga tgcgcccatc tacaccaacg tgacctatcc
     1621 cattacggtc aatccgccgt ttgttcccac ggagaatccg acgggttgtt actcgctcac
     1681 atttaatgtt gatgaaagct ggctacagga aggccagacg cgaattattt ttgatggcgt
     1741 tggaattagc ttatcgactg cacggtgcac caatgcttct ggcgtcaggc agccatcgga
     1801 agctgtggta tggctgtgca ggtcgtaaat cactgcataa ttcgtgtcgc tcaaggcgca
     1861 ctcccgttct ggataatgtt ttttgcgccg acatcataac ggttctggca aatattctga
     1921 aatgagctgt tgacaattaa tcatcggctc gtataatgtg tggaattgtg agcggataac
     1981 aatttcacac aggaaacagt attcatgtcc cctatactag gttattggaa aattaagggc
     2041 cttgtgcaac ccactcgact tcttttggaa tatcttgaag aaaaatatga agagcatttg
     2101 tatgagcgcg atgaaggtga taaatggcga aacaaaaagt ttgaattggg tttggagttt
     2161 cccaatcttc cttattatat tgatggtgat gttaaattaa cacagtctat ggccatcata
     2221 cgttatatag ctgacaagca caacatgttg ggtggttgtc caaaagagcg tgcagagatt
     2281 tcaatgcttg aaggagcggt tttggatatt agatacggtg tttcgagaat tgcatatagt
     2341 aaagactttg aaactctcaa agttgatttt cttagcaagc tacctgaaat gctgaaaatg
     2401 ttcgaagatc gtttatgtca taaaacatat ttaaatggtg atcatgtaac ccatcctgac
     2461 ttcatgttgt atgacgctct tgatgttgtt ttatacatgg acccaatgtg cctggatgcg
     2521 ttcccaaaat tagtttgttt taaaaaacgt attgaagcta tcccacaaat tgataagtac
     2581 ttgaaatcca gcaagtatat agcatggcct ttgcagggct ggcaagccac gtttggtggt
     2641 ggcgaccatc ctccaaaatc ggatctggtt ccgcgtggat ctcgtcgtgc atctgttgga
     2701 tccatggcgc atatggacga gccacccttc agcgaggcgg ctttggagca ggcgctgggc
     2761 gagccgtgcg atctggacgc ggcgctgctg accgacatcg aagacatgct tcagcttatc
     2821 aacaaccaag acagtgactt ccctggccta tttgacccac cctagtagga attcatcgtg
     2881 actgactgac gatctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     2941 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     3001 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
     3061 atagcggagt gtataattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
     3121 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     3181 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     3241 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     3301 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     3361 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     3421 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     3481 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
     3541 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     3601 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     3661 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     3721 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     3781 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa
     3841 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     3901 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     3961 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     4021 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     4081 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     4141 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     4201 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     4261 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     4321 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     4381 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     4441 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     4501 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     4561 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     4621 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     4681 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     4741 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     4801 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     4861 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     4921 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     4981 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     5041 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     5101 attttctcct tacgcatctg tgcggtattt
//
