LOCUS       sequence-267691-       10816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of circular permutant of nSpyCas9 at amino acid position 
            1010 encoding truncated human cytosine deaminase, hAIDx, at the 
            C-terminus. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10816)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 10816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: 1010-AIDx-CP
FEATURES             Location/Qualifiers
     source          1..10816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        99..478
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        479..682
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     633..653
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     727..746
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        727..745
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4963..4983
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4999..5019
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5641..5688
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5689..5712
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5722..5778
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(6271..6288)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6277..6501
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6547..6975
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6634..6653)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6844..6865
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6984..7004)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6989..7318
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7169..7304
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7231..7250
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7385..8179
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7439..7458)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8049..8068
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8353..8474
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8390..8409)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8444..8463
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8523..8539)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8523..8539)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8536..8558)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8547..8563
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8571..8577,8578..8595,8596..8601))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8616..8637
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8754..8771)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8925..9513)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9005..9024)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9684..10475,10476..10544))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10307..10326
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10545..10649)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10724..10743)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
       61 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
      121 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      181 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      241 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      301 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      361 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      421 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      481 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      541 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      601 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      661 ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt actggcttat
      721 cgaaattaat acgactcact atagggagac ccaagctggc tagcgtttaa acttaagctt
      781 ggtaccgcca ccatgaatgg tgactataaa gtgtatgatg ttcgtaagat gatcgcaaag
      841 tccgagcagg agattggcaa ggcaaccgct aagtattttt tttacagcaa catcatgaac
      901 ttctttaaga ccgagattac cctggccaac ggtgaaatcc gtaaacgccc gctgattgaa
      961 accaacggtg aaaccggcga aatcgtgtgg gacaagggcc gtgattttgc gaccgtgcgc
     1021 aaagtgctgt ccatgccgca ggtgaacatt gtgaagaaaa ccgaggtgca gaccggcggt
     1081 ttcagcaagg aaagcattct gccgaaacgt aacagcgaca agctgatcgc ccgcaagaaa
     1141 gattgggacc cgaagaaata cggcggtttc gatagcccga ccgtggctta tagcgtgctg
     1201 gtggtggcca aagtggagaa gggtaaaagc aagaaactga agagcgtgaa agaactgctg
     1261 ggcatcacca ttatggagcg tagcagcttc gaaaagaacc cgatcgactt tctggaggcg
     1321 aagggttaca aggaagtgaa gaaagatctg atcattaagc tgccgaaata cagcctgttt
     1381 gagctggaaa atggtcgtaa acgtatgctg gccagcgccg gcgagctgca aaagggcaac
     1441 gaactggcgc tgccgagcaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag
     1501 ctgaaaggta gcccggagga caacgaacag aagcagctgt ttgtggagca gcacaaacac
     1561 tatctggatg agatcattga acagatcagc gagttcagca agcgtgtgat tctggcggac
     1621 gctaacctgg ataaagtgct gagcgcctac aacaaacacc gtgacaagcc gatccgcgag
     1681 caggcggaaa acatcattca cctgttcacc ctgaccaacc tgggtgcgcc ggccgcgttc
     1741 aagtactttg acaccaccat tgatcgtaaa cgctatacca gcaccaagga ggtgctggat
     1801 gctaccctga tccaccagag cattaccggc ctgtacgaaa cccgtattga tctgagccag
     1861 ctgggcggtg acggcggtag cggcggtagc ggcggtagcg gcggtagcgg cggtagcggc
     1921 ggtagcggcg gtgacaagaa atatagcatc ggtctggcga ttggcaccaa cagcgtgggt
     1981 tgggctgtga tcaccgatga gtacaaggtg ccgagcaaga agttcaaggt gctgggcaac
     2041 accgaccgcc acagcatcaa gaaaaacctg attggtgccc tgctgtttga tagcggtgaa
     2101 accgccgagg ccacccgtct gaagcgcacc gcccgtcgcc gttacacccg tcgtaaaaac
     2161 cgtatctgct acctgcaaga gattttcagc aacgaaatgg ccaaagtgga cgatagcttc
     2221 tttcaccgcc tggaggagag cttcctggtg gaggaagaca agaaacacga gcgtcacccg
     2281 atctttggca acattgtgga tgaggtggct taccacgaaa agtatccgac catctaccac
     2341 ctgcgtaaga aactggtgga tagcaccgac aaggccgatc tgcgcctgat ctatctggct
     2401 ctggcccaca tgatcaagtt ccgtggtcac tttctgatcg agggcgacct gaacccggat
     2461 aacagcgatg tggacaagct gttcatccag ctggtgcaga cctacaacca gctgtttgag
     2521 gaaaacccga ttaacgccag cggtgtggac gcgaaggcta tcctgagcgc gcgtctgagc
     2581 aaaagccgcc gtctggagaa cctgatcgcg cagctgccgg gtgaaaagaa aaacggtctg
     2641 tttggcaacc tgattgctct gagcctgggc ctgaccccga acttcaaaag caactttgac
     2701 ctggcggagg atgctaagct gcaactgagc aaggatacct atgacgatga cctggacaac
     2761 ctgctggcgc agatcggcga ccagtacgct gatctgttcc tggctgccaa gaacctgagc
     2821 gatgcgatcc tgctgagcga cattctgcgt gtgaacaccg agattaccaa ggccccgctg
     2881 agcgcgagca tgatcaaacg ctatgatgaa caccaccagg acctgaccct gctgaaagct
     2941 ctggtgcgtc agcagctgcc ggagaagtac aaagaaatct tctttgacca gagcaagaac
     3001 ggttacgccg gctatattga tggcggtgcg agccaggagg agttctacaa gtttatcaaa
     3061 ccgattctgg agaaaatgga cggcaccgag gaactgctgg tgaagctgaa ccgtgaagat
     3121 ctgctgcgta aacagcgcac cttcgacaac ggtagcatcc cgcaccagat tcacctgggt
     3181 gagctgcacg ccatcctgcg ccgtcaggaa gatttctacc cgtttctgaa ggacaaccgt
     3241 gagaagatcg aaaaaattct gaccttccgc attccgtact atgtgggtcc gctggctcgt
     3301 ggcaacagcc gctttgcctg gatgacccgc aagagcgagg aaaccatcac cccgtggaac
     3361 ttcgaggaag tggtggacaa aggcgccagc gcgcagagct tcattgagcg tatgaccaac
     3421 tttgataaga acctgccgaa cgaaaaagtg ctgccgaagc acagcctgct gtacgagtat
     3481 ttcaccgtgt acaacgaact gaccaaggtg aaatatgtga ccgagggtat gcgcaagccg
     3541 gccttcctga gcggcgaaca gaagaaagcg atcgtggacc tgctgtttaa aaccaaccgt
     3601 aaggtgaccg tgaagcagct gaaagaggat tatttcaaga aaattgaatg ctttgacagc
     3661 gtggagatca gcggtgtgga agatcgtttt aacgcgagcc tgggcaccta ccacgacctg
     3721 ctgaaaatca ttaaggataa agacttcctg gataacgagg aaaacgagga catcctggaa
     3781 gatattgtgc tgaccctgac cctgtttgag gaccgtgaaa tgatcgagga acgcctgaag
     3841 acctatgccc acctgttcga tgacaaagtg atgaaacagc tgaagcgccg tcgctacacc
     3901 ggttggggcc gtctgagccg caaactgatc aacggtattc gcgataaaca gagcggcaag
     3961 accatcctgg attttctgaa gagcgacggt ttcgcgaacc gtaactttat gcagctgatt
     4021 cacgatgaca gcctgacctt caaagaggac atccagaagg cgcaggtgag cggtcagggc
     4081 gatagcctgc acgaacatat tgctaacctg gccggtagcc cggccatcaa gaagggcatc
     4141 ctgcaaaccg tgaaggtggt ggacgagctg gtgaaagtga tgggtcgtca caagccggaa
     4201 aacatcgtga ttgagatggc ccgcgaaaac cagaccaccc agaaaggcca gaagaacagc
     4261 cgtgagcgca tgaagcgtat cgaggaaggc atcaaggaac tgggcagcca gatcctgaag
     4321 gagcacccgg tggaaaacac ccagctgcaa aacgagaaac tgtacctgta ctacctgcaa
     4381 aacggtcgtg acatgtatgt ggatcaggaa ctggacatta accgcctgag cgattatgac
     4441 gtggatgcga tcgtgccgca gagcttcctg aaggatgaca gcattgacaa caaagtgctg
     4501 acccgtagcg ataaaaaccg cggcaagagc gacaacgtgc cgagcgagga agtggtgaag
     4561 aaaatgaaaa actactggcg tcagctgctg aacgccaaac tgatcaccca gcgtaagttt
     4621 gacaacctga ccaaagctga gcgcggcggt ctgagcgaac tggataaagc cggtttcatt
     4681 aagcgtcagc tggtggaaac ccgccagatc accaagcacg tggcgcagat tctggacagc
     4741 cgcatgaaca ccaagtatga cgagaacgat aaactgatcc gtgaagtgaa agtgatcacc
     4801 ctgaagagca aactggtgag cgattttcgt aaagacttcc agttttacaa ggtgcgcgag
     4861 atcaacaact accaccacgc tcacgacgcc tatctgaacg cggtggtggg caccgccctg
     4921 atcaagaagt acccgaagct ggagagcgag ttcgtgtacg gcccgaagaa aaagcgtaaa
     4981 gtgggtagca gcggcagccc gaaaaagaaa cgcaaagtgg gtagcgacgc tctggatgac
     5041 ttcgacctgg atatgctggg cagcgacgcc ctggatgact ttggcggtgg cagcatggat
     5101 agcctgctga tgaaccgtcg caaattcctg tatcagttta agaacgtgcg ttgggctaaa
     5161 ggccgtcgcg aaacctacct gtgctatgtg gtgaaacgtc gcgatagcgc caccagcttc
     5221 agcctggact ttggttacct gcgcaacaag aacggctgcc acgtggagct gctgttcctg
     5281 cgttacatta gcgactggga cctggaccca ggccgttgct atcgcgtgac ctggtttacc
     5341 agctggagcc cgtgctacga ttgcgctcgt catgtggcgg acttcctgcg tggtaaccca
     5401 aacctgagcc tgcgtatctt caccgcgcgc ctgtactttt gcgaggaccg taaggctgag
     5461 ccggaaggtc tgcgtcgcct gcaccgtgcg ggtgtgcaga tcgcgatcat gaccttcaaa
     5521 gattactttt attgctggaa caccttcgtg gagaaccacg aacgcacctt taaggcctgg
     5581 gagggtctgc acgaaaacag cgtgcgtctg agccgccagc tgcgccgcat cctgctgcca
     5641 aagcgtccgg cggctaccaa gaaagcgggc caggctaaga aaaagaaaga ctacaaagat
     5701 gacgatgaca agggtagcgg cgctaccaac ttcagcctgc tgaagcaggc gggcgacgtg
     5761 gaggaaaatc cgggcccgat ggctaaaccg ctgagccagg aggaaagcac cctgatcgag
     5821 cgcgctaccg ccaccattaa cagcatcccg attagcgaag actatagcgt ggccagcgcc
     5881 gcgctgagca gcgatggtcg tattttcacc ggcgtgaacg tgtaccactt taccggtggc
     5941 ccgtgcgccg agctggtggt gctgggcacc gccgccgccg ccgccgccgg caacctgacc
     6001 tgcatcgtgg cgattggtaa cgaaaatcgt ggcatcctga gcccgtgcgg ccgttgccgc
     6061 caggtgctgc tggacctgca tccgggcatc aaggcgattg tgaaggatag cgacggccaa
     6121 ccgaccgccg tgggtattcg tgaactgctg ccatccggct atgtttggga gggctaagga
     6181 tccactagtc cagtgtggtg gaattctgca gatatccagc acagtggcgg ccgctcgagt
     6241 ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     6301 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     6361 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     6421 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     6481 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     6541 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     6601 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     6661 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     6721 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     6781 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     6841 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     6901 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     6961 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     7021 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     7081 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     7141 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     7201 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     7261 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     7321 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt
     7381 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     7441 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     7501 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     7561 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     7621 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     7681 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     7741 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     7801 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     7861 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     7921 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     7981 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     8041 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     8101 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     8161 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     8221 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     8281 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     8341 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     8401 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     8461 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     8521 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     8581 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     8641 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     8701 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     8761 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     8821 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     8881 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     8941 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     9001 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     9061 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     9121 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9181 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9241 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9301 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9361 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9421 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     9481 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     9541 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     9601 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     9661 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     9721 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     9781 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     9841 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     9901 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     9961 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    10021 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    10081 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    10141 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    10201 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    10261 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    10321 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    10381 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    10441 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    10501 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    10561 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    10621 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    10681 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
    10741 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
    10801 cgagcaaaat ttaagc
//
